From the fungus Trichoderma sp., we isolated seven novel 18-residue peptaibols, neoatroviridins E-K ( 1−7 ), and six new 14-residue peptaibols, harzianins NPDG J-O ( 8−13 ). Additionally, four previously characterized...From the fungus Trichoderma sp., we isolated seven novel 18-residue peptaibols, neoatroviridins E-K ( 1−7 ), and six new 14-residue peptaibols, harzianins NPDG J-O ( 8−13 ). Additionally, four previously characterized 18-residue peptaibols neoatroviridins A-D ( 14−17 ) were also identified. The structural configurations of the newly identified peptaibols ( 1−13 ) were determined by comprehensive nuclear magnetic resonance (NMR) and high-resolution electrospray ionization tandem mass spectrometry (HR-ESI-MS/MS) data. Their absolute configurations were further determined using Marfey’s method. Notably, compounds 12 and 13 represent the first 14-residue peptaibols containing an acidic amino acid residue. In antimicrobial assessments, all 18-residue peptaibols ( 1 − 7 , 14 − 17 ) exhibited moderate inhibitory activities against Staphylococcus aureus 209P, with minimum inhibitory concentration (MIC) values ranging from 8−32 μg·mL^(−1). Moreover, compound 9 exhibited moderate inhibitory effect on Candida albicans FIM709, with a MIC value of 16 μg·mL^(−1).展开更多
基金supported by the National Key Research and Development Program of China(No.2018YFA0903200//2018YFA0903201)the National Natural Science Foundation of China(Nos.81925037 and 81973213)+6 种基金National High-level Personnel of Special Support Program(No.2017RA2259)the 111 Project of Ministry of Education of the People's Republic of China(No.B13038)Local Innovative and Research Teams Project of Guangdong Pearl River Talents Program(No.2017BT01Y036)Guangdong International Science and Technology Cooperation Base(No.2021A0505020015)Innovative and Research Teams Project of Guangdong Higher Education Institution(No.2021KCXTD001)K.C.Wong Education Foundation(No.Guo-Dong Chen,2021,China)the Science and Technology Planning Project of Guangzhou(No.202201020048)。
文摘From the fungus Trichoderma sp., we isolated seven novel 18-residue peptaibols, neoatroviridins E-K ( 1−7 ), and six new 14-residue peptaibols, harzianins NPDG J-O ( 8−13 ). Additionally, four previously characterized 18-residue peptaibols neoatroviridins A-D ( 14−17 ) were also identified. The structural configurations of the newly identified peptaibols ( 1−13 ) were determined by comprehensive nuclear magnetic resonance (NMR) and high-resolution electrospray ionization tandem mass spectrometry (HR-ESI-MS/MS) data. Their absolute configurations were further determined using Marfey’s method. Notably, compounds 12 and 13 represent the first 14-residue peptaibols containing an acidic amino acid residue. In antimicrobial assessments, all 18-residue peptaibols ( 1 − 7 , 14 − 17 ) exhibited moderate inhibitory activities against Staphylococcus aureus 209P, with minimum inhibitory concentration (MIC) values ranging from 8−32 μg·mL^(−1). Moreover, compound 9 exhibited moderate inhibitory effect on Candida albicans FIM709, with a MIC value of 16 μg·mL^(−1).
