低盐水体Na^(+)/K^(+)对凡纳滨对虾生长、体成分与肝胰腺、鳃组织结构的影响

缺钾在内陆低盐度盐碱水中经常发生,为探究低盐水体中不同钾缺乏程度对凡纳滨对虾生长、存活、体成分以及鳃和肝胰腺组织结构的影响,实验参照海水离子组成配置盐度为2的低盐度水体,在Na^(+)/K^(+)(mg/mg)比值为27、47、67、87、107 (分别记为A、B、C、D、E组)的条件下,对体重为(1.04±0.23) g的凡纳滨对虾幼虾开展了为期60 d的养殖实验。结果显示,E组对虾的存活率为44.64%±20.95%,显著低于A、B、D三组;E组体重为(4.86±0.66) g,显著低于其他4组;A~D组之间的湿重、增重率、特定生长率差异不显著,但均大于E组且差异显著;在饲料系数上,E组最高并与A、D组差异显著。在体成分上,各组全虾钾含量、灰分含量差异不显著,E组的水分含量高于其他组,并与A、B组差异显著;E组粗蛋白含量最低,且与B组差异显著。E组对虾鳃组织角质层明显受损,红细胞数量减少,空泡增多,肝胰腺B细胞及其内部转运泡体积增大,肝小管结构受损。研究表明,低盐条件下严重缺钾引起了对虾鳃和肝胰腺损伤,降低了对虾的存活率和生长率;水体缺钾在前期即可对对虾的生长产生明显影响,而对存活的影响随养殖时间的增加而增大。实验结果有助于为内陆低盐度盐碱水养殖凡纳滨对虾提供理论依据。

枯草芽孢杆菌YvdSR蛋白转运Na^(+)关键残基的研究

对枯草芽孢杆菌(Bacillus subtilis strain 168)双组分基因YvdSR表达的双组分蛋白YvdSR进行功能鉴定,得出YvdSR具有转运Na^(+)/Li^(+)功能,为探究YvdSR离子转运机制,分别对两种组分作同源序列比对,从每种组分中筛选出完全保守的酸性氨基酸残基并进行氨基酸残基定点突变。结果表明,YvdS和YvdR共有的E13在同源物中完全保守且突变为丙氨酸后均失去Na^(+)(Li^(+))/H^(+)逆向转运活性,说明两种组分的E13均是蛋白质行使功能不可或缺的,YvdS的E13A经回复突变后可恢复高水平Na^(+)(Li^(+))/H^(+)逆向转运活性,YvdR的E13A经回复突变后仍失去Na^(+)(Li^(+))/H^(+)逆向转运活性,表明YvdS和YvdR在Na^(+)(Li^(+))/H^(+)逆向转运中行使不同功能。

Na^(+)/K^(+)-ATPase:ion pump,signal transducer,or cytoprotective protein,and novel biological functions

Na^(+)/K^(+)-ATPase is a transmembrane protein that has important roles in the maintenance of electrochemical gradients across cell membranes by transporting three Na^(+)out of and two K^(+)into cells.Additionally,Na^(+)/K^(+)-ATPase participates in Ca^(2+)-signaling transduction and neurotransmitter release by coordinating the ion concentration gradient across the cell membrane.Na^(+)/K^(+)-ATPase works synergistically with multiple ion channels in the cell membrane to form a dynamic network of ion homeostatic regulation and affects cellular communication by regulating chemical signals and the ion balance among different types of cells.Therefo re,it is not surprising that Na^(+)/K^(+)-ATPase dysfunction has emerged as a risk factor for a variety of neurological diseases.However,published studies have so far only elucidated the important roles of Na^(+)/K^(+)-ATPase dysfunction in disease development,and we are lacking detailed mechanisms to clarify how Na^(+)/K^(+)-ATPase affects cell function.Our recent studies revealed that membrane loss of Na^(+)/K^(+)-ATPase is a key mechanism in many neurological disorders,particularly stroke and Parkinson's disease.Stabilization of plasma membrane Na^(+)/K^(+)-ATPase with an antibody is a novel strategy to treat these diseases.For this reason,Na^(+)/K^(+)-ATPase acts not only as a simple ion pump but also as a sensor/regulator or cytoprotective protein,participating in signal transduction such as neuronal autophagy and apoptosis,and glial cell migration.Thus,the present review attempts to summarize the novel biological functions of Na^(+)/K^(+)-ATPase and Na^(+)/K^(+)-ATPase-related pathogenesis.The potential for novel strategies to treat Na^(+)/K^(+)-ATPase-related brain diseases will also be discussed.

新型Na^(+)/H^(+)逆向转运蛋白UPF0118的Na^(+)结合鉴定

UPF0118蛋白是一种新型Na^(+)(Li^(+))/H^(+)逆向转运蛋白,属于AI-2E超家族,目前已被划分至Na^(+)/H^(+)逆向转运蛋白亚家族,但无直接证据表明该蛋白可与Na+产生相互作用。为鉴定UPF0118蛋白是否具有Na^(+)结合功能,利用PCR技术扩增upf0118基因编码序列,通过Eco RⅠ和PstⅠ酶切位点构建至原核表达载体p ETDuet-1,转化至E. coli BL21*(DE3),对诱导后大量表达的蛋白进行Ni^(2+)亲和层析和凝胶过滤层析,利用等温滴定量热技术对纯化蛋白进行滴定。结果表明,在pH 5.5条件下,Na^(+)滴入导致蛋白溶液产生明显放热现象。在pH 5.5条件下该蛋白具有Na^(+)结合功能,证明该蛋白Na+结合功能为其功能单元与分子机制的研究奠定基础。

On the functions of astrocyte-mediated neuronal slow inward currents

Slow inward currents are known as neuronal excitatory currents mediated by glutamate release and activation of neuronal extra synaptic N-met hyl-D-aspartate receptors with the contribution of astrocytes.These events are significantly slower than the excitatory postsynaptic currents.Parameters of slow inward currents are determined by seve ral factors including the mechanisms of astrocytic activation and glutamate release,as well as the diffusion pathways from the release site towards the extra synaptic recepto rs.Astrocytes are stimulated by neuronal network activity,which in turn excite neurons,forming an astrocyte-neuron feedback loop.Mostly as a consequence of brain edema,astrocytic swelling can also induce slow inward currents under pathological conditions.There is a growing body of evidence on the roles of slow inward currents on a single neuron or local network level.These events often occur in synchro ny on neurons located in the same astrocytic domain.Besides synchronization of neuronal excitability,slow inward currents also set synaptic strength via eliciting timing-dependent synaptic plasticity.In addition,slow inward currents are also subject to non-synaptic plasticity triggered by long-la sting stimulation of the excitatory inputs.Of note,there might be important regionspecific differences in the roles and actions triggering slow inward currents.In greater networks,the pathophysiological roles of slow inward currents can be better understood than physiological ones.Slow inward currents are identified in the pathophysiological background of autism,as slow inward currents drive early hypersynchrony of the neural networks.Slow inward currents are significant contributors to paroxysmal depolarizational shifts/interictal spikes.These events are related to epilepsy,but also found in Alzheimer's disease,Parkinson's disease,and stroke,leading to the decline of cognitive functions.Events with features overlapping with slow inward currents(excitatory,N-methyl-Daspartate-receptor mediated currents with astrocytic contribution) as ischemic currents and spreading depolarization also have a well-known pathophysiological role in worsening consequences of stroke,traumatic brain injury,or epilepsy.One might assume that slow inward currents occurring with low frequency under physiological conditions might contribute to synaptic plasticity and memory formation.However,to state this,more experimental evidence from greater neuronal networks or the level of the individual is needed.In this review,I aimed to summarize findings on slow inward currents and to speculate on the potential functions of it.

Lift-Off Effect of Koch and Circular Differential Pickup Eddy Current Probes

A flexible or planar eddy current probe with a differential structure can suppress the lift-off noise during the inspection of defects.However,the extent of the lift-off effect on differential probes,including different coil structures,varies.In this study,two planar eddy current probes with differential pickup structures and the same size,Koch and circular probes,were used to compare lift-off effects.The eddy current distributions of the probes perturbed by 0°and 90°cracks were obtained by finite element analysis.The analysis results show that the 90°crack can impede the eddy current induced by the Koch probe even further at relatively low lift-off distance.The peak-to-peak values of the signal output from the two probes were compared at different lift-off distances using finite element analysis and experimental methods.In addition,the effects of different frequencies on the lift-off were studied experimentally.The results show that the signal peak-to-peak value of the Koch probe for the inspection of cracks in 90°orientation is larger than that of the circular probe when the lift-off distance is smaller than 1.2 mm.In addition,the influence of the lift-off distance on the peak-to-peak signal value of the two probes was studied via normalization.This indicates that the influence becomes more evident with an increase in excitation frequency.This research discloses the lift-off effect of differential planar eddy current probes with different coil shapes and proves the detection merit of the Koch probe for 90°cracks at low lift-off distances.

Evidence of guide field magnetic reconnection in flapping current sheets

Based on current sheet flapping motion on 27 August 2018 in the dusk flank magnetotail,as recorded by instruments aboard Magnetospheric Multiscale(MMS)spacecraft,we present the first study of guide field reconnection observed in the flux rope embedded in kink-like flapping current sheets near the dusk-side flank of the magnetotail.Unlike more common magnetotail reconnections,which are symmetric,these asymmetric small-scale(λ_(i)~650 km)reconnections were found in the highly twisted current sheet when the direction normal to the sheet changes from the Z direction into the Y direction.The unique feature of this unusual reconnection is that the reconnection jets are along the Z direction-different from outflow in the X direction,which is the more usual situation.This vertical reconnection jet is parallel or antiparallel to the up-and-down motion of the tail’s current sheet.The normalized reconnection rate R is estimated to be~0.1.Our results indicate that such asymmetric reconnections can significantly enlarge current sheet flapping,with large oscillation amplitudes.This letter presents direct evidence of guide field reconnection in a highly twisted current sheet,characterized by enlarged current sheet flapping as a consequence of the reconnection outflow.

Double-ring high-frequency common-mode switching oscillation current sensor for inverter-fed machine winding insulation monitoring

Insulation failure significantly contributes to the unpredictable shutdown of power equipment.Compared to the partial discharge and high-frequency(HF)injection methods,the HF common-mode(CM)leakage current method offers a non-intrusive and highly sensitive alternative.However,the detection of HF CM currents is susceptible to interference from differential-mode(DM)currents,which exhibit high-amplitude and multifrequency components during normal operation.To address this challenge,this paper proposes a double-ring current sensor based on the principle of magnetic shielding for inverter-fed machine winding insulation monitoring.The inner ring harnesses the magnetic aggregation effect to isolate the DM current magnetic field,whereas the outer ring serves as the magnetic core of the Rogowski current sensor,enabling HF CM current monitoring.First,the magnetic field distributions of the CM and DM currents were analyzed.Then,a correlation between the sensor parameters and signal-to-noise ratio of the target HF CM current was established.Finally,an experimental study was conducted on a 3-kW PMSM for verification.The results indicate that the proposed double-ring HF CM sensor can effectively mitigate DM current interference.Compared to a single-ring sensor,a reduction of approximately 40%in the DM component was achieved,which significantly enhanced the precision of online insulation monitoring.

Research and exploration of direct current power supply and distribution systems for Antarctic research stations

The power supply and distribution systems for Antarctic research stations have special characteristics.In light of a worldwide trend toward a gradual increase in the application of renewable energy,an analysis was performed to assess the feasibility of achieving a direct current power supply and distribution at Antarctic research stations by comparing the characteristics of direct current and alternating current electricity.Research was also performed on the status quo and future trends in direct current power supply and distribution systems in Antarctica research stations in combination with case studies.

Numerical analysis for the free-boundary current reversal equilibrium in the AC plasma current operation in a tokamak

In recent decades, tokamak discharges with zero total toroidal current have been reported in tokamak experiments, and this is one of the key problems in alternating current(AC) operations.An efficient free-boundary equilibrium code is developed to investigate such advanced tokamak discharges with current reversal equilibrium configuration. The calculation results show that the reversal current equilibrium can maintain finite pressure and also has considerable effects on the position of the X-point and the magnetic separatrix shape, and hence also on the position of the strike point on the divertor plates, which is extremely useful for magnetic design, MHD stability analysis, and experimental data analysis etc. for the AC plasma current operation on tokamaks.

Analysis of Sea Surface Temperature Cooling in Typhoon Events Passing the Kuroshio Current

The aim of this study is to investigate the sea surface temperature(SST) cooling as typhoons pass the Kuroshio Current.A numerical circulation model,denoted as the Stony Brook Parallel Ocean Model(sbPOM),was used to simulate the SST,which includes four wave-induced effect terms(i.e.,radiation stress,nonbreaking waves,Stokes drift,and breaking waves) simulated using the third-generation wave model,called WAVEWATCH-Ⅲ(WW3).The significant wave height(SWH) measurements from the Jason-2 altimeter were used to validate the WW3-simulated results,yielding a root mean square error(RMSE) of less than 0.50 m and a correlation coefficient(COR) of approximately 0.93.The water temperature measured from the Advanced Research and Global Observation Satellite was applied to validate the model simulation.Accordingly,the RMSE of the SST is 0.92℃ with a COR of approximately 0.99.As revealed in the sbPOM-simulated SST fields,a reduction in the SST at the Kuroshio Current region was observed as a typhoon passed,although the water temperature of the Kuroshio Current is relatively high.The variation of the SST is consistent with that of the current,whereas the maximum SST lagged behind the occurrence of the peak SWH.Moreover,the Stokes drift plays an important role in the SST cooling after analyzing four wave-induced terms in the background of the Kuroshio Current.The sensitivity experiment also showed that the accuracy of the water temperature was significantly reduced when including breaking waves,which play a negative role in the inside part of the ocean.The variation in the mean mixing layer depth(MLD) showed that a typhoon could enhance the mean MLD in the Kuroshio Current area in September and October,whereas a typhoon has little influence on the mean MLD in the Kuroshio Current area in May.Moreover,the mean MLD rapidly decreased with the weakening of the strong wind force and wave-induced effects when a typhoon crossed the Kuroshio Current.

Alternating current heating techniques for lithium-ion batteries in electric vehicles:Recent advances and perspectives

The significant decrease in battery performance at low temperatures is one of the critical challenges that electric vehicles(EVs)face,thereby affecting the penetration rate in cold regions.Alternating current(AC)heating has attracted widespread attention due to its low energy consumption and uniform heating advantages.This paper introduces the recent advances in AC heating from the perspective of practical EV applications.First,the performance degradation of EVs in low-temperature environments is introduced briefly.The concept of AC heating and its research methods are provided.Then,the effects of various AC heating methods on battery heating performance are reviewed.Based on existing studies,the main factors that affect AC heating performance are analyzed.Moreover,various heating circuits based on EVs are categorized,and their cost,size,complexity,efficiency,reliability,and heating rate are elaborated and compared.The evolution of AC heaters is presented,and the heaters used in brand vehicles are sorted out.Finally,the perspectives and challenges of AC heating are discussed.This paper can guide the selection of heater implementation methods and the optimization of heating effects for future EV applications.

Influence of Current Density on the Photocatalytic Activity of Nd:TiO_(2) Coatings

The Nd:TiO_(2 )PEO coatings were formed in a phosphate-based electrolyte with the addition of Nd_(2)O_(3 )under the current density of 150,200,250 and 300 m A/cm^(2).SEM results showed that the micropores decreased on quantity and increased on scale with the increasing current density.AFM results revealed that the roughness of the coatings increased with the increasing current density.Phase and composition analysis showed that the Nd:TiO_(2) coatings were mainly composed of anatase and rutile phase.And the anatase phase content has reached the maximum value at the current density of 250 m A/cm^(2).XPS results indicated that Ti2p spin-orbit components of the Nd:TiO_(2) coatings are shifted towards higher binding energy,compared with the pure TiO_(2) coating,suggesting that some of the Nd^(3+)ions are combined with TiO_(2) lattice and led to dislocation.Photocatalytic test showed that the photocatalytic activity of Nd:TiO_(2) coatings varied in the same pattern with the anatase content variation in Nd:TiO_(2) coatings.The photocatalytic experiment results show that the photocatalytic activity of Nd:TiO_(2) coatings can be greatly enhanced with moderate amount of Nd^(3+).However,excessive amount of Nd^(3+)does not have an effective impact on the photoctalytic activity improvement.

Recent advances in design of hydrogen evolution reaction electrocatalysts at high current density:A review

The electrolysis of water powered by renewable energy sources offers a promising method of"green hydrogen"production,which is considered to be at the heart of future carbon-neutral energy systems.In the past decades,researchers have reported a number of hydrogen evolution reaction(HER)electrocatalysts with activity comparable to that of commercial Pt/C,but most of them are tested within a small current density range,typically no more than 500 mA cm^(-2).To realize the industrial application of hydrogen production from water electrolysis,it is essential to develop high-efficiency HER electrocatalysts at high current density(HCD≥500 mA cm^(-2)).Nevertheless,it remains challenging and significant to rational design HCD electrocatalysts for HER.In this paper,the design strategy of HCD electrocatalysts is discussed,and some HCD electrocatalysts for HER are reviewed in seven categories(alloy,metal oxide,metal hydroxide,metal sulfide/selenide,metal nitride,metal phosphide and other derived electrocatalysts).At the end of this article,we also pro-pose some viewpoints and prospects for the future development and research directions of HCD electrocatalysts for HER.

Plasma current tomography for HL-2A based on Bayesian inference

An accurate plasma current profile has irreplaceable value for the steady-state operation of the plasma.In this study,plasma current tomography based on Bayesian inference is applied to an HL-2A device and used to reconstruct the plasma current profile.Two different Bayesian probability priors are tried,namely the Conditional Auto Regressive(CAR)prior and the Advanced Squared Exponential(ASE)kernel prior.Compared to the CAR prior,the ASE kernel prior adopts nonstationary hyperparameters and introduces the current profile of the reference discharge into the hyperparameters,which can make the shape of the current profile more flexible in space.The results indicate that the ASE prior couples more information,reduces the probability of unreasonable solutions,and achieves higher reconstruction accuracy.

“Flow” Transcranial Direct Current Stimulation (tDCS) for Depression Treatment in a Primary Healthcare General Practice—An Open-Label Cohort Study Measuring Montgomery-Åsberg Depression Rating Scale (MADRS-S) Outcomes

Background: Flow FL-100 is a transcranial direct current stimulation (tDCS) device self-administered by a patient at home in combination with a software application delivered wellbeing behaviour therapy training. tDCS has evidence of effectiveness in treating symptoms of depression. Purpose/Aim: This post marketing study evaluated the effect of Flow on depression for primary care general practice patients with depression symptoms. Methods: Open-label patient cohort design with no control group. Inclusion criteria were aged 18 years or over and reporting depression symptoms. Participants self-administered five 30 minute tDCS sessions per week for the first three weeks, and then 3 sessions per week following this. Three, six and ten week assessment with participant self-report measure: Montgomery- Åsberg Depression Rating Scale (MADRS-S). Results: MADRS-S remission rates were between 29% - 30% at three weeks, 33% - 34% at six-weeks and 50% at 10-weeks treatment. There was a significant improvement in MADRS-S with large effect sizes at all time points. Conclusions: Flow tDCS can be delivered through a primary healthcare general practice service and patients will choose to use. Flow tDCS provides an effective depression treatment in addition and as an alternative to antidepressants and psychotherapy. tDCS has evidence as an effective depression treatment, and the widespread availability of tDCS in primary care general practice should be considered.

Fault Estimation for a Class of Markov Jump Piecewise-Affine Systems: Current Feedback Based Iterative Learning Approach

In this paper, the issues of stochastic stability analysis and fault estimation are investigated for a class of continuoustime Markov jump piecewise-affine(PWA) systems against actuator and sensor faults. Firstly, a novel mode-dependent PWA iterative learning observer with current feedback is designed to estimate the system states and faults, simultaneously, which contains both the previous iteration information and the current feedback mechanism. The auxiliary feedback channel optimizes the response speed of the observer, therefore the estimation error would converge to zero rapidly. Then, sufficient conditions for stochastic stability with guaranteed performance are demonstrated for the estimation error system, and the equivalence relations between the system information and the estimated information can be established via iterative accumulating representation.Finally, two illustrative examples containing a class of tunnel diode circuit systems are presented to fully demonstrate the effectiveness and superiority of the proposed iterative learning observer with current feedback.

Revisiting aluminum current collector in lithium-ion batteries:Corrosion and countermeasures

With the large-scale service of lithium-ion batteries(LIBs),their failures have attracted significant attentions.While the decay of active materials is the primary cause for LIB failures,the degradation of auxiliary materials,such as current collector corrosion,should not be disregarded.Therefore,it is necessary to conduct a comprehensive review in this field.In this review,from the perspectives of electrochemistry and materials,we systematically summarize the corrosion behavior of aluminum cathode current collector and propose corresponding countermeasures.Firstly,the corrosion type is clarified based on the properties of passivation layers in different organic electrolyte components.Furthermore,a thoroughgoing analysis is presented to examine the impact of various factors on aluminum corrosion,including lithium salts,organic solvents,water impurities,and operating conditions.Subsequently,strategies for electrolyte and protection layer employed to suppress corrosion are discussed in detail.Lastly and most importantly,we provide insights and recommendations to prevent corrosion of current collectors,facilitate the development of advanced current collectors and the implementation of next-generation high-voltage stable LIBs.

Pressure stimulated current in progressive failure process of combined coal-rock under uniaxial compression:Response and mechanism

Effective monitoring of the structural health of combined coal-rock under complex geological conditions by pressure stimulated currents(PSCs)has great potential for the understanding of dynamic disasters in underground engineering.To reveal the effect of this way,the uniaxial compression experiments with PSC monitoring were conducted on three types of coal-rock combination samples with different strength combinations.The mechanism explanation of PSCs are investigated by resistivity test,atomic force microscopy(AFM)and computed tomography(CT)methods,and a PSC flow model based on progressive failure process is proposed.The influence of strength combinations on PSCs in the progressive failure process are emphasized.The results show the PSC responses between rock part,coal part and the two components are different,which are affected by multi-scale fracture characteristics and electrical properties.As the rock strength decreases,the progressive failure process changes obviously with the influence range of interface constraint effect decreasing,resulting in the different responses of PSC strength and direction in different parts to fracture behaviors.The PSC flow model is initially validated by the relationship between the accumulated charges of different parts.The results are expected to provide a new reference and method for mining design and roadway quality assessment.

Microstructure and mechanical properties of Co-28Cr-6Mo-0.22C investment castings by current solution treatment

This study examined the impact of current solution treatment on the microstructure and mechanical properties of the Co-28Cr-6Mo-0.22C alloy investment castings.The findings reveal that the current solution treatment significantly promotes the dissolution of carbides at a lower temperature.The optimal conditions for solution treatment are determined as a solution temperature of 1,125°C and a holding time of 5.0 min.Under these parameters,the size and volume fraction of precipitated phases in the investment castings are measured as6.2μm and 1.1vol.%.The yield strength,ultimate tensile strength,and total elongation of the Co-28Cr-6Mo-0.22C investment castings are 535 MPa,760 MPa,and 12.6%,respectively.These values exceed those obtained with the conventional solution treatment at 1,200°C for 4.0 h.The findings suggest a phase transformation of M_(23)C_(6)→σ+C following the current solution treatment at 1,125°C for 5.0 min.In comparison,the traditional solution treatment at 1,200°C for 4.0 h leads to the formation of M_(23)C_(6)and M_(6)C carbides.It is noteworthy that the non-thermal effect of the current during the solution treatment modifies the free energy of both the matrix and precipitation phase.This modification lowers the phase transition temperature of the M_(23)C_(6)→σ+C reaction,thereby facilitating the dissolution of carbides.As a result,the current solution treatment approach achieves carbide dissolution at a lower temperature and within a significantly shorter time when compared to the traditional solution treatment methods.