The settlement substrates of nona-porous abalones （Haliotis diversicolor supertexta） are covered with biofilms in which several types of microorganisms coexist and interact. These microorganisms are usually importan...The settlement substrates of nona-porous abalones （Haliotis diversicolor supertexta） are covered with biofilms in which several types of microorganisms coexist and interact. These microorganisms are usually important causes of juvenile abalone disease as well as organisms useful in promoting abalones’ adhesion. The bacterial community structure of the biofilms remains unclear. The aim of this research was to determine the genetic diversity and phylogenetic affiliation of the biofilm bacteria. Total DNA of bacteria in biofilms was extracted, and 16S rRNA gene clone library was constructed using the primers specific for the domain bacteria. Subsequently, 30 randomly selected positive clones were screened by PCR-restriction fragment length polymorphism （PCR-RFLP） analysis, and resulted in 15 different RFLP patterns. Sequences analysis of representatives from each unique RFLP type revealed high genetic diversity in the bacterial populations. These sequences fell into nine major lineages of the bacterial domains： α-, β-, γ-and δ-subdivisions of the Proteobacteria; Planctomycete, Actinobacteria, Firmicutes, V errucomicrobium spp., and CytophagaFlexibacter-Bacteroides spp. Phylogenetic analysis indicated that the dominant phylotypes were most closely related to environmental and clinical Burkholderia cepacia of the β-Proteobacteria, and Roseobacteria of the α-Proteobacteria.展开更多
研究了阴离子表面活性剂(SDBS)、非离子表面活性剂(Lutensol TO 10)和多元醇(乙二醇,甘油和1,2-丙二醇)对P(St-co-MAA)纳米色素分散液稳定性的影响。结果表明,SDBS和Lutensol TO 10质量浓度分别为20 g/L和30 g/L时能够显著提高P(St-co-M...研究了阴离子表面活性剂(SDBS)、非离子表面活性剂(Lutensol TO 10)和多元醇(乙二醇,甘油和1,2-丙二醇)对P(St-co-MAA)纳米色素分散液稳定性的影响。结果表明,SDBS和Lutensol TO 10质量浓度分别为20 g/L和30 g/L时能够显著提高P(St-co-MAA)纳米色素分散液的稳定性,但随着其质量浓度继续增加,分散液稳定性下降。随着乙二醇、甘油和1,2-丙二醇质量浓度的升高,P(St-co-MAA)纳米色素分散液稳定性不断升高。其中,甘油对纳米色素分散液稳定性的提升效果最佳。展开更多
基金The Natural Science Foundation of Fujian Province of China under contract Nos 2005k055 and B0740012the Innovative Research Team under contract No 2006A001Science Foundation of Jimei University, China
文摘The settlement substrates of nona-porous abalones （Haliotis diversicolor supertexta） are covered with biofilms in which several types of microorganisms coexist and interact. These microorganisms are usually important causes of juvenile abalone disease as well as organisms useful in promoting abalones’ adhesion. The bacterial community structure of the biofilms remains unclear. The aim of this research was to determine the genetic diversity and phylogenetic affiliation of the biofilm bacteria. Total DNA of bacteria in biofilms was extracted, and 16S rRNA gene clone library was constructed using the primers specific for the domain bacteria. Subsequently, 30 randomly selected positive clones were screened by PCR-restriction fragment length polymorphism （PCR-RFLP） analysis, and resulted in 15 different RFLP patterns. Sequences analysis of representatives from each unique RFLP type revealed high genetic diversity in the bacterial populations. These sequences fell into nine major lineages of the bacterial domains： α-, β-, γ-and δ-subdivisions of the Proteobacteria; Planctomycete, Actinobacteria, Firmicutes, V errucomicrobium spp., and CytophagaFlexibacter-Bacteroides spp. Phylogenetic analysis indicated that the dominant phylotypes were most closely related to environmental and clinical Burkholderia cepacia of the β-Proteobacteria, and Roseobacteria of the α-Proteobacteria.
文摘研究了阴离子表面活性剂(SDBS)、非离子表面活性剂(Lutensol TO 10)和多元醇(乙二醇,甘油和1,2-丙二醇)对P(St-co-MAA)纳米色素分散液稳定性的影响。结果表明,SDBS和Lutensol TO 10质量浓度分别为20 g/L和30 g/L时能够显著提高P(St-co-MAA)纳米色素分散液的稳定性,但随着其质量浓度继续增加,分散液稳定性下降。随着乙二醇、甘油和1,2-丙二醇质量浓度的升高,P(St-co-MAA)纳米色素分散液稳定性不断升高。其中,甘油对纳米色素分散液稳定性的提升效果最佳。
