BACKGROUND The prognostic assessment of patients after surgical resection of gastric cancer(GC)patients is critical.However,the role of the circadian clock gene NPAS2 expression in GC remains unknown.AIM To explore th...BACKGROUND The prognostic assessment of patients after surgical resection of gastric cancer(GC)patients is critical.However,the role of the circadian clock gene NPAS2 expression in GC remains unknown.AIM To explore the relationship between NPAS2 and the survival prognosis of GC patients and clarify its role in evaluating GC prognosis.METHODS The tumor tissues and clinical data of 101 patients with GC were collected retrospectively.Immunohistochemical staining(IHC)was used to detect the expression of NPAS2 protein in GC and adjacent tissues.Univariate and multivariate Cox regression analysis was used to determine the independent prognostic factors of GC,and a nomogram prediction model was established.The receiver operating characteristic(ROC)curve,the ROC area under the curve,the calibration curve,and C-index were used to evaluate the predictive effectiveness of the model.Kaplan Meier analysis was used to compare the risk stratification of subgroups according to the median score in the nomogram model of each patient.RESULTS Microarray IHC analysis showed that the positive rate of NPAS2 protein expression in GC tissues was 65.35%,which was significantly higher than 30.69%in adjacent tissues.The high expression of NPAS2 was correlated with tumor-node-metastasis(TNM)stage(P<0.05),pN stage(P<0.05),metastasis(P<0.05),venous invasion(P<0.05),lymphatic invasion(P<0.05),and lymph node positive(P<0.05)of GC.Kaplan Meier survival analysis showed that the 3-year overall survival(OS)of patients with high NPAS2 expression was significantly shortened(P<0.0001).Univariate and multivariate COX regression analysis showed that TNM stage(P=0.009),metastasis(P=0.009),and NPAS2 expression(P=0.020)were independent prognostic factors of OS in GC patients for 3 years.The nomogram prediction model based on independent prognostic factors has a C-Index of 0.740(95%CI:0.713-0.767).Furthermore,subgroup analysis showed that the 3-year OS time of the high-risk group was significantly lower than that of the low-risk group(P<0.0001).CONCLUSION NPAS2 is highly expressed in GC tissues and is closely related to worse OS in patients.Therefore,the evaluation of NPAS2 expression may be a potential marker for GC prognosis evaluation.Notably,the nomogram model based on NPAS2 can improve the accuracy of GC prognosis prediction and assist clinicians in postoperative patient management and decision-making.展开更多
目的检测生物节律基因NPAS2在结直肠癌组织及相应癌旁组织中的表达情况;明确NPAS2的表达与患者临床病理学参数之间的相关性,分析NPAS2表达与结直肠癌患者5年生存率之间的相关性,明确其在判断结直肠癌患者预后中的作用。方法采用RT-q PC...目的检测生物节律基因NPAS2在结直肠癌组织及相应癌旁组织中的表达情况;明确NPAS2的表达与患者临床病理学参数之间的相关性,分析NPAS2表达与结直肠癌患者5年生存率之间的相关性,明确其在判断结直肠癌患者预后中的作用。方法采用RT-q PCR检测NPAS2 m RNA在40例结直肠癌及癌旁新鲜组织中的表达;采用免疫组织化学SABC法检测NPAS2蛋白在120例肿瘤及癌旁石蜡标本中的表达;利用其中78例随访患者资料,K-M生存法分析NPAS2蛋白表达水平与患者生存率之间的相关性。结果 RT-q PCR结果提示,结直肠癌中NPAS2 m RNA表达显著低于相对应的癌旁组织(P<0.01);免疫组织化学结果提示,NPAS2蛋白表达在结直肠癌组织中的阳性率为19.2%,显著低于癌旁中的62.5%(P<0.05)。NPAS2的低表达与结直肠癌的肿瘤大小、淋巴结转移及TNM分期密切相关(P<0.05),而与患者年龄、性别、分化、浸润深度及远处转移无显著相关性(P>0.05)。K-M生存分析结果表明,NPAS2高表达组患者5年生存率显著高于低表达组,差异具有统计学意义(P<0.01)。结论NPAS2在结直肠癌组织中低表达,且与肿瘤部分恶性生物学行为及患者5年生存率密切相关,提示检测NPAS2的表达对预测结直肠癌患者预后具有重要意义。展开更多
文摘BACKGROUND The prognostic assessment of patients after surgical resection of gastric cancer(GC)patients is critical.However,the role of the circadian clock gene NPAS2 expression in GC remains unknown.AIM To explore the relationship between NPAS2 and the survival prognosis of GC patients and clarify its role in evaluating GC prognosis.METHODS The tumor tissues and clinical data of 101 patients with GC were collected retrospectively.Immunohistochemical staining(IHC)was used to detect the expression of NPAS2 protein in GC and adjacent tissues.Univariate and multivariate Cox regression analysis was used to determine the independent prognostic factors of GC,and a nomogram prediction model was established.The receiver operating characteristic(ROC)curve,the ROC area under the curve,the calibration curve,and C-index were used to evaluate the predictive effectiveness of the model.Kaplan Meier analysis was used to compare the risk stratification of subgroups according to the median score in the nomogram model of each patient.RESULTS Microarray IHC analysis showed that the positive rate of NPAS2 protein expression in GC tissues was 65.35%,which was significantly higher than 30.69%in adjacent tissues.The high expression of NPAS2 was correlated with tumor-node-metastasis(TNM)stage(P<0.05),pN stage(P<0.05),metastasis(P<0.05),venous invasion(P<0.05),lymphatic invasion(P<0.05),and lymph node positive(P<0.05)of GC.Kaplan Meier survival analysis showed that the 3-year overall survival(OS)of patients with high NPAS2 expression was significantly shortened(P<0.0001).Univariate and multivariate COX regression analysis showed that TNM stage(P=0.009),metastasis(P=0.009),and NPAS2 expression(P=0.020)were independent prognostic factors of OS in GC patients for 3 years.The nomogram prediction model based on independent prognostic factors has a C-Index of 0.740(95%CI:0.713-0.767).Furthermore,subgroup analysis showed that the 3-year OS time of the high-risk group was significantly lower than that of the low-risk group(P<0.0001).CONCLUSION NPAS2 is highly expressed in GC tissues and is closely related to worse OS in patients.Therefore,the evaluation of NPAS2 expression may be a potential marker for GC prognosis evaluation.Notably,the nomogram model based on NPAS2 can improve the accuracy of GC prognosis prediction and assist clinicians in postoperative patient management and decision-making.
文摘目的检测生物节律基因NPAS2在结直肠癌组织及相应癌旁组织中的表达情况;明确NPAS2的表达与患者临床病理学参数之间的相关性,分析NPAS2表达与结直肠癌患者5年生存率之间的相关性,明确其在判断结直肠癌患者预后中的作用。方法采用RT-q PCR检测NPAS2 m RNA在40例结直肠癌及癌旁新鲜组织中的表达;采用免疫组织化学SABC法检测NPAS2蛋白在120例肿瘤及癌旁石蜡标本中的表达;利用其中78例随访患者资料,K-M生存法分析NPAS2蛋白表达水平与患者生存率之间的相关性。结果 RT-q PCR结果提示,结直肠癌中NPAS2 m RNA表达显著低于相对应的癌旁组织(P<0.01);免疫组织化学结果提示,NPAS2蛋白表达在结直肠癌组织中的阳性率为19.2%,显著低于癌旁中的62.5%(P<0.05)。NPAS2的低表达与结直肠癌的肿瘤大小、淋巴结转移及TNM分期密切相关(P<0.05),而与患者年龄、性别、分化、浸润深度及远处转移无显著相关性(P>0.05)。K-M生存分析结果表明,NPAS2高表达组患者5年生存率显著高于低表达组,差异具有统计学意义(P<0.01)。结论NPAS2在结直肠癌组织中低表达,且与肿瘤部分恶性生物学行为及患者5年生存率密切相关,提示检测NPAS2的表达对预测结直肠癌患者预后具有重要意义。
