选择含有内生真菌的鹅观草,采集其成熟种子,经过南京的6—9月室温处理后冷藏。经过发芽、育苗以及田间栽培试验,调查并分析这些植物的长势、分蘖数、株高、茎宽、叶宽的变化,最后检测这些鹅观草植株中内生真菌的有无。结果表明:含内生真...选择含有内生真菌的鹅观草,采集其成熟种子,经过南京的6—9月室温处理后冷藏。经过发芽、育苗以及田间栽培试验,调查并分析这些植物的长势、分蘖数、株高、茎宽、叶宽的变化,最后检测这些鹅观草植株中内生真菌的有无。结果表明:含内生真菌(EI)的鹅观草植株群苗期生长比较旺盛、分蘖比较多,但在成熟期株高、茎秆直径及叶宽与不含菌植株无显著差异。从EI鹅观草植株中随机分离获得4个内生真菌菌株,其形态学特征和系统发育学特征显示,分离菌株均为Neotyphodium sinicum Wang,Ji et Kang。本研究首次证明了在田间条件下内生真菌N.sinicum能促进其宿主鹅观草的苗期生长和分蘖,揭示了禾本科植物/内生真菌共生体可作为麦类作物育种利用的可能性。展开更多
Dear Editor,Achnatherum inebrians （Hance） Keng （drunken horse grass, DHG） is a toxic perennial bunchgrass, which is so-named because it is associated with the narcosis of livestock which graze on native grasslands...Dear Editor,Achnatherum inebrians （Hance） Keng （drunken horse grass, DHG） is a toxic perennial bunchgrass, which is so-named because it is associated with the narcosis of livestock which graze on native grasslands in the northwest of China [1]. DHG is distributed mainly throughout the harsh conditions of alpine or sub-alpine grasslands, and this species is usual- ly infected by the fungal endophyte Neotyphodium gan- suense [2], which apparently enhances its resistance to the abiotic and biotic stress [3].展开更多
Various cadmium （Cd） concentrations （0, 50, 100, 200 and 300 μmol L-1） affected Elymus dahuricus seed germination, seed- ling growth, antioxidative enzymes activities （AEA）, and amounts of malondialdehyde （MDA...Various cadmium （Cd） concentrations （0, 50, 100, 200 and 300 μmol L-1） affected Elymus dahuricus seed germination, seed- ling growth, antioxidative enzymes activities （AEA）, and amounts of malondialdehyde （MDA） and proline present. These in- fluences were determined for separate E. dahuricus cohorts known to be either infected （E＋） or non-infected （E-） by a Neo- typhodium endophyte. Under high Cd concentrations （100, 200 and 300 lamol L-l）, E＋ specimens showed a significantly （P〈0.05） higher germination rate and index, as well as higher values for shoot length, root length and dry biomass. However, the germination rate and index, root length and dry weight did not show a significant （P〈0.05） difference under the low Cd concentrations （0 and 50 μmol L^-1）. AEA and proline content increased, as did MDA content, in the E＋ （vs. E-） specimens under high Cd concentrations. There was no significant （P〉0.05） difference under low Cd concentrations. Endophyte infection was concluded to be of benefit to E. dahuricus exposed to high Cd concentrations.展开更多
文摘选择含有内生真菌的鹅观草,采集其成熟种子,经过南京的6—9月室温处理后冷藏。经过发芽、育苗以及田间栽培试验,调查并分析这些植物的长势、分蘖数、株高、茎宽、叶宽的变化,最后检测这些鹅观草植株中内生真菌的有无。结果表明:含内生真菌(EI)的鹅观草植株群苗期生长比较旺盛、分蘖比较多,但在成熟期株高、茎秆直径及叶宽与不含菌植株无显著差异。从EI鹅观草植株中随机分离获得4个内生真菌菌株,其形态学特征和系统发育学特征显示,分离菌株均为Neotyphodium sinicum Wang,Ji et Kang。本研究首次证明了在田间条件下内生真菌N.sinicum能促进其宿主鹅观草的苗期生长和分蘖,揭示了禾本科植物/内生真菌共生体可作为麦类作物育种利用的可能性。
基金supported by the National Basic Research Program of China(2014CB138702)the Fundamental Research Funds for the Central Universities(Lzujbky-2014-75)the Program for Changjiang Scholars and Innovative Research Team in University(IRT13019)
文摘Dear Editor,Achnatherum inebrians （Hance） Keng （drunken horse grass, DHG） is a toxic perennial bunchgrass, which is so-named because it is associated with the narcosis of livestock which graze on native grasslands in the northwest of China [1]. DHG is distributed mainly throughout the harsh conditions of alpine or sub-alpine grasslands, and this species is usual- ly infected by the fungal endophyte Neotyphodium gan- suense [2], which apparently enhances its resistance to the abiotic and biotic stress [3].
基金supported by the National Basic Research Program of China(Grant No.2007CB108902)the National Natural Science Foundation of China(Grant No.30771531)+2 种基金the Program for a New Century of Excellent Talents in the University(Grant No.NCET-08-0256)Fundamental Research Funds for the Central Universities(Grant No.lzujky-2012-k01)the Scholarship Award for Excellent Doctoral Student granted by Ministry of Education(Grant No.224000-860008)
文摘Various cadmium （Cd） concentrations （0, 50, 100, 200 and 300 μmol L-1） affected Elymus dahuricus seed germination, seed- ling growth, antioxidative enzymes activities （AEA）, and amounts of malondialdehyde （MDA） and proline present. These in- fluences were determined for separate E. dahuricus cohorts known to be either infected （E＋） or non-infected （E-） by a Neo- typhodium endophyte. Under high Cd concentrations （100, 200 and 300 lamol L-l）, E＋ specimens showed a significantly （P〈0.05） higher germination rate and index, as well as higher values for shoot length, root length and dry biomass. However, the germination rate and index, root length and dry weight did not show a significant （P〈0.05） difference under the low Cd concentrations （0 and 50 μmol L^-1）. AEA and proline content increased, as did MDA content, in the E＋ （vs. E-） specimens under high Cd concentrations. There was no significant （P〉0.05） difference under low Cd concentrations. Endophyte infection was concluded to be of benefit to E. dahuricus exposed to high Cd concentrations.
