Molecular Cloning of IGFBP-1 Gene and Developmental Expression of Its mRNA in Different Tissues of Nanjiang Mongolian Gazelles

The objective of the present study was to investigate the developmental expression patterns of Insulin-like growth factor-binding protein 1 (IGFBP-1) gene in different tissues of postnatal Nanjiang Mongolian Gazelles. Samples of heart, liver, spleen, lung, longissimus dorsi, semimembranosus, m. triceps brachii and biceps muscle of thigh were collected from a total of 36 Nanjiang Mongolian Gazelles at the age of 0, 15, 30, 60, 90 and 120 days after birth (3 males and 3 females at each age). The CDS was sequenced and ontogeny of mRNA levels of IGFBP-1 were measured by real-time fluorescence quantitative RT-PCR. The size of IGFBP-1 ORF was 792 bp encoding 263 amino acid residues, and displayed higher nucleotide/amino acid sequence identities with other ruminants compared to non-ruminants. The levels of IGFBP-1 mRNA in liver were highest (P<0.01), levels were medium in lung, spleen and heart, and the lowest in the muscles; there were no significant differences among the muscles (P>0.05). Three expression patterns of IGFBP-1 mRNA during postnatal growth from birth to day 60 were found: consistently decreasing (liver), fluctuating as increasing then decreasing (heart) or as decreasing then increasing then decreasing (spleen, lung and muscles). The results indicate that the IGFBP-1 gene is highly conserved among species, and liver has the highest expression. It was concluded that IGFBP-1 plays important roles in early postnatal growth and is expressed in a developmental-tissue-dependent manner.

国家级茶树新品种——“南江1号”

南江1号以南江大叶茶群体品种为原始材料,采用系统选种法育成,历经10年的品比试验和6年的全国区域试验,结果表明,南江1号为早生种,适制绿茶,品质、产量都优于对照福鼎大白茶,抗性强。2010年8月通过鉴定,成为新的国家级无性系茶树良种。

南江黄羊FSHβ亚基基因内含子1序列的测定及分析

利用GenBank中公布的牛、绵羊等动物FSHβ亚基基因序列设计引物,采用PCR法扩增并测定出南江黄羊FSHβ亚基基因内含子1(in-1)全序列(GenBank登录号为AY838283)。山羊FSHβ基因内含子1全序列长641 bp,A、C、G、T4种碱基分别占35．73%、13．88%、17．47%、32．92%,A+T (68．65%)的含量远高于G+C(31．35%)的含量。不同物种间FSHβ基因序列相似性的变异范围为24．1%～97．3%,分歧度则为2．6%～85．7%。利用FSHβ亚基基因内含子1序列进行的物种间系统发育分析结果与实际的物种演化顺序一致,表明它可用于物种间的系统发育分析。

南江黄羊FSHβ亚基基因内含子1序列的测定

利用牛、绵羊等动物FSHβ亚基基因序列设计引物,采用PCR法扩增并测定出南江黄羊FSHβ亚基基因内含子1全序列(GenBank登录号:AY838283)。山羊FSHβ基因内含子1全序列长641bp,A、C、G、T 4种碱基分别占35.73%,13.88%,17.47%,32.92%;A+T(68.65%)的含量远高于G+C(31.35%)的含量。所测定7个个体的碱基序列完全一致,表明FSHβ基因内含子1的保守性较强。