Aim: To explore the possible mechanisms of male infertility caused by antisperm antibody (AsAb). Methods: Thesoluble interleukin-2 receptor (sIL-2R) level in serum was analyzed by ELISA and Na^+ -K^+ -exchanging ATPas...Aim: To explore the possible mechanisms of male infertility caused by antisperm antibody (AsAb). Methods: Thesoluble interleukin-2 receptor (sIL-2R) level in serum was analyzed by ELISA and Na^+ -K^+ -exchanging ATPase activi-ty in semen by phosphorus (Pi) assay. Results: The slL-2R level in serum was significantly higher and the Na^+ -K^+ -exchanging ATPase activity in semen significantly lower in AsAb positive infertile men when compared with thecontrols. Conclusion: The AsAb titer varies with the slL-2R level in serum. A decrease in Na^+ -K^+ -exchangingATPase activity in semen may play a role in male infertility caused by AsAb.展开更多
The increase in bovine dairy consumption has been correlated with the development of many inflammatory diseases.Emerging evidence points to milk exosomes as potential causative agents.The cargo of exosome consists of ...The increase in bovine dairy consumption has been correlated with the development of many inflammatory diseases.Emerging evidence points to milk exosomes as potential causative agents.The cargo of exosome consists of cytokines that are elevated in inflammatory bowel disease and modulate the activity of the colonic Na^(+)/K^(+)ATPase.Whether a cause effect relationship exists between the exosomes and the Na^(+)/K^(+)ATPase is a question that has not been addressed before and constitutes the aim of this work,using Caco-2 cells as a model.Exosomes were extracted from skim milk by a series of ultra-centrifugations and filtrations.The activity of the pump was assayed by measuring the amount of inorganic phosphate liberated in presence and absence of ouabain.The exosomes increased dose and time-dependently the activity of the ATPase with a maximal effect observed at 1 h.This increase was mediated via PGE2 and still appeared in presence of a blocker of EP1 and EP2,was enhanced further when EP4 was blocked,and changed to an inhibition in presence of an EP3 antagonist,suggesting an involvement of both EP3 and EP4.The activation of the Na^(+)/K^(+)ATPase was significantly enhanced or reduced when cells were treated respectively with an inhibitor or an activator of PKA.Since EP4 and EP3 act via Gs and Gi respectively,they exert opposite effects on PKA and consequently on the ATPase.The stimulatory effect of EP3 on the ATPase predominates over that of EP4 resulting in a net activation of the pump.展开更多
The plant Begonia roxburghii,widely distributed in North East India,is regularly used as a vegetable and food item in these areas.The plant’s roots are traditionally used in treating digestive disorders,diarrhoea,dys...The plant Begonia roxburghii,widely distributed in North East India,is regularly used as a vegetable and food item in these areas.The plant’s roots are traditionally used in treating digestive disorders,diarrhoea,dysentery and various stomach ailments.Therefore,the main objective included scientific justification of the traditional assertions about the edible roots and its marker flavonoids from the plant in the treatment of diarrhoea,followed by deriving its mechanism.HPLC was used to standardize the ethanolic extract of B.roxburghii(EBR)with respective markers luteolin and rutin.The study revealed the similar antibacterial potential of both the markers(luteolin and rutin);however,normal faecal excretion showed the highest antisecretory potential of EBR followed by rutin.EBR at 300 mg/kg,p.o.demonstrated the highest%protection from diarrhoea in both nonpathogenic(castor oil-induced)and pathogenic(enteropathogenic E.coli-induced)diarrhoea rat models,which also restored the altered biochemical parameters,ion concentration and cytokine levels.EBR and rutin also justified their protective nature through histopathology showing less broadening of villus and intact epithelia compared to diarrhoea control rat colons.The in-silico studies validated the contribution of rutin as a major biomarker which enhanced Na+/K+-ATPase activity and stabilized the C-terminal 282-residue fragment of EPEC intimin and was also supported by findings of network pharmacology observations.The present investigation justified the traditional claim of the edible roots of B.roxburghii in treating diarrhoea,where its marker rutin played a significant role in reactivating Na^(+)/K^(+)-ATPase,restored electrolyte balance,and also stabilized the EPEC C-terminal.展开更多
文摘Aim: To explore the possible mechanisms of male infertility caused by antisperm antibody (AsAb). Methods: Thesoluble interleukin-2 receptor (sIL-2R) level in serum was analyzed by ELISA and Na^+ -K^+ -exchanging ATPase activi-ty in semen by phosphorus (Pi) assay. Results: The slL-2R level in serum was significantly higher and the Na^+ -K^+ -exchanging ATPase activity in semen significantly lower in AsAb positive infertile men when compared with thecontrols. Conclusion: The AsAb titer varies with the slL-2R level in serum. A decrease in Na^+ -K^+ -exchangingATPase activity in semen may play a role in male infertility caused by AsAb.
基金funded by a grant from the American University of Beirut Research Board.
文摘The increase in bovine dairy consumption has been correlated with the development of many inflammatory diseases.Emerging evidence points to milk exosomes as potential causative agents.The cargo of exosome consists of cytokines that are elevated in inflammatory bowel disease and modulate the activity of the colonic Na^(+)/K^(+)ATPase.Whether a cause effect relationship exists between the exosomes and the Na^(+)/K^(+)ATPase is a question that has not been addressed before and constitutes the aim of this work,using Caco-2 cells as a model.Exosomes were extracted from skim milk by a series of ultra-centrifugations and filtrations.The activity of the pump was assayed by measuring the amount of inorganic phosphate liberated in presence and absence of ouabain.The exosomes increased dose and time-dependently the activity of the ATPase with a maximal effect observed at 1 h.This increase was mediated via PGE2 and still appeared in presence of a blocker of EP1 and EP2,was enhanced further when EP4 was blocked,and changed to an inhibition in presence of an EP3 antagonist,suggesting an involvement of both EP3 and EP4.The activation of the Na^(+)/K^(+)ATPase was significantly enhanced or reduced when cells were treated respectively with an inhibitor or an activator of PKA.Since EP4 and EP3 act via Gs and Gi respectively,they exert opposite effects on PKA and consequently on the ATPase.The stimulatory effect of EP3 on the ATPase predominates over that of EP4 resulting in a net activation of the pump.
基金Senior Research Fellowship(Letter No.45/18/2022/TRM/BMS)awarded to Mrs.Rupali S.Prasad by the Indian Council of Medical Research,Ministry of Health&Family Welfare,Government of India.
文摘The plant Begonia roxburghii,widely distributed in North East India,is regularly used as a vegetable and food item in these areas.The plant’s roots are traditionally used in treating digestive disorders,diarrhoea,dysentery and various stomach ailments.Therefore,the main objective included scientific justification of the traditional assertions about the edible roots and its marker flavonoids from the plant in the treatment of diarrhoea,followed by deriving its mechanism.HPLC was used to standardize the ethanolic extract of B.roxburghii(EBR)with respective markers luteolin and rutin.The study revealed the similar antibacterial potential of both the markers(luteolin and rutin);however,normal faecal excretion showed the highest antisecretory potential of EBR followed by rutin.EBR at 300 mg/kg,p.o.demonstrated the highest%protection from diarrhoea in both nonpathogenic(castor oil-induced)and pathogenic(enteropathogenic E.coli-induced)diarrhoea rat models,which also restored the altered biochemical parameters,ion concentration and cytokine levels.EBR and rutin also justified their protective nature through histopathology showing less broadening of villus and intact epithelia compared to diarrhoea control rat colons.The in-silico studies validated the contribution of rutin as a major biomarker which enhanced Na+/K+-ATPase activity and stabilized the C-terminal 282-residue fragment of EPEC intimin and was also supported by findings of network pharmacology observations.The present investigation justified the traditional claim of the edible roots of B.roxburghii in treating diarrhoea,where its marker rutin played a significant role in reactivating Na^(+)/K^(+)-ATPase,restored electrolyte balance,and also stabilized the EPEC C-terminal.
