Traumatic brain injury（TBI） is a leading cause of death and disability in individuals worldwide.Producing a clinically relevant TBI model in small-sized animals remains fairly challenging.For good screening of poten...Traumatic brain injury（TBI） is a leading cause of death and disability in individuals worldwide.Producing a clinically relevant TBI model in small-sized animals remains fairly challenging.For good screening of potential therapeutics,which are effective in the treatment of TBI,animal models of TBI should be established and standardized.In this study,we established mouse models of closed head injury using the Shohami weight-drop method with some modifications concerning cognitive deficiency assessment and provided a detailed description of the severe TBI animal model.We found that 250 g falling weight from 2 cm height produced severe closed head injury in C57BL/6 male mice.Cognitive disorders in mice with severe closed head injury could be detected using passive avoidance test on day 7 after injury.Findings from this study indicate that weight-drop injury animal models are suitable for further screening of brain neuroprotectants and potentially are similar to those seen in human TBI.展开更多
Modeling brain disorders has always been one of the key tasks in neurobiological studies. A wide range of organisms including worms, fruit ？ies, zebra？sh, and rodents have been used for modeling brain disorders. How...Modeling brain disorders has always been one of the key tasks in neurobiological studies. A wide range of organisms including worms, fruit ？ies, zebra？sh, and rodents have been used for modeling brain disorders. However,whether complicated neurological and psychiatric symptoms can be faithfully mimicked in animals is still debatable.In this review, we discuss key ？ndings using non-human primates to address the neural mechanisms underlying stress and anxiety behaviors, as well as technical advances for establishing genetically-engineered non-human primate models of autism spectrum disorders and other disorders.Considering the close evolutionary connections and similarity of brain structures between non-human primates and humans, together with the rapid progress in genome-editing technology, non-human primates will be indispensable for pathophysiological studies and exploring potential therapeutic methods for treating brain disorders.展开更多
基金supported by a grant from the Ministry of Higher Education of Malaysia,No.RAGS/2013/UPNM/SKK/01/2
文摘Traumatic brain injury（TBI） is a leading cause of death and disability in individuals worldwide.Producing a clinically relevant TBI model in small-sized animals remains fairly challenging.For good screening of potential therapeutics,which are effective in the treatment of TBI,animal models of TBI should be established and standardized.In this study,we established mouse models of closed head injury using the Shohami weight-drop method with some modifications concerning cognitive deficiency assessment and provided a detailed description of the severe TBI animal model.We found that 250 g falling weight from 2 cm height produced severe closed head injury in C57BL/6 male mice.Cognitive disorders in mice with severe closed head injury could be detected using passive avoidance test on day 7 after injury.Findings from this study indicate that weight-drop injury animal models are suitable for further screening of brain neuroprotectants and potentially are similar to those seen in human TBI.
基金supported by the Chinese Academy of Sciences Strategic Priority Research Program (XDB02050400)the National Natural Science Foundation of China (91432111)
文摘Modeling brain disorders has always been one of the key tasks in neurobiological studies. A wide range of organisms including worms, fruit ？ies, zebra？sh, and rodents have been used for modeling brain disorders. However,whether complicated neurological and psychiatric symptoms can be faithfully mimicked in animals is still debatable.In this review, we discuss key ？ndings using non-human primates to address the neural mechanisms underlying stress and anxiety behaviors, as well as technical advances for establishing genetically-engineered non-human primate models of autism spectrum disorders and other disorders.Considering the close evolutionary connections and similarity of brain structures between non-human primates and humans, together with the rapid progress in genome-editing technology, non-human primates will be indispensable for pathophysiological studies and exploring potential therapeutic methods for treating brain disorders.
