By applying the technique of cybridization between reticulocytes and myeloma cells, we reported that the erythroid cell cytoplasms had some regulatory effects upon the malignancy of the myeloma. Though such a method p...By applying the technique of cybridization between reticulocytes and myeloma cells, we reported that the erythroid cell cytoplasms had some regulatory effects upon the malignancy of the myeloma. Though such a method produces reliable results, to establish inducible cytogenetic mutations in myeloma cells, to select the HGPRT^- mutants and finally to select the cybrids through HAT medium, however, still need a lot of time. Recently, by using gene transfer techniques, new selectable genetic markers could be introduced into cells directly, this provides an alternative new way to make cellular hybrids. Yet up to now, the host cells for gene transfer are all the kind of cells with nuclei. Here, by展开更多
文摘By applying the technique of cybridization between reticulocytes and myeloma cells, we reported that the erythroid cell cytoplasms had some regulatory effects upon the malignancy of the myeloma. Though such a method produces reliable results, to establish inducible cytogenetic mutations in myeloma cells, to select the HGPRT^- mutants and finally to select the cybrids through HAT medium, however, still need a lot of time. Recently, by using gene transfer techniques, new selectable genetic markers could be introduced into cells directly, this provides an alternative new way to make cellular hybrids. Yet up to now, the host cells for gene transfer are all the kind of cells with nuclei. Here, by
