Netrin-1 and its receptors play crucial roles in inducing axonal growth and neuronal migration during neuronal development.Their profound impacts then extend into adulthood to encompass the maintenance of neuronal sur...Netrin-1 and its receptors play crucial roles in inducing axonal growth and neuronal migration during neuronal development.Their profound impacts then extend into adulthood to encompass the maintenance of neuronal survival and synaptic function.Increasing amounts of evidence highlight several key points:(1)Diminished Netrin-1 levels exacerbate pathological progression in animal models of Alzheimer’s disease and Parkinson’s disease,and potentially,similar alterations occur in humans.(2)Genetic mutations of Netrin-1 receptors increase an individuals’susceptibility to neurodegenerative disorders.(3)Therapeutic approaches targeting Netrin-1 and its receptors offer the benefits of enhancing memory and motor function.(4)Netrin-1 and its receptors show genetic and epigenetic alterations in a variety of cancers.These findings provide compelling evidence that Netrin-1 and its receptors are crucial targets in neurodegenerative diseases.Through a comprehensive review of Netrin-1 signaling pathways,our objective is to uncover potential therapeutic avenues for neurodegenerative disorders.展开更多
研究Netrin-1与脑出血的相关性。方法 体内实验中,将随机选取的42只大鼠分为假手术组和脑出血组,脑出血组又根据时间点分为6h、12h、24h、48h、72h、1周6组。在相应时间点处死,每组大鼠取血肿脑组织切片,使用Western blot 、双重免疫荧...研究Netrin-1与脑出血的相关性。方法 体内实验中,将随机选取的42只大鼠分为假手术组和脑出血组,脑出血组又根据时间点分为6h、12h、24h、48h、72h、1周6组。在相应时间点处死,每组大鼠取血肿脑组织切片,使用Western blot 、双重免疫荧光技术分析其Netrin-1表达水平,尾静脉采血,取血清,使用酶联免疫技术检测Netrin-1水平,体外实验部分取人类脑血管内皮细胞根据体内实验的7组,使用Western blot检测内皮细胞中Netrin-1水平。结果 体内实验结果显示,大鼠脑出血后脑组织中Netrin-1表达增加,脑出血后24hNetrin-1升高显著(P<0.05)。体外人类血管内皮实验结果显示Netrin-1蛋白表达升高维持,血清中Netrin-1升高速度慢于脑组织内皮细胞。结论 脑出血后Netrin-1表达升高,脑出血后24h升至最高。展开更多
基金supported by the National Natural Science Foundation of China(Youth Science Fund Project),No.81901292(to GC)the National Key Research and Development Program of China,No.2021YFC2502100(to GC)the National Natural Science Foundation of China,No.82071183(to ZZ).
文摘Netrin-1 and its receptors play crucial roles in inducing axonal growth and neuronal migration during neuronal development.Their profound impacts then extend into adulthood to encompass the maintenance of neuronal survival and synaptic function.Increasing amounts of evidence highlight several key points:(1)Diminished Netrin-1 levels exacerbate pathological progression in animal models of Alzheimer’s disease and Parkinson’s disease,and potentially,similar alterations occur in humans.(2)Genetic mutations of Netrin-1 receptors increase an individuals’susceptibility to neurodegenerative disorders.(3)Therapeutic approaches targeting Netrin-1 and its receptors offer the benefits of enhancing memory and motor function.(4)Netrin-1 and its receptors show genetic and epigenetic alterations in a variety of cancers.These findings provide compelling evidence that Netrin-1 and its receptors are crucial targets in neurodegenerative diseases.Through a comprehensive review of Netrin-1 signaling pathways,our objective is to uncover potential therapeutic avenues for neurodegenerative disorders.
