非小细胞肺癌中Naked1的表达及意义

目的探讨Naked1(NKD1)蛋白在非小细胞肺癌(non-small cell lung cancer,NSCLC)组织中的表达与预后的关系。方法采用RT-PCR、Western blot法对35例新鲜肺癌组织进行检测;采用免疫组化SP法对100例NSCLC石蜡标本进行检测。结果在79%的新鲜肺癌标本中,NKD1的蛋白水平明显低于相对应的癌旁正常肺组织,而mRNA水平明显高于正常肺组织(P<0.01)。在78%的NSCLC免疫组化标本中,NKD1的表达明显低于正常肺组织(P<0.01)。在肺鳞癌中NKD1的表达低于腺癌,并与分化程度呈正相关(P<0.01),与淋巴结转移(P<0.05)和TNM分期(P<0.01)呈负相关,NKD1低表达者的术后生存时间明显低于正常表达者(P=0.01)。结论在NSCLC中,NKD1 mRNA上调,而蛋白水平下调,其蛋白的下调程度与临床病理因素和不良预后相关。

Pirh2和Naked2在乳腺癌组织中的表达及其临床意义

目的:分析乳腺癌组织中Pirh2和Naked2的表达情况及其临床意义。方法:随机选取在2011年1月—2013年12月期间收治的96例乳腺癌标本作为观察组,同时选取癌旁组织作为对照组,应用免疫组织化学方法对Pirh2和Naked2表达情况进行检测,分析Pirh2和Naked2表达与乳腺癌病理分级的关系及Pirh2和Naked2两者的相关性。结果:Pirh2在乳腺癌组织中高表达,癌旁组织中低表达,Naked2在乳腺癌组织中低表达,癌旁组织中高表达(P<0.05);年龄、肿瘤大小与癌中Pirh2和Naked2的表达无关(P>0.05);肿瘤病理分级和淋巴结转移与Pirh2和Naked2的表达相关(P<0.05);Pirh2和Naked2的表达呈负相关。结论:Pirh2和Naked2对乳腺癌的生物学行为的判断及预后有一定的提示作用。

Analysis of the application and practice of PDCA cycle in management of the naked medicine dispensing——the quality and safety of the drug

Objective:To explore the application of plan–do–check–action(PDCA)cycle in the management of the naked medicine dispensing about the automatic package and to analyze the practice improved in the quality of naked medicine dispensing medicine management.Methods:The PDCA theory was used to analyze the data,to find out the causes of the problem,and to formulate the corresponding countermeasures to intervene.The data of the naked medicine/adverse drug events satisfaction degree of the inpatients in 18 inpatient departments given for the adoption of PDCA cycle in January 2017 were set as the observation group.The number of the naked medicine/adverse drug events satisfaction degree of patients in 18 inpatient departments before adoption of PDCA cycle in December 2016 was set as the control group.The number of the naked medicine/adverse drug events satisfaction degree of patients in 18 inpatient departments before and after adoption of PDCA cycle was observed and analyzed.Results:The number of the naked medicine/adverse drug events after implementing the method of PDCA cycle management rate was significantly lower than before the implementation method of PDCA cycle management,and the difference had statistical significance(P<0.05).The satisfaction degree of the inpatients after implementing the method of PDCA cycle management rate was significantly higher than that before the implementation method of PDCA cycle management,and the difference had statistical significance(P<0.05).Conclusion:Application of PDCA cycle in the management of the naked medicine dispensing about the automatic package can help to reduce the adverse drug events,to improve the safety of drug treatment,and to increase the patients’satisfaction degree.

胃腺癌组织Naked1和MMP-9表达及其相关性研究

目的:观察胃腺癌组织Naked1和MMP-9蛋白表达特征,探讨两者的关系及临床价值。方法:选择97例胃腺癌术后留取的蜡块作为胃癌组,选择62例正常胃黏膜组织作为正常对照组,应用免疫组织化学技术检测两组中Naked1和MMP-9蛋白表达,观察Naked1和MMP-9表达与临床病理特征的相关性。结果:胃癌组Naked1蛋白表达阳性率明显低于正常对照组(P<0.01),MMP-9蛋白表达阳性率明显高于正常对照组(P<0.01)。胃癌组Naked1和MMP-9蛋白表达与肿瘤的临床分期、淋巴结转移、浸润深度、Ki67表达密切相关(P<0.05或P<0.01);Naked1和MMP-9表达具有负相关性(P<0.05),生存分析显示Naked1低表达、MMP-9高表达患者预后差。结论:胃腺癌组织Naked1和MMP-9呈异常表达,两者对病变的进展具有重要作用,术后检测Naked1和MMP-9表达可能对判断预后有一定价值。

The effects of steaming and roasting treatments on lipase activity and nutritional components of “oat rice” (OR): the peeled naked oat (Avena nuda) kernels

Peeled naked oat kernels, named “oat rice” (OR) by Chinese food scientists and processors, are novel oat products in China. This study exam-ined the effects of steaming and roasting treat-ments on the enzyme activities, nutritional con-tents, and flour pasting properties of OR kernels. Results showed that a peeling time of 20 s caused 16.13% β-glucan loss, while a peeling time 25 s caused 34.29% β-glucan loss in the kernels. OR kernels with a 20 s peeling treatment demonstrated significantly higher starch levels and kernel whiteness compared with normal oat kernels (P<0.01). It was also found that normal pressure steaming, autoclaved steaming and infrared roasting treatments could exterminate lipase activities in the OR kernels, and provide the OR kernels with significantly lower final viscosities and setback values than normal kernels (P<0.01).

Effects of Peeling with Flexible Alloy Blade on Naked Oats

This research reported the effect of peeling naked oats with a peeling machine equipped with the flexible alloy blade.Results showed that the flexible alloy blade could achieve the same effect as traditional abrasive rolls. Furthermore, the new peeling method had hardly damage to the oat kernels. The result of scanning electron microscopy indicated the surface of peeled naked oats by the flexible alloy blade is homogeneous. The gap between the flexible alloy blade and the slotted screen could change the particle sizes of the flours obtained, which differed from traditional peeling machines. In addition, peeling for 15 seconds significantly reduced the microbial contaminants. The removal of outer layer decreased the lipase activity. The technological parameters were optimized by orthogonal L_9(3~4) test, the results showed a 30 s peeling time and 2% second-addition of water contributed to the peeling rate. The texture analysis demonstrate that the hardness of cooked groats decreases obviously after peeling treatments.

Assessment of Stress on Serum Estradiol and Cortisol Levels in Female Subordinate Naked Mole Rats Following Isolation from Natal Colony

This study investigated the effects of isolation of subordinate naked mole rats from natal colonies on their reproductive success. Naked mole rat is one of the known mammals that exhibit eusociality. It has been noted that naked mole rats have their reproduction restricted to one queen and less than three males;the rest of the animals within the colony remain sterile. The study aimed at establishing whether the reproductive suppression in subordinate naked mole rats is the outcome of social stress exerted by breeding female in the colony or other unknown environmental factors within the colony set-up. Experimental period was 20 weeks and it involved two stages, acclimatization and isolation. Before acclimation, 2 female subordinate naked mole rats were sacrificed. Blood samples were collected and appropriately preserved for later analysis. On the 2nd, 4th and 6th weeks of acclimatization, 2 female mole rats were sacrificed at each stage and blood samples were harvested for analysis. On week 7, eighteen subordinate females were isolated and randomly assigned into two groups of 9 animals each in two separate cages for 14 weeks. One week 8, 2 subordinate females from each group were randomly selected, sacrificed and blood samples collected for analysis. The same procedure was repeated on week 12 and 20 of study. Hormonal analysis was done using ELISA technique. The Statistical analysis done using student t-test at 95% confidence level and significance level was set at P < 0.05. Results showed increase in cortisol levels only in singly isolated females which were the greatest on week 8 (from 35.5 ng/ml to 123.4 ng/ml). Mean estradiol in singly isolated females was 18 ± 2.52 pg/ml while females paired with males had 19.0 ± 3.0 pg/ml hence no significant difference (paired t = -1.73, P = 0.23). Conclusively, the observed reproductive suppression among subordinate naked mole rats in natal colonies is probably due to presence of queen or other reproductive aspects that may not be directly related to stress.

Improving Transportation and Transplanting Efficiency of Flue-cured Tobacco by Transportation of Naked Seedlings

[Objective] The aim was to resolve the problem of low transportation efficiency of seedlings,vulnerability of seedling trays and difficulty of recycling.[Method]Naked seedlings were transported with trays and frames to improve transportation and transplanting efficiency.[Result] The transportation of naked seedlings would effectively reduce transportation cost and improve transportation and transplanting efficiency of tobacco seedlings.[Conclusion] The transportation of naked seedlings is suitable to be applied in the region with professional transplanting.

Language features in The naked and the dead

As to the theme of human nature, there are many scholars and critics approaching The naked and the dead from different perspectives. This paper aims to analyze this theme from the writer＇s skills in manipulating language, presenting his characters＇ true states of mind. In the novel, Mailer skillfully chooses words and sentences with connotations to expose their human nature. With his choice of words, Mailer further molds his characters, investing them with the richest personalities. By the choice of words, Norman Mailer displays the richest personalities in his characters, disclosing the degeneration of human beings.

A Corpus-based Analysis of the Usage of Naked Eye

It is widely accepted that eye is a high-frequency word in English,and one of the stronger collocations is naked eye.In order to research the usage of naked eye,a concordance line of naked eye is selected from corpus evidence.The collocation,meaning,attitude words and larger variable expression of naked eye are mainly examined with the purpose of better studying the usage of naked eye.

The ring-shaped shadow of a rotating naked singularity with a complete photon sphere

We investigate the shadows of the Konoplya-Zhidenko naked singularity.In the spacetime of the Konoplya-Zhidenko naked singularity,not only an unstable retrograde light ring(LR)but also an unstable prograde LR exists,leading to the formation of a complete photon sphere(PS).Due to the absence of an event horizon,a dark discshaped shadow does not appear;instead,a ring-shaped shadow is observed.The ring-shaped shadow appears as an infinite number of relativistic Einstein rings in the image of the naked singularity.For some parameter values,only the unstable retrograde LR exists,resulting in an incomplete unstable PS and thus giving rise to an arc-shaped shadow for the Konoplya-Zhidenko naked singularity.The shadow of the Konoplya-Zhidenko naked singularity gradually shifts to the right as the rotation parameter a increases and gradually becomes smaller as the deformation parameter |η| increases.Moreover,stable LRs and stable photon spherical orbits can exist in the Konoplya-Zhidenko naked singularity spacetime,but they have no effect on the image of the naked singularity.This study demonstrates that a rotating naked singularity can exhibit not only an arc-shaped shadow but also a ring-shaped shadow.

Clinical efficacy of femtosecond laser-assisted phacoemulsification in diabetic cataract patients

BACKGROUND Diabetic patients with cataracts encounter specific difficulties during cataract surgery due to alterations in microcirculation,blood supply,metabolism,and the microenvironment.Traditional phacoemulsification may not fully tackle these issues,especially in instances with substantial preoperative astigmatism.The utilization of femtosecond laser-assisted phacoemulsification,in conjunction with Toric intraocular lens(IOL)implantation,offers a potentially more efficient strategy.This research seeks to evaluate the efficacy and possible complications of this approach in diabetic cataract patients.AIM To investigate the clinical efficacy and complications of femtosecond laser-assisted phacoemulsification combined with Toric IOL implantation in diabetic cataract patients,comparing it with traditional phacoemulsification methods.METHODS This retrospective study enrolled 120 patients with diabetes cataract from May 2019 to May 2021.The patients were divided into two groups:the control group underwent traditional phacoemulsification and Toric IOL implantation,while the treatment group received Len Sx femtosecond laser-assisted treatment.Outcome measures included naked eye vision,astigmatism,high-level ocular phase difference detection,clinical efficacy,and complication.RESULTS There were no significant preoperative differences in astigmatism or naked eyesight between the two groups.However,postoperative improvements were observed in both groups,with the treatment group showing greater enhancements in naked eye vision and astigmatism six months after the procedure.High-level corneal phase difference tests also indicated significant differences in favor of the treatment group.CONCLUSION This study suggests that femtosecond laser-assisted phacoemulsification combined with Toric IOL implantation appears to be more effective in enhancing postoperative vision in diabetic cataract patients compared to traditional methods offering valuable insights for clinical practice.

Mineralogy of the otoliths of naked carp Gymnocypris przewalskii (Kessler) from Lake Qinghai and its Sr/Ca potential implications for migratory pattern

Otoliths are biogenic carbonate minerals whose microstructure and microchemistry have been used for age determination, stock identification, life history and environmental tracing. Using X-ray diffraction （XRD） and Fourier transform infrared （FTIR） spectroscopy, we have determined the mineral types and crystalline characteristics of three pairs of otoliths from naked carp Gymnocypris przewalskii, the predominant fish in Lake Qinghai. The results indicate that the mineral of both lapillus and sagitta of the naked carp is aragonite, and that of asteriscus is vaterite. The aragonite of lapillus has prefect crystallization. Given the shape of lapillus and the sensitivity of its aragonite to water chemistry, lapillus was used to analyze temporal Sr/Ca ratios along the maximal growth axis by an electron probe microanalyzer （EPMA）. Consistent variations of Sr/Ca ratios with a range of 1.0x10-3-5.0~10-3 on the long and short radii indicate that Sr/Ca ratios of lapillus potentially respond to the chemical compositions of the host waters during the period of the naked carp＇s growth and migration. Discontinuous （dark） zones of lapillus were formed during fall and winter when the naked carp grows slowly in Lake Qinghai, resulting in similar low Sr/Ca ratios to lake water, whereas incremental zones with higher St/Ca ratios respond to its migratory river waters during spring and summer. Various Sr/Ca ratios of incremental zones suggest that the migratory pattern of the naked carp may be much more flexible, rather than in a single river. Therefore, high-resolution otolith microchemistry of the naked carp can be used to trace its migratory behavior, which is of significance for determining its migratory pattern and life history of this precious species inhabiting the Tibetan Plateau.

Stratigraphy and otolith microchemistry of the naked carp Gymnocypris przewalskii(Kessler) and their indication for water level of Lake Qinghai during the Ming Dynasty of China

Otoliths are biogenic carbonate minerals in the inner ear of teleost fish, whose compositions can record the physical and chem- ical conditions of the ambient water environment inhabited by individual fish. In this research, the fishbones and otoliths of naked carp sampled near the Bird Island, offshore Lake Qinghai, were dated and analyzed for mineralogy and microchemical compositions. Comparing the microchemical compositions of ancient otoliths with those of modem otoliths, we conclude that the ancient naked carps inhabited a relict lake formed when the lake shrank from a high lake level, by combining with the AMS-lnC ages of fishbones and otoliths, the stratigraphy and surrounding topography of the sample site. AMS-14C dating resuits of ancient fishbones and otoliths show that these naked carps lived from 680 to 300 years ago, i.e. during the Ming Dynasty of China. The X-ray diffraction （XRD） patterns demonstrate that the ancient lapillus is composed of pure aragonite, iden- tical to modem one, indicating that the mineral of lapillus didn＇t change after a long time burial and that the ancient lapillus is suitable for comparative analysis thereafter. Microchemical results show that both ratios of Mg/Ca （（70.12±18.50）× 10-2） and δ18O （（1.76±1.03）‰） of ancient lapilli are significantly higher than those of modem lapilli （average Mg/Ca=（3.1 1±0.41）× 10-5 and δ18O =（-4.82±0.96）‰）. This reflects that the relict water body in which the ancient naked carp lived during the Ming Dynasty was characterized by higher Mg/Ca and δ18O ratios than modem Lake Qinghai, resulting from strong evaporation after being isolated from the main lake, similar to today＇s Lake Gahai. Based upon the stratigraphy and altitude of naked carp re- mains, it can be inferred that the altitude of lake level of Lake Qinghai reached at least 3202 m with a lake area of 4480 km2 during the Ming Dynasty, approximately ~5% larger than it is today.

EXPERIMENTAL TEST FOR JUPITER'S SATELLITES TO THE NAKED EYE

People always think that the first man who discovered the Jovian satellites was G. Galileo. Last year, the Chinese historian of astronomy Xi Zezong pointed out that the first discovery of the Jovian satellites was made by Gan De, one of the earliest astronomers of China in the summer of 364 B.C.—almost 2,000 years before Galileo’s discovery. At first glance, it seems easy to tell the true from the false. In fact, many people lost their confidence when observing with the naked eye. The reason is very simple: The most elementary conditions for astronomical observation have been

Assessment of naked mole-rat distribution and threats in Eastern Ethiopia

Objective:To identify the distribution,threats and community attitudes towards naked mole-rat in Eastern Ethiopia.Methods:Data were collected through direct observation and interview and Chi-square at 95%confidence interval was used for significance test.Results:Naked mole-rat was identified in Fafan,City/Shinele,Eastern Hararghe Zone and Dire Dawa Administrative.The main threats of naked mole-rat identified were agricultural expansion,human killing and lack of awareness.From a total of 100 respondents,92%of them considered naked mole-rat as pest as a result that 46%of them participated in direct killing.Literacy rate significantly affects the willingness to participate in the conservation of naked mole-rat(χ^(2)=7.478,df=1,P<0.05).From a total of 26%respondents who did not show the willingness to participate in the conservation,80.8%of them were illiterate.Conclusions:Naked mole-rat is fairly common in many of the study sites.However,rapid shift from nomadic life style to cultivation of crops and lacks of awareness were the main threats of naked mole-rat.Therefore,since there is no conservation action currently,further comprehensive study is required to design conservation plan for this species.

Naked Man Scares Shark to Death?

只闻人遭鲨鱼的袭击而亡而伤的故事,未闻鲨鱼被人吓死的消息。不信, 请读此文。

Hacker Posts Naked Pics on Mandela's Fund Web Site

从南非约翰内斯堡传来了电脑黑客“作孽”的消息。黑客将半裸女孩的形象“泼”(注:这个“泼”字借于下文中的splashed)到了曼德拉儿童基金会的网站上!

Women Dance Naked for Rain

选注者言:我刚刚给贵刊发了Women Bare All in Australian Anti-War Protest-文(编者按:此文见今年第3期),又在网上读到本文。开始,我有点不相信自己的眼睛,怎么澳大利亚的女性为Bare/Naked着了魔?怎么动不动就赤身裸体?Healy最后的解释好像有点道理。

Simmered Slips of Naked Oats

IN 1968, I, a middle school graduate, went to live and work as a farmer in Dongru, a small mountain village in north China. Later, I became a shop assistant in the county town. I spent nine years of my life there—from age 17 to 26. The memory of those years has begun to fade; only some words are left here as a reminder of my youth. In May, 1971, the county’s settlement office began transferring the urban educated youth from the countryside into the county-town.