Background: Supplementing farm animals diet with functional ingredients may improve the nutritional quality of meat products.Diet composition has been also demonstrated to influence the gene expression with effect on ...Background: Supplementing farm animals diet with functional ingredients may improve the nutritional quality of meat products.Diet composition has been also demonstrated to influence the gene expression with effect on biological processes and pathways.However,the knowledge on the effect of nutrients at the molecular level is scant.In particular,studies on the effects of antioxidants and polyphenols dietary supplementation have been investigated mainly in rodents,and only scarcely in farm animals so far.RNA-Seq with next-generation sequencing is increasingly the method of choice for studying changes in the transcriptome and it has been recently employed also in pig nutrigenomics studies to identify diet-induced changes in gene expression.The present study aimed to investigate the effect of diets enriched with functional ingredients(linseed,vitamin E and plant extracts) on the transcriptome of pig Longissimus thoracis to elucidate the role of these compounds in influencing genes involved in muscle physiology and metabolism compared to a standard diet.Results: Eight hundred ninety-three significant differentially expressed genes(DEGs)(FDR adjusted P-value ≤ 0.05)were detected by RNA-Seq analysis in the three diet comparisons(D2-D1,D3-D1,D4-D1).The functional analysis of DEGs showed that the diet enriched with n-3 PUFA from linseed(D2) mostly downregulated genes in pathways and biological processes(BPs) related to muscle development,contraction,and glycogen metabolism compared to the standard diet.The diet supplemented with linseed and vitamin E/Selenium(D3) showed to mostly downregulate genes linked to oxidative phosphorylation.Only few genes involved in extracellular matrix(ECM)organization were upregulated by the D3.Finally,the comparison D4-D1 showed that the diet supplemented with linseed and plant extracts(D4) upregulated the majority of genes compared to D1 that were involved in a complex network of pathways and BPs all connected by hub genes.In particular,IGF2 was a hub gene connecting protein metabolism,ECM organization,immune system and lipid biosynthesis pathways.Conclusion: The supplementation of pig diet with n-3 PUFA from linseed,antioxidants and plant-derived polyphenols can influence the expression of a relevant number of genes in Longissimus thoracis muscle that are involved in a variety of biochemical pathways linked to muscle function and metabolism.展开更多
目的:评价补充不同维生素对预防早产儿支气管肺发育不良(BPD)的疗效。方法:检索中国知网(CNKI)、万方(Wan fang)、维普网(cqvip)、PubMed、Cochrane Library、Web of Science数据库,纳入关于补充维生素预防早产儿BPD的随机对照试验(RCT...目的:评价补充不同维生素对预防早产儿支气管肺发育不良(BPD)的疗效。方法:检索中国知网(CNKI)、万方(Wan fang)、维普网(cqvip)、PubMed、Cochrane Library、Web of Science数据库,纳入关于补充维生素预防早产儿BPD的随机对照试验(RCTs),筛选符合纳入标准的RCTs,运用Cochrane偏倚风险评估工具进行文献质量评价,通过贝叶斯网状meta分析,分析比较不同维生素预防BPD的疗效。结果:共纳入21篇RCTs,(3188例早产儿)。与安慰剂组相比,中等剂量补充维生素D(400~800IU/d,OR=0.41,95%Cl[0.18,0.90])、小剂量补充维生素A(<1333IU/kg/d,OR=0.50,95%Cl[0.25,0.95])与BPD发生率降低相关。小剂量补充维生素D(<400IU/d,OR=12.36,95%Cl[1.00,600.21])可能与早产儿死亡率增加相关。小剂量补充维生素E(OR=0.59,95%Cl[0.23,1.36]),大剂量补充维生素E(OR=0.74,95%Cl[0.14,3.64])置信区间较宽,2项与预防早产儿BPD关系不大。结论:补充中等剂量维生素D、小剂量维生素A可有效预防早产儿BPD发生。小剂量补充维生素D可能增加早产儿死亡率,提示早产儿补充维生素D时应予足量补充。目前尚未充分证实维生素E在预防BPD方面的有效性。展开更多
基金funded by Regione Emilia-Romagna POR-FESR 2014-2020"Innovare la filiera suina mediante la valorizzazione di sottoprodotti vegetali e l’impiego di avanzate tecnologie "omiche" e di processo,per la produzione sostenibile di carne e salumi ad impatto positivo sulla salute"-Green Charcuterie grant n.PG/2015/730542
文摘Background: Supplementing farm animals diet with functional ingredients may improve the nutritional quality of meat products.Diet composition has been also demonstrated to influence the gene expression with effect on biological processes and pathways.However,the knowledge on the effect of nutrients at the molecular level is scant.In particular,studies on the effects of antioxidants and polyphenols dietary supplementation have been investigated mainly in rodents,and only scarcely in farm animals so far.RNA-Seq with next-generation sequencing is increasingly the method of choice for studying changes in the transcriptome and it has been recently employed also in pig nutrigenomics studies to identify diet-induced changes in gene expression.The present study aimed to investigate the effect of diets enriched with functional ingredients(linseed,vitamin E and plant extracts) on the transcriptome of pig Longissimus thoracis to elucidate the role of these compounds in influencing genes involved in muscle physiology and metabolism compared to a standard diet.Results: Eight hundred ninety-three significant differentially expressed genes(DEGs)(FDR adjusted P-value ≤ 0.05)were detected by RNA-Seq analysis in the three diet comparisons(D2-D1,D3-D1,D4-D1).The functional analysis of DEGs showed that the diet enriched with n-3 PUFA from linseed(D2) mostly downregulated genes in pathways and biological processes(BPs) related to muscle development,contraction,and glycogen metabolism compared to the standard diet.The diet supplemented with linseed and vitamin E/Selenium(D3) showed to mostly downregulate genes linked to oxidative phosphorylation.Only few genes involved in extracellular matrix(ECM)organization were upregulated by the D3.Finally,the comparison D4-D1 showed that the diet supplemented with linseed and plant extracts(D4) upregulated the majority of genes compared to D1 that were involved in a complex network of pathways and BPs all connected by hub genes.In particular,IGF2 was a hub gene connecting protein metabolism,ECM organization,immune system and lipid biosynthesis pathways.Conclusion: The supplementation of pig diet with n-3 PUFA from linseed,antioxidants and plant-derived polyphenols can influence the expression of a relevant number of genes in Longissimus thoracis muscle that are involved in a variety of biochemical pathways linked to muscle function and metabolism.
文摘目的:评价补充不同维生素对预防早产儿支气管肺发育不良(BPD)的疗效。方法:检索中国知网(CNKI)、万方(Wan fang)、维普网(cqvip)、PubMed、Cochrane Library、Web of Science数据库,纳入关于补充维生素预防早产儿BPD的随机对照试验(RCTs),筛选符合纳入标准的RCTs,运用Cochrane偏倚风险评估工具进行文献质量评价,通过贝叶斯网状meta分析,分析比较不同维生素预防BPD的疗效。结果:共纳入21篇RCTs,(3188例早产儿)。与安慰剂组相比,中等剂量补充维生素D(400~800IU/d,OR=0.41,95%Cl[0.18,0.90])、小剂量补充维生素A(<1333IU/kg/d,OR=0.50,95%Cl[0.25,0.95])与BPD发生率降低相关。小剂量补充维生素D(<400IU/d,OR=12.36,95%Cl[1.00,600.21])可能与早产儿死亡率增加相关。小剂量补充维生素E(OR=0.59,95%Cl[0.23,1.36]),大剂量补充维生素E(OR=0.74,95%Cl[0.14,3.64])置信区间较宽,2项与预防早产儿BPD关系不大。结论:补充中等剂量维生素D、小剂量维生素A可有效预防早产儿BPD发生。小剂量补充维生素D可能增加早产儿死亡率,提示早产儿补充维生素D时应予足量补充。目前尚未充分证实维生素E在预防BPD方面的有效性。
