Diseases and disorders of the central nervous system often require significant interventions to restore lost function due to their com- plexity. Examples of such disorders include Parkinson＇s disease, Alzheimer＇s di...Diseases and disorders of the central nervous system often require significant interventions to restore lost function due to their com- plexity. Examples of such disorders include Parkinson＇s disease, Alzheimer＇s disease, multiple sclerosis, traumatic brain injury, and spinal cord in）ury. These diseases and disorders result trom healthy cells being destroyed, which in turn causes dysfunction in the cen- tral nervous system, The death of these cells can trigger a cascade of events that affect the rest of the body, causing symptoms that become progressively worse over time. Developing strategies for repairing the damage to the central nervous system remains chal- lenging, in part due to its inability to regenerate.展开更多
This study sought to elucidate the changes of nuclear factor kappa B （NF-KB） expression and locomotor function of hind limb after subdural injection of BMS-345541 was applied in rats with acute spinal cord injury. T...This study sought to elucidate the changes of nuclear factor kappa B （NF-KB） expression and locomotor function of hind limb after subdural injection of BMS-345541 was applied in rats with acute spinal cord injury. The results indicated that BMS-345541 treatment reduced the expression of NF-kB at 24 hours after injury, compared with normal saline-treated rats. This treatment also led to a significant improvement in locomotor functional recovery at 14 days after injury. Overall, the findings demonstrated that BMS-345541 significantly ameliorated spinal cord injury-induced hind limb dysfunction by inhibiting the expression of NF-kB after spinal cord injury.展开更多
Previous studies have shown that proliferation of endogenous neural precursor cells cannot alone compensate for the damage to neurons and axons. From the perspective of neural plastici- ty, we observed the effects of ...Previous studies have shown that proliferation of endogenous neural precursor cells cannot alone compensate for the damage to neurons and axons. From the perspective of neural plastici- ty, we observed the effects of functional electrical stimulation treatment on endogenous neural precursor cell proliferation and expression of basic fibroblast growth factor and epidermal growth factor in the rat brain on the infarct side. Functional electrical stimulation was performed in rat models of acute middle cerebral artery occlusion. Simultaneously, we set up a placebo stimulation group and a sham-operated group. Immunohistochemical staining showed that, at 7 and 14 days, compared with the placebo group, the numbers of nestin （a neural precursor cell marker）-positive cells in the subgranular zone and subventricular zone were increased in the functional electrical stimulation treatment group. Western blot assays and reverse-transcription PCR showed that total protein levels and gene expression of epidermal growth factor and basic fibroblast growth factor were also upregulated on the infarct side. Prehensile traction test results showed that, at 14 days, prehension function of rats in the functional electrical stimulation group was significantly better than in the placebo group. These results suggest that functional electrical stimulation can promote endogenous neural precursor cell proliferation in the brains of acute cerebral infarction rats, enhance expression of basic fibroblast growth factor and epidermal growth factor, and improve the motor function of rats.展开更多
Using electroacupuncture and moxibustion to treat peripheral nerve injury is highly efficient with low side effects. However, the electroacupuncture-and moxibustion-based mechanisms underlying nerve repair are still u...Using electroacupuncture and moxibustion to treat peripheral nerve injury is highly efficient with low side effects. However, the electroacupuncture-and moxibustion-based mechanisms underlying nerve repair are still unclear. Here, in vivo and in vitro experiments uncovered one mechanism through which electroacupuncture and moxibustion affect regeneration after peripheral nerve injury. We first established rat models of sciatic nerve injury using neurotomy. Rats were treated with electroacupuncture or moxibustion at acupoints Huantiao （GB30） and Zusanli （ST36）. Each treatment lasted 15 minutes, and treatments were given six times a week for 4 consecutive weeks. Behavioral testing was used to determine the sciatic functional index. We used electrophysiological detection to measure sciatic nerve conduction velocity and performed hematoxylin-eosin staining to determine any changes in the gastrocnemius muscle. We used immunohistochemistry to observe changes in the expression of S100—a specific marker for Schwann cells—and an enzyme-linked immunosorbent assay to detect serum level of nerve growth factor. Results showed that compared with the model-only group, sciatic functional index, recovery rate of conduction velocity, diameter recovery of the gastrocnemius muscle fibers, number of S100-immunoreactive cells,and level of nerve growth factor were greater in the electroacupuncture and moxibustion groups. The efficacy did not differ between treatment groups. The serum from treated rats was collected and used to stimulate Schwann cells cultured in vitro. Results showed that the viability of Schwann cells was much higher in the treatment groups than in the model group at 3 and 5 days after treatment. These findings indicate that electroacupuncture and moxibustion promoted nerve regeneration and functional recovery; its mechanism might be associated with the enhancement of Schwann cell proliferation and upregulation of nerve growth factor.展开更多
目的观察醒脑开窍针法联合乌灵胶囊治疗卒中后抑郁的临床疗效及对患者自噬相关蛋白水平的影响。方法将132例卒中后抑郁患者按随机数字表法分为对照组(66例)和联合组(66例)。两组均接受常规治疗,对照组另予口服乌灵胶囊治疗,联合组另予...目的观察醒脑开窍针法联合乌灵胶囊治疗卒中后抑郁的临床疗效及对患者自噬相关蛋白水平的影响。方法将132例卒中后抑郁患者按随机数字表法分为对照组(66例)和联合组(66例)。两组均接受常规治疗,对照组另予口服乌灵胶囊治疗,联合组另予醒脑开窍针法联合口服乌灵胶囊治疗。比较两组临床疗效和不良反应发生率。观察治疗前后两组美国国立卫生研究院卒中量表(National Institute of Health stroke scale,NIHSS)、汉密顿抑郁量表(Hamilton depression scale,HAMD)和日常生活能力量表(activity of daily living,ADL)的评分变化。比较两组治疗前后血清炎症因子[白介素-1β(interleukin-1β,IL-1β)、白介素-6(interleukin-6,IL-6)、肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)和核因子-κB(nuclear factor kappa-B,NF-κB)]的水平以及自噬相关蛋白[微管相关蛋白1轻链3-Ⅱ(microtubule-associated protein light chain 3-Ⅱ,LC3-Ⅱ)、自噬底物蛋白(autophagy substrate protein,p62)和自噬相关蛋白1(autophagy related protein 1,Beclin1)]的水平。结果联合组总有效率高于对照组(P<0.05)。与治疗前比较,两组治疗后HAMD和NIHSS评分均降低(P<0.05),ADL评分均升高(P<0.05);联合组治疗后HAMD和NIHSS评分低于对照组(P<0.05),ADL评分高于对照组(P<0.05)。与治疗前比较,两组治疗后血清IL-1β、IL-6、TNF-α和NF-κB水平均降低(P<0.05);联合组治疗后上述血清炎症因子水平均低于对照组(P<0.05)。与治疗前比较,两组治疗后血清Beclin1和LC3-Ⅱ水平均降低(P<0.05),血清p62水平均升高(P<0.05);联合组治疗后血清Beclin1和LC3-Ⅱ水平低于对照组(P<0.05),血清p62水平高于对照组(P<0.05)。两组不良反应发生率比较,差异无统计学意义(P>0.05)。结论在常规治疗基础上,醒脑开窍针法联合乌灵胶囊治疗卒中后抑郁可以改善患者抑郁程度和神经功能、提高日常生活能力、降低炎症反应及减轻自噬程度,疗效优于单纯乌灵胶囊治疗。展开更多
基金supported by grants from the Canada Research Chairs programthe NSERC Engage and Engage Plus program
文摘Diseases and disorders of the central nervous system often require significant interventions to restore lost function due to their com- plexity. Examples of such disorders include Parkinson＇s disease, Alzheimer＇s disease, multiple sclerosis, traumatic brain injury, and spinal cord in）ury. These diseases and disorders result trom healthy cells being destroyed, which in turn causes dysfunction in the cen- tral nervous system, The death of these cells can trigger a cascade of events that affect the rest of the body, causing symptoms that become progressively worse over time. Developing strategies for repairing the damage to the central nervous system remains chal- lenging, in part due to its inability to regenerate.
文摘This study sought to elucidate the changes of nuclear factor kappa B （NF-KB） expression and locomotor function of hind limb after subdural injection of BMS-345541 was applied in rats with acute spinal cord injury. The results indicated that BMS-345541 treatment reduced the expression of NF-kB at 24 hours after injury, compared with normal saline-treated rats. This treatment also led to a significant improvement in locomotor functional recovery at 14 days after injury. Overall, the findings demonstrated that BMS-345541 significantly ameliorated spinal cord injury-induced hind limb dysfunction by inhibiting the expression of NF-kB after spinal cord injury.
基金the National Natural Science Foundation of China,grants No.30772304,30973166,and 81171863
文摘Previous studies have shown that proliferation of endogenous neural precursor cells cannot alone compensate for the damage to neurons and axons. From the perspective of neural plastici- ty, we observed the effects of functional electrical stimulation treatment on endogenous neural precursor cell proliferation and expression of basic fibroblast growth factor and epidermal growth factor in the rat brain on the infarct side. Functional electrical stimulation was performed in rat models of acute middle cerebral artery occlusion. Simultaneously, we set up a placebo stimulation group and a sham-operated group. Immunohistochemical staining showed that, at 7 and 14 days, compared with the placebo group, the numbers of nestin （a neural precursor cell marker）-positive cells in the subgranular zone and subventricular zone were increased in the functional electrical stimulation treatment group. Western blot assays and reverse-transcription PCR showed that total protein levels and gene expression of epidermal growth factor and basic fibroblast growth factor were also upregulated on the infarct side. Prehensile traction test results showed that, at 14 days, prehension function of rats in the functional electrical stimulation group was significantly better than in the placebo group. These results suggest that functional electrical stimulation can promote endogenous neural precursor cell proliferation in the brains of acute cerebral infarction rats, enhance expression of basic fibroblast growth factor and epidermal growth factor, and improve the motor function of rats.
基金supported by the National Natural Science Foundation of China,No.81373754,81102670
文摘Using electroacupuncture and moxibustion to treat peripheral nerve injury is highly efficient with low side effects. However, the electroacupuncture-and moxibustion-based mechanisms underlying nerve repair are still unclear. Here, in vivo and in vitro experiments uncovered one mechanism through which electroacupuncture and moxibustion affect regeneration after peripheral nerve injury. We first established rat models of sciatic nerve injury using neurotomy. Rats were treated with electroacupuncture or moxibustion at acupoints Huantiao （GB30） and Zusanli （ST36）. Each treatment lasted 15 minutes, and treatments were given six times a week for 4 consecutive weeks. Behavioral testing was used to determine the sciatic functional index. We used electrophysiological detection to measure sciatic nerve conduction velocity and performed hematoxylin-eosin staining to determine any changes in the gastrocnemius muscle. We used immunohistochemistry to observe changes in the expression of S100—a specific marker for Schwann cells—and an enzyme-linked immunosorbent assay to detect serum level of nerve growth factor. Results showed that compared with the model-only group, sciatic functional index, recovery rate of conduction velocity, diameter recovery of the gastrocnemius muscle fibers, number of S100-immunoreactive cells,and level of nerve growth factor were greater in the electroacupuncture and moxibustion groups. The efficacy did not differ between treatment groups. The serum from treated rats was collected and used to stimulate Schwann cells cultured in vitro. Results showed that the viability of Schwann cells was much higher in the treatment groups than in the model group at 3 and 5 days after treatment. These findings indicate that electroacupuncture and moxibustion promoted nerve regeneration and functional recovery; its mechanism might be associated with the enhancement of Schwann cell proliferation and upregulation of nerve growth factor.
文摘目的观察醒脑开窍针法联合乌灵胶囊治疗卒中后抑郁的临床疗效及对患者自噬相关蛋白水平的影响。方法将132例卒中后抑郁患者按随机数字表法分为对照组(66例)和联合组(66例)。两组均接受常规治疗,对照组另予口服乌灵胶囊治疗,联合组另予醒脑开窍针法联合口服乌灵胶囊治疗。比较两组临床疗效和不良反应发生率。观察治疗前后两组美国国立卫生研究院卒中量表(National Institute of Health stroke scale,NIHSS)、汉密顿抑郁量表(Hamilton depression scale,HAMD)和日常生活能力量表(activity of daily living,ADL)的评分变化。比较两组治疗前后血清炎症因子[白介素-1β(interleukin-1β,IL-1β)、白介素-6(interleukin-6,IL-6)、肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)和核因子-κB(nuclear factor kappa-B,NF-κB)]的水平以及自噬相关蛋白[微管相关蛋白1轻链3-Ⅱ(microtubule-associated protein light chain 3-Ⅱ,LC3-Ⅱ)、自噬底物蛋白(autophagy substrate protein,p62)和自噬相关蛋白1(autophagy related protein 1,Beclin1)]的水平。结果联合组总有效率高于对照组(P<0.05)。与治疗前比较,两组治疗后HAMD和NIHSS评分均降低(P<0.05),ADL评分均升高(P<0.05);联合组治疗后HAMD和NIHSS评分低于对照组(P<0.05),ADL评分高于对照组(P<0.05)。与治疗前比较,两组治疗后血清IL-1β、IL-6、TNF-α和NF-κB水平均降低(P<0.05);联合组治疗后上述血清炎症因子水平均低于对照组(P<0.05)。与治疗前比较,两组治疗后血清Beclin1和LC3-Ⅱ水平均降低(P<0.05),血清p62水平均升高(P<0.05);联合组治疗后血清Beclin1和LC3-Ⅱ水平低于对照组(P<0.05),血清p62水平高于对照组(P<0.05)。两组不良反应发生率比较,差异无统计学意义(P>0.05)。结论在常规治疗基础上,醒脑开窍针法联合乌灵胶囊治疗卒中后抑郁可以改善患者抑郁程度和神经功能、提高日常生活能力、降低炎症反应及减轻自噬程度,疗效优于单纯乌灵胶囊治疗。
