BACKGROUND:Hepatic ischemia-reperfusion injury is a common phenomenon in hepatic surgical procedures and can result in further severe damage.This study aimed to investigate the protective effects of glutamine precondi...BACKGROUND:Hepatic ischemia-reperfusion injury is a common phenomenon in hepatic surgical procedures and can result in further severe damage.This study aimed to investigate the protective effects of glutamine preconditioning on hepatic ischemia-reperfusion injury in rats and its dose-dependency. METHODS:Thirty-two healthy male Wistar rats were randomly divided into four groups(n=8 per group).One group received 0.9%NaCl(control)and the other three received glutamine(Gln groups)4 hours before ischemia.The Gln groups were named GL,GM,and GH according to the glutamine dose.The liver was subjected to 1 hour of ischemia and 2 hours of reperfusion. Two hours later,the levels of alanine aminotransferase(ALT), intracellular free calcium(Ca 2+ ),and activity of Na + /K + adenosine triphosphatase(ATPase)and superoxide dismutase (SOD)were assessed,and liver tissue sections were examined under a microscope. RESULTS:The Gln and control groups differed in the concentration of intracellular free calcium(P<0.05),and the activity of Na + /K + ATPase and SOD in the Gln groups was higher than in the control group(P<0.05).The ALT level was lower in the GM and GH groups than in the control group(P<0.05).The levels of Na + /K + ATPase and SOD rose gradually with increasing glutamine dose(P<0.05),and the concentration of Ca 2+ declined gradually with increasing glutamine dose(P<0.05).The degree of hepatocyte injury was milder in the Gln groups than in the control group. CONCLUSIONS:Glutamine preconditioning protected effectively against hepatic ischemia-reperfusion injury.These protective effects were related to the dose of glutamine and due to the reduction of intracellular calcium overload and the improvements in the activity of Na + /K + ATPase and SOD.展开更多
文摘BACKGROUND:Hepatic ischemia-reperfusion injury is a common phenomenon in hepatic surgical procedures and can result in further severe damage.This study aimed to investigate the protective effects of glutamine preconditioning on hepatic ischemia-reperfusion injury in rats and its dose-dependency. METHODS:Thirty-two healthy male Wistar rats were randomly divided into four groups(n=8 per group).One group received 0.9%NaCl(control)and the other three received glutamine(Gln groups)4 hours before ischemia.The Gln groups were named GL,GM,and GH according to the glutamine dose.The liver was subjected to 1 hour of ischemia and 2 hours of reperfusion. Two hours later,the levels of alanine aminotransferase(ALT), intracellular free calcium(Ca 2+ ),and activity of Na + /K + adenosine triphosphatase(ATPase)and superoxide dismutase (SOD)were assessed,and liver tissue sections were examined under a microscope. RESULTS:The Gln and control groups differed in the concentration of intracellular free calcium(P<0.05),and the activity of Na + /K + ATPase and SOD in the Gln groups was higher than in the control group(P<0.05).The ALT level was lower in the GM and GH groups than in the control group(P<0.05).The levels of Na + /K + ATPase and SOD rose gradually with increasing glutamine dose(P<0.05),and the concentration of Ca 2+ declined gradually with increasing glutamine dose(P<0.05).The degree of hepatocyte injury was milder in the Gln groups than in the control group. CONCLUSIONS:Glutamine preconditioning protected effectively against hepatic ischemia-reperfusion injury.These protective effects were related to the dose of glutamine and due to the reduction of intracellular calcium overload and the improvements in the activity of Na + /K + ATPase and SOD.
