Objective: To investigate the prevalence ofpenicillinase-producing Neisseria gonorrhoeae (PPNG) inWuxi. Methods: Nested PCR (nPCR) was used to test for thepresence of β-lactamase DNA in urethral or cervical swabsspec...Objective: To investigate the prevalence ofpenicillinase-producing Neisseria gonorrhoeae (PPNG) inWuxi. Methods: Nested PCR (nPCR) was used to test for thepresence of β-lactamase DNA in urethral or cervical swabsspecimens from 217 patients with gonorrhea. OnenPCR-positive product was randomly selected for DNAsequencing. Results: PPNG was found in 60 of all 217 specimens(27.65%), and in 33.87% (42/124) of male and 19.35%(18/93) of female cases. The nPCR product was identical toa known clinical PPNG strain. Conclusion: The prevalence of PPNG in STD patients inWuxi is alarmingly high, and this must be taken intoconsideration for successful treatment and prevention ofgonorrhea infections.展开更多
文摘Objective: To investigate the prevalence ofpenicillinase-producing Neisseria gonorrhoeae (PPNG) inWuxi. Methods: Nested PCR (nPCR) was used to test for thepresence of β-lactamase DNA in urethral or cervical swabsspecimens from 217 patients with gonorrhea. OnenPCR-positive product was randomly selected for DNAsequencing. Results: PPNG was found in 60 of all 217 specimens(27.65%), and in 33.87% (42/124) of male and 19.35%(18/93) of female cases. The nPCR product was identical toa known clinical PPNG strain. Conclusion: The prevalence of PPNG in STD patients inWuxi is alarmingly high, and this must be taken intoconsideration for successful treatment and prevention ofgonorrhea infections.
