You may still remember the story about the race between the big white rabbit and little tortoise, in which the over-proud rabbit lost. However, the White Rabbit toffees produced by the Shanghai Guansheng Yuan (Group) ...You may still remember the story about the race between the big white rabbit and little tortoise, in which the over-proud rabbit lost. However, the White Rabbit toffees produced by the Shanghai Guansheng Yuan (Group) Co., Ltd. are famous-brand products in China. This White Rabbit not only is the cham-展开更多
The open reading frame (ORF) of hAPOA1 was inserted into the prokaryotic expression vector pGEX-4T-1 to construct the recombinant plasmid pGEX- 4T-I-hAPOA1, which was then transformed into Escherichia coil strain BL...The open reading frame (ORF) of hAPOA1 was inserted into the prokaryotic expression vector pGEX-4T-1 to construct the recombinant plasmid pGEX- 4T-I-hAPOA1, which was then transformed into Escherichia coil strain BL21. The expression of target fusion protein was induced with isopropyl β-D-l-thiogalacto- pyranoside (IPTG). The purified fusion protein in inclusion bodies was used to immunize New Zealand white rabbits to prepare hAPOA1 antiserum and the antibody titer was detected with indirect enzyme-linked immunosorbent assay (ID-EL1SA). ID-ELISA and Western Blot proved that rabbit polyclonal antibody with a high titer of 1 : 40 000 was produced, which may bring considerable economic benefits.展开更多
文摘You may still remember the story about the race between the big white rabbit and little tortoise, in which the over-proud rabbit lost. However, the White Rabbit toffees produced by the Shanghai Guansheng Yuan (Group) Co., Ltd. are famous-brand products in China. This White Rabbit not only is the cham-
基金Supported by the Agricultural Science Independent Innovation Fund Jiangsu Province[CX(16)1326]
文摘The open reading frame (ORF) of hAPOA1 was inserted into the prokaryotic expression vector pGEX-4T-1 to construct the recombinant plasmid pGEX- 4T-I-hAPOA1, which was then transformed into Escherichia coil strain BL21. The expression of target fusion protein was induced with isopropyl β-D-l-thiogalacto- pyranoside (IPTG). The purified fusion protein in inclusion bodies was used to immunize New Zealand white rabbits to prepare hAPOA1 antiserum and the antibody titer was detected with indirect enzyme-linked immunosorbent assay (ID-EL1SA). ID-ELISA and Western Blot proved that rabbit polyclonal antibody with a high titer of 1 : 40 000 was produced, which may bring considerable economic benefits.