Objective: To observe the therapeutic effect and mechanism of Naohuandan (脑还丹,NHD) in treating senile dementia (SD).Methods: Clinical study: Fifty-eight patients with SD, whose diagnosis conforms to the Diagnostic ...Objective: To observe the therapeutic effect and mechanism of Naohuandan (脑还丹,NHD) in treating senile dementia (SD).Methods: Clinical study: Fifty-eight patients with SD, whose diagnosis conforms to the Diagnostic Standard of DSM-Ⅳ issued by American Association of Psychiatry, were enrolled and randomly assigned into two groups. The 30 patients in the treated group were treated with NHD, 4 capsules each time, 3 times daily. The 28 patients in the control group were treated with Piracetam, 1.6 g each time, 3 times daily. The therapeutic course for both groups was 3 months. The therapeutic efficacy was estimated and compared by comprehensive scores of memory and cognition, scores of Mini-mental State Examination (MMSE) and Activities of Daily Living (ADL). Experimental study: Rats were divided into the control group, the model group and the high-dosage and low-dosage NHD treated groups. The protective effect of NHD on the per-oxidative damage of hippocampal neurons in β-amyloid protein induced SD model was observed and the related criteria were determined. Results: Clinical study showed that both NHD and Piracetam could improve the clinical symptoms of patients, the two medicines showing insignificant difference in total effective rate. But NHD was better in elevating MMSE score and lowering ADL score in patients than Piracetam (P<0.05 and P<0.01). Experimental study showed that (1) 24 and 72 hrs after modeling, the activity of SOD and GSH were lower and the level of MDA higher in the model group than those in the control group (P<0.05 or P<0.01). Compared with the model group at the corresponding time points, in the high-dosage NHD group, SOD and GSH were higher, MDA was lower (P<0.05 or P<0.01); but in the low-dosage NHD group, SOD at the 72th hr was higher (P<0.05) and MDA at 24th and 72th hrs was lower (P<0.01). And most of the criteria in the high-dosage NHD group was improved better than that in the low-dosage NHD group. (2) The survival rates of neurons in various groups were not different significantly (P>0.05) 24 hrs after modeling, but that in the high-dosage NHD group was significantly higher than that in the model group ( P<0.01) and in the low-dosage NHD group 72 hrs after modeling(P<0.05). Conclusion: NHD is an effective Chinese herbal preparation for treatment of SD, and its mechanism is related with its inhibition on peroxidative injury and protection on neurons.展开更多
目的:探讨脑还丹对由Aβ25-35诱导的实验大鼠Aβ25-35抗体和PC12细胞的凋亡及其炎性因子的影响。方法:将SD大鼠分为对照组、Aβ组、脑还丹高剂量组、脑还丹低剂量组,除对照组外,其余各组分别于实验第1天、第15天和第45天接种Aβ25-35,...目的:探讨脑还丹对由Aβ25-35诱导的实验大鼠Aβ25-35抗体和PC12细胞的凋亡及其炎性因子的影响。方法:将SD大鼠分为对照组、Aβ组、脑还丹高剂量组、脑还丹低剂量组,除对照组外,其余各组分别于实验第1天、第15天和第45天接种Aβ25-35,接种完毕后,中药组予相应药物治疗105d,各组分别在每次接种后第7天取血检测Aβ25-35抗体滴度。另外,培养PC12细胞,分组同上,除对照组外,各组分别加入Aβ25-35,同时中药组加入相对应含药血清,培养72h后检测bcl-x L mRNA和IL-1β、IL-6、TNF-α蛋白含量。结果:各组抗体量均高于对照组(P<0.01),脑还丹高剂量组产生抗体量高于Aβ组与脑还丹低剂量组(P<0.05);Aβ组IL-1β、IL-6、TNF-α分泌量均显著高于对照组(P<0.01),脑还丹低剂量组、Aβ组的IL-1β、IL-6、TNF-α分泌量均高于脑还丹高剂量组(P<0.01);Aβ组与脑还丹低剂量组bcl-x L mRNA的表达较对照组和脑还丹高剂量组低(P<0.01)。结论:脑还丹具有促进特异性Aβ25-35抗体产生的作用,同时其抗凋亡作用可能是通过降低IL-1β、IL-6、TNF-α炎性因子的水平实现的,并呈剂量依赖性。展开更多
基金the Research Fund from Guangdong Provincial Administration of TCM (No. A002003002 )
文摘Objective: To observe the therapeutic effect and mechanism of Naohuandan (脑还丹,NHD) in treating senile dementia (SD).Methods: Clinical study: Fifty-eight patients with SD, whose diagnosis conforms to the Diagnostic Standard of DSM-Ⅳ issued by American Association of Psychiatry, were enrolled and randomly assigned into two groups. The 30 patients in the treated group were treated with NHD, 4 capsules each time, 3 times daily. The 28 patients in the control group were treated with Piracetam, 1.6 g each time, 3 times daily. The therapeutic course for both groups was 3 months. The therapeutic efficacy was estimated and compared by comprehensive scores of memory and cognition, scores of Mini-mental State Examination (MMSE) and Activities of Daily Living (ADL). Experimental study: Rats were divided into the control group, the model group and the high-dosage and low-dosage NHD treated groups. The protective effect of NHD on the per-oxidative damage of hippocampal neurons in β-amyloid protein induced SD model was observed and the related criteria were determined. Results: Clinical study showed that both NHD and Piracetam could improve the clinical symptoms of patients, the two medicines showing insignificant difference in total effective rate. But NHD was better in elevating MMSE score and lowering ADL score in patients than Piracetam (P<0.05 and P<0.01). Experimental study showed that (1) 24 and 72 hrs after modeling, the activity of SOD and GSH were lower and the level of MDA higher in the model group than those in the control group (P<0.05 or P<0.01). Compared with the model group at the corresponding time points, in the high-dosage NHD group, SOD and GSH were higher, MDA was lower (P<0.05 or P<0.01); but in the low-dosage NHD group, SOD at the 72th hr was higher (P<0.05) and MDA at 24th and 72th hrs was lower (P<0.01). And most of the criteria in the high-dosage NHD group was improved better than that in the low-dosage NHD group. (2) The survival rates of neurons in various groups were not different significantly (P>0.05) 24 hrs after modeling, but that in the high-dosage NHD group was significantly higher than that in the model group ( P<0.01) and in the low-dosage NHD group 72 hrs after modeling(P<0.05). Conclusion: NHD is an effective Chinese herbal preparation for treatment of SD, and its mechanism is related with its inhibition on peroxidative injury and protection on neurons.
文摘目的:探讨脑还丹对由Aβ25-35诱导的实验大鼠Aβ25-35抗体和PC12细胞的凋亡及其炎性因子的影响。方法:将SD大鼠分为对照组、Aβ组、脑还丹高剂量组、脑还丹低剂量组,除对照组外,其余各组分别于实验第1天、第15天和第45天接种Aβ25-35,接种完毕后,中药组予相应药物治疗105d,各组分别在每次接种后第7天取血检测Aβ25-35抗体滴度。另外,培养PC12细胞,分组同上,除对照组外,各组分别加入Aβ25-35,同时中药组加入相对应含药血清,培养72h后检测bcl-x L mRNA和IL-1β、IL-6、TNF-α蛋白含量。结果:各组抗体量均高于对照组(P<0.01),脑还丹高剂量组产生抗体量高于Aβ组与脑还丹低剂量组(P<0.05);Aβ组IL-1β、IL-6、TNF-α分泌量均显著高于对照组(P<0.01),脑还丹低剂量组、Aβ组的IL-1β、IL-6、TNF-α分泌量均高于脑还丹高剂量组(P<0.01);Aβ组与脑还丹低剂量组bcl-x L mRNA的表达较对照组和脑还丹高剂量组低(P<0.01)。结论:脑还丹具有促进特异性Aβ25-35抗体产生的作用,同时其抗凋亡作用可能是通过降低IL-1β、IL-6、TNF-α炎性因子的水平实现的,并呈剂量依赖性。
