Discrimination of Transgenic Rice Based on Near Infrared Reflectance Spectroscopy and Partial Least Squares Regression Discriminant Analysis

Near infrared reflectance spectroscopy （NIRS）, a non-destructive measurement technique, was combined with partial least squares regression discrimiant analysis （PLS-DA） to discriminate the transgenic （TCTP and mi166） and wild type （Zhonghua 11） rice. Furthermore, rice lines transformed with protein gene （OsTCTP） and regulation gene （Osmi166） were also discriminated by the NIRS method. The performances of PLS-DA in spectral ranges of 4 000-8 000 cm-1 and 4 000-10 000 cm-1 were compared to obtain the optimal spectral range. As a result, the transgenic and wild type rice were distinguished from each other in the range of 4 000-10 000 cm-1, and the correct classification rate was 100.0% in the validation test. The transgenic rice TCTP and mi166 were also distinguished from each other in the range of 4 000-10 000 cm-1, and the correct classification rate was also 100.0%. In conclusion, NIRS combined with PLS-DA can be used for the discrimination of transgenic rice.

Determination of Protein and Starch Content in Whole Maize Kernel by Near Infrared Reflectance Spectroscopy

Using 128 bulk-kernel samples of inbred lines and hybrids, a study was conducted toinvestigate the feasibility and method of measuring protein and starch contents inintact seeds of maize by near infrared reflectance spectroscopy (NIRS). The chemometricalgorithms of partial least square (PLS) regression was used. The results indicated thatthe calibration models developed by the spectral data pretreatment of firstderivative+multivariate scattering correction within the spectral region of 10000-4000cm-1, and first derivative + straight line subtraction in 9000-4000cm-1 were thebest for protein and starch, respectively. All these models yielded coefficients ofdetermination of calibration (R2cal) above 0.97, while R2cv and R2val of cross and externalvalidation ranged from 0.92 to 0.95, respectively; however, the root of mean squareerrors of calibration, cross and external validation (RMSEE, RMSECV and RMSEP) werebelow 1(ranged 0.3-0.7),respectively. This study demonstrated that it is feasible touse NIRS as a rapid, accurate, and none-destructive technique to predict protein andstarch contents of whole kernel in the maize quality improvement program.

The Study of Using Near-infrared Diffuse Reflectance Spectroscopy Rapid Identify Wheat Drought Resistance-Ⅱ

[Objective] The aim was to build an evaluation method rapidly identifying wheat drought tolerance with near infrared diffuse reflectance spectroscopy. [Method] In the research, 36 wheat varieties in 2007-2009 were chosen and drought-tolerance degrees of wheat were graded and identified according to Winter-wheat Drought Tol- erance Evaluation Technical Standards （GB/T 21127-2007）, and harvest wheat grains underwent spectrum collection, with a full-spectrum analyzer, to establish a database. [Result] Based on qualitative analysis and full-spectrum correlation research, the coef- ficient of determination （RSQ） and cross-validation coefficient of determination （1-VR） were concluded at 0.697 5 and 0.600 2, showing near-infrared diffuse reflectance spectroscopy is of significant differences among wheat varieties and of significant or extremely significant correlation with drought-tolerance indices. [Conclusion] The re- search indicates that to evaluate drought-tolerance of wheat with near-infrared diffuse reflectance spectroscopy is a rapid and feasible way, which is simple, convenient without damages on grains, and of practical values for construction wheat drought-tol- erance evaluation index system and identification of breeding materials.

Determination of Active Components in a Natural Herb with Near Infrared Spectroscopy Based on Artificial Neural Networks

The non-linear relationships between the contents of ginsenoside Rg 1, Rb 1, Rd and Panax notoginseng saponins(PNS) in Panax notoginseng root herb and the near infrared(NIR) diffuse reflectance spectra of the herb were established by means of artificial neural networks(ANNs). Four three-layered perception feed-forward networks were trained with an error back-propagation algorithm. The significant principal components of the NIR spectral data matrix were utilized as the input of the networks. The networks architecture and parameters were selected so as to offer less prediction errors. Relative prediction errors for Rg 1, Rb 1, Rd and PNS obtained with the optimum ANN models were 8.99%, 6.54%, 8.29%, and 5.17%, respectively, which were superior to those obtained with PLSR methods. It is verified that ANN is a suitable approach to model this complex non-linearity. The developed method is fast, non-destructive and accurate and it provides a new efficient approach for determining the active components in the complex system of natural herbs.

Comparison and rapid prediction of lignocellulose and organic elements of a wide variety of rice straw based on near infrared spectroscopy

Rice straw is a major kind of biomass that can be utilized as lignocellulosic materials and renewable energy.Rapid prediction of the lignocellulose(cellulose,hemicellulose,and lignin)and organic elements(carbon,hydrogen,nitrogen,and sulfur)of rice straw would help to decipher its growth mechanisms and thereby improve its sustainable usages.In this study,364 rice straw samples featuring different rice subspecies(japonica and indica),growing seasons(early-,middle-,and late-season),and growing environments(irrigated and rainfed)were collected,the differences among which were examined by multivariate analysis of variance.Statistic results showed that the cellulose content exhibited significant differences among different growing seasons at a significant level(p<0.01),and the contents of cellulose and nitrogen had significant differences between different growing environments(p<0.01).Near infrared reflectance spectroscopy(NIRS)models for predicting the lignocellulosic and organic elements were developed based on two algorithms including partial least squares(PLS)and competitive adaptive reweighted sampling-partial least squares(CARS-PLS).Modeling results showed that most CARS-PLS models are of higher accuracy than the PLS models,possibly because the CARS-PLS models selected optimal combinations of wavenumbers,which might have enhanced the signal of chemical bonds and thereby improved the predictive efficiency.As a major contributor to the applications of rice straw,the nitrogen content was predicted precisely by the CARS-PLS model.Generally,the CARS-PLS models efficiently quantified the lignocellulose and organic elements of a wide variety of rice straw.The acceptable accuracy of the models allowed their practical applications.

Spectral feature characterization and nitrogen content prediction in soils with different particle sizes and moisture contents

The objective of this research is to analyze the influences of light source incidence angle,fiber height,moisture content,and particle size on loamy mixed soil spectra.Nitrogen(N)content calibration and cross-validation models at different moisture contents and particle sizes were obtained using partial least squares(PLS)analysis.Spectral data were collected using a spectrophotometer.Fiber height of 100 mm and light source angle at 45°were chosen to obtain the sharpest spectra without apparent scattering effect.The results show that moisture content and particle size strongly influenced the absorbance of the spectra,and a better N prediction model was obtained when the particle sizes were in the ranges of 0.5-1.0,1.0-2.0 and 2.0-5.0 mm,with the correlation coefficients(r)of 0.819,0.815 and 0.818,and standard errors of prediction(SEP)of 2.29,2.41 and 2.42 mg/kg,respectively.Poor N prediction model was obtained when the soil was kept in its natural moisture content with r of 0.575 and SEP of 3.275 mg/kg,compared to the performance of dried soil samples with r of 0.815 and SEP of 2.425 mg/kg.