Kinetics and isotherms of Neutral Red adsorption on peanut husk

Adsorption of Neutral Red (NR) onto peanut husk in aqueous solutions was investigated at 295 K. Experiments were carried out as function of pH, adsorbent dosage, contact time, and initial concentration. The equilibrium adsorption data were analyzed by Langmuir, Freundlich, Temkin, Dubinin-Radushkevich, and Toth isotherm models. The results indicated that the Toth and Langmuir models provided the best correlation of the experimental data. The adsorption capacity of peanut husk for the removal of NR was deter...

Neutral Red Retention by Earthworm Coelomocytes: A Biomarker of Cadmium Contamination in Soil

The earthworm Metaphire posthuma were used as a model to assess the toxic potential of cadmium incorporated into the soil by environmental or human activities. The retention period of neutral red in the lysosomes of the coelomocytes was used as a biomarker. The viability of harvested coelomocytes by a non_invasive extrusion protocol was 93% with no alteration by the dye during experimentation. The control cells retained dye for 119 and 121 min in normal soil and KCl, respectively, whereas a linear decline in the retention time in the treated earthworm coelomocytes was observed. This illustrated that the presence of cadmium caused damage to the lysosomes of the coelomocytes.

Fluorescence Microscopic Image Analysis of Nucleic Acids Based on The Capillary Flow Directed Assembly Ring of Neutral Red-nucleic Acid Supramolecular Complexes

It is critical to establish a direct and precise method with a high sensitivity and selectivity in analytical chemistry. In this research, making use of a well known phenomenon of capillary flow, we have proposed an image analysis method of nucleic acids at the price of a small amount of sample. When a droplet of the supramolecular complex solution, formed by neutral red and nucleic acids(NA) under an approximate neutral condition, was placed on the hydrophobic surface of dimethyl dichlorosilane pretreated glass slides, and it was evaporated, the supramolecular complex exhibited the periphery of the droplet due to the capillary effect, and accumulated there to form a red capillary flow directed assembly ring(CFDAR). A typical CFDAR has an outer diameter of (2 r ) about 1.18 mm and a ring width(2 δ ) of about 41 μm. Depending on the experimental conditions, a variety of CFDAR can be assembled. The experimental results are in agreement with our former theoretical discussion. It was found that when a droplet volume is 0.1 μL, the fluorescence intensity of the CFDAR formed by the NR NA is in proportion to the content of calf thymus DNA in the range of 0-0.28 ng, fish sperm DNA of 0-0.24 ng and yeast RNA of 0-0.16 ng with the limit of detection(3 σ ) of 1 7, 1.4 and 0.9 pg, respectively for the three nucleic acids.

Electrochemical Study on the Interaction Between Neutral Red and DNA

A voltammetric study of the interaction of neutral Red(NR) with DNA at a gold electrode in a phosphate buffer solution is described. After adding DNA in an NR solution, the reduction peak current of NR decreases. The binding mechahisms of NR to DNA in different pH ranges are different. The reduction peak potential of NR in a pH 7.0 phosphate buffer solution in the presence of DNA shifts positively, indicating that the binding of NR to DNA is intercalation action, but at pH=6.0 the reduction peak potential of NR shifts negatively, indicating that the binding of NR to DNA is electrostatic action. The formed complexes are DNA-NR when [NR]/[DNA]<0.18 and DNA-3NR when [NR]/[DNA]>0.35, respectively.

Investigation of the interaction of DNA and neutral red by fluorescence spectroscopic analysis

The binding characteristics of neutral red （NR） with DNA were investigated by fluorescence spectrometry. Chemometrics approach as singular value decomposition （SVD） was used to evaluate the number of spectral species in the drug-DNA binding process, and then the intrinsic binding constant of 1.6 × 10^4 in base pairs and the binding site number of 0.97 were obtained from the Scatchard plot.

Nanobiocomposite Electrochemical Biosensor Utilizing Synergic Action of Neutral Red Functionalized Carbon Nanotubes

An amperometric hydrogen peroxide biosensor using a nanobiocomposite based on neutral red modified carbon nanotubes and co-immobilized glucose oxidase and horseradish peroxidase is reported. Modification of the nanobiocomposite electrode with neutral red resulted in a sensitive, low-cost and reliable H_2O_2 sensor. The use of carbon nanotubes, as the conductive part of the composite, facilitated fast electron transfer rates. The biosensor was characterized for the influence of p H, potential and temperature. A remarkable feature of the biosensor is the detection of H_2O_2 at low applied potentials where the noise level and interferences are minimal. The sensor has a fast steady-state measuring time of 10 s with a quick response(2 s). The biosensor showed a linear range from 15 n M to 45 m M of H_2O_2 and a detection limit of 5 n M. Nafion, which is used as a binder, makes the determination free from other electroactive substances. The repeatability, reproducibility,stability and analytical performance of the sensor are very good.

Specific Lethal Effects of Trace Neutral Red on Amoeba discoides and Trichodina

[ Objective] The aim of this study was to provide the reference for the artificial breeding of aquatic animals. [ Method] Amoeba discoides and Trichodina were treated by neutral red solution with different concentrations to observe the physiological activities of organelles in their death processes. Effects of neutral red solution on the growth of common aquatic animals such as Paramecium caudatum, Euglena viridis and Brachionus plicatilis were analyzed, and the specific lethal mechanism of trace neutral red to A. discoides and Trichodina was also preliminarily studied. [ Re- suit] The neutral red solution at a concentration of 0.5 mg/L damaged the physiological function of contractile vacuole in Trichodina and also had the specific lethal effect on Tdchodina, but it had no effect on the growth and reproduction of non-parasitic protozoa and B. p/icatilis. Neutral red so- lution with certain concentration led to disorder of the physiological functions of A. discoides, such as assimilation and rejection, which was a main factor that caused the death of A. discoides. [ Conclusion] With the advantages such as targeting, safety and easiness to be oxidized and decom- posed, neutral red is an ideal drug for treating the diseases caused by A. discoides and Trichodina, and its suitable concentration is 0. 5 mg/L.

Spectroelectrochemical Investigation on Neutral Red

Neutral Red can be used as an indicator, a stain reagent or a mediator compound in the studies of biological redox systems. No reports dealing with the electrode process of Neutral Red, especially, about its kinetics have been published. In this paper we report the determinations of formal reduction potentials, the number of electrons transferred, diffusion coefficient as well as the rate constant of heterogeneous electron transfer

The Radiation Degradation of Neutral Red Solution by <i>γ</i>-Ray

Neutral red is kind of biologic colourant and acidity-basicity indicator. Radiation degradation of neutral red in aqueous solution was done by γ-Ray. The removal rate of chemical oxygen demand, total organic carbon, chroma and the changing of pH value were studied under various conditions. With the increase of absorbed doses, the chemical oxygen demand and chroma decreased conspicuously. The absorbed dose rate has little effect on the degradation of neutral red. When the absorbed doses are the same, the chemical oxygen demand and chroma decreased more obviously with the increase of neutral red concentration. Weak basic condition and proper H2O2 addiation are propitious to removal of chemical oxygen demand of neutral red.

Determination of Epinephrine Using Poly(Neutral Red)Modified Carbon Fibre Microelectrodes by Anodic Stripping Voltammetry

Poly(neutral red) film modified carbon fibre microelectrodes offer substantial improvement in voltammetric sensitivity and selectivity towards epinephrine (EP). The poly(neutral red) film was electropolymerized by cycling the potential between -0.8 V and +0.8 V. The anodic stripping voltammetric response for EP was found to be dependent on accumulation time and potential. By using a poly(neutral red) film modified carbon fibre microelectrode with a 1 min preconcentration at -1.2 V in biological phosphate buffer solution (pH=7.4), a good linear relationship between the anodic stripping peak currents and EP concentrations was obtained in the range of 2.0×10 -7 ￣ 2.0×10 -5 mol/L. The detection limit was up to 9.0 ×10 -8 mol/L. Moreover, 400 times higher concentrations of vitamin C did not interfere with the measurement of EP. This method was used for determining EP concentrations in epinephrine hydrochloride injection solution with satisfactory results.

The synthesis of mono-6-thio-β-cyclodextrin capped CdTe QDs and its interaction with neutral red

This paper presents a novel method for the synthesis of CdTe quantum dots (QDs) capped with β-cyclodextrin in aqueous solution using both TGA and mono-6-thio-β-CD as stabilizers.The interaction between mono-6-thio-β-CD-CdTe QDs and neutral red (NR) was studied with fluorescence,UV-absorption and the resonance Rayleigh scattering spectrum.When its concentration was over 7.5 × 10-6 mol/L,the neutral red began to aggregate on the surface of the mono-6-thio-β-CD-CdTe QDs,which resulted in the mono-6-thio-β-CD-CdTe QDs particle size increasing,the sharply quenched fluorescence,and the marked increase of RRS intensity.

碳中和背景下的赤泥综合利用现状

作为氧化铝工业过程伴生的固体废弃物,赤泥的规模化消纳与高值化利用一直以来是能源、化工领域的研究热点。在“碳中和”目标的引领下,有必要对赤泥的无害化处置及综合利用技术进行重新归纳与审视。首先从赤泥的基本理化性质出发,面向不同CO_(2)浓度的应用场合,分析了赤泥直接在CO_(2)捕集中的利用现状,对捕集装置、技术及赤泥组分的转化进行了综述。其次,基于赤泥自身丰富的多孔结构与碱金属类的化学组分,论述了以赤泥为原料的高值固体多孔材料(如沸石分子筛)合成与应用的相关研究,涉及材料制备工艺、CO_(2)捕集效果等。最后,从赤泥基固体催化剂的角度,论述了其在CO_(2)加氢、化学链燃烧等领域的催化效果。为赤泥在CO_(2)捕集转化领域的应用现状提供了全面的认识。

KELEA: A Natural Energy That Seemingly Reduces Intermolecular Hydrogen Bonding in Water and Other Liquids

The alternative cellular energy (ACE) pathway was initially identified as a non-immunological defense mechanism against virus infections. It is particularly relevant to the suppression of stealth adapted viruses, which are not normally recognized by the cellular immune system. Many of the methods able to enhance the ACE pathway are consistent with the transfer of a natural energy to the body’s fluids. Additional support for this premise is provided in this paper. The vapor pressure and rate of evaporation of activated water, ethanol and gasoline increase to beyond atmospheric pressure over time. The term KELEA (kinetic energy limiting electrostatic attraction) is proposed for a natural energy that increases the volatility of fluids, seemingly through the loosening of intermolecular hydrogen bonding. KELEA activated fluids have many potential health, agricultural and industrial applications, as well as providing the opportunity for fundamental research.

4种百合不同花期花香成分鉴定与关键基因表达研究

使用顶空固相微萃取(HS-SPME)和气相色谱-质谱(GC-MS)联用技术,对4个百合(Lilium spp.)品种初花期和盛花期的花朵进行花香成分分析,并利用实时荧光定量PCR(qRT-PCR)和中性红染色试验,分别对不同品种百合初花期和盛花期花朵进行6个花香合成关键基因相对表达量和花香释放部位的测定.结果表明,4个百合品种初花期和盛花期共测定出61种挥发性化合物,盛花期花朵挥发物总含量均高于初花期,含量较高的成分有芳樟醇、(Z)-β-罗勒烯、桉树脑、(+)-柠檬烯、月桂烯、苯甲酸甲酯等.差异挥发物的筛选结果显示,4个品种共筛选出差异挥发物44种.将61种挥发性化合物分为9大类,4个品种初花期与盛花期花香的主要成分为萜烯类和酯类化合物. qRT-PCR结果表明,PAL、PAL3、DXS、TPS这4个基因在4个品种盛花期的相对表达量均高于初花期,而BSMT在‘西伯利亚’盛花期,DXR基因在‘竞争’和‘木门’盛花期的相对表达量低于初花期.中性红染色试验结果显示,盛花期花朵染色程度高于初花期,且均在花瓣的上半部反卷处染色最深,说明该部位为主要的花香物质释放区域.

中性脂含量高的微拟球藻藻株的快速筛选

微拟球藻(Nannochloropsis oceanica)生长速度快、油脂含量高,已成为生产生物柴油的理想材料。为获得中性脂产率高的藻株,本研究从染色时间和染色浓度两个方面优化了尼罗红染色测定中性脂含量的方法,对不同育种方法得到的75株微拟球藻藻株的产油能力进行了评价。采用优化后的染色条件(尼罗红浓度1 mg/mL,染色时间10 min),测定了第9天微藻的中性脂相对含量及对应的生物量,获得藻株中性脂的相对产率。研究表明,与原始藻株相比,088号转基因藻株相对产率为252.16%,甲基磺酸乙酯(EMS)诱变藻株101号相对产率为259.02%,亚硝基胍(NTG)诱变藻株231号相对产率为323.84%。本研究获得了3株中性脂产率高的微拟球藻藻株,这3株藻株有望应用于生物柴油的生产,研究结果可为高油脂含量微藻的快速筛选提供参考。

基于中性红-碳纳米复合材料的化学修饰电极检测亚硝酸盐

为了实现亚硝酸盐的灵敏检测,利用溶液剥离法和水合肼还原法制备了MWCNT@rGONR复合材料,并通过SEM和XRD等测试手段进行材料表征。将制备的复合材料滴涂在玻碳电极表面,借助简单可控的电聚合方法将中性红修饰到电极上,最终制备了PNR/MWCNT@rGONR/GCE。采用计时电流法对NO_(2)^(-)进行定量检测,结果表明,氧化峰电流与亚硝酸盐浓度在1.0×10^(-7)~1.9×10^(-3)mol/L范围内呈良好的线性关系,检出限为7.1×10^(-8)mol/L(S/N=3);同时,所制备的复合电极表现出良好的选择性和重现性。用此复合电极对某石化循环冷水进行实际水样的测定,回收率为93.26%~97.42%,相对标准偏差低于5.32%,结果较为理想。

蛇纹石中和渣制备氧化铁红和回收镍的工艺研究

采用硫酸浸出蛇纹石中和渣,并用红透山硫铁矿还原酸浸液;然后用NaOH沉淀去除Al和Cr,用Na_(2)S沉淀回收Ni、Co、Mn;再经碳酸氢铵沉淀铁,焙烧制备氧化铁红。采用X射线衍射仪(XRD)、扫描电镜(SEM)和X射线荧光光谱分析(XRF)等对硫铁矿还原过程进行研究。研究结果表明:在反应温度85℃、反应时间260 min条件下,酸浸液中Fe^(3+)完全被硫铁矿还原,pH从0.94提高到2.90;还原后硫铁矿由片状团聚体转变为球形团聚体,硫产物由27.81%S、33.96%H_(2)SO_(4)和38.22%H_(2)S(质量分数)组成,硫铁矿表面没有单质硫覆盖。用NaOH中和沉淀可完全去除Al和Cr。Na_(2)S沉淀回收93.01%镍、91.62%钴,得到镍含量为13.47%(质量分数)的Ni、Co和Mn沉淀渣,达到镍精矿YS/T 340—2014中1级品标准。将纯化后的硫酸亚铁溶液沉淀,在600℃焙烧得到氧化铁红,颜色与三环颜料公司的H101产品相似,着色力达125.45%,质量符合GB/T1863—2008的要求。