Ultraviolet radiation by its wavelength is divided into: UVA, UVB and UVC. Only UVA and UVB manage to penetrate the ozone layer, but due to anthropological activities, all of them are capable of interacting with human...Ultraviolet radiation by its wavelength is divided into: UVA, UVB and UVC. Only UVA and UVB manage to penetrate the ozone layer, but due to anthropological activities, all of them are capable of interacting with humans to a greater or lesser extent, and can generate adverse effects such as cellular stress when interacting with intra-and extracellular biomolecules. The skin is the first organ in contact with UV radiation, and the stress it generates can be analyzed by the expression of a bioindicator of cellular damage such as Hsp70. Therefore, the objective of the project was: to determine the effect of UVA, UVB and UVC radiation on HaCaT epithelial cells, by analyzing the expression of Hsp70. Materials and methods: HaCaT cells were cultured in vitro, which were irradiated with UVA, UVB and UVC light at different doses, to subsequently determine the degree of Hsp70 expression by Immunodetection by PAGE-SDS and Western Blot. Results: Basal expression of Hsp70 was observed in no irradiated HaCaT cells. When HaCaT cells were irradiated with UVA, UVB, UVC, an increase in this Hsp70 protein was observed. With UVA, a higher degree of expression was observed at a time of 30 minutes of irradiation. With UVB the highest expression shifted to a time of 20 minutes. With UVC, overexpression was observed after 10 minutes. Conclusion: UV radiation generates cellular stress on HaCaT cells, evaluated by the stress bioindicator Hsp70. According to the wavelength of UV radiation, those that have a shorter wavelength have a greater potential for cellular damage, such as UVC.展开更多
Exposure of skin to solar ultraviolet（UV） radiation induces photo-damage.Ultraviolet B（UVB） is the major component of UV radiation which induces the production of reactive oxygen species（ROS） and plays an impor-...Exposure of skin to solar ultraviolet（UV） radiation induces photo-damage.Ultraviolet B（UVB） is the major component of UV radiation which induces the production of reactive oxygen species（ROS） and plays an impor-tant role in photo-damage.Hydrogen gas reduces ROS and alleviates inflammation.In this study,we sought to demonstrate that hydrogen-rich saline has the effect on skin injuries caused by UVB radiation.UVB radiation was irradiated on female C57BL/6 rats to induce skin injury.Hydrogen-rich saline and nitrogen-rich saline were ad-ministered to rats by intraperitoneal injection.Skin damage was detected by microscope after injury.UVB radia-tion had a significant affection in tumor necrosis factor alpha,interleukin（IL）-1β and IL-6 levels,tissue superox-ide dismutase,malondialdehyde and nitric oxide activity.Hydrogen-rich saline had a protective effect by altering the levels of these markers and relieved morphological skin injury.Hydrogen-rich saline protected against UVB radiation injury,possibly by reducing inflammation and oxidative stress.展开更多
Effect of cerium （Ce^3＋） on growth and photosynthesis in rape seedlings exposed to two levels of ultraviolet-B radiation （UV-B, 280 - 320 nm） was studied with hydroponics under laboratory conditions. The growth o...Effect of cerium （Ce^3＋） on growth and photosynthesis in rape seedlings exposed to two levels of ultraviolet-B radiation （UV-B, 280 - 320 nm） was studied with hydroponics under laboratory conditions. The growth of rape seedlings exposed to two levels of UV-B irradiation （0.15 and 0.35 W· m^-2/T2） was both heavily restrained. The aboveground growth indices including stem （plant） height, leaf number, leaf area, leaf fresh/dry weight and stem fresh/dry weight were obviously decreased by 13.2% - 44.1% （T1） and 21 .4% - 49.3% （T2）. Compared to CK, and except active absorption area of roots, the belowground indices main root length, root volume and fresh/dry weight by 14.1% -35.6% （T1） and 20.3% - 42.6% （T2）, respectively. For Ce ＋ UV-B treatments, the aboveground and belowground growth indices were decreased by 4.1% - 23.6%, 5.2% -23.3% （Ce＋T1） and 10.8% -28.4%, 7.0% -27.8% （Ce ＋T2）, lower than those of UV-B treatments mentioned above. These results show that Ce has protective effect on plant against injury of UV-B radiation. Furthermore, the protective effect of Ce on seedlings exposed to T1 level of UV-B radiation is superior to T2 level. Chlorophyll content, net photosynthesis rate, transpiration rate, stomatal conductance and water use efficiency in UV-B treatments decrease dramatically, whereas intercellular CO2 concentration increases. Although these indices in Ce ＋ UV-B treatments decrease compared with those of CK, the decrease in Ce ＋ UV-B treatments are lower than those in UV-B treatment. This phenomenon indicates that the ecophysiological protective effect of Ce is based on improving photosynthesis in plants. The dynamic curves of photosynthesis indices show that the course of light-repair is shortened and the injury to rape seedlings by UV-B radiation stress is alleviated by Ce.展开更多
The effect of La on flavonoids, chlorophyll contents, and phenylalanine ammonia-lyase （PAL） activity in soybean seedlings under supplementary ultraviolet-B radiation （UV-B, 280 - 320 nm） was studied. The results s...The effect of La on flavonoids, chlorophyll contents, and phenylalanine ammonia-lyase （PAL） activity in soybean seedlings under supplementary ultraviolet-B radiation （UV-B, 280 - 320 nm） was studied. The results show that PAL activity, contents of flavonoids and chlorophyll in the plants pretreated with La （20 mg·L^- 1 ） are higher than those in CK. UV-B radiation could result in an increase in flavonoid content and PAL activity, associated with a decrease in chlorophyll content. However, the increase in the range of PAL activity and flavonoid content in UV-B treatment are lesser than those in the La treatment. The changes of flavonoid contents and PAL activity in La ＋ UV-B treatment are similar to those in UV-B treatment, and the increase in their range is higher than those in UV-B treatment. This shows that La can enhance the resistance of soybean seedling to UV-B radiation and alleviate the damage of UV-B radiation by increasing flavonoid content, chlorophyll content, and PAL activity.展开更多
In order to investigate the effects of lanthanum（Ⅲ） on cell ultrastructure of soybean leaves under elevated ultraviolet-B irradiation （UV-B, 280-320 nm）, the chloroplast ultrastructure of soybean seedlings was st...In order to investigate the effects of lanthanum（Ⅲ） on cell ultrastructure of soybean leaves under elevated ultraviolet-B irradiation （UV-B, 280-320 nm）, the chloroplast ultrastructure of soybean seedlings was studied by hydroponics under laboratory conditions. The resuits showed that the thylakoid in chloroplast was orderly and clearly as soybean leaves were pretreated by La（Ⅲ）. The thylakoid was indis- tinctly disordered, expanded and even indiscoverable in the chloroplast under UV-B stress. The impact on the thylakoid by the high intensity UV-B irradiation （T2） was bigger than that by the low intensity UV-B irradiation （T1）. However, the destruction of the chloroplast structure caused by UV-B stress was alleviated by La（Ⅲ）, and the arrangement of the thylakoid in the chloroplast became orderly and clearly. The effect of the alleviation by La（Ⅲ） under the low intensity UV-B irradiation （T1） was better than that under the high intensity UV-B irradiation （T2）.展开更多
Ultraviolet radiation-B (UVBR) affects plants in many important ways, including reduced growth rate, reduction of primary productivity and changes in ultrastructure. The rice (Oryza sativa) is one of the most cultivat...Ultraviolet radiation-B (UVBR) affects plants in many important ways, including reduced growth rate, reduction of primary productivity and changes in ultrastructure. The rice (Oryza sativa) is one of the most cultivated cereal in the world along with corn and wheat, representing over 50% of agricultural production. In this study, we examined O. sativa exposed to natural radiation denominated which “ambient samples”, plants cultivated which photosynthetically active radiation (PAR), denominated with PAR-only and plants cultivated with PAR + UVBR for 2 h per day during 30 days of cultivation in vitro. The samples were processed for electron microscopy and histochemistry analysis. PAR + UVBR caused changes in the ultrastructure of leaf of O. sativa, mesophyll cells, which included increased thickness of the cell wall and plastoglobuli, reduced intracellular spaces, changes in the cell contour, and destruction of chloroplast and mitochondria internal organization. The exposure to PAR + UVBR led to changes in guard and subsidiary cells, and the stomata and papillae were with irregular shape. The reduction of epicuticular wax that covered the leaf, was observed. Taken together, these ?ndings strongly suggested that PAR + UVBR negatively affects the ultrastructure and morphology and growth rates, of leaf of O. sativa and, in the long term, their economic viability.展开更多
Vitiligo is a common, acquired pigmentary disorder of unknown etiology with great impact on patient's appearance and quality of life. It presents a therapeutic challenge to many dermatologists. Photochemotherapy u...Vitiligo is a common, acquired pigmentary disorder of unknown etiology with great impact on patient's appearance and quality of life. It presents a therapeutic challenge to many dermatologists. Photochemotherapy using psoralen and ultraviolet A(UVA) therapy, topical and oral immunosuppresants, as well as cosmetic camouflage are also commonly employed with varying clinical efficacy. Phototherapy is a popular treatment option, which includes both of the generalized ultraviolet B(UVB) therapies, broadband UVB and narrowband UVB(NB-UVB). It has been used favorably, both alone as well as in combination with other agents like topical calcineurin inhibitors, vitamin-D analogs. Combination therapies are useful and may provide quicker regimentation and treat vitiligo with an additive mechanism of action than UVB phototherapy. Advances in technology may lead to the continuing use of UVB phototherapy as a treatment for vitiligo through the development of sophisticated devices and delivery systems as well as innovative application methods. These will provide increased therapeutic options for all vitiligo patients, particularly those with refractory disease. In this article, I have reviewed the available data pertaining to efficacy and safety issues for NB-UVB as monotherapy, its comparison with psoralen plus UVA and other modes of phototherapy, combination regimens that have been tried and future prospects of NB-UVB in vitiligo.展开更多
Tropospheric ozone （O3）, ultraviolet B （UVB） radiation and aerosol light scattering coefficients （SC） were investigated on a cruise ship during the fourth Chinese National Arctic Research Expedition from July 1 ...Tropospheric ozone （O3）, ultraviolet B （UVB） radiation and aerosol light scattering coefficients （SC） were investigated on a cruise ship during the fourth Chinese National Arctic Research Expedition from July 1 September 20, 2010. The results showed that O3, UVB and SC decreased with increasing latitude, with minimum values recorded in the central Arctic Ocean. Average O3 concentrations were 15.9 ppbv and 15.1 ppbv in the Bering Sea and Arctic Ocean, respectively. Ozone concentrations increased to 17.5 ppbv in the high Arctic region. Average UVB values were 0.26 W.m-2 and 0.14 W.m-2 in the Bering Sea and Arctic Ocean, respectively. The average SC in the Bering Sea was 4.3 M.m-1, more than twice the value measured in the Arctic Ocean, which had an average value of 1.7 M.m-1. Overall, UVB and SC values were stable in the central Arctic Ocean.展开更多
Wheat (Triticum aestivum), as a kind of important economic crop cultured in the Northern China, is affected by present-day enhanced ultraviolet-B (UV-B) radiation. To get the information of the impact by UV-B radiatio...Wheat (Triticum aestivum), as a kind of important economic crop cultured in the Northern China, is affected by present-day enhanced ultraviolet-B (UV-B) radiation. To get the information of the impact by UV-B radiation on it, the proteins of wheat (Jin mai NO.8) leaves, which were divided into the normal light group (CK) and UV-B radiation group (B), were extracted and ran at SDS-PAGE at different treatment days (5, 6, 7). The proteins were also analyzed by run two-dimensional gel electrophoresis (2-DE), which allowed the identification of some significantly different gel spots. The proteins spots were further verified by Matrix-Assisted Laser Desorption/lonization-time of Flight Mass Spectrometry. The results showed: 1) the enhanced UV-B affects the growth of the wheat, as the visual changes appear on the sixth day;2) the proteins expressions between the B group and the CK group were remarkably different on the sixth day;3) the proteins of wheat leaves of the sixth day were further analyzed by 2-DE revealed that twenty-one protein points were identificated between the B group and the CK group. Among these twenty-one proteins, six proteins of them were up-regulated and twelve proteins of them were down-regulated, three new proteins were expressed only in the B group. Three proteins among six proteins, which were up-regulated, were further verified as RuBisCo large subunit binding protein;SOD;Calmodulin. The result indicates wheat could improve genes encoding proteins in their leaves and protect themselves, when enhanced UV-B affects the growth of the wheat.展开更多
To investigate the protective effect of epigallocatechin gallate(EGCG)on the immune func- tion of dendritic cells(DCs)after ultraviolet B irradiation(UVB)and its underlying mechanisms,the monocytes were isolated from ...To investigate the protective effect of epigallocatechin gallate(EGCG)on the immune func- tion of dendritic cells(DCs)after ultraviolet B irradiation(UVB)and its underlying mechanisms,the monocytes were isolated from peripheral blood and cultivated into DCs with cytokines,such as GM-CSF and IL-4.DCs were harvested after cultivation for 7 d and subjected to irradiation with different dosages of UVB.Then,200μg/ml of EGCG were added in certain groups immediately after irradiation.DCs simply treated with UVB or treated with both UVB and EGCG were co-cultured with lymphocytes,and MTF assay was used to detect the ability of DCs to stimulate proliferation of lymphocytes.Surface markers CDS0,CD86,HLA-DR and CD40 were detected by flow cytometry,and the levels of IL-10 and IL-12 secreted from DCs 24 h after cultivation were measured by ELISA.It was demonstrated that UVB irradiation could inhibit the ability of DCs to stimulate the proliferation of lymphocytes and surface expressions of CDS0,CD86,HLA-DR and CD4 on DCs in a dose-dependent manner.The inhibition rate of DCs was improved to some extent after treatment with 200μg/ml of EGCG.When the concentra- tion of EGCG exceeded 100μg/ml,the enhancing effect of EGCG on the expression of the co-stimulat- ing molecules on DCs could be demonstrated in a dose-dependent manner.UVB showed no significant influence on the secretion of IL-10 and IL-12 from DCs,while EGCG could down-regulate the secretion level of IL-12 and up-regulate that of IL-10.It is concluded that EGCG can antagonize the inhibitory effect on DCs induced by UVB irradiation.This function has some relationship with its protecting effect of the expression of the co-stimulating molecule on the surface of DCs and the secretion level of IL-10 and IL-12.展开更多
文摘Ultraviolet radiation by its wavelength is divided into: UVA, UVB and UVC. Only UVA and UVB manage to penetrate the ozone layer, but due to anthropological activities, all of them are capable of interacting with humans to a greater or lesser extent, and can generate adverse effects such as cellular stress when interacting with intra-and extracellular biomolecules. The skin is the first organ in contact with UV radiation, and the stress it generates can be analyzed by the expression of a bioindicator of cellular damage such as Hsp70. Therefore, the objective of the project was: to determine the effect of UVA, UVB and UVC radiation on HaCaT epithelial cells, by analyzing the expression of Hsp70. Materials and methods: HaCaT cells were cultured in vitro, which were irradiated with UVA, UVB and UVC light at different doses, to subsequently determine the degree of Hsp70 expression by Immunodetection by PAGE-SDS and Western Blot. Results: Basal expression of Hsp70 was observed in no irradiated HaCaT cells. When HaCaT cells were irradiated with UVA, UVB, UVC, an increase in this Hsp70 protein was observed. With UVA, a higher degree of expression was observed at a time of 30 minutes of irradiation. With UVB the highest expression shifted to a time of 20 minutes. With UVC, overexpression was observed after 10 minutes. Conclusion: UV radiation generates cellular stress on HaCaT cells, evaluated by the stress bioindicator Hsp70. According to the wavelength of UV radiation, those that have a shorter wavelength have a greater potential for cellular damage, such as UVC.
文摘Exposure of skin to solar ultraviolet（UV） radiation induces photo-damage.Ultraviolet B（UVB） is the major component of UV radiation which induces the production of reactive oxygen species（ROS） and plays an impor-tant role in photo-damage.Hydrogen gas reduces ROS and alleviates inflammation.In this study,we sought to demonstrate that hydrogen-rich saline has the effect on skin injuries caused by UVB radiation.UVB radiation was irradiated on female C57BL/6 rats to induce skin injury.Hydrogen-rich saline and nitrogen-rich saline were ad-ministered to rats by intraperitoneal injection.Skin damage was detected by microscope after injury.UVB radia-tion had a significant affection in tumor necrosis factor alpha,interleukin（IL）-1β and IL-6 levels,tissue superox-ide dismutase,malondialdehyde and nitric oxide activity.Hydrogen-rich saline had a protective effect by altering the levels of these markers and relieved morphological skin injury.Hydrogen-rich saline protected against UVB radiation injury,possibly by reducing inflammation and oxidative stress.
文摘Effect of cerium （Ce^3＋） on growth and photosynthesis in rape seedlings exposed to two levels of ultraviolet-B radiation （UV-B, 280 - 320 nm） was studied with hydroponics under laboratory conditions. The growth of rape seedlings exposed to two levels of UV-B irradiation （0.15 and 0.35 W· m^-2/T2） was both heavily restrained. The aboveground growth indices including stem （plant） height, leaf number, leaf area, leaf fresh/dry weight and stem fresh/dry weight were obviously decreased by 13.2% - 44.1% （T1） and 21 .4% - 49.3% （T2）. Compared to CK, and except active absorption area of roots, the belowground indices main root length, root volume and fresh/dry weight by 14.1% -35.6% （T1） and 20.3% - 42.6% （T2）, respectively. For Ce ＋ UV-B treatments, the aboveground and belowground growth indices were decreased by 4.1% - 23.6%, 5.2% -23.3% （Ce＋T1） and 10.8% -28.4%, 7.0% -27.8% （Ce ＋T2）, lower than those of UV-B treatments mentioned above. These results show that Ce has protective effect on plant against injury of UV-B radiation. Furthermore, the protective effect of Ce on seedlings exposed to T1 level of UV-B radiation is superior to T2 level. Chlorophyll content, net photosynthesis rate, transpiration rate, stomatal conductance and water use efficiency in UV-B treatments decrease dramatically, whereas intercellular CO2 concentration increases. Although these indices in Ce ＋ UV-B treatments decrease compared with those of CK, the decrease in Ce ＋ UV-B treatments are lower than those in UV-B treatment. This phenomenon indicates that the ecophysiological protective effect of Ce is based on improving photosynthesis in plants. The dynamic curves of photosynthesis indices show that the course of light-repair is shortened and the injury to rape seedlings by UV-B radiation stress is alleviated by Ce.
基金Project supported by the National Natural Science Foundation of China (20471030)the Foundation of State PlanningCommittee (IFZ20051210)
文摘The effect of La on flavonoids, chlorophyll contents, and phenylalanine ammonia-lyase （PAL） activity in soybean seedlings under supplementary ultraviolet-B radiation （UV-B, 280 - 320 nm） was studied. The results show that PAL activity, contents of flavonoids and chlorophyll in the plants pretreated with La （20 mg·L^- 1 ） are higher than those in CK. UV-B radiation could result in an increase in flavonoid content and PAL activity, associated with a decrease in chlorophyll content. However, the increase in the range of PAL activity and flavonoid content in UV-B treatment are lesser than those in the La treatment. The changes of flavonoid contents and PAL activity in La ＋ UV-B treatment are similar to those in UV-B treatment, and the increase in their range is higher than those in UV-B treatment. This shows that La can enhance the resistance of soybean seedling to UV-B radiation and alleviate the damage of UV-B radiation by increasing flavonoid content, chlorophyll content, and PAL activity.
基金supported by the National Natural Science Foundation of China (30570323)the Special Foundation for Rare Earth of State Planning Committee (IFZ20051210)
文摘In order to investigate the effects of lanthanum（Ⅲ） on cell ultrastructure of soybean leaves under elevated ultraviolet-B irradiation （UV-B, 280-320 nm）, the chloroplast ultrastructure of soybean seedlings was studied by hydroponics under laboratory conditions. The resuits showed that the thylakoid in chloroplast was orderly and clearly as soybean leaves were pretreated by La（Ⅲ）. The thylakoid was indis- tinctly disordered, expanded and even indiscoverable in the chloroplast under UV-B stress. The impact on the thylakoid by the high intensity UV-B irradiation （T2） was bigger than that by the low intensity UV-B irradiation （T1）. However, the destruction of the chloroplast structure caused by UV-B stress was alleviated by La（Ⅲ）, and the arrangement of the thylakoid in the chloroplast became orderly and clearly. The effect of the alleviation by La（Ⅲ） under the low intensity UV-B irradiation （T1） was better than that under the high intensity UV-B irradiation （T2）.
文摘Ultraviolet radiation-B (UVBR) affects plants in many important ways, including reduced growth rate, reduction of primary productivity and changes in ultrastructure. The rice (Oryza sativa) is one of the most cultivated cereal in the world along with corn and wheat, representing over 50% of agricultural production. In this study, we examined O. sativa exposed to natural radiation denominated which “ambient samples”, plants cultivated which photosynthetically active radiation (PAR), denominated with PAR-only and plants cultivated with PAR + UVBR for 2 h per day during 30 days of cultivation in vitro. The samples were processed for electron microscopy and histochemistry analysis. PAR + UVBR caused changes in the ultrastructure of leaf of O. sativa, mesophyll cells, which included increased thickness of the cell wall and plastoglobuli, reduced intracellular spaces, changes in the cell contour, and destruction of chloroplast and mitochondria internal organization. The exposure to PAR + UVBR led to changes in guard and subsidiary cells, and the stomata and papillae were with irregular shape. The reduction of epicuticular wax that covered the leaf, was observed. Taken together, these ?ndings strongly suggested that PAR + UVBR negatively affects the ultrastructure and morphology and growth rates, of leaf of O. sativa and, in the long term, their economic viability.
文摘Vitiligo is a common, acquired pigmentary disorder of unknown etiology with great impact on patient's appearance and quality of life. It presents a therapeutic challenge to many dermatologists. Photochemotherapy using psoralen and ultraviolet A(UVA) therapy, topical and oral immunosuppresants, as well as cosmetic camouflage are also commonly employed with varying clinical efficacy. Phototherapy is a popular treatment option, which includes both of the generalized ultraviolet B(UVB) therapies, broadband UVB and narrowband UVB(NB-UVB). It has been used favorably, both alone as well as in combination with other agents like topical calcineurin inhibitors, vitamin-D analogs. Combination therapies are useful and may provide quicker regimentation and treat vitiligo with an additive mechanism of action than UVB phototherapy. Advances in technology may lead to the continuing use of UVB phototherapy as a treatment for vitiligo through the development of sophisticated devices and delivery systems as well as innovative application methods. These will provide increased therapeutic options for all vitiligo patients, particularly those with refractory disease. In this article, I have reviewed the available data pertaining to efficacy and safety issues for NB-UVB as monotherapy, its comparison with psoralen plus UVA and other modes of phototherapy, combination regimens that have been tried and future prospects of NB-UVB in vitiligo.
基金supported by the project"Fourth Chinese National Arctic Research Expedition"
文摘Tropospheric ozone （O3）, ultraviolet B （UVB） radiation and aerosol light scattering coefficients （SC） were investigated on a cruise ship during the fourth Chinese National Arctic Research Expedition from July 1 September 20, 2010. The results showed that O3, UVB and SC decreased with increasing latitude, with minimum values recorded in the central Arctic Ocean. Average O3 concentrations were 15.9 ppbv and 15.1 ppbv in the Bering Sea and Arctic Ocean, respectively. Ozone concentrations increased to 17.5 ppbv in the high Arctic region. Average UVB values were 0.26 W.m-2 and 0.14 W.m-2 in the Bering Sea and Arctic Ocean, respectively. The average SC in the Bering Sea was 4.3 M.m-1, more than twice the value measured in the Arctic Ocean, which had an average value of 1.7 M.m-1. Overall, UVB and SC values were stable in the central Arctic Ocean.
文摘Wheat (Triticum aestivum), as a kind of important economic crop cultured in the Northern China, is affected by present-day enhanced ultraviolet-B (UV-B) radiation. To get the information of the impact by UV-B radiation on it, the proteins of wheat (Jin mai NO.8) leaves, which were divided into the normal light group (CK) and UV-B radiation group (B), were extracted and ran at SDS-PAGE at different treatment days (5, 6, 7). The proteins were also analyzed by run two-dimensional gel electrophoresis (2-DE), which allowed the identification of some significantly different gel spots. The proteins spots were further verified by Matrix-Assisted Laser Desorption/lonization-time of Flight Mass Spectrometry. The results showed: 1) the enhanced UV-B affects the growth of the wheat, as the visual changes appear on the sixth day;2) the proteins expressions between the B group and the CK group were remarkably different on the sixth day;3) the proteins of wheat leaves of the sixth day were further analyzed by 2-DE revealed that twenty-one protein points were identificated between the B group and the CK group. Among these twenty-one proteins, six proteins of them were up-regulated and twelve proteins of them were down-regulated, three new proteins were expressed only in the B group. Three proteins among six proteins, which were up-regulated, were further verified as RuBisCo large subunit binding protein;SOD;Calmodulin. The result indicates wheat could improve genes encoding proteins in their leaves and protect themselves, when enhanced UV-B affects the growth of the wheat.
文摘To investigate the protective effect of epigallocatechin gallate(EGCG)on the immune func- tion of dendritic cells(DCs)after ultraviolet B irradiation(UVB)and its underlying mechanisms,the monocytes were isolated from peripheral blood and cultivated into DCs with cytokines,such as GM-CSF and IL-4.DCs were harvested after cultivation for 7 d and subjected to irradiation with different dosages of UVB.Then,200μg/ml of EGCG were added in certain groups immediately after irradiation.DCs simply treated with UVB or treated with both UVB and EGCG were co-cultured with lymphocytes,and MTF assay was used to detect the ability of DCs to stimulate proliferation of lymphocytes.Surface markers CDS0,CD86,HLA-DR and CD40 were detected by flow cytometry,and the levels of IL-10 and IL-12 secreted from DCs 24 h after cultivation were measured by ELISA.It was demonstrated that UVB irradiation could inhibit the ability of DCs to stimulate the proliferation of lymphocytes and surface expressions of CDS0,CD86,HLA-DR and CD4 on DCs in a dose-dependent manner.The inhibition rate of DCs was improved to some extent after treatment with 200μg/ml of EGCG.When the concentra- tion of EGCG exceeded 100μg/ml,the enhancing effect of EGCG on the expression of the co-stimulat- ing molecules on DCs could be demonstrated in a dose-dependent manner.UVB showed no significant influence on the secretion of IL-10 and IL-12 from DCs,while EGCG could down-regulate the secretion level of IL-12 and up-regulate that of IL-10.It is concluded that EGCG can antagonize the inhibitory effect on DCs induced by UVB irradiation.This function has some relationship with its protecting effect of the expression of the co-stimulating molecule on the surface of DCs and the secretion level of IL-10 and IL-12.
