<div style="text-align:justify;"> <span style="line-height:1.5;font-family:Verdana;"> <span style="font-family:"">C<span>arotenoids are isoprenoid pigments us...<div style="text-align:justify;"> <span style="line-height:1.5;font-family:Verdana;"> <span style="font-family:"">C<span>arotenoids are isoprenoid pigments used in food, chemical, text</span>ile, pharma<span>ceutical, and cosmetic industries. They act not only as </span>dyes and provitamins A but<span> also have antioxidants, photoprotective, anti</span>microbial properties, among others. This class of pigment can be obtained traditionally by plants or chemical synthesis, but they have some disadvantages. In recent years, search for alternative sources has been an important strategy for the carotenoid industries. Microbial synthesis is an alternative that has shown good yields, speed, <span>and reduced production costs. Hostile environments, such as the Caating</span>a dom<span>ain, represent an interesting source of microorganisms tha</span>t produce biomolecules, especially carotenoids, because of oxidative stress caused by sunlight. T<span>hus, this region has been attracting the attentio</span>n of the scientific community and industry for the use of these organisms in the production of carotenoids and applications in cosmetic products;since these compounds have interesti<span>ng antioxidant and photoprotective properties. In</span> this review, general characteristics of carotenoids, sources of production, industrial applicability, and commercialization will be discussed, as well as perspectives on the production of carotenoids from microorganisms isolated from the Caatinga and their application in anti-UV products.</span><span style="font-family:""></span> <p> <br /> </p> </span><span style="line-height:1.5;font-family:Verdana;"></span> </div> <span style="font-family:;" "=""></span>展开更多
Betanin is a member of natural pigment betacyanins family and has extensive application in the food industry as an important natural red food colorant.Its relatively inefficient production in nature however hampers ac...Betanin is a member of natural pigment betacyanins family and has extensive application in the food industry as an important natural red food colorant.Its relatively inefficient production in nature however hampers access to this phytochemicals through traditional crop-based manufacturing.Microbial bioproduction therefore represents an attractive alternative.Here,we present the construction of a Saccharomyces cerevisiae strain for betanin production.Through minimizing metabolic crosstalk,screening and modifying biosynthetic enzymes,enhancing pathway flux and optimizing fermentation conditions,a final titer of betanin of 28.7 mg/L was achieved from glucose at 25℃ in baffled shake-flask,which is the highest reported titer produced by yeast to our knowledge.This work provides a promising step towards developing synthetic yeast cell factories for de novo biosynthesis of value-added betanin and other betacyanins.展开更多
The co-crystallization of quercetin(Qur)with a flexible molecule 4-(4-pyridinyldisulfanyl)pyridine(DPDS)in different solvents and conditions was investigated,yielded five multi-component crystalline phases and charact...The co-crystallization of quercetin(Qur)with a flexible molecule 4-(4-pyridinyldisulfanyl)pyridine(DPDS)in different solvents and conditions was investigated,yielded five multi-component crystalline phases and characterized with X-ray diffractions and thermal analysis.Although the crystal system of Qur-DPDSMe OH and Qur-DPDS-Dioxane is the same,the desolvation results revealed that Qur-DPDS-Me OH transformed to Qur-DPDS when Me OH solvent molecules escape from the lattice,while Qur-DPDS-Dioxane transformed to Qur-DPDS-II through a similar process,which is same with Qur-DPDS-THF.These two cocrystal polymorphs Qur-DPDS and Qur-DPDS-II obey an enantiotropic relationship.Moreover,the formation of cocrystal solvates improves the packing efficiency of crystals.Crystal structure analysis showed that hydrogen bonds and conformations of the corresponding parent molecules play a major role in molecular assembly and crystal packing patterns,thus bring different physicochemical properties.Finally,the fluorescence spectra and quantum-chemical calculations were carried out to explore the difference in the optical-physical properties.展开更多
文摘<div style="text-align:justify;"> <span style="line-height:1.5;font-family:Verdana;"> <span style="font-family:"">C<span>arotenoids are isoprenoid pigments used in food, chemical, text</span>ile, pharma<span>ceutical, and cosmetic industries. They act not only as </span>dyes and provitamins A but<span> also have antioxidants, photoprotective, anti</span>microbial properties, among others. This class of pigment can be obtained traditionally by plants or chemical synthesis, but they have some disadvantages. In recent years, search for alternative sources has been an important strategy for the carotenoid industries. Microbial synthesis is an alternative that has shown good yields, speed, <span>and reduced production costs. Hostile environments, such as the Caating</span>a dom<span>ain, represent an interesting source of microorganisms tha</span>t produce biomolecules, especially carotenoids, because of oxidative stress caused by sunlight. T<span>hus, this region has been attracting the attentio</span>n of the scientific community and industry for the use of these organisms in the production of carotenoids and applications in cosmetic products;since these compounds have interesti<span>ng antioxidant and photoprotective properties. In</span> this review, general characteristics of carotenoids, sources of production, industrial applicability, and commercialization will be discussed, as well as perspectives on the production of carotenoids from microorganisms isolated from the Caatinga and their application in anti-UV products.</span><span style="font-family:""></span> <p> <br /> </p> </span><span style="line-height:1.5;font-family:Verdana;"></span> </div> <span style="font-family:;" "=""></span>
基金supported by the Research and Development Program in Key Areas of Guangdong Province,China (2020B0303070002).
文摘Betanin is a member of natural pigment betacyanins family and has extensive application in the food industry as an important natural red food colorant.Its relatively inefficient production in nature however hampers access to this phytochemicals through traditional crop-based manufacturing.Microbial bioproduction therefore represents an attractive alternative.Here,we present the construction of a Saccharomyces cerevisiae strain for betanin production.Through minimizing metabolic crosstalk,screening and modifying biosynthetic enzymes,enhancing pathway flux and optimizing fermentation conditions,a final titer of betanin of 28.7 mg/L was achieved from glucose at 25℃ in baffled shake-flask,which is the highest reported titer produced by yeast to our knowledge.This work provides a promising step towards developing synthetic yeast cell factories for de novo biosynthesis of value-added betanin and other betacyanins.
基金financially supported by the National Natural Science Foundation of China(Nos.22178254 and 21621004)。
文摘The co-crystallization of quercetin(Qur)with a flexible molecule 4-(4-pyridinyldisulfanyl)pyridine(DPDS)in different solvents and conditions was investigated,yielded five multi-component crystalline phases and characterized with X-ray diffractions and thermal analysis.Although the crystal system of Qur-DPDSMe OH and Qur-DPDS-Dioxane is the same,the desolvation results revealed that Qur-DPDS-Me OH transformed to Qur-DPDS when Me OH solvent molecules escape from the lattice,while Qur-DPDS-Dioxane transformed to Qur-DPDS-II through a similar process,which is same with Qur-DPDS-THF.These two cocrystal polymorphs Qur-DPDS and Qur-DPDS-II obey an enantiotropic relationship.Moreover,the formation of cocrystal solvates improves the packing efficiency of crystals.Crystal structure analysis showed that hydrogen bonds and conformations of the corresponding parent molecules play a major role in molecular assembly and crystal packing patterns,thus bring different physicochemical properties.Finally,the fluorescence spectra and quantum-chemical calculations were carried out to explore the difference in the optical-physical properties.