This paper presents a new programming paradigm named Notification Oriented Paradigm (NOP) and analyses performance aspects of NOP programs by means of an experiment. NOP provides a new manner to conceive, structure, a...This paper presents a new programming paradigm named Notification Oriented Paradigm (NOP) and analyses performance aspects of NOP programs by means of an experiment. NOP provides a new manner to conceive, structure, and execute software, which allows better performance, causal-knowledge organization, and entity decoupling than standard solutions based upon current paradigms. These paradigms are essentially Imperative Paradigm (IP) and Declarative Paradigm (DP). In short, DP solutions are considered easier to use than IP solutions thanks to the concept of high-level programming. However, they are considered slower to execute and lesser flexible to program than IP. Anyway, both paradigms present similar drawbacks like causal-evaluation redundancies and strongly coupled entities, which decrease software performance and processing distribution feasibility. These problems exist due to an orientation to monolithic inference mechanism based upon sequential evaluation by means of searches over passive computational entities. NOP proposes another manner to structure software and make its inferences, which is based upon small, smart, and decoupled collaborative entities whose interaction happen by means of precise notifications. This paper discusses NOP as a paradigm and presents certain comparison of NOP against IP. Actually, performance is evaluated by means of IP and NOP programs with respect to a same application, which allow demonstrating NOP superiority.展开更多
Cel proliferation is a fundamental event essential for plant organogenesis and contributes greatly to the final organ size. Although the control of cel proliferation in plants has been extensively studied, how the pla...Cel proliferation is a fundamental event essential for plant organogenesis and contributes greatly to the final organ size. Although the control of cel proliferation in plants has been extensively studied, how the plant sets the cel number required for a single organ is largely elusive. Here, we describe the Arabidopsis SMALL ORGAN 4 (SMO4) that functions in the regulation of cell proliferation rate and thus final organ size. The smo4 mutant exhibits a reduced size of organs due to the decreased cell number, and further analysis reveals that such phenotype results from a retardation of the cell cycle progression during organ development. SMO4 encodes a homolog of NUCLEOLAR PROTEIN 53 (NOP53) in Saccharomy-ces cerevisiae and is expressed primarily in tissues undergoing cel proliferation. Nevertheless, further complementation tests show that SMO4 could not rescue the lethal defect of NOP53 mutant of S. cerevisiae. These results define SMO4 as an important regulator of cell proliferation during organ growth and suggest that SMO4 might have been evolutionarily divergent from NOP53.展开更多
The female gametophyte is crucial for sexual reproduction of higher plants, yet little is known about the molecular mechanisms underlying its development. Here, we report that Arabidopsis thaliana NOP10 （AtNOP10） is...The female gametophyte is crucial for sexual reproduction of higher plants, yet little is known about the molecular mechanisms underlying its development. Here, we report that Arabidopsis thaliana NOP10 （AtNOP10） is required for female gametophyte formation. AtNOP10 was expressed predominantly in the seedling and reproductive tissues, including anthers, pollen grains, and ovules. Mutations in AtNOP10 interrupted mitosis of the functional megaspore during early development and prevented polar nuclear fusion in the embryo sacs. AtNOP10 shares a high level of amino acid sequence similarity with Saccharornycescerevisiae （yeast） NOPIo （ScNOP1o）, an important compo- nent of the H/ACA small nucleolar ribonucleoprotein particles （H/ACA snoRNPs） implicated in 18S rRNA synthe- sis and rRNA pseudouridylation. Heterologous expression of ScNOP10 complemented the mutant phenotype of Atnop10. Thus, AtNOPIo influences functional megaspore mitosis and polar nuclear fusion during gametophyte formation in Arabidopsis.展开更多
文摘This paper presents a new programming paradigm named Notification Oriented Paradigm (NOP) and analyses performance aspects of NOP programs by means of an experiment. NOP provides a new manner to conceive, structure, and execute software, which allows better performance, causal-knowledge organization, and entity decoupling than standard solutions based upon current paradigms. These paradigms are essentially Imperative Paradigm (IP) and Declarative Paradigm (DP). In short, DP solutions are considered easier to use than IP solutions thanks to the concept of high-level programming. However, they are considered slower to execute and lesser flexible to program than IP. Anyway, both paradigms present similar drawbacks like causal-evaluation redundancies and strongly coupled entities, which decrease software performance and processing distribution feasibility. These problems exist due to an orientation to monolithic inference mechanism based upon sequential evaluation by means of searches over passive computational entities. NOP proposes another manner to structure software and make its inferences, which is based upon small, smart, and decoupled collaborative entities whose interaction happen by means of precise notifications. This paper discusses NOP as a paradigm and presents certain comparison of NOP against IP. Actually, performance is evaluated by means of IP and NOP programs with respect to a same application, which allow demonstrating NOP superiority.
基金supported by grants from the National Natural Science Foundation of China (30800599 and 30671132)
文摘Cel proliferation is a fundamental event essential for plant organogenesis and contributes greatly to the final organ size. Although the control of cel proliferation in plants has been extensively studied, how the plant sets the cel number required for a single organ is largely elusive. Here, we describe the Arabidopsis SMALL ORGAN 4 (SMO4) that functions in the regulation of cell proliferation rate and thus final organ size. The smo4 mutant exhibits a reduced size of organs due to the decreased cell number, and further analysis reveals that such phenotype results from a retardation of the cell cycle progression during organ development. SMO4 encodes a homolog of NUCLEOLAR PROTEIN 53 (NOP53) in Saccharomy-ces cerevisiae and is expressed primarily in tissues undergoing cel proliferation. Nevertheless, further complementation tests show that SMO4 could not rescue the lethal defect of NOP53 mutant of S. cerevisiae. These results define SMO4 as an important regulator of cell proliferation during organ growth and suggest that SMO4 might have been evolutionarily divergent from NOP53.
基金financially supported by the National Natural Science Foundation (31770353 and 30530060)
文摘The female gametophyte is crucial for sexual reproduction of higher plants, yet little is known about the molecular mechanisms underlying its development. Here, we report that Arabidopsis thaliana NOP10 （AtNOP10） is required for female gametophyte formation. AtNOP10 was expressed predominantly in the seedling and reproductive tissues, including anthers, pollen grains, and ovules. Mutations in AtNOP10 interrupted mitosis of the functional megaspore during early development and prevented polar nuclear fusion in the embryo sacs. AtNOP10 shares a high level of amino acid sequence similarity with Saccharornycescerevisiae （yeast） NOPIo （ScNOP1o）, an important compo- nent of the H/ACA small nucleolar ribonucleoprotein particles （H/ACA snoRNPs） implicated in 18S rRNA synthe- sis and rRNA pseudouridylation. Heterologous expression of ScNOP10 complemented the mutant phenotype of Atnop10. Thus, AtNOPIo influences functional megaspore mitosis and polar nuclear fusion during gametophyte formation in Arabidopsis.
