For circulating tumor cells(CTCs)-based cancer diagnosis and monitoring,effective enrichment and specific analysis of CTCs present significant challenges.The biomembrane interfaces can enhance the highaffinity interac...For circulating tumor cells(CTCs)-based cancer diagnosis and monitoring,effective enrichment and specific analysis of CTCs present significant challenges.The biomembrane interfaces can enhance the highaffinity interactions between various receptors and ligands in life activities by mediating the rearrangement and positioning of membrane-bound components through its fluidity.Inspired by this,we have constructed a multivalent membrane nano-interface using aptamer-linked liposomes for the efficient capture of CTCs.Furthermore,the subsequent introduction of rolling circle amplification(RCA)reaction has increased the number of aptamers and extended them to the surrounding space to improve the affinity of the membrane nano-interface for CTCs.After CTCs are enriched,alkaline phosphatase overexpressed on the surface of tumor cells is used as endogenous enzyme-mediated signal amplification by catalyzing 4-nitrophenyl phosphate(p NPP)with color change,achieving the analysis of CTCs.Finally,the enrichment and visual analysis of human hepatocellular carcinoma(HepG2)with a detection limit of 10 cells/m L can be obtained by integrating the multivalent membrane nano-interface and endogenous enzyme signal amplification.The detection of the target in the serum proved this method has the potential for further clinical application and provides a potential method for studying the correlation between alkaline phosphatase dimer and cancer progression.展开更多
1 Results The catalysts which can efficiently hydro-reform higher n-paraffin to lower isoparaffins for environmentally-friendly gasoline were studied. The catalysts were examined by the conversion of n-hexadecane, n-C...1 Results The catalysts which can efficiently hydro-reform higher n-paraffin to lower isoparaffins for environmentally-friendly gasoline were studied. The catalysts were examined by the conversion of n-hexadecane, n-C16H34 to i-C6H14—i-C10H22.The tri-modally nano-porous catalysts composed of (Ni-Mo)/[γ-Al2O3], nano-oxide, and nano-crystalline zeolite had some active and selective performance because of the interface between nano-oxide and nano-zeolite. The catalyst composed of nano-crystalline MFI or BE...展开更多
基金supported by the National Natural Science Foundation of China(No.81672570)the State Key Laboratory of Natural and Biomimetic Drugs(No.K202009)。
文摘For circulating tumor cells(CTCs)-based cancer diagnosis and monitoring,effective enrichment and specific analysis of CTCs present significant challenges.The biomembrane interfaces can enhance the highaffinity interactions between various receptors and ligands in life activities by mediating the rearrangement and positioning of membrane-bound components through its fluidity.Inspired by this,we have constructed a multivalent membrane nano-interface using aptamer-linked liposomes for the efficient capture of CTCs.Furthermore,the subsequent introduction of rolling circle amplification(RCA)reaction has increased the number of aptamers and extended them to the surrounding space to improve the affinity of the membrane nano-interface for CTCs.After CTCs are enriched,alkaline phosphatase overexpressed on the surface of tumor cells is used as endogenous enzyme-mediated signal amplification by catalyzing 4-nitrophenyl phosphate(p NPP)with color change,achieving the analysis of CTCs.Finally,the enrichment and visual analysis of human hepatocellular carcinoma(HepG2)with a detection limit of 10 cells/m L can be obtained by integrating the multivalent membrane nano-interface and endogenous enzyme signal amplification.The detection of the target in the serum proved this method has the potential for further clinical application and provides a potential method for studying the correlation between alkaline phosphatase dimer and cancer progression.
基金We acknowledge the National Natural Science Foundation of China(52273110,21975191,52372271 and 22173070)the Youth Top Talent Project of Hubei Provence(GGB)+1 种基金the Knowledge Innovation Program of Wuhan-Shuguang Project(GGB)the Fundamental Research Funds for the Central Universities(WUT:2023III013GX)for financial supports.
文摘1 Results The catalysts which can efficiently hydro-reform higher n-paraffin to lower isoparaffins for environmentally-friendly gasoline were studied. The catalysts were examined by the conversion of n-hexadecane, n-C16H34 to i-C6H14—i-C10H22.The tri-modally nano-porous catalysts composed of (Ni-Mo)/[γ-Al2O3], nano-oxide, and nano-crystalline zeolite had some active and selective performance because of the interface between nano-oxide and nano-zeolite. The catalyst composed of nano-crystalline MFI or BE...
