[ Objectives ] This study was conducted to investigate the genetic diversity of 20 lotus （Nelumbo nucifera Gaertn） samples. [ Methods ] On this optimal ISSR amplification system, 16 primers were screened with good p...[ Objectives ] This study was conducted to investigate the genetic diversity of 20 lotus （Nelumbo nucifera Gaertn） samples. [ Methods ] On this optimal ISSR amplification system, 16 primers were screened with good polymorphism, and the DNA was used to amplify the 20 plant samples. [ R^ults] The 16 primers produced 225 loci, of which 170 were polymorphic, and the polymorphic loci percentage was up to 75.56%. The genetic similarity coefficients between the 20 vari- eties ranged from 0. 577 8 to 0.951 1, which were calculated by POPGENE32. The 20 varieties by UPMGA analysis could be clustered into 2 groups, the first of which included Baiyangdian red lotus and Donggua lotus, and other varieties was included in the second group. [ Conchmions] ISSR molecular markers could be effectively used in genetic diversity and fingerprint analysis for different lotus varieties.展开更多
To achieve the dual goals of high yield and good quality with low environmental costs,slow-release fertilizer(SRF)has been widely used in lotus cultivation as new type of fertilizer instead of traditional nitrogen fer...To achieve the dual goals of high yield and good quality with low environmental costs,slow-release fertilizer(SRF)has been widely used in lotus cultivation as new type of fertilizer instead of traditional nitrogen fertilizer.However,the optimal amount of SRF and how it would promote lotus rhizome quality remain unclear.This study was designed to investigate the photosynthetic characteristics and the synthesis,accumulation,and physicochemical properties of lotus rhizome starches under six SRF levels(CK,S1,S2,S3,S4,and S5).Compared with CK(0 kg ha^(–1)),the net photosynthetic rate(P_(n))and SPAD values of leaves remained at higher levels under SRF treatment.Further research showed that SRF increased the lotus rhizome yield,the contents of amylose,amylopectin,and total starch,and the number of starch granules.Among the six SRF levels,S3(1035 kg ha^(–1))showed the greatest difference from CK and produced the highest levels.With the increasing SRF levels,the peak,hot and final viscosities decreased at first and then increased,but the setback viscosity and pasting temperature increased.In order to interpret these changes at the molecular level,the activities of key enzymes and relative expression levels of starch accumulation related genes were analyzed.Each of these parameters also increased under SRF treatment,especially under the S3 treatment.The results of this study show that SRF,especially S3(1035 kg ha^(–1)),is a suitable fertilizer option for lotus planting which can improve lotus rhizome quality by affecting starch accumulations related enzymes and genes.These results will be useful for SRF application to high-quality lotus rhizome production with low environmental costs.展开更多
莲心碱(Lien)10～100μmol/L可浓度依赖性地降低离体兔窦房结(SAN)起搏细胞慢反应动作电位幅度(APA)及零相最大上升速率((?)_(max)),延长窦性周长(SCL),并可明显拮抗Bay K 8644增大SAN起搏细胞及高K^-诱发的豚鼠乳头肌慢反应动作电位的...莲心碱(Lien)10～100μmol/L可浓度依赖性地降低离体兔窦房结(SAN)起搏细胞慢反应动作电位幅度(APA)及零相最大上升速率((?)_(max)),延长窦性周长(SCL),并可明显拮抗Bay K 8644增大SAN起搏细胞及高K^-诱发的豚鼠乳头肌慢反应动作电位的APA和((?)_(max))作用。Lien 1～100 μmol/L还可浓度依赖性地抑制犬浦氏纤维慢内向电流(I_(si)),3和100μmol/L分别使I_(si)峰值下降14和88%。结果表明Lien具有抗钙作用。展开更多
基金Supported by the Education Department Project of Fujian Province(JB11039)
文摘[ Objectives ] This study was conducted to investigate the genetic diversity of 20 lotus （Nelumbo nucifera Gaertn） samples. [ Methods ] On this optimal ISSR amplification system, 16 primers were screened with good polymorphism, and the DNA was used to amplify the 20 plant samples. [ R^ults] The 16 primers produced 225 loci, of which 170 were polymorphic, and the polymorphic loci percentage was up to 75.56%. The genetic similarity coefficients between the 20 vari- eties ranged from 0. 577 8 to 0.951 1, which were calculated by POPGENE32. The 20 varieties by UPMGA analysis could be clustered into 2 groups, the first of which included Baiyangdian red lotus and Donggua lotus, and other varieties was included in the second group. [ Conchmions] ISSR molecular markers could be effectively used in genetic diversity and fingerprint analysis for different lotus varieties.
基金financial support they received from the National Key R&D Program of China(2020YFD1000300)the earmarked fund for China Agriculture Research System(CARS-24)the HighLevel Talent Support Plan(Lv-Yang-Jin-Feng),Yangzhou,China。
文摘To achieve the dual goals of high yield and good quality with low environmental costs,slow-release fertilizer(SRF)has been widely used in lotus cultivation as new type of fertilizer instead of traditional nitrogen fertilizer.However,the optimal amount of SRF and how it would promote lotus rhizome quality remain unclear.This study was designed to investigate the photosynthetic characteristics and the synthesis,accumulation,and physicochemical properties of lotus rhizome starches under six SRF levels(CK,S1,S2,S3,S4,and S5).Compared with CK(0 kg ha^(–1)),the net photosynthetic rate(P_(n))and SPAD values of leaves remained at higher levels under SRF treatment.Further research showed that SRF increased the lotus rhizome yield,the contents of amylose,amylopectin,and total starch,and the number of starch granules.Among the six SRF levels,S3(1035 kg ha^(–1))showed the greatest difference from CK and produced the highest levels.With the increasing SRF levels,the peak,hot and final viscosities decreased at first and then increased,but the setback viscosity and pasting temperature increased.In order to interpret these changes at the molecular level,the activities of key enzymes and relative expression levels of starch accumulation related genes were analyzed.Each of these parameters also increased under SRF treatment,especially under the S3 treatment.The results of this study show that SRF,especially S3(1035 kg ha^(–1)),is a suitable fertilizer option for lotus planting which can improve lotus rhizome quality by affecting starch accumulations related enzymes and genes.These results will be useful for SRF application to high-quality lotus rhizome production with low environmental costs.
文摘莲心碱(Lien)10～100μmol/L可浓度依赖性地降低离体兔窦房结(SAN)起搏细胞慢反应动作电位幅度(APA)及零相最大上升速率((?)_(max)),延长窦性周长(SCL),并可明显拮抗Bay K 8644增大SAN起搏细胞及高K^-诱发的豚鼠乳头肌慢反应动作电位的APA和((?)_(max))作用。Lien 1～100 μmol/L还可浓度依赖性地抑制犬浦氏纤维慢内向电流(I_(si)),3和100μmol/L分别使I_(si)峰值下降14和88%。结果表明Lien具有抗钙作用。
