Electroacupuncture at the Renzhong (DU 26) and Neiguan (PC 6) acupoints repairs the DNA of nervous tissue supplied by the middle cerebral artery in rats

This study compares the changes of DNA repair in brain tissue dominated by the middle cerebral artery after electroacupuncture at the acupoints of Renzhong （DU 26）, Neiguan （PC 6）, Quchi （LI 11） and Zusanli （ST 36） in rats. In the apurinic/apyrimidinic endonuclease DNA basic group reparative excision experiments, the apurinic/apyrimidinic endonuclease activity of brain tissue increased slightly after electroacupuncture in rats. In the DNA polymerase β （Pol β）experiments, the Pol β ac-tivity of brain tissue increased after electroacupuncture, especially at DU 26 and PC 6. In the DNA ligase experiments, the expression of DNA ligase 1 in brain tissue increased significantly after electroacupuncture. These findings demonstrate increased activity of apurinic/apyrimidinic en-donuclease, Pol β and DNA ligase 1 after electroacupuncture at DU 26 and PC 6. Also, DNA repair in brain tissue supplied by the middle cerebral artery is promoted after electroacupuncture at DU 26 and PC 6, which are more effective than the LI 11 and ST 36 acupoints .

针刺右内关穴配合康复训练治疗孤独症谱系障碍患者言语障碍的临床研究

目的基于大脑偏侧化理论观察针刺右内关穴配合康复训练治疗孤独症谱系障碍(autism spectrum disorder,ASD)患者言语障碍的临床疗效。方法将114例ASD伴言语障碍患者随机分为A组、B组和C组,每组38例。A组采用针刺右内关穴配合康复训练治疗,B组采用偏侧化治疗配合康复训练治疗,C组仅采用康复训练治疗。观察3组治疗前后孤独症儿童行为量表(autism behavior checklist,ABC)、儿童孤独症评定量表(childhood autism rating scale,CARS)、孤独症治疗评估量表(autism treatment evaluation checklist,ATEC)评分及S-S语言发育迟缓检查法中语言理解功能和表达功能评分的变化情况。结果3组治疗后ABC、CARS、ATEC评分均较同组治疗前显著降低,语言理解功能和表达功能评分均显著升高,差异均具有统计学意义(P<0.05)。A组治疗后ABC评分明显低于C组,CARS、ATEC评分明显低于B组和C组,语言理解功能评分明显高于C组,表达功能评分明显高于B组和C组,差异均具有统计学意义(P<0.05)。结论针刺右内关穴配合康复训练治疗ASD患者言语障碍疗效确切。

内关穴和足三里穴位刺激对腹腔镜结直肠癌根治术麻醉及术后胃肠功能的影响

目的 观察内关穴和足三里穴位刺激对腹腔镜结直肠癌根治术麻醉及术后胃肠功能的影响。方法 以2021年1月—2022年6月医院进行腹腔镜结直肠癌根治术的患者126例作为研究对象,通过随机数表法分为两组,每组63例,对照组术后常规镇痛干预,观察组在对照组基础上进行内关穴和足三里穴位刺激,比较两组的麻醉术后恢复、胃肠功能及免疫功能情况和不良反应发生率,观察指标包括疼痛视觉模拟评分法(Visual Analogue Scale, VAS)、拉姆齐(Ramsay)镇静评分,胃肠反应分级,肠鸣音恢复时间、肛门排气时间、进食流质饮食时间、排便时间,外周血T淋巴细胞亚群(CD^(+)3、CD^(+)4、CD^(+)8)及自然杀伤细胞(natural killer cell, NK),不良反应。结果 术后24、48 h观察组VAS评分显著低于对照组,差异均有统计学意义(P<0.05),Ramsay评分差异无统计学意义(P>0.05)。术后48 h观察组胃肠反应分级显著优于对照组,差异有统计学意义(P<0.05)。两组肠鸣音恢复时间、肛门排气时间、进食流质饮食时间比较差异有统计学意义(P<0.05),观察组均短于对照组。术后5 d两组CD^(+)3、CD^(+)4、NK水平均显著升高,且观察组高于对照组,差异有统计学意义(P<0.05),但两组CD^(+)8变化不明显,差异无统计学意义(P>0.05)。观察组不良反应发生率为9.53%,对照组不良反应发生率为14.29%,组间差异无统计学意义(P>0.05)。结论 内关穴和足三里穴位刺激有利于腹腔镜结直肠癌根治术患者麻醉及术后恢复,镇痛、镇静效果良好,胃肠功能恢复更快,提高患者免疫功能,利于术后康复。

内关穴醒脑开窍机制探析

内关为手厥阴心包经穴,有养血、调神、开窍之功,为醒脑开窍主穴。本文从内关与脑窍的联系、内关养血调神的角度探讨内关穴醒脑开窍的机制。内关通过心经连于目窍、舌窍,通过三焦经加强与耳窍的联系,与耳窍、目窍、舌窍的密切联系是其通脑窍的生理基础。内关属厥阴经穴,具备多血少气的特点;内关通于阴维脉,即阴维脉可通过内关调理津液、血液,故内关善滋阴养血。神的正常活动需以血液充盈为条件,故内关可通过调节阴血发挥养神、安神、调神的作用。现代临床中内关在心脑疾病中的应用也印证了如上机制。

内关穴注射维生素B_(6)联合穴位贴敷合治疗妊娠剧吐的效果观察

目的 探讨内关穴注射维生素B_(6)联合穴位贴敷合治疗妊娠剧吐的效果。方法 选取2022年1—10月在医院就诊的妊娠剧吐孕妇114例,按随机数字表法分为观察组与对照组,各57例。对照组给予常规妊娠呕吐干预;观察组在对照组基础上给予内关穴注射液维生素B_(6)及穴位贴敷,两组均连续干预7 d。比较两组干预前后妊娠-呕吐与恶心独特量表(PUQE)评分;比较两组干预7 d后剧吐改善情况;比较两组干预前后妊娠恶心呕吐生活质量量表(NVPQOL)评分。结果 干预后,两组PUQE评分均较干预前下降,观察组PUQE评分低于对照组,差异均有统计学意义(P<0.05);观察组呕吐次数及止吐时间均少于对照组,差异有统计学意义(P<0.05);观察组NVPQOL各项评分及总分均低于对照组,差异有统计学意义(P<0.05)。结论 内关穴注射液维生素B_(6)联合穴位贴敷能够有效减轻孕妇妊娠剧吐症状,改善患者胃肠功能,提高生活质量,值得应用。

Effect of electroacupuncture at Neiguan(PC6)at different time points on myocardial ischemia reperfusion arrhythmia in rats

OBJECTIVE:To observe the effects of electroacupuncture at Neiguan(PC6)at different time points on reperfusion arrhythmia(RA)after myocardial ischemia and reperfusion in rats,and to investigate the correlation of this protective effect with nerve growth factor(NGF),tyrosine kinase A(Trk A),tyrosine hydroxylase(TH),and norepinephrine(NE).METHODS:A total of 72 Sprague-Dawley male rats were randomly divided into six groups(n=12 rats/group):normal group(Norm),sham operation group(Sham),ischemia reperfusion group(I/R),pre-ischemic electroacupuncture group(EAI),pre-reperfusion electroacupuncture group(EAII),post-reperfusion electroacupuncture group(EAIII).The myocardial ischemia-reperfusion injury(MIRI)model was induced by occlusion of left anterior descending coronary artery for 20 min followed by reperfusion for 40 min in rats.With no intervention in the Norm group and only threading without ligation in the Sham group.Electroacupuncture pretreatment at 20 min/d for 7 d before ligation in the EAⅠgroup,20 min of electroacupuncture before reperfusion in the EAII group and 20 min of electroacupuncture after reperfusion in the EAIII group.The electrocardiogram(ECG)of each group was recorded throughout the whole process,and the success of the MIRI model was determined based on the changs of J-point and T-wave in the ECG.The arrhythmia score was used to record premature ventricular contractions,ventricular tachycardia and ventricular fibrillation during the reperfusion period to assess the reperfusion induced arrhythmias.The expression levels of NGF,Trk A,TH protein were measured by Western blot.Moreover,the expression levels of plasma and myocardial NE levels were detected by enzyme linked immunosorbent assay.RESULTS:The differences between Norm group and Sham group were not statistically significant in all indexes.Arrhythmia score,myocardial NGF,Trk A,TH,and NE expression were significantly higher in the I/R group compared with the Sham group.Arrhythmia score,myocardial NGF,Trk A,TH,and NE expression were significantly lower in each EA group compared with the I/R group.CONCLUSION:Electroacupuncture at Neiguan(PC6)at different time points can reduce the incidence and severity of reperfusion arrhythmias in rats.This protective effect is related to electroacupuncture regulating NGF,Trk A,TH,NE expression and reducing sympathetic hyperactivation.

针刺联合西药治疗心血瘀阻型胸痹的疗效观察及对血栓弹力图结果的影响

目的 观察针刺联合西药治疗心血瘀阻型胸痹的临床疗效及对血栓弹力图结果的影响。方法 选取心血瘀阻型胸痹患者共200例,随机分为对照组和试验组,每组100例。对照组给予常规西药治疗,试验组在对照组基础上针刺内关穴。采用超声心动图检测患者治疗前后的心功能指标,包括左心室射血分数(left ventricular ejection fraction, LVEF)、左室舒张末期内径(left ventricular end diastolic diameter, LVEDD)和左室收缩末期内径(left ventricular end systolic diameter, LVESD);测定6 min步行距离评估运动耐力;采用血栓弹力图仪分析计算出患者治疗前后凝血活酶生成时间(R值)、凝血时间(K值)、凝固角(Angle值)、血栓最大弹力度(MA值),并比较两组临床疗效。结果 试验组总有效率为93.0%,高于对照组的82.0%(P<0.05)。两组治疗后LVEF显著增高,LVEDD和LVESD显著降低,差异有统计学意义(P<0.05);试验组治疗后LVEF高于对照组,LVEDD和LVESD低于对照组,差异有统计学意义(P<0.05)。两组治疗后6 min步行距离增高(P<0.05),试验组治疗后6 min步行距离高于对照组(P<0.05)。两组治疗后R值和K值升高,Angle值和MA值降低,差异有统计学意义(P<0.05);试验组治疗后R值和K值高于对照组,Angle值和MA值低于对照组,差异有统计学意义(P<0.05)。结论 针刺联合西药治疗心血瘀阻型胸痹可以提高疗效,改善患者心功能,提高患者的运动耐力,改善心血瘀阻证胸痹患者血液的高凝状态,降低血栓形成的风险。

内关、太冲穴位埋线对自发性高血压前期大鼠心肌损伤和能量代谢的影响

目的:探讨内关、太冲穴位埋线对自发性高血压(SHR)前期大鼠心肌损伤和能量代谢的影响。方法:选择16只4周龄自发性高血压大鼠,随机分为埋线组与模型组(n=8),另取8只同龄的京都种Wistar(WKY)大鼠为对照组。埋线组大鼠定位内关、太冲并进行穴位埋线干预,模型组及对照组仅予以抓取固定干预,实验周期为2周。在干预前1 h及干预2、7、14 d后,测定各组大鼠收缩压(SBP)、舒张压(DBP)、平均动脉压(MAP)及心率(HR);并取心肌组织,采用HE染色比较各组大鼠左心室前壁心肌组织病理改变,电镜观察左心室前壁心肌组织线粒体的结构差异,酶联免疫吸附试验(ELISA)试剂盒测定心肌组织血管紧张素Ⅱ(AngⅡ)水平,三磷酸腺苷(ATP)检测试剂盒测定心肌组织中ATP水平。结果:干预2、7和14 d后,与模型组比较,埋线组大鼠DBP和MBP显著降低(P<0.01);干预7 d和14 d后,与模型组比较,埋线组大鼠SBP明显降低(P<0.01)。3组大鼠HR比较,差异无统计学意义(P>0.05)。模型组心肌细胞排列紊乱,心肌细胞横径和细胞间隙明显增大;与模型组比较,埋线组心肌细胞排列规则,心肌细胞横径明显减小(P<0.05),细胞间隙基本正常。在高倍透射电镜下,模型组线粒体排列紊乱,形状不规则,线粒体密度减小,埋线组较模型组有所改善。与模型组比较,埋线组左心室心肌组织中ATP水平升高,AngⅡ水平降低(P<0.05)。结论:内关、太冲穴位埋线可调节自发性高血压前期大鼠心肌能量代谢,对改善心肌损伤和降低血压水平有积极作用。

CLINICAL APPLICATION OF NEIGUAN (内关 PC 6) IN THE TREATMENT OF DISORDERS OF THE DIGESTIVE SYSTEM——THE RELATED RESEARCH PROGRESS

In the present paper, the authors review recent situations of clinical application of Neiguan (PC 6内关), etc. in the treatment of disorders of the digestive system and its related research progresses. In clinical practice, Neiguan (PC 6) is usually selected as one of the main acupoints to treat gastrointestinal reactions induced by ① nausea and vomiting, ② hiccup, ③ gastrointestinal dyskinesia, ④ gastritis, and ⑤ intra-gastric examination, cesarean section, etc. Concerning experimental studies, the effects of Neiguan (PC 6), etc. on ① gastric electrical activities, ② gastrointestinal motion, ③ gastrointestinal hormone secretion, and ④ gastric mucosal structure were observed. Results indicated that Neiguan (PC 6) is an important and effective acupoint for treatment of gastrointestinal disorders in clinic. Stimulation of Neiguan (PC 6) induced favorable regulation of both the peripheral nervous system and central nervous system, and changes of the gastrointestinal hormone secretion may contribute to its effects in treating various disorders.

NEIGUAN(PC 6) BLOCKING FOR PREVENTING NAUSEA AND VOMITING CAUSED BY SUBARACHOID MORPHINE ANALGESIA

To find a simple and an inexpensive by method to reduce postoperative nausea and vom iting abbreviated to (PONV) caused subarachoid morphine analgesia. Methods: ninty-eight gyneeco logic patients who took 0. 5 mg morphine by subarahaid space to control postoperative pain, randomly divided into group Ⅰ (n = 50) whose bilateral Neiguan were blocked by 0. 25% bupivacaine 2 ml, group Ⅱ (n= 48), who were intravenously injected ondansetron 4 mg, preoperatively and postopera tively to prevent nausea and vomiting. The numbers of the patients experienced PONV were observed for 48 hrs after operation. Result: group i had 15 patients, group Ⅱ had 14 ones, who experienced ~ (P >0. 05 ), Conclusion: Neiguan blocking can effectively prevnt PONV caused by subara choid morphine anslgesia without side effects.

Efficacy on rabbits with arrhythmia: needling acupoint of Neiguan(PC6) at shallow or deep depth, and retaining needles for 10, 20, or 30 min

OBJECTIVE:To compare the effects of Neiguan(PC6)acupuncture at different depths and retention time on arrhythmia duration,myocardial tissue morphology,mRNA expression level of L-type calcium channelα1C subunit and Ca^(2+)-Mg^(2+)-At Pase activity in tachirrhythmia model of rabbits.METHODS:The tachyarrhythmia model was made by intravenous injection of barium chloride into the ears of rabbits.A total of 56 healthy adult male New Zealand big-eared white rabbits,apply the random number table method,divided into normal control group(group A),model group(group B),shallow needling Neiguan(PC6)10 min group(group C),shallow needling Neiguan(PC6)20 min group(group D),shallow needling Neiguan(PC6)30 min group(group E),deep needling Neiguan(PC6)10 min group(group F),deep needling Neiguan(PC6)20 min group(group G),deep needling Neiguan(PC6)30 min group(group H),7 animals in each group.Electrocardiograms were used to collect the duration of arrhythmia;hematoxylin-eosin staining method was performed on myocardial tissue,RT-PCR tested the expression ofα1C subunit mRNA,and the activity of Ca^(2+)-Mg^(2+)-ATPase were quantified by phosphorus determination method.RESULTS:The duration of arrhythmia in each acupuncture treatment group was shortened to varying degrees.Compare to the model group,the tissue damage from barium chloride inducing was improved in the acupuncture group.Compared to the model group,except for group E,most treatment groups had varying degrees of improvement with significantly down-regulated L-type calcium channelα1C subunit mRNA expressions level and increased Ca^(2+)-Mg^(2+)-ATPase activity.CONCLUSIONS:The effect of acupuncture at Neiguan(PC6)with different depths and retention time can reduce the duration of arrhythmia induced by barium chloride relatively,improve the induced pathological changes,down regulate L-type calcium channelα1C subunit mRNA expressions level and increase Ca^(2+)-Mg^(2+)-ATPase activity.Both the shallow and deep tissues of Neiguan(PC6)may be involved in transmitting acupuncture information.There is an optimal induction period for shallow needling at Neiguan(PC6)to reach the best therapeutic effect.

针灸内关预处理对心肌缺血再灌注损伤兔血清NO、NOS及腺苷含量的影响

目的观察针灸内关预处理对心肌缺血再灌注损伤兔血清一氧化氮(NO)、一氧化氮合酶(NOS)及腺苷含量的影响。方法将32只新西兰大耳白兔随机分为假手术组、缺血再灌注组、电针组和艾灸组。采用结扎左冠状动脉前降支40 min再灌注60 min的方法,建立心肌缺血再灌注损伤模型。假手术组于末次捆绑结束48 h后开胸,穿线静置40 min,拔线,继续静置60 min后取材;缺血再灌注组于末次捆绑结束后48 min行缺血再灌注后取材,电针组和艾灸组分别在针刺、艾灸内关穴之后48 h再行缺血再灌注。高效液相色谱法测定血清腺苷含量,采用硝酸还原酶化学比色法检测血清NO、NOS的含量。结果缺血再灌注组血清NO、NOS及腺苷的含量较假手术组相比明显下降(P<0.05);电针组与艾灸组血清NO、NOS及腺苷的含量较缺血再灌注模型组相比均明显升高(P<0.01),而电针组与艾灸组两组间无明显差异(P>0.05)。结论电针、艾灸内关穴预处理可以提高兔血清中NO、NOS及腺苷的含量,即可以增强延迟性保护机制的细胞信号转导通路中触发物质NO、NOS、腺苷的活性和含量,表明了针灸预处理内关穴可以在48 h这一延迟时相产生针对缺血再灌注损伤心肌的保护作用。

针刺耳甲区对自发性高血压及正常大鼠血压的影响及其机理探讨

目的：探讨针刺耳甲区耳穴的降压作用及降压机制。方法：22只正常SD大鼠和15只自发高血压大鼠，氨基甲酸乙酯腹腔注射麻醉（1．0g／kg），观察迷走神经完整和颈部切断迷走神经干后耳甲区电针（75～100Hz，疏密波）和手针（约2次／S）刺激对动脉血压和心率的影响，同时记录左侧迷走神经和交感神经放电的变化。选取“内关”穴作对照。此外，在正常大鼠沿动脉插管推泣1％阿托品（0．05mg／kg）后刺激耳穴以及电刺激正常大鼠迷走神经时，记录血压和心率及神经放电指标。结果：在自发高血压大鼠和正常大鼠。电针和手针刺激耳甲区能有效降低动脉压（P〈0．001），抑制心率（P〈0．001），同时在个例动物观察到迷走神经放电增加而交感神经放电抑制。平补平泻手法针刺“内关”穴也有降压效果（P〈0．05），但与耳甲区针刺结果相比有显著性差异（P〈0．001）。正常动物的降压效果在动脉注射阿托品和迷走神经切断术后消失，而电刺激迷走神经产生类似耳针的降压和减缓心率的效果。结论：耳甲区针刺能有效降低动物的血压，这种降压作用与迷走神经结构和功能的完整性密切相关。

电针内关和神门穴对脑功能成像不同影响的观察

目的 :观察针刺内关和神门对脑功能成像的影响作用。方法 :采用功能磁共振成像技术研究电针内关和神门对不同脑区的激活状态。结果 :电针刺激内关主要引起额叶的激活 ,刺激神门主要引起颞叶的激活。结论 :针刺刺激不同的穴位 ,可以激活不同的脑区 ,电针内关和神门穴激活脑区的功能与智能有关 ,提示应用内关、神门穴治疗智能障碍有一定的客观性。

内关穴位按压配合生姜敷贴法对妊娠剧吐患者胃动素水平的影响

目的:探究内关穴位按压配合生姜敷贴法对妊娠剧烈呕吐患者胃动素水平的影响。方法:选取2017年1月至2019年3月张家港市中医医院收治的妊娠剧烈呕吐患者70例作为研究对象,按照治疗方案不同分为对照组和观察组,每组35例。对照组予以常规对症治疗,观察组在此基础上引入内关穴位按压配合生姜敷贴法进行治疗,2组患者治疗时间均为7 d。观察2组患者临床疗效以及呕吐清水、食入即吐、头晕厌食、恶心呕吐等中医症候积分;记录2组患者呕吐次数、止吐时间、住院天数、妊娠恶心呕吐生命质量量表(NVPQOL)、妊娠专用恶心呕吐量化表(PUQE),并分别于治疗前后采用放免测试法检测患者血清中胃动素,采用试带法检测2组患者尿酮体水平,同期比较2组患者上述指标的差异。结果:观察组临床疗效(94.29%)明显高于对照组(74.29%)(χ~2=5.285,P=0.022);经过治疗后,2组患者呕吐清水、食入即吐、头晕厌食、恶心呕吐等中医症候积分均出现显著降低,且观察组低于对照组(P<0.01);2组患者尿酮体均显著降低,且观察组低于对照组(P<0.01);2组患者胃动素均显著升高,且观察组高于对照组(P<0.01);2组患者PUQE、NVPQOL评分均显著降低,且观察组低于对照组(P<0.01);观察组呕吐次数、止吐时间、住院时间等均明显优于对照组(P<0.05);此外,观察组复发率为5.71%(2/35),对照组复发率为22.86%(8/35),组间差异有统计学意义(χ~2=4.200,P=0.040)。结论:内关穴位按压配合生姜敷贴法治疗妊娠剧吐疗效显著,能够有效改善机体尿酮体及胃动素水平,且可显著缩短患者呕吐时间及住院时间,减少呕吐次数,提高生命质量。

电针不同穴位对大鼠子宫平滑肌电活动的影响

目的：比较电针不同穴区对子宫肌电活动的影响。方法：共79只Wistar大鼠,包括40只正常未孕和39只已孕18-19 d的临产大鼠,用1.5%氯醛糖（50 mg/kg）和25%乌拉坦（420 mg/kg）混合液腹腔麻醉。前者随机分为对照、内关、合谷、三阴交组,每组10只;后者随机分为对照、内关和三阴交组,每组13只。腹壁切开后,于左侧中段子宫浆膜下埋藏一对针式不锈钢电极,记录子宫平滑肌肌电活动。电针取双侧“内关”“合谷”“三阴交”,刺激参数为：频率2/15 Hz,强度1-2 mA,持续20 min。结果：1）在正常非孕鼠上,与对照组比,电针“三阴交”后,子宫肌电爆发波的频率及幅度、慢波的幅度明显增加（P〈0.05）;电针“合谷”对爆发波的频率有类似“三阴交”的作用;而电针“内关”穴后,爆发波的频率及幅度和慢波的频率明显降低（P〈0.05）。与内关组比较,“三阴交”“合谷”电针期间和停电针后0-5 min大多数指标均有显著性差异（P〈0.05,0.001）。2）在妊娠后期大鼠上,与对照组比,电针“三阴交”后,子宫肌电的爆发波频率和幅度及慢波的幅度增加（P〈0.05）且持久;电针“内关”穴后,子宫肌电爆发波、慢波的频率明显减低（P〈0.05）;两组相比,爆发波的频率和幅度、慢波波幅电针期间和停针后许多时程均有显著性差异（P〈0.05）。结论：电针“三阴交”和“合谷”穴可兴奋子宫平滑肌的电活动,“三阴交”穴的作用更强,而电针“内关”穴则抑制子宫肌的电活动,不同穴位的作用具有相对特异性。

电针“内关”对心肌缺血再灌注损伤内皮素及超微结构的影响

目的 :探讨“内关”在心肌缺血再灌注损伤过程中对心肌细胞的保护作用。方法 :电针家兔左右侧“内关”穴 2 0min后 ,结扎左冠状动脉前降支 ,心电图 (ECG)监测。 4 0min后恢复灌流 ,再灌注 1小时 ,取静脉血及心肌组织 ,观察血浆内皮素 (ET)及透射电镜下组织超微结构的变化。结果 :针刺“内关”组ET含量明显降低 (P <0 0 1) ,心肌组织损伤轻微 ,肌纤维正常走向 ,少量线粒体空泡变 ,肌丝无明显坏死、溶解。结论 :电针“内关”可明显降低缺血再灌注损伤的程度 。

大鼠“内关”穴注射硝酸甘油缓解心肌缺血作用的研究

目的 :了解“内关”穴注射硝酸甘油 (NTG)防治急性心肌缺血 (MI)的作用及其机制。方法 :静脉注入垂体后叶素 (PPT)造成MI模型 ,以“内关”注入NTG缓解MI的心电图变化为指标 ,观察保留与切断G6 ～T2 脊神经前后根 (VDS)、以及毁损脊神经节 (DRG)的影响 ,并与舌下运用NTG进行比较。结果 :切断VDS对“内关”注入NTG缓解MI影响不大 ,毁损DRG后MI加重并恢复延迟 ,“内关”比舌下运用NTG防治MI的持续时间更长。结论 :“内关”注射NTG可作为防治冠心病的有效方式之一 ,“内关” DRG 心脏间存在不依赖于中枢的神经短反射通路。

针刺“内关”穴对心肌梗塞模型大鼠心脏微血管ATP酶的影响

目的:研究针刺“内关”穴对心肌梗塞后缺血区微血管ATPase活性变化的干预效果。方法:采用Mg2 + ATPase光镜酶组织化学和Na+ K+ ATPase电镜酶细胞化学方法,动态显示微血管内皮细胞的ATPase活性。结果:结扎冠脉后,血管内皮细胞出现缺血缺氧性损伤,ATPase活性降低(P <0 .0 5、0 .0 1 ) ,以2d时损伤最重;1周以后ATPase活性开始恢复,3周时仍未恢复到正常水平(P <0 .0 5 )。针刺组ATPase活性损伤较模型组为轻(P <0 .0 5、0 .0 1 ) ,其ATPase活性损伤修复较快,3周时恢复到正常水平(P >0 .0 5 )。结论:针刺“内关”穴具有改善缺血区微血管内皮细胞ATPase活性的功能。

甲状腺机能亢进症患者内关穴伏安特性研究

目的:观察甲状腺机能亢进症患者内关穴伏安特性的变化规律。方法:应用穴位伏安特性检测系统测定33例甲状腺机能亢进患者和30例健康志愿者内关穴和对照点伏安面积、惯性面积。结果:甲亢患者左右两侧内关穴增程、减程伏安面积,惯性面积显著小于对照点(P<0.05,P<0.01);健康志愿者左右内关穴增程、减程伏安面积和右侧内关穴惯性面积显著小于对照点(P<0.05,P<0.01);两侧穴位伏安特性比较显示,甲亢患者右侧内关穴惯性面积显著大于左侧(P<0.05);甲亢患者内关穴伏安特性与正常人比较无统计学意义(P>0.05)。结论:甲亢患者左右内关穴伏安特性存在失衡现象。