Two New Alkaloids from Fusarium tricinctum SYPF 7082,an Endophyte from the Root of Panax notoginseng

Panax notoginseng(Araliaceae)is a famous traditional Chinese medicine mainly cultivated in Yunnan and Guangxi provinces of China.Two new alkaloids,rigidiusculamide E(1)and[-(a-oxyisohexanoyl-N-methyl-leucyl)2-](2),together with two known ones,(-)-oxysporidinone(3)and(-)-4,60-anhydrooxysporidinone(4)were isolated from the mycelia culture of Fusarium tricinctum SYPF 7082,an endophytic fungus obtained from the healthy root of P.notoginseng.Their structures were determined on the basis of extensive spectroscopic analyses.Compounds 1-4 were tested for their inhibitory effects against NO production on Murine macrophage cell line,and the new compound 2 showed significant inhibitory activity on NO production with the IC_(50)value of 18.10±0.16μM.

Anti‑inflammatory and Cytotoxic Triterpenes from the Rot Roots of Panax notoginseng

Four new protopanaxatriol-type triterpenes(1-2)and glucosides(3-4),were isolated from the rot roots of Panax notoginseng(Burk.)Chen,along with four known ones(5-8).Their structures were elucidated on the basis of extensive spectroscopic analysis(HRESIMS,NMR,UV,IR,and OR)and acidic hydrolysis.The possible transformation pathway of these compounds were also speculated from ginsenoside Rg_(1).Compound 1,with a uniqueα,β-unsaturated ketene in its side chain,showed significant inhibitory effects against NO production on Murine macrophage cells(IC_(50)=4.12±0.20μM)and comparable cytotoxicities against five human cancer cell lines(myeloid leukemia HL-60,lung cancer A-549 cells,hepatocellular carcinoma SMMC7721,breast cancer MCF-7,and colon cancer SW480)to positive control,cisplatin(DDP).

Polyhydroxylated eudesmane sesquiterpenoids and sesquiterpenoid glucoside from the flower buds of Tussilago farfara

Chemical fractionation of the n-BuOH partition,which was generated from the EtOH extract of the flower buds of Tussilago farfara,afforded a series of polar constituents including four new sesquiterpenoids(1-4),one new sesquiterpenoid glucoside(5)and one known analogue(6)of the eudesmane type,as well as five known quinic acid derivatives(7-11).Structures of the new compounds were unambiguously characterized by detailed spectroscopic analyses,with their absolute configurations being established by A-ray crystallography,electronic circular dichroism(ECD)calculation and induced ECD experiments.The inhibitory effect of all the isolates against LPS-induced NO production in murine RAW264.7 macrophages was evaluated,with isochlorogenic acid A(7)showing significant inhibitory activity.

Bioactive sesquineolignans from the twigs of Litsea cubeba

In a continuing search for biological natural products with structure diversity from traditional Chinese herbs,five new sesquineolignans(1−5)were isolated from an ethyl acetate extract of the twigs of Litsea cubeba.Their structures were elucidated based on MS,1D and 2D NMR spectroscopic data,as well as experimental electronic circular dichroism(ECD)spectra.Compounds 1−5 showed moderate inhibitory effects against LPS-induced NO production in RAW264.7 macrophages,with IC50 values of 16.2,20.2,22.1,15.1,and 16.6μmol·L^(−1),respectively.

Chemical constituents from the aerial parts of Waltheria indica Linn.

A phytochemical investigation on the aerial parts of Waltheria indica Linn.led to the isolation of 16 compounds,including five terpenoids(1–5),four coumarins(6–9),six flavonoids(10–15),and one phenylpropanoid glycoside(16).The structures of these compounds were identified by spectroscopic data analysis and comparison with those reported in the literatures.Except for 12 and 15,all the compounds were isolated from W.indica for the first time.Moreover,coumarins were firstly reported to be obtained from this herb.The NO production inhibitory activities of all the isolated compounds in LPS-induced BV-2 cells were also presented.