Effects of acupuncture and moxibustion on chromatin remodeling-related enzymes in the colon tissue of rats with Crohn disease

Objective:To investigate the regulatory effects of acupuncture and moxibustion on chromatin remodeling complex core catalytic subunit of Brahma-related gene 1(Brg1),histone deacetylase(HDAC)3,HDAC9,and males absent on the first(MOF)in the colon tissue of rats with Crohn disease(CD).Methods:Using the random number table method,60 male Sprague-Dawley rats were divided into 5 groups,including a normal group,a model group,an acupuncture group,a medicinal cake-insulated moxibustion group,and an acupuncture-moxibustion group,with 12 rats in each group.CD rat models were prepared using 2,4,6-trinitrobenzene sulfonic acid(TNBS)in all groups except the normal group.The normal and model groups received no interventions.In the acupuncture group,rats were intervened with acupuncture at bilateral Zusanli(ST36)and Shangjuxu(ST37),20 min/session,once a day.The medicinal cake-insulated moxibustion group received medicinal cake-insulated moxibustion at Qihai(CV6)and bilateral Tianshu(ST25)with 2 cones per point per session,once a day.The acupuncture-moxibustion group received both acupuncture and moxibustion interventions simultaneously.Each intervention was performed for 10 consecutive days.Observations included general condition,disease activity,macroscopic damage,and pathological changes in the rat’s colon tissue.Realtime fluorescence quantitative polymerase chain reaction was used to measure the mRNA expression of chromatin remodeling-related enzymes Brg1,HDAC3,and HDAC9,while Western blotting detected the protein expression of Brg1,HDAC3,HDAC9,and MOF in rat’s colon tissue.Results:The model group showed significantly increased diarrhea score,occult blood score,macroscopic damage score of colon tissue,and colon macroscopic damage index(CMDI)score,as well as elevated mRNA expression levels of HDAC3 and HDAC9,protein expression levels of HDAC3,HDAC9,and MOF,and decreased mRNA and protein expression levels of Brg1 compared to the normal group(P<0.01).In contrast,compared to the model group,the diarrhea score,occult blood score,macroscopic damage score,CMDI score,mRNA expression levels of HDAC3 and HDAC9,and protein expression levels of HDAC3,HDAC9,and MOF were significantly reduced(P<0.05 or P<0.01),while the mRNA and protein expression levels of Brg1 were significantly increased(P<0.01)in the acupuncture group,the medicinal cake-insulated moxibustion group,and the acupuncture-moxibustion group.Conclusion:Both medicinal cake-insulated moxibustion and acupuncture,either used alone or in combination,can regulate the abnormal expression of chromatin remodeling-related enzymes Brg1,HDAC3,HDAC9,and MOF in the colon tissue,thus reducing colon inflammation in CD rats.

Study of the mechanisms of acupuncture and moxibustion treatmentfor ulcerative colitis ratsinview of the gene expression of cytokines

AIM:To observe the effect of acupuncture and moxibustion on the expression of IL-1beta and IL-6 mRNA in ulcerative colitis rats.METHODS:The SD rat ulcerative colitis model was created by immunological method associated with local stimulation. Colonic mucosa was prepared from human fresh surgical colonic specimens, homogenized by adding appropriate amount of normal saline and centrifuged at 3000r/min. The supernatant was collected for measurement of protein conentration and then mixed with Freund adjuvant. This antigen fluid was first injected into the plantae of the model group rats, and then into their plantae, dorsa, inguina and abdominal cavities (noFreund adjuvant for the last injection) again on the 10th, 17th, 24th and 31st day. When a certain titer of serum anti colonic antibody was reached, 2% formalin and antigen fluid (no Freund adjuvant) were administered separately by enema. The ulcerative colitis rat model was thus set up. The animals were randomly divided into four groups: model control group (MC, n = 8), electro acupuncture group (EA, n = 8), herbs partition moxibustion group (HPM 8), normal control group (NC,n = 8). HPM: Moxa cones made of refined mugwort floss were placed on the medicinal pad (medicinal pad dispensing: Radix Aconiti praeparata, cortex Cinnamomi, etc) for Qihai (RN 6) and Tianshu (ST 25, bilateral) and ignited. Two moxa cones were used for each acupoint once a day and 14 times in all. EA: Tianshu (bilateral) and Qihai were stimulated by the intermittent pulse with 2Hz frequency, 4mA intensity for 20 minutes once a day and 14 times in all. After treatment, rats of all four groups were killed simultaneously. The spleen was separated and the distal colon was dissected. Total tissue RNA was isolated by the guanidinium thiocyanate phenol chloroform extraction method. RT-PCR technique was used to study the expression of IL-1 beta and IL-6 mRNA.RESULTS:IL-1 beta and IL-6 mRNAs were not detected in the spleen and colonic mucosa of the NC rats, whereas they were significantly expressed in that of the MC rats.IL-1 beta and IL-6 mRNAs were markedly lower in the EA and HPM rats than that in MC rats. There was no significant difference between the levels of IL-1 beta and IL-6 mRNAs in the EA and HPM rats. The expressions of IL-1 beta and IL-6 mRNAs were nearly the same in the spleen and colon of all groups.CONCLUSION:Acupuncture and moxibustion greatly inhibited the expression of IL-1 beta and IL-6 mRNA in the experimental ulcerative colitis rats.

OBSERVATION OF THE EFFICACY OF ACUPUNCTURE AND MOXIBUSTION IN 62 CASES OF CHRONIC COLITIS

62 patients with chronic colitis were randomly divided into two groups. Acupuncture and moxibustion at acupoints such as Tianshu (St 25), Guanyuan (Ren 4) were applied in the treatment group, and western drugs were applied in the control group. The results showed that acupuncture and moxibustion had a marked curative effective with few side effects, and therefore was readily acceptable to the patients.

Twenty One Cases of Vertebral-Artery-Type Cervical Spondylosis Treated with Acupuncture and Moxibustion

The vertebral-artery-type cervical spondylosis iscommonly encountered in the middle aged and oldpeople.The author has treated 21 cases of vertebral-artery-type cervical spondylosis by acupuncture andmoxibustion,with the other 19 cases treated only byacupuncture as the controls.The results are reportedas follows.Clinical

A CLINICAL STUDY ON ACUPUNCTURE FOR PREVENTION AND TREATMENT OF TOXIC SIDEEFFECTS DURING RADIOTHERAPY AND CHEMOTHERAPY

Radiotherapy and chemotherapy arecommonly used in the treatment of tumors.However,such toxic side-effects as leukopenia,gastrointestinal or systemic reactions occurredin the treatment are so serious that somepatients can not tolerate it and even abandonthe treatment.Recently,some doctors

The Present State and Perspective in Treatment of Primary Osteoporosis by Acupuncture and Moxibustion

Primary osteoporosis, a commonly encountered metabolic bone disease in the postmenopausal women and the aged people, can be classified by modern medicine into postmenopausal osteoporosis (Type I) and senile osteoporosis (Type II). The disease seriously affects health and quality of life of the people as it often cause ostealgia, fracture and the secondary symptoms or diseases. Presently, the pharmacotherapy (including both Chinese herbal drugs and western drugs) remains the first among all other therapeutic methods which are mainly adopted in treatment of the disease at home and abroad. Studies related have been curried out quite early and systematically, and considerable progress has been made, but limit of the pharmacotherapy has also been found. Certain non-drug treatments (such as dietetic therapy, physical exercise, acupuncture and moxibustion, and qigong, especially acupuncture and moxibustion therapy, although with a late start, have been proved effective with satisfactory results. The following is a summary of all the contributions concerned.……

DEVELOPMENT ON CLINICAL TREATMENT OF CONSTIPATION WITH ACUPUNCTURE AND MOXIBUSTION

In the present paper, the authors review recent developmarrt of clinical treatment of constipation with acupunctire and moxibustion from ① body acupuncture therapy including special needling manipulations, as Ziwu Daojiu Needling, etc. and acupoint combination,②electrcacupunture therapy, ③ scalp-acupuncture therapy, ④ auricular acupuncture therapy, ⑤ needleembedding and acupoint-catgut-embedding therapies, ⑥ acupoint-application therapy, ⑦ combinged acupuncture aud medication therapy, ⑧ other therapies such as cupping therapy and holo-therapy, and ⑨ considerations about the action of acupuncture in the treatmnt of constipation and its prospect. Up to now, researches on the effect of acupuncture in treating constipation are seldom seen, thus, much attention should be paid about this by clinical and experimental researchers.

CHINESE HUAI TANG NINE ACUPUNCTURE NEEDLES: RESEARCH ON THE NEW NINE ACUPUNCTURE NEEDLES THERAPY AND THE NEEDLING INSTRUMENTS (CONTINUED)

PART TWOThe Research on Chinese Huai TangNine Acupuncture Needles TherapyI The theoretical basis of ChineseHuai Tang Nine Needles Therapy.1) The kernel of the theoretical basis ofChinese Huai Tang Nine Needles Therapy.Chinese Huai Tang Nine Needles Therapyis to give full play to the treatment action of thespecifity and entirety of each needle. The speci-fity means that each needle has its own

A SURVEY OF EPILEPSY TREATED WITH ACUPUNCTURE AND MOXIBUSTION IN RECENT YEARS

In this paper,we have introduced the developing tendencies and features of the treat-ment of epilepsy with acupuncture and moxibustion.studies on the naechanism of the therapeutic ef-fect.the evaluation of the clinical effect and some problems in the last 10 years.Moreover,we haveput forward our opinions for improving the treatment in future.

APPLICATION OF ACUPUNCTURE AND MOXIBUSTION FOR KEEPING SHAPE

On the basis of TCM diferentiation, the authors have treated 359 adult female cases of non-obesity with undesirable body shape by combined application of body acupuncture and moxibustion and auricular acupuncture, and achieved quite good remoulding and orthopedic results, suggesting that acupuncture and moxibustion can very effectively regulate the somatotypic indexes of body weight, circumference of the chest, loin, hip and thigh, the ratio of the loin to hip, sebum thickness, obesity degree, body mass index and body fat percentage of the cases in the overweight group and orthopedic groups 1,2. It is therefore concluded that acupuncture and moxibustion is a good therapy for obtaining a graceful body shape.

23 CASES OF CHRONIC NONSPECIFIC ULCERATIVE COLITIS TREATED BY ACUPUNCTURE AND MOXIBUSTION

In recent years, the author has treated 23 in-patients and out-patients with chronic nonspecific ulcerative colitis and achieved good therapeutic results as reported in the following. Clinical Data In this series, there were 23 cases, 9 males and 14 females, ranging in age from 29 to 68 years, averaging 33.8 years. The duration of disease was 2 to 23 years, with a mean of 11.5 years. All the patients had symptoms and signs of the disease in varying degrees

The effect of acupuncture and selection of acupoints on myelosuppression after chemotherapy

Objective:To evaluate the effect of acupuncture on myelosuppression after chemotherapy and summarize its acupoint selection strategy.Methods:After a systematic search,an acupoint database on myelosuppression after chemotherapy was constructed.A meta-analysis was conducted to assess the efficacy of acupuncture.On this basis,association analysis and cluster analysis were performed to explore the distribution of acupoints in SPSS Modeler18.0 and SPSS 22.0.Results:40 studies with 2988 patients were included.The white blood cell count,platelet count,red blood cell count,and hemoglobin were significantly higher in patients receiving acupuncture versus convention therapy exclusively.The two groups,however,did not differ in neutrophils count.Most acupoints,mainly distributed in the limbs and the back,were from the bladder meridian,the stomach meridian,and the spleen meridian.The lower sea and transport points were the most preferred specific points,in which ST36 and SP6 ranked highest in use.ST36-RN6,SP6-SP10,and ST36-SP10 turned out to be the most significant correlations in association rule mining.In cluster analysis,LI4-RN12,ST36-RN4-RN6 were grouped for their high similarity.Conclusion:For patients with myelosuppression after chemotherapy,acupuncture significantly inhibited the decline in blood cell components(white blood cell count,platelet count,red blood cell count,and hemoglobin)except for neutrophils.As the central combination of acupuncture prescriptions for this population,ST36-SP6-RN6-SP10-RN4 presented promising prospects and deserved more exploration for clinical use.

STUDY ON ACUPUNCTURE AND MOXIBUSTION THERAPY FOR FEMALE URETHRAL SYNDROME

Among 180 patients with female urethral syndrome, 128 were treated by acupuncture and moxibustion and 52 by western medicine as controls. The short-term effective rate in the acupuncture and moxibustion group was 90.6% and the long-term effective rate, 80.4%; whereas the short-term effective rate of the control group was 26.9% (P 0.05). Sixty-nine cases from the acupuncture and moxibustion group and 39 from the control group were subjected before and after treatment to determinations of the maximal bladder pressure, maximal abdominal pressure, bladder-neck pressure, and maximal urethral closure pressure during urination. All these indexes were decreased remarkably in the acupuncture and moxibustion group, while no changes were observed in the control group.

A Case of Intractable Hiccup Treated by Acupuncture and Moxibustion

Hiccup is a syndrome characterized by continuous sound in the throat,short and frequent sound,and difficult to stop[1].Although the disease is not life-threatening,it seriously affects the quality of life of patients.Long term intractable hiccup will also have an impact on the psychology of patients and bring great trouble to patients.Hiccup is equivalent to diaphragmatic spasm in western medicine and involves a variety of systemic diseases[2].At present,the treatment of this disease includes drugs and nerve block,but the curative effect and prognosis are not ideal.Acupuncture and moxibustion,as a green and simple treatment method,has unique curative effect in the treatment of hiccup.Now the test of one case of intractable hiccup caused by ineffective treatment is as follows.

TREATMENT OF TENNIS ELBOW BY ACUPUNCTURE AND MOXIBUSTION

Tennis elbow, or external humeral epicondylitis, is a lingering disease with an unbearable pain. It is very difficult to be treated with a higher recurrent rate. The use of acupuncture and moxibustion in treatment of tennis elbow has been appreciated. This review will focus on the methods and the therapeutic effects of treating the disorder by acupuncture and moxibustion in recent years.

ON THE INDICATIONS OF ACUPUNCTURE AND MOXIBUSTION

Though with Wide Indications, Acupuncture and Moxibustion are not the Remedy for All Ills Treating diseases with acupuncture-moxibustion has a long history in China, and rich experience has been accumulated in its application. According to a statistics in early 1980’s by prof. Jiao Guorui, a senior researcher from China Academy of Traditional Chinese Medicine, acupuncture-moxibustion therapy has at least been used to treat 307 kinds of diseases, among

Treatment of 482 Cases of Cervical Spondylopathy by Combining Point-injection and Needle-warming via Moxibustion

From January 1993 to December 1996, we treated 482 cases of cervical spondylopathy with a combined method of point-injection and needle-warming via moxibustion. Except for the cases of sympathetic nerve type and spinal cord type, the combined method was superior to traction therapy in the control group and reported as follows.Clinical DataThe Criteria of Diagnosis and Curative Effect in TCM issued by the State Administrative Bureau of TCM and Pharmacy in 1994 was adopted for the enrollment of patients of cervical spondylopathy and the pathological typing. Only the patients who had completed the treatment and with complete records were collected for analysis.

Clinical Research on Treatment of Migraine with Combination of Characteristic and Superior Techniques of Zhuang Medicine

Objective:To study the application effect of the combination of special advantages of Zhuang medical technology for migraine patients.Methods:80 patients with migraine were selected and all were admitted to Mingxiu Branch of International Zhuang Hospital Affiliated to Guangxi University of Traditional Chinese Medicine from February 2019 to February 2020.Random number table method was used to divide patients into two groups,with 40 patients in each group.Among them,the patients in the study group were treated with the therapy combining characteristics of Zhuang medicine and superior technology,that is,Zhuang medicine meridian tendon therapy+Zhuang medicine lotus needle cupping blood stasis removal therapy+Zhuang medicine thread moxibustion therapy+Zhuang medicine acupuncture therapy;the patients in the control group were treated with conventional Western medicine.The efficacy of the two treatments were compared.Results:After treatment,the total effective rate of clinical treatment in the study group was higher than that in the control group(P<0.05),and the visual analogue scale(VAS)score and integral improvement rate were higher than those in the control group(P<0.05).The onset time of action was statistically shorter than that of the control group(P<0.05).There was a statistically significant difference in the adverse reaction rate between the two groups(P<0.05).Conclusion:The combination of Zhuang medicine’s characteristic and superior techniques for patients with migraine has a significant curative effect.

Acupuncture, Moxibustion, and Combination Therapies for Insomnia

Insomnia, a common sleep disorder, affects general well-being, hastens the onset of other diseases, and impairs work performance. Hypnotic medications are efficacious in the short term but have obvious side effects. Acupuncture, often used to treat insomnia in traditional Chinese medicine (TCM), is considered to be beneficial in restoring the normal sleep-wake cycle by regulating and restoring the natural flow of qi (energy power). The three main TCM theories for treating insomnia by acupuncture are the tranquilization disturbance, zangfu disturbance (disequilibrium of internal organs), and imbalance of yin and yang theories. Moxibustion, another treatment for insomnia, is usually combined with acupuncture. Acupuncture and moxibustion with tuina (exercise massage), acupuncture with Chinese herbal injection, electroacupuncture, and acupuncture with medication or psychotherapy are other interventions. Some acupuncture-based methods such as needle-rolling acupuncture, auricular acupoint plaster therapy, phlebotomy, and acupoint catgut-embedding therapy are used as well. Although most clinical trials have shown that acupuncture and its combination therapies are significantly effective in insomnia, the beneficial effects may have been overvalued, because of small sample size, nonstrict inclusion and exclusion criteria, flawed methodology, short follow-up, or nonstandardized evaluation. Therefore, clinical studies of high methodological quality are needed to verify the efficacy of acupuncture, moxibustion, and other combination therapies in insomnia.

TREATMENT OF 42 CASES OF ASTHMA WITH ACUPUNCTURE PLUS DRUG-CAKE MOXIBUSTION

In the present paper, 42 cases of asthma patients were treated with acupuncture anddrug-cake moxibustion at Dazhui (GV 14), Feishu (BL 13), Jueyinshu (BL 14), etc.. After treatment, of the 42 cases, 10 (23. 8 % ) had controlled their symptoms, 15 (35. 7 % ) experienced markedimprovement, l3 (31. 0 % ) had improvement and 4 (9. 5 % ) had not any effect, with the effectiverate of 90. 5 %.