Both pentachlorophenol and 2,3,7,8-tetrachlorodibenzo-p-dioxin （TCDD） had been studied widely because of their probable anti-estrogenic activity. Sodium pentachlorophenol （PCP-Na）, as a industrial product used in ...Both pentachlorophenol and 2,3,7,8-tetrachlorodibenzo-p-dioxin （TCDD） had been studied widely because of their probable anti-estrogenic activity. Sodium pentachlorophenol （PCP-Na）, as a industrial product used in many fields, usually contains a trace of TCDD. The aim of this study was to assess the anti-estrogenic effect of PCP-Na in juvenile goldfish （Gurassius auratus） hepatocyte cultures using vitellogenin （VTG） as the biomarker. The ID50 of PCP-Na was investigated and then a series of concentrations （0.001 0.5 μg/ml） of PCP-Na were evaluated to estimate the anti-estrogenic activity. Results showed that PCP-Na was cytotoxic for hepatocytes even at very low concentration 〈1.21 μg/ml, and it could not induce VTG at any concentrations tested. Since it failed to stimulate VTG production, the possibility of its anti-estrogenic effect was tested, and a well-known anti-estrogenic compound-tamoxifen was used as positive control. PCP-Na caused a reduction in VTG synthesis in juvenile goldfish （Carassius auratus） hepatocytes at concentrations 〉0.1μg/ml when co-exposure with 1μg/ml 17β-estradiol （E2）, making its anti-estrogenic activity approximately as potent as tamoxifen. Our results indicate that PCP-Na can act as negative modulators of estrogenic function in juvenile goldfish （Carassius auratus） hepatocytes.展开更多
Titanium dioxide(TiO 2) samples of different crystal forms were prepared by hydrolysis tetrabutyl titanate in various water to alkoxide ratios and sintering the hydrolysis product at different temperatures. The photo...Titanium dioxide(TiO 2) samples of different crystal forms were prepared by hydrolysis tetrabutyl titanate in various water to alkoxide ratios and sintering the hydrolysis product at different temperatures. The photocatalysts coated on hollow glass beads and loaded with platinum varying from 0.2% to 2.4% by weight.The photocatalytic degradation rate of sodium pentachlorophenolate (PCP-Na) depends on the preparing conditions such as: sintering temperatures, water to alkoxide ratios( R ), platinum content and the size. The proper conditions of preparation photocatalysts are as follows: the ratio of TiO 2 : sodium silicate : hollow glass beads : platinum is 10:5:20:0.15(w/w), R is 100, sintering temperature is 650℃, and the size of hollow glass is 0.5-1 mm. Under these conditions, the ratio between acatase and rutile of the photocatalyst is 2:1, and the photocatalytic activity is high.展开更多
基金The National Natural Science Foundation of China (No. 40332023)
文摘Both pentachlorophenol and 2,3,7,8-tetrachlorodibenzo-p-dioxin （TCDD） had been studied widely because of their probable anti-estrogenic activity. Sodium pentachlorophenol （PCP-Na）, as a industrial product used in many fields, usually contains a trace of TCDD. The aim of this study was to assess the anti-estrogenic effect of PCP-Na in juvenile goldfish （Gurassius auratus） hepatocyte cultures using vitellogenin （VTG） as the biomarker. The ID50 of PCP-Na was investigated and then a series of concentrations （0.001 0.5 μg/ml） of PCP-Na were evaluated to estimate the anti-estrogenic activity. Results showed that PCP-Na was cytotoxic for hepatocytes even at very low concentration 〈1.21 μg/ml, and it could not induce VTG at any concentrations tested. Since it failed to stimulate VTG production, the possibility of its anti-estrogenic effect was tested, and a well-known anti-estrogenic compound-tamoxifen was used as positive control. PCP-Na caused a reduction in VTG synthesis in juvenile goldfish （Carassius auratus） hepatocytes at concentrations 〉0.1μg/ml when co-exposure with 1μg/ml 17β-estradiol （E2）, making its anti-estrogenic activity approximately as potent as tamoxifen. Our results indicate that PCP-Na can act as negative modulators of estrogenic function in juvenile goldfish （Carassius auratus） hepatocytes.
文摘Titanium dioxide(TiO 2) samples of different crystal forms were prepared by hydrolysis tetrabutyl titanate in various water to alkoxide ratios and sintering the hydrolysis product at different temperatures. The photocatalysts coated on hollow glass beads and loaded with platinum varying from 0.2% to 2.4% by weight.The photocatalytic degradation rate of sodium pentachlorophenolate (PCP-Na) depends on the preparing conditions such as: sintering temperatures, water to alkoxide ratios( R ), platinum content and the size. The proper conditions of preparation photocatalysts are as follows: the ratio of TiO 2 : sodium silicate : hollow glass beads : platinum is 10:5:20:0.15(w/w), R is 100, sintering temperature is 650℃, and the size of hollow glass is 0.5-1 mm. Under these conditions, the ratio between acatase and rutile of the photocatalyst is 2:1, and the photocatalytic activity is high.
