Feeding strategies of an organism depend on the multimodal sensory processing that most efficiently integrates available visual,chemosensory,and/or mechanoreceptive cues as part of their environmental adaptation.The b...Feeding strategies of an organism depend on the multimodal sensory processing that most efficiently integrates available visual,chemosensory,and/or mechanoreceptive cues as part of their environmental adaptation.The blind cavefish morph of Astyanax mexicanus has developed sensory-dependent behaviors to find food more efficiently than their eyed,surface-morph counterparts while in darkness.In the absence of light,adult cavefish have evolved enhanced behaviors,such as vibration attraction behavior(VAB),and changes in feeding angle.Here,we identified evolved differences in cavefish larval prey capture(LPC)behavior.In the dark,LPC is more efficient in cavefish than in surface fish.Furthermore,different cave populations express laterality in their LPC and strike towards prey preferentially located on their left or right sides.This suggests the occurrence,to some extent,of divergent LPC evolution among cave populations.While LPC can be triggered exclusively by a vibration stimulus in both surface and cavefish,we provide evidence that LPC is,at least partially,a multimodal sensory process different from adult VAB.We also found that a lack of food may exacerbate the laterality of LPC.Thus,we proposed a mathematical model for explaining laterality based on a balance between:(1)enlarged range of foraging field(behavioral or perceptive)due to asymmetry,(2)food abundance,and(3)disadvantages caused by laterality(unequal lateral hydrodynamic resistance when swimming,allocation of resources for the brain and receptors,and predator escape).展开更多
为了探究Sdf1-Cxcr4信号在斑马鱼(Danio rerio)后侧线系统(Posterior lateral line system,PLLs)发育中的作用,本研究首先通过原位杂交发现斑马鱼cxcr4b在迁移的侧线原基-Ⅰ和原基-Ⅱ中表达,sdf1a在原基迁移路径上表达。进一步构建了由...为了探究Sdf1-Cxcr4信号在斑马鱼(Danio rerio)后侧线系统(Posterior lateral line system,PLLs)发育中的作用,本研究首先通过原位杂交发现斑马鱼cxcr4b在迁移的侧线原基-Ⅰ和原基-Ⅱ中表达,sdf1a在原基迁移路径上表达。进一步构建了由热激启动子控制的cxcr7b过表达转基因斑马鱼,在不同时间点过表达cxcr7b抑制Sdf1-Cxcr4信号。结果表明:相较于野生型斑马鱼,过表达cxcr7b的斑马鱼的原基-Ⅰ和原基-Ⅱ的迁移明显减慢。但体侧中线神经丘的腹部迁移、间生和针脚神经丘的形成不受过表达cxcr7b的影响。本研究为理解鱼类侧线系统发育调控和多样性产生奠定基础。展开更多
The transient receptor potential vanilloid 4(TRPV4),another Ca^2+entry channel,belongs to the vanilloid subfamily and responds to a number of different physical and chemical stimuli and exists widely in mammals.Howeve...The transient receptor potential vanilloid 4(TRPV4),another Ca^2+entry channel,belongs to the vanilloid subfamily and responds to a number of different physical and chemical stimuli and exists widely in mammals.However,our understanding of the TRPV4 in fish remains poor.Therefore,we studied the TRPV4 gene from Cynoglossus semilaevis,named CsTRPV4 that encodes a putative protein of 870 amino acids common in structure and characteristic of mammalian TRPV4,including the domains of ANK repeats,six TM,TRP domain,and CaMBD.The CsTRPV4 was expressed ubiquitously in examined tissues:higher expression in the heart,spleen,testis,and eye,but lower expression in kidney and liver.Surprisingly,the expression of CsTRPV4 in lateral line was significantly higher than in many other tissues as the CsTRPV4 was expressed significantly in the free neuromasts.In addition,CsTRPV4 in the free neuromast from the larval fish was significantly expressed in the hair cells of the free neuromasts.Therefore,the free neuromasts can act as a mechano-sensor to the mechanical stimulation in molecular level in C.semilaevis,which lays a foundation for further study of the functions of the free neuromasts.展开更多
Light and scanning electron microscopy(SEM)were used to study the epidermal lateral line system of the Si-berian sturgeon(Acipenser baerii Brandt,1869).This system consists of mechanoreceptive neuromasts,ampul-lae and...Light and scanning electron microscopy(SEM)were used to study the epidermal lateral line system of the Si-berian sturgeon(Acipenser baerii Brandt,1869).This system consists of mechanoreceptive neuromasts,ampul-lae and the electroreceptive organ.The neuromasts are located in 5 pairs of cephalic and 1 pair of trunk canals and superficially in the middle and posterior pit lines that lie dorsomedially along the top of the skull immedi-ately adjacent to the otic ampullae field.Both canal neuromasts and pit organ superficial neuromasts have oppo-site polarized hair cells that are parallel along the axis of the canal and pit line,respectively.However,they dif-fer in both size and shape and in the density and length of the hair bundles.The ampullae are confined on the head,adjacent to the neuromast lines.The morphological structure of the ampullae in the Siberian sturgeon is similar to the ampullae in elasmobranchs and other primitive fish.Nevertheless,it has a relatively large mucus-filled ampulla,and a shorter and narrower canal leading to a small opening to the outer epidermal surface.We also present new information concerning the peripheral innervation of lateral line receptors in sturgeons.The re-ceptors of the lateral line system are innervated by 2 pairs of cranial nerves:anterior and posterior lateral line nerves.The peripheral processes of the anterior lateral line nerve form superficial ophthalmic,buccal,otic and anteroventral rami.The peripheral processes of the posterior lateral line nerve form middle,supratemporal and lateral rami.展开更多
基金funded by German Sumbre at the Institut de Biologie de l’ENS (IBENS), CNRS, FranceSylvie Rétaux at the Paris-Saclay Institute of Neuroscience, CNRSUniversity Paris-Saclay, France
文摘Feeding strategies of an organism depend on the multimodal sensory processing that most efficiently integrates available visual,chemosensory,and/or mechanoreceptive cues as part of their environmental adaptation.The blind cavefish morph of Astyanax mexicanus has developed sensory-dependent behaviors to find food more efficiently than their eyed,surface-morph counterparts while in darkness.In the absence of light,adult cavefish have evolved enhanced behaviors,such as vibration attraction behavior(VAB),and changes in feeding angle.Here,we identified evolved differences in cavefish larval prey capture(LPC)behavior.In the dark,LPC is more efficient in cavefish than in surface fish.Furthermore,different cave populations express laterality in their LPC and strike towards prey preferentially located on their left or right sides.This suggests the occurrence,to some extent,of divergent LPC evolution among cave populations.While LPC can be triggered exclusively by a vibration stimulus in both surface and cavefish,we provide evidence that LPC is,at least partially,a multimodal sensory process different from adult VAB.We also found that a lack of food may exacerbate the laterality of LPC.Thus,we proposed a mathematical model for explaining laterality based on a balance between:(1)enlarged range of foraging field(behavioral or perceptive)due to asymmetry,(2)food abundance,and(3)disadvantages caused by laterality(unequal lateral hydrodynamic resistance when swimming,allocation of resources for the brain and receptors,and predator escape).
文摘为了探究Sdf1-Cxcr4信号在斑马鱼(Danio rerio)后侧线系统(Posterior lateral line system,PLLs)发育中的作用,本研究首先通过原位杂交发现斑马鱼cxcr4b在迁移的侧线原基-Ⅰ和原基-Ⅱ中表达,sdf1a在原基迁移路径上表达。进一步构建了由热激启动子控制的cxcr7b过表达转基因斑马鱼,在不同时间点过表达cxcr7b抑制Sdf1-Cxcr4信号。结果表明:相较于野生型斑马鱼,过表达cxcr7b的斑马鱼的原基-Ⅰ和原基-Ⅱ的迁移明显减慢。但体侧中线神经丘的腹部迁移、间生和针脚神经丘的形成不受过表达cxcr7b的影响。本研究为理解鱼类侧线系统发育调控和多样性产生奠定基础。
基金Supported by the Earmarked Fund for Modern Agro-Industry Technology Research System(No.CARS-47-G01)the AoShan Talents Cultivation Program supported by Qingdao National Laboratory for Marine Science and Technology(No.2017ASTCP-OS04)the Qingdao Natural Science Foundation(No.12-1-4-12-(1)-jch)
文摘The transient receptor potential vanilloid 4(TRPV4),another Ca^2+entry channel,belongs to the vanilloid subfamily and responds to a number of different physical and chemical stimuli and exists widely in mammals.However,our understanding of the TRPV4 in fish remains poor.Therefore,we studied the TRPV4 gene from Cynoglossus semilaevis,named CsTRPV4 that encodes a putative protein of 870 amino acids common in structure and characteristic of mammalian TRPV4,including the domains of ANK repeats,six TM,TRP domain,and CaMBD.The CsTRPV4 was expressed ubiquitously in examined tissues:higher expression in the heart,spleen,testis,and eye,but lower expression in kidney and liver.Surprisingly,the expression of CsTRPV4 in lateral line was significantly higher than in many other tissues as the CsTRPV4 was expressed significantly in the free neuromasts.In addition,CsTRPV4 in the free neuromast from the larval fish was significantly expressed in the hair cells of the free neuromasts.Therefore,the free neuromasts can act as a mechano-sensor to the mechanical stimulation in molecular level in C.semilaevis,which lays a foundation for further study of the functions of the free neuromasts.
基金supported in part by grants from the Science and Technol ogy Commission of Shanghai Municipality 073205109the National Natural Science Foundation of China 30970365,and Hydrobiology funding project S30701.
文摘Light and scanning electron microscopy(SEM)were used to study the epidermal lateral line system of the Si-berian sturgeon(Acipenser baerii Brandt,1869).This system consists of mechanoreceptive neuromasts,ampul-lae and the electroreceptive organ.The neuromasts are located in 5 pairs of cephalic and 1 pair of trunk canals and superficially in the middle and posterior pit lines that lie dorsomedially along the top of the skull immedi-ately adjacent to the otic ampullae field.Both canal neuromasts and pit organ superficial neuromasts have oppo-site polarized hair cells that are parallel along the axis of the canal and pit line,respectively.However,they dif-fer in both size and shape and in the density and length of the hair bundles.The ampullae are confined on the head,adjacent to the neuromast lines.The morphological structure of the ampullae in the Siberian sturgeon is similar to the ampullae in elasmobranchs and other primitive fish.Nevertheless,it has a relatively large mucus-filled ampulla,and a shorter and narrower canal leading to a small opening to the outer epidermal surface.We also present new information concerning the peripheral innervation of lateral line receptors in sturgeons.The re-ceptors of the lateral line system are innervated by 2 pairs of cranial nerves:anterior and posterior lateral line nerves.The peripheral processes of the anterior lateral line nerve form superficial ophthalmic,buccal,otic and anteroventral rami.The peripheral processes of the posterior lateral line nerve form middle,supratemporal and lateral rami.
