Cankered, dying seedlings of Juglans regia were observed in Shaanxi province in the northwest region of China. Neofusicoccum parvum was isolated from these cankered tissues, with the identification based on mor- pholo...Cankered, dying seedlings of Juglans regia were observed in Shaanxi province in the northwest region of China. Neofusicoccum parvum was isolated from these cankered tissues, with the identification based on mor- phology and an ITS-nrDNA sequence. In order to demonstrate how cultures of N. parvum could cause the expected symptoms, artificial infection, using these isolates and re-isolation of the pathogen, was used. This is the first report on this taxon as a walnut canker pathogen in China.展开更多
Neofusicoccum parvum,an endophytic fungus isolated from Elaeocarpus serratus(Ceylon Olive;family Elaeocarpaceae),was grown for 3 weeks in potato dextrose broth.Chromatographic separation of the ethyl acetate extracts ...Neofusicoccum parvum,an endophytic fungus isolated from Elaeocarpus serratus(Ceylon Olive;family Elaeocarpaceae),was grown for 3 weeks in potato dextrose broth.Chromatographic separation of the ethyl acetate extracts from the culture filtrate and mycelium over silica gel,Sephadex LH-20 and preparative thin layer chromatography furnished(R)-7-hydroxymellein(1),(3R,4R)-4-hydroxymellein(2),(3R,4S)-4-hydroxymellein(3),(R)-5-hydroxymellein(4),(R)-mellein(5),(3R,4R)-4,7-dihydroxymellein(6),(6R,7S)-dia-asperlin(7),CJ-14445(8)and 13,14,15,16-tetranorlabd-7-ene-19,6β:12,17-diolide(9).The structures of known compounds 1–9 were determined by spectroscopic methods and comparison with reported data.This is the first report of the isolation of an endophytic fungus from E.serratus,and the isolation of compounds 1,4,6,8 and 9 from N.parvum.It is important to note that compounds 1–7 are small molecules with an oxygen heterocyclic ring system.These compounds can be used as starting materials in the synthesis of pharmaceutically important large molecules with oxygen heterocyles.展开更多
[Objectives]The study was to identify the causal agent of leaf spots of Camellia drupifera in Hainan Province,China.[Methods]Phylogenetic analyses,spore morphology and pathogenicity tests were adopted to identify the ...[Objectives]The study was to identify the causal agent of leaf spots of Camellia drupifera in Hainan Province,China.[Methods]Phylogenetic analyses,spore morphology and pathogenicity tests were adopted to identify the pathogen.[Results]The fungus was identified as Neofusicoccum parvum.The colonies were initially pale to white on PDA,with diffuse yellow pigment around the colonies in the agar after 5 d.After 10 d,the aerial mycelium became gray,and the substrate mycelium became black.Thirty days later,a large number of conidia were generated on OMA.Conidia were hyaline,nonseptate,and fusiform.The mean size of 100 conidia was(13.6-25.4)×(6.2-10.3)μm;the mean length/width ratio was(2.5±0.3)μm.[Conclusions]N.parvum was the causal agent of leaf spots of C.drupifera observed in Hainan Province,China.展开更多
基金supported by the program for tacking key problem of Shaanxi agricultural scientific and technological extent(2015NY124)Project NSFC(31270690)+1 种基金Project PCSIRT(NO.IRT1035)special funding for basic S&T work of Ministry of Science and Technology(2009FY210100)of China
文摘Cankered, dying seedlings of Juglans regia were observed in Shaanxi province in the northwest region of China. Neofusicoccum parvum was isolated from these cankered tissues, with the identification based on mor- phology and an ITS-nrDNA sequence. In order to demonstrate how cultures of N. parvum could cause the expected symptoms, artificial infection, using these isolates and re-isolation of the pathogen, was used. This is the first report on this taxon as a walnut canker pathogen in China.
文摘Neofusicoccum parvum,an endophytic fungus isolated from Elaeocarpus serratus(Ceylon Olive;family Elaeocarpaceae),was grown for 3 weeks in potato dextrose broth.Chromatographic separation of the ethyl acetate extracts from the culture filtrate and mycelium over silica gel,Sephadex LH-20 and preparative thin layer chromatography furnished(R)-7-hydroxymellein(1),(3R,4R)-4-hydroxymellein(2),(3R,4S)-4-hydroxymellein(3),(R)-5-hydroxymellein(4),(R)-mellein(5),(3R,4R)-4,7-dihydroxymellein(6),(6R,7S)-dia-asperlin(7),CJ-14445(8)and 13,14,15,16-tetranorlabd-7-ene-19,6β:12,17-diolide(9).The structures of known compounds 1–9 were determined by spectroscopic methods and comparison with reported data.This is the first report of the isolation of an endophytic fungus from E.serratus,and the isolation of compounds 1,4,6,8 and 9 from N.parvum.It is important to note that compounds 1–7 are small molecules with an oxygen heterocyclic ring system.These compounds can be used as starting materials in the synthesis of pharmaceutically important large molecules with oxygen heterocyles.
基金Hainan Provincial Natural Science Foundation of China(319MS081)Central Finance Forestry Science and Technology Extension Project(Qiong[2020]TG06).
文摘[Objectives]The study was to identify the causal agent of leaf spots of Camellia drupifera in Hainan Province,China.[Methods]Phylogenetic analyses,spore morphology and pathogenicity tests were adopted to identify the pathogen.[Results]The fungus was identified as Neofusicoccum parvum.The colonies were initially pale to white on PDA,with diffuse yellow pigment around the colonies in the agar after 5 d.After 10 d,the aerial mycelium became gray,and the substrate mycelium became black.Thirty days later,a large number of conidia were generated on OMA.Conidia were hyaline,nonseptate,and fusiform.The mean size of 100 conidia was(13.6-25.4)×(6.2-10.3)μm;the mean length/width ratio was(2.5±0.3)μm.[Conclusions]N.parvum was the causal agent of leaf spots of C.drupifera observed in Hainan Province,China.
