To enhance the yield potential of an elite indica rice cultivar,an introgression(BC_3-derived) line of IR64,YTH288,was developed using a new-plant-type cultivar,IR66215-44-2-3,as a donor parent.YTH288 has agronomicall...To enhance the yield potential of an elite indica rice cultivar,an introgression(BC_3-derived) line of IR64,YTH288,was developed using a new-plant-type cultivar,IR66215-44-2-3,as a donor parent.YTH288 has agronomically valuable characteristics such as large panicles,few unproductive tillers,and large leaves inherited from NPT.To identify the genetic basis of these traits,we used 167 F_2 plants derived from a cross between IR64 and YTH288 to conduct QTL analysis for five agronomic traits:days to heading(DTH),culm length(CL),flag leaf length(FLL),flag leaf width(FLW),and filled spikelet number per panicle(FSN).Six putative QTL were detected:four on chromosome4(for CL,FLL,FLW,and FSN) and two on chromosome 2(for DTH and FLL).All QTL with the IR66215-44-2-3 allele,except that for FLL on chromosome 2,had positive effects on each trait.To confirm the effects of these putative QTL,we developed NILs with the IR64 genetic background by marker-assisted selection.We observed significant differences in several agronomic traits between IR64 and NILs that carried these QTL on chromosomes2 and 4.Additionally,four IR64-NILs carrying chromosomal segments derived from different NPT varieties on the long arm of chromosome 4 exhibited similar pleiotropic To enhance the yield potential of an elite indica rice cultivar, an introgression(BC3-derived) line of IR64, YTH288, was developed using a new-plant-type cultivar,IR66215-44-2-3, as a donor parent. YTH288 has agronomically valuable characteristics such as large panicles, few unproductive tillers, and large leaves inherited from NPT.To identify the genetic basis of these traits, we used 167 F2 plants derived from a cross between IR64 and YTH288 to conduct QTL analysis for five agronomic traits: days to heading(DTH), culm length(CL), flag leaf length(FLL), flag leaf width(FLW), and filled spikelet number per panicle(FSN). Six putative QTL were detected: four on chromosome4(for CL, FLL, FLW, and FSN) and two on chromosome 2(for DTH and FLL). All QTL with the IR66215-44-2-3 allele, except that for FLL on chromosome 2, had positive effects on each trait. To confirm the effects of these putative QTL, we developed NILs with the IR64 genetic background by marker-assisted selection. We observed significant differences in several agronomic traits between IR64 and NILs that carried these QTL on chromosomes2 and 4. Additionally, four IR64-NILs carrying chromosomal segments derived from different NPT varieties on the long arm of chromosome 4 exhibited similar pleiotropiceffects for unique agronomic traits. These NILs can be used as research materials for studying each trait and as breeding materials for yield improvement of indica rice cultivars.展开更多
Protein sources in the diet of people living in semi-arid land of Kenya are lacking and if available it is costly to them. In terms of safe food and a healthy food supply, cowpeas (Vigna unguiculata.) are a significan...Protein sources in the diet of people living in semi-arid land of Kenya are lacking and if available it is costly to them. In terms of safe food and a healthy food supply, cowpeas (Vigna unguiculata.) are a significant source of protein, carbohydrates, and minerals especially for poor populations in the Kenya, it is said to be poor man’s meat. The aim of this study was to determine nutritional composition of newly bred ten cowpea lines and five varieties commonly grown in Eastern Kenya of Kitui, Machakos and Makueni counties to understand their potential utilization in curbing rising food and nutrition insecurity in arid and semi-arid lands ASALs and in any other food applications in Kenya. The experiment was arranged in Completely Randomized Design (CRD) whereby proximate composition and minerals were determined using standard AOAC and AACC methods and technological characteristics checked using modified methods used by other researchers. Collected data were subjected to Analysis of Variance (ANOVA) using SAS (2006) version 9.1, mean separation was done using Tukey’s Honestly Significant Difference (HSD) method at 5% level of significance. Cowpeas composition ranged from 12.28% - 13.35% for moisture content, 49.37% - 55.74% for total carbohydrates, 2.99% - 3.34% for crude ash, 0.13% - 0.81% for crude lipids, 23.37% - 29.70% for crude protein and 1.40% - 4.34% for crude fibers. Cowpea samples recorded highest percentage of essential amino acids (60.71%) and non-essential amino acids (39.29%). Minerals ranged from 1.97 - 2.69 mg/100g for calcium, 3.23 - 3.90 mg/100g for magnesium, 205.53 - 223.30 mg/100g for sodium, 0.80 - 1.23 mg/100g for zinc, 1071.15 - 1152.62 mg/100g for potassium and 0.62 - 1.06 mg/100g for phosphorus. For technological properties, lines absorbed water equivalent to their weights and they were comparable to varieties grown in the region. From the results it showed that cowpea line IT97K-1042-3, TEXAN PINKIYE, TX123, IT85F-867-5, IT82D-889-1 and IT82D-889 have desirable attributes such as high crude protein contents, good water absorption capacities and volumetric expansion. They compared well with existing K80 variety. These cowpea lines could possibly be bred and combined into a single cowpea line and further improved by breeders to have other good properties such as higher levels of water absorption during soaking hence reduced cooking times. Therefore, this work has shown that cowpea lines developed can be used as food security crop, industrial food applications and enriching foods of low protein like in complementary foods for healthy food supply in Eastern Kenya.展开更多
An analytic hierarchy process(AHP)was employed to assess the applicability of 18 new and superior varieties of flowers in Hefei City flower border applications.A total of 12 indicators were selected from three distinc...An analytic hierarchy process(AHP)was employed to assess the applicability of 18 new and superior varieties of flowers in Hefei City flower border applications.A total of 12 indicators were selected from three distinct aspects of adaptability,ornamental characteristics and use traits,in order to establish a comprehensive evaluation model.The results demonstrate that grade I(J≥2.685)exhibits excellent application value,encompassing six species of plants,such asHydrangeamacrophylla‘Endless Summer’;grade II(2.684≤J≤2.420)is also of notable application value,encompassing five species of plants,such asCallistemonrigidus;grade III(2.419≤J≤2.615)is of average application value,including five species of plants,such asCrocosmiacrocosmiflora;grade IV(J≤2.16)is of relatively poor application value.The evaluation results may be utilized as a theoretical reference for the promotion of new and superior varieties in the flower border of Hefei.展开更多
Ⅱ-you 92: a new hybrid rice for late seasonⅡ-you 92 is an indica hybrid rice that was developed by Jinhua Agri Res Inst, Zhejiang Province.Ⅱ-you 92 has a suitable growth duration of 122-125 d as double-cropping lat...Ⅱ-you 92: a new hybrid rice for late seasonⅡ-you 92 is an indica hybrid rice that was developed by Jinhua Agri Res Inst, Zhejiang Province.Ⅱ-you 92 has a suitable growth duration of 122-125 d as double-cropping late season rice, 3-4 dshorter than that of Shanyou 64 and 6-7 d shorter than Shanyou 10. It has a compact plant type, high tiller-ing ability and plant height of 85-90 cn. A number of 3.4-3.6 million effective panicles per ha can be展开更多
Qixiuzhan 3:a new high-yielding, good-quality indica rice varietyBy crossing Qishanzhan with Xinxiu 299, a new variety Qixiuzhan 3 was developed by the Rice Res Inst ofGuangdong Acad of Agri Sci.Being planted in the e...Qixiuzhan 3:a new high-yielding, good-quality indica rice varietyBy crossing Qishanzhan with Xinxiu 299, a new variety Qixiuzhan 3 was developed by the Rice Res Inst ofGuangdong Acad of Agri Sci.Being planted in the early season, this variety belongs to mid-late-maturity rice, with the same ma-展开更多
The authors discribed a new polystigmate combination, Gomphonema turris Ehr. var. okamurae (Skv.) Fan et Bao.comb.nov., and two new varieties, Gomphonema turris Ehr. var. latum Fan et Wang. var. nov. and Gomphonema ac...The authors discribed a new polystigmate combination, Gomphonema turris Ehr. var. okamurae (Skv.) Fan et Bao.comb.nov., and two new varieties, Gomphonema turris Ehr. var. latum Fan et Wang. var. nov. and Gomphonema acuminatum var. obtusum Fan et Bao, var. nov., that were distinguished by frustule morphology under LM. The samples were collected from freshwater of Heilongjiang Province, China.展开更多
Breeding for wilt resistance and its theoretical basis are primarily responsible for increases in cotton yield and fiber quality. Breeding for immunity is the most efficient method in our struggle with infectious dise...Breeding for wilt resistance and its theoretical basis are primarily responsible for increases in cotton yield and fiber quality. Breeding for immunity is the most efficient method in our struggle with infectious diseases.展开更多
The Union for the Protection of New Varieties of Plants is abbreviated as UPOV. The Convention for the Protection of New Varieties of Plants has 1961/1972 Act ,1978 Act and 1991 Act. China is member of UPOV and its re...The Union for the Protection of New Varieties of Plants is abbreviated as UPOV. The Convention for the Protection of New Varieties of Plants has 1961/1972 Act ,1978 Act and 1991 Act. China is member of UPOV and its regulations of protection of new varieties of plants conform to 1978 Act. At present, there is big debate whether China accedes to 1991 Act. This paper mainly discussed advantages and possibilities that China accedes to 1991 Act, and concluded that it is highly desirable that China should consider acceding to 1991 Act in order to contribute to development of system on protection of new varieties of plants.展开更多
This paper studies farmers'privilege under the protection of new plant varieties,and points out that farmers'privilege is given to balance interests of plant breeders and relevant public.Based on this,it analy...This paper studies farmers'privilege under the protection of new plant varieties,and points out that farmers'privilege is given to balance interests of plant breeders and relevant public.Based on this,it analyzes shortcomings of farmers'privilege regulations,and puts forward that it is required to improve farmers'privilege regulations in subjects,objects and contents of rights on the basis of learning from advanced legislation experience of foreign countries,and build the interest sharing mechanism between breeders and farmers.展开更多
[ Objective] This study aimed to analyze the quality characteristics of the new middle-ripejapon/ca rico varieties in Jiangsu Province. [ Method] Four- teen new rice varieties included in the Middle-ripe Japon/ca Rice...[ Objective] This study aimed to analyze the quality characteristics of the new middle-ripejapon/ca rico varieties in Jiangsu Province. [ Method] Four- teen new rice varieties included in the Middle-ripe Japon/ca Rice Regional Test in 2011 were selected as the materials. Correlation analysis and cluster analysis were carried out on their quality traits. [Result] The rice cultivars possessed stable characteristics, which were difficult to be further improved. In addition, their quality traits had extremely complicated relationships, and the differences were significant between different groups. [ Conclusion] This study will provide reference for improving the quality traits of japonica rice.展开更多
Phenotypic screening of soybean germplasm suitable for high planting density is currently the most viable strategy to increase yield. Previous studies have shown that soybean varieties with dwarf features and a short ...Phenotypic screening of soybean germplasm suitable for high planting density is currently the most viable strategy to increase yield. Previous studies have shown that soybean varieties with dwarf features and a short petiole often exhibit a compact plant architecture which could improve yield through increased planting density, although previously reported short petiole accessions were ultimately not usable for breeding in practice. Here, we established a method to assess petiole length and identified an elite mutant line, M657, that exhibits high photosynthetic efficiency. The agronomic traits of M657 were evaluated under field conditions, and appeared to be stable for short petiole across seven locations in northern, Huang–Huai, and southern China from 2017 to 2018. Compared with the Jihuang 13 wild type, the mutant M657 was shorter in both petiole length and plant height, exhibited lower total area of leaf, seed weight per plant and 100-seed weight, but had an increased number of effective branches and the growth period was prolonged by 2–7 days. Using M657 as a parental line for crosses with four other elite lines, we obtained four lines with desirable plant architecture and yield traits, thus demonstrating the feasibility of adopting M657 in breeding programs for soybean cultivars of high density and high yield.展开更多
[Objective]The paper was to seek new varieties of high quality conventional rice suitable for Yangjiang area,Guangdong Province,China.[Method]In 2017,introduction test was performed with seven new varieties of high-qu...[Objective]The paper was to seek new varieties of high quality conventional rice suitable for Yangjiang area,Guangdong Province,China.[Method]In 2017,introduction test was performed with seven new varieties of high-quality conventional rice approved by Guangdong Province in recent years,including Huangsilizhan,Guangjingruanzhan,Heliyouzhan,Huahang 48,Limiaozhan,Lvyinzhan,Yuehesimiao,to investigate the main agronomic traits and yield of all varieties tested.[Result]The comprehensive performance of Lvyinzhan,Guangjingruanzhan,Limiaozhan and Huahang 48 was outstanding,showing the characters of good yield,excellent rice quality and strong stress resistance.[Conclusion]Lvyinzhan,Guangjingruanzhan,Limiaozhan and Huahang 48 are suitable for large-scale demonstration and promotion in Yangjiang area.展开更多
After 8 yrs lab experiments and field tests, an advanced technique for identifying varieties resistance to rice blast was developed by a research group in Plant Protection Institute, Zhejiang Academy of Agricultural S...After 8 yrs lab experiments and field tests, an advanced technique for identifying varieties resistance to rice blast was developed by a research group in Plant Protection Institute, Zhejiang Academy of Agricultural Sciences. With this technique, the inoculum was prepared on a maize-rice-straw-agar media which was suitable for sporulation of most rice blast pathogen isolates. To avoid possible virulence variation during the vegetative growth, the conidia were harvested and preserved for later different sets of resistance evaluation tests. A simple and precise new method—smear inoculation was used for infection. The experi-展开更多
基金the results obtained from phases Ⅲ , Ⅳ, Ⅴ, and Ⅵ of the IRRI – Japan Collaborative Research Project, which was supported by the Ministry of Foreign Affairs and the Ministry of Agriculture, Forestry and Fisheries of Japan
文摘To enhance the yield potential of an elite indica rice cultivar,an introgression(BC_3-derived) line of IR64,YTH288,was developed using a new-plant-type cultivar,IR66215-44-2-3,as a donor parent.YTH288 has agronomically valuable characteristics such as large panicles,few unproductive tillers,and large leaves inherited from NPT.To identify the genetic basis of these traits,we used 167 F_2 plants derived from a cross between IR64 and YTH288 to conduct QTL analysis for five agronomic traits:days to heading(DTH),culm length(CL),flag leaf length(FLL),flag leaf width(FLW),and filled spikelet number per panicle(FSN).Six putative QTL were detected:four on chromosome4(for CL,FLL,FLW,and FSN) and two on chromosome 2(for DTH and FLL).All QTL with the IR66215-44-2-3 allele,except that for FLL on chromosome 2,had positive effects on each trait.To confirm the effects of these putative QTL,we developed NILs with the IR64 genetic background by marker-assisted selection.We observed significant differences in several agronomic traits between IR64 and NILs that carried these QTL on chromosomes2 and 4.Additionally,four IR64-NILs carrying chromosomal segments derived from different NPT varieties on the long arm of chromosome 4 exhibited similar pleiotropic To enhance the yield potential of an elite indica rice cultivar, an introgression(BC3-derived) line of IR64, YTH288, was developed using a new-plant-type cultivar,IR66215-44-2-3, as a donor parent. YTH288 has agronomically valuable characteristics such as large panicles, few unproductive tillers, and large leaves inherited from NPT.To identify the genetic basis of these traits, we used 167 F2 plants derived from a cross between IR64 and YTH288 to conduct QTL analysis for five agronomic traits: days to heading(DTH), culm length(CL), flag leaf length(FLL), flag leaf width(FLW), and filled spikelet number per panicle(FSN). Six putative QTL were detected: four on chromosome4(for CL, FLL, FLW, and FSN) and two on chromosome 2(for DTH and FLL). All QTL with the IR66215-44-2-3 allele, except that for FLL on chromosome 2, had positive effects on each trait. To confirm the effects of these putative QTL, we developed NILs with the IR64 genetic background by marker-assisted selection. We observed significant differences in several agronomic traits between IR64 and NILs that carried these QTL on chromosomes2 and 4. Additionally, four IR64-NILs carrying chromosomal segments derived from different NPT varieties on the long arm of chromosome 4 exhibited similar pleiotropiceffects for unique agronomic traits. These NILs can be used as research materials for studying each trait and as breeding materials for yield improvement of indica rice cultivars.
文摘Protein sources in the diet of people living in semi-arid land of Kenya are lacking and if available it is costly to them. In terms of safe food and a healthy food supply, cowpeas (Vigna unguiculata.) are a significant source of protein, carbohydrates, and minerals especially for poor populations in the Kenya, it is said to be poor man’s meat. The aim of this study was to determine nutritional composition of newly bred ten cowpea lines and five varieties commonly grown in Eastern Kenya of Kitui, Machakos and Makueni counties to understand their potential utilization in curbing rising food and nutrition insecurity in arid and semi-arid lands ASALs and in any other food applications in Kenya. The experiment was arranged in Completely Randomized Design (CRD) whereby proximate composition and minerals were determined using standard AOAC and AACC methods and technological characteristics checked using modified methods used by other researchers. Collected data were subjected to Analysis of Variance (ANOVA) using SAS (2006) version 9.1, mean separation was done using Tukey’s Honestly Significant Difference (HSD) method at 5% level of significance. Cowpeas composition ranged from 12.28% - 13.35% for moisture content, 49.37% - 55.74% for total carbohydrates, 2.99% - 3.34% for crude ash, 0.13% - 0.81% for crude lipids, 23.37% - 29.70% for crude protein and 1.40% - 4.34% for crude fibers. Cowpea samples recorded highest percentage of essential amino acids (60.71%) and non-essential amino acids (39.29%). Minerals ranged from 1.97 - 2.69 mg/100g for calcium, 3.23 - 3.90 mg/100g for magnesium, 205.53 - 223.30 mg/100g for sodium, 0.80 - 1.23 mg/100g for zinc, 1071.15 - 1152.62 mg/100g for potassium and 0.62 - 1.06 mg/100g for phosphorus. For technological properties, lines absorbed water equivalent to their weights and they were comparable to varieties grown in the region. From the results it showed that cowpea line IT97K-1042-3, TEXAN PINKIYE, TX123, IT85F-867-5, IT82D-889-1 and IT82D-889 have desirable attributes such as high crude protein contents, good water absorption capacities and volumetric expansion. They compared well with existing K80 variety. These cowpea lines could possibly be bred and combined into a single cowpea line and further improved by breeders to have other good properties such as higher levels of water absorption during soaking hence reduced cooking times. Therefore, this work has shown that cowpea lines developed can be used as food security crop, industrial food applications and enriching foods of low protein like in complementary foods for healthy food supply in Eastern Kenya.
基金by Undergraduate Innovation and Entrepreneurship Training Program of Anhui Province(S202312216042)Natural Science Key Research Project of Colleges and Universities in Anhui Province(2023AH051816)General Teaching Research Project of Anhui Province(2022jyxm665).
文摘An analytic hierarchy process(AHP)was employed to assess the applicability of 18 new and superior varieties of flowers in Hefei City flower border applications.A total of 12 indicators were selected from three distinct aspects of adaptability,ornamental characteristics and use traits,in order to establish a comprehensive evaluation model.The results demonstrate that grade I(J≥2.685)exhibits excellent application value,encompassing six species of plants,such asHydrangeamacrophylla‘Endless Summer’;grade II(2.684≤J≤2.420)is also of notable application value,encompassing five species of plants,such asCallistemonrigidus;grade III(2.419≤J≤2.615)is of average application value,including five species of plants,such asCrocosmiacrocosmiflora;grade IV(J≤2.16)is of relatively poor application value.The evaluation results may be utilized as a theoretical reference for the promotion of new and superior varieties in the flower border of Hefei.
文摘Ⅱ-you 92: a new hybrid rice for late seasonⅡ-you 92 is an indica hybrid rice that was developed by Jinhua Agri Res Inst, Zhejiang Province.Ⅱ-you 92 has a suitable growth duration of 122-125 d as double-cropping late season rice, 3-4 dshorter than that of Shanyou 64 and 6-7 d shorter than Shanyou 10. It has a compact plant type, high tiller-ing ability and plant height of 85-90 cn. A number of 3.4-3.6 million effective panicles per ha can be
文摘Qixiuzhan 3:a new high-yielding, good-quality indica rice varietyBy crossing Qishanzhan with Xinxiu 299, a new variety Qixiuzhan 3 was developed by the Rice Res Inst ofGuangdong Acad of Agri Sci.Being planted in the early season, this variety belongs to mid-late-maturity rice, with the same ma-
文摘The authors discribed a new polystigmate combination, Gomphonema turris Ehr. var. okamurae (Skv.) Fan et Bao.comb.nov., and two new varieties, Gomphonema turris Ehr. var. latum Fan et Wang. var. nov. and Gomphonema acuminatum var. obtusum Fan et Bao, var. nov., that were distinguished by frustule morphology under LM. The samples were collected from freshwater of Heilongjiang Province, China.
文摘Breeding for wilt resistance and its theoretical basis are primarily responsible for increases in cotton yield and fiber quality. Breeding for immunity is the most efficient method in our struggle with infectious diseases.
文摘The Union for the Protection of New Varieties of Plants is abbreviated as UPOV. The Convention for the Protection of New Varieties of Plants has 1961/1972 Act ,1978 Act and 1991 Act. China is member of UPOV and its regulations of protection of new varieties of plants conform to 1978 Act. At present, there is big debate whether China accedes to 1991 Act. This paper mainly discussed advantages and possibilities that China accedes to 1991 Act, and concluded that it is highly desirable that China should consider acceding to 1991 Act in order to contribute to development of system on protection of new varieties of plants.
文摘This paper studies farmers'privilege under the protection of new plant varieties,and points out that farmers'privilege is given to balance interests of plant breeders and relevant public.Based on this,it analyzes shortcomings of farmers'privilege regulations,and puts forward that it is required to improve farmers'privilege regulations in subjects,objects and contents of rights on the basis of learning from advanced legislation experience of foreign countries,and build the interest sharing mechanism between breeders and farmers.
基金Supported by the Project for Science and Technology Transformation in Jiangsu Province(BA2008083)
文摘[ Objective] This study aimed to analyze the quality characteristics of the new middle-ripejapon/ca rico varieties in Jiangsu Province. [ Method] Four- teen new rice varieties included in the Middle-ripe Japon/ca Rice Regional Test in 2011 were selected as the materials. Correlation analysis and cluster analysis were carried out on their quality traits. [Result] The rice cultivars possessed stable characteristics, which were difficult to be further improved. In addition, their quality traits had extremely complicated relationships, and the differences were significant between different groups. [ Conclusion] This study will provide reference for improving the quality traits of japonica rice.
基金funded by the National Natural Science Foundation of China(31271753)the Agricultural Science and Technology Innovation Program(ASTIP)of Chinese Academy of Agricultural Sciences(CAASZDRW202003-1)。
文摘Phenotypic screening of soybean germplasm suitable for high planting density is currently the most viable strategy to increase yield. Previous studies have shown that soybean varieties with dwarf features and a short petiole often exhibit a compact plant architecture which could improve yield through increased planting density, although previously reported short petiole accessions were ultimately not usable for breeding in practice. Here, we established a method to assess petiole length and identified an elite mutant line, M657, that exhibits high photosynthetic efficiency. The agronomic traits of M657 were evaluated under field conditions, and appeared to be stable for short petiole across seven locations in northern, Huang–Huai, and southern China from 2017 to 2018. Compared with the Jihuang 13 wild type, the mutant M657 was shorter in both petiole length and plant height, exhibited lower total area of leaf, seed weight per plant and 100-seed weight, but had an increased number of effective branches and the growth period was prolonged by 2–7 days. Using M657 as a parental line for crosses with four other elite lines, we obtained four lines with desirable plant architecture and yield traits, thus demonstrating the feasibility of adopting M657 in breeding programs for soybean cultivars of high density and high yield.
文摘[Objective]The paper was to seek new varieties of high quality conventional rice suitable for Yangjiang area,Guangdong Province,China.[Method]In 2017,introduction test was performed with seven new varieties of high-quality conventional rice approved by Guangdong Province in recent years,including Huangsilizhan,Guangjingruanzhan,Heliyouzhan,Huahang 48,Limiaozhan,Lvyinzhan,Yuehesimiao,to investigate the main agronomic traits and yield of all varieties tested.[Result]The comprehensive performance of Lvyinzhan,Guangjingruanzhan,Limiaozhan and Huahang 48 was outstanding,showing the characters of good yield,excellent rice quality and strong stress resistance.[Conclusion]Lvyinzhan,Guangjingruanzhan,Limiaozhan and Huahang 48 are suitable for large-scale demonstration and promotion in Yangjiang area.
文摘After 8 yrs lab experiments and field tests, an advanced technique for identifying varieties resistance to rice blast was developed by a research group in Plant Protection Institute, Zhejiang Academy of Agricultural Sciences. With this technique, the inoculum was prepared on a maize-rice-straw-agar media which was suitable for sporulation of most rice blast pathogen isolates. To avoid possible virulence variation during the vegetative growth, the conidia were harvested and preserved for later different sets of resistance evaluation tests. A simple and precise new method—smear inoculation was used for infection. The experi-