Characterization of QTL for unique agronomic traits of new-plant-type rice varieties using introgression lines of IR64

To enhance the yield potential of an elite indica rice cultivar,an introgression(BC_3-derived) line of IR64,YTH288,was developed using a new-plant-type cultivar,IR66215-44-2-3,as a donor parent.YTH288 has agronomically valuable characteristics such as large panicles,few unproductive tillers,and large leaves inherited from NPT.To identify the genetic basis of these traits,we used 167 F_2 plants derived from a cross between IR64 and YTH288 to conduct QTL analysis for five agronomic traits:days to heading(DTH),culm length(CL),flag leaf length(FLL),flag leaf width(FLW),and filled spikelet number per panicle(FSN).Six putative QTL were detected:four on chromosome4(for CL,FLL,FLW,and FSN) and two on chromosome 2(for DTH and FLL).All QTL with the IR66215-44-2-3 allele,except that for FLL on chromosome 2,had positive effects on each trait.To confirm the effects of these putative QTL,we developed NILs with the IR64 genetic background by marker-assisted selection.We observed significant differences in several agronomic traits between IR64 and NILs that carried these QTL on chromosomes2 and 4.Additionally,four IR64-NILs carrying chromosomal segments derived from different NPT varieties on the long arm of chromosome 4 exhibited similar pleiotropic To enhance the yield potential of an elite indica rice cultivar, an introgression(BC3-derived) line of IR64, YTH288, was developed using a new-plant-type cultivar,IR66215-44-2-3, as a donor parent. YTH288 has agronomically valuable characteristics such as large panicles, few unproductive tillers, and large leaves inherited from NPT.To identify the genetic basis of these traits, we used 167 F2 plants derived from a cross between IR64 and YTH288 to conduct QTL analysis for five agronomic traits: days to heading(DTH), culm length(CL), flag leaf length(FLL), flag leaf width(FLW), and filled spikelet number per panicle(FSN). Six putative QTL were detected: four on chromosome4(for CL, FLL, FLW, and FSN) and two on chromosome 2(for DTH and FLL). All QTL with the IR66215-44-2-3 allele, except that for FLL on chromosome 2, had positive effects on each trait. To confirm the effects of these putative QTL, we developed NILs with the IR64 genetic background by marker-assisted selection. We observed significant differences in several agronomic traits between IR64 and NILs that carried these QTL on chromosomes2 and 4. Additionally, four IR64-NILs carrying chromosomal segments derived from different NPT varieties on the long arm of chromosome 4 exhibited similar pleiotropiceffects for unique agronomic traits. These NILs can be used as research materials for studying each trait and as breeding materials for yield improvement of indica rice cultivars.

Nutritional and Technological Characteristics of New Cowpea (<i>Vigna unguiculata</i>) Lines and Varieties Grown in Eastern Kenya

Protein sources in the diet of people living in semi-arid land of Kenya are lacking and if available it is costly to them. In terms of safe food and a healthy food supply, cowpeas (Vigna unguiculata.) are a significant source of protein, carbohydrates, and minerals especially for poor populations in the Kenya, it is said to be poor man’s meat. The aim of this study was to determine nutritional composition of newly bred ten cowpea lines and five varieties commonly grown in Eastern Kenya of Kitui, Machakos and Makueni counties to understand their potential utilization in curbing rising food and nutrition insecurity in arid and semi-arid lands ASALs and in any other food applications in Kenya. The experiment was arranged in Completely Randomized Design (CRD) whereby proximate composition and minerals were determined using standard AOAC and AACC methods and technological characteristics checked using modified methods used by other researchers. Collected data were subjected to Analysis of Variance (ANOVA) using SAS (2006) version 9.1, mean separation was done using Tukey’s Honestly Significant Difference (HSD) method at 5% level of significance. Cowpeas composition ranged from 12.28% - 13.35% for moisture content, 49.37% - 55.74% for total carbohydrates, 2.99% - 3.34% for crude ash, 0.13% - 0.81% for crude lipids, 23.37% - 29.70% for crude protein and 1.40% - 4.34% for crude fibers. Cowpea samples recorded highest percentage of essential amino acids (60.71%) and non-essential amino acids (39.29%). Minerals ranged from 1.97 - 2.69 mg/100g for calcium, 3.23 - 3.90 mg/100g for magnesium, 205.53 - 223.30 mg/100g for sodium, 0.80 - 1.23 mg/100g for zinc, 1071.15 - 1152.62 mg/100g for potassium and 0.62 - 1.06 mg/100g for phosphorus. For technological properties, lines absorbed water equivalent to their weights and they were comparable to varieties grown in the region. From the results it showed that cowpea line IT97K-1042-3, TEXAN PINKIYE, TX123, IT85F-867-5, IT82D-889-1 and IT82D-889 have desirable attributes such as high crude protein contents, good water absorption capacities and volumetric expansion. They compared well with existing K80 variety. These cowpea lines could possibly be bred and combined into a single cowpea line and further improved by breeders to have other good properties such as higher levels of water absorption during soaking hence reduced cooking times. Therefore, this work has shown that cowpea lines developed can be used as food security crop, industrial food applications and enriching foods of low protein like in complementary foods for healthy food supply in Eastern Kenya.

Comprehensive Evaluation of Flower Border Application Value of New and Superior Plants in Hefei Area

An analytic hierarchy process(AHP)was employed to assess the applicability of 18 new and superior varieties of flowers in Hefei City flower border applications.A total of 12 indicators were selected from three distinct aspects of adaptability,ornamental characteristics and use traits,in order to establish a comprehensive evaluation model.The results demonstrate that grade I(J≥2.685)exhibits excellent application value,encompassing six species of plants,such asHydrangeamacrophylla‘Endless Summer’;grade II(2.684≤J≤2.420)is also of notable application value,encompassing five species of plants,such asCallistemonrigidus;grade III(2.419≤J≤2.615)is of average application value,including five species of plants,such asCrocosmiacrocosmiflora;grade IV(J≤2.16)is of relatively poor application value.The evaluation results may be utilized as a theoretical reference for the promotion of new and superior varieties in the flower border of Hefei.

NEW VARIETIES AND LINES

Ⅱ-you 92: a new hybrid rice for late seasonⅡ-you 92 is an indica hybrid rice that was developed by Jinhua Agri Res Inst, Zhejiang Province.Ⅱ-you 92 has a suitable growth duration of 122-125 d as double-cropping late season rice, 3-4 dshorter than that of Shanyou 64 and 6-7 d shorter than Shanyou 10. It has a compact plant type, high tiller-ing ability and plant height of 85-90 cn. A number of 3.4-3.6 million effective panicles per ha can be

NEW VARIETIES AND LINES

Qixiuzhan 3:a new high-yielding, good-quality indica rice varietyBy crossing Qishanzhan with Xinxiu 299, a new variety Qixiuzhan 3 was developed by the Rice Res Inst ofGuangdong Acad of Agri Sci.Being planted in the early season, this variety belongs to mid-late-maturity rice, with the same ma-

A New Combination and Two Varieties of Polystigmate Gomphonema (Gomphonemaceae Bacillariophyta) from Heilongjiang Province, China

The authors discribed a new polystigmate combination, Gomphonema turris Ehr. var. okamurae (Skv.) Fan et Bao.comb.nov., and two new varieties, Gomphonema turris Ehr. var. latum Fan et Wang. var. nov. and Gomphonema acuminatum var. obtusum Fan et Bao, var. nov., that were distinguished by frustule morphology under LM. The samples were collected from freshwater of Heilongjiang Province, China.

Correlation of Inhibitor Proteinase in Varieties and Lines of Cotton(Gossypium hirsutum L.) to Different Geographic Population of Verticillium dathliae Klebahn

Breeding for wilt resistance and its theoretical basis are primarily responsible for increases in cotton yield and fiber quality. Breeding for immunity is the most efficient method in our struggle with infectious diseases.

Impact of Implementing 1991 Act of the International Convention for the Protection of New Varieties of Plants in China

The Union for the Protection of New Varieties of Plants is abbreviated as UPOV. The Convention for the Protection of New Varieties of Plants has 1961/1972 Act ,1978 Act and 1991 Act. China is member of UPOV and its regulations of protection of new varieties of plants conform to 1978 Act. At present, there is big debate whether China accedes to 1991 Act. This paper mainly discussed advantages and possibilities that China accedes to 1991 Act, and concluded that it is highly desirable that China should consider acceding to 1991 Act in order to contribute to development of system on protection of new varieties of plants.

Farmers' Privilege under the Protection of New Plant Varieties

This paper studies farmers'privilege under the protection of new plant varieties,and points out that farmers'privilege is given to balance interests of plant breeders and relevant public.Based on this,it analyzes shortcomings of farmers'privilege regulations,and puts forward that it is required to improve farmers'privilege regulations in subjects,objects and contents of rights on the basis of learning from advanced legislation experience of foreign countries,and build the interest sharing mechanism between breeders and farmers.

Correlation and Cluster Analysis on the Quality Characters of New Middle-ripe Japonica Rice Varieties in Jiangsu Province

[ Objective] This study aimed to analyze the quality characteristics of the new middle-ripejapon/ca rico varieties in Jiangsu Province. [ Method] Four- teen new rice varieties included in the Middle-ripe Japon/ca Rice Regional Test in 2011 were selected as the materials. Correlation analysis and cluster analysis were carried out on their quality traits. [Result] The rice cultivars possessed stable characteristics, which were difficult to be further improved. In addition, their quality traits had extremely complicated relationships, and the differences were significant between different groups. [ Conclusion] This study will provide reference for improving the quality traits of japonica rice.

Characterization of the petiole length in soybean compact architecture mutant M657 and the breeding of new lines

Phenotypic screening of soybean germplasm suitable for high planting density is currently the most viable strategy to increase yield. Previous studies have shown that soybean varieties with dwarf features and a short petiole often exhibit a compact plant architecture which could improve yield through increased planting density, although previously reported short petiole accessions were ultimately not usable for breeding in practice. Here, we established a method to assess petiole length and identified an elite mutant line, M657, that exhibits high photosynthetic efficiency. The agronomic traits of M657 were evaluated under field conditions, and appeared to be stable for short petiole across seven locations in northern, Huang–Huai, and southern China from 2017 to 2018. Compared with the Jihuang 13 wild type, the mutant M657 was shorter in both petiole length and plant height, exhibited lower total area of leaf, seed weight per plant and 100-seed weight, but had an increased number of effective branches and the growth period was prolonged by 2–7 days. Using M657 as a parental line for crosses with four other elite lines, we obtained four lines with desirable plant architecture and yield traits, thus demonstrating the feasibility of adopting M657 in breeding programs for soybean cultivars of high density and high yield.

Study on Introduction Test of New Varieties of High Quality Conventional Rice in Yangjiang City, China

[Objective]The paper was to seek new varieties of high quality conventional rice suitable for Yangjiang area,Guangdong Province,China.[Method]In 2017,introduction test was performed with seven new varieties of high-quality conventional rice approved by Guangdong Province in recent years,including Huangsilizhan,Guangjingruanzhan,Heliyouzhan,Huahang 48,Limiaozhan,Lvyinzhan,Yuehesimiao,to investigate the main agronomic traits and yield of all varieties tested.[Result]The comprehensive performance of Lvyinzhan,Guangjingruanzhan,Limiaozhan and Huahang 48 was outstanding,showing the characters of good yield,excellent rice quality and strong stress resistance.[Conclusion]Lvyinzhan,Guangjingruanzhan,Limiaozhan and Huahang 48 are suitable for large-scale demonstration and promotion in Yangjiang area.

Cultivar Mixture Cooperation(CMC)of rice varieties(lines) with different blast resistant gene

We studied Cultivar Mixture Cooperation(CMC)of rice varieties(lines)by screening mixture ofrice varieties which possess different genes,1983-1996.

New technique for identifying varieties resistance to rice blast

After 8 yrs lab experiments and field tests, an advanced technique for identifying varieties resistance to rice blast was developed by a research group in Plant Protection Institute, Zhejiang Academy of Agricultural Sciences. With this technique, the inoculum was prepared on a maize-rice-straw-agar media which was suitable for sporulation of most rice blast pathogen isolates. To avoid possible virulence variation during the vegetative growth, the conidia were harvested and preserved for later different sets of resistance evaluation tests. A simple and precise new method—smear inoculation was used for infection. The experi-

基于主成分和聚类分析7个深绿线椒品种的商品品质和产量评价

【目的】探明深绿线椒品种的商品品质和产量差异,为贵州辣椒产业品种结构调整和优势区域布局提供科学依据。【方法】以7个深绿线椒品种(长辣七号、湘辣17号、卓椒七号、卓椒八号、香辣八号、红冠303、泰丰五号)为研究对象,采用SPSS 26.0进行变异系数分析和主成分分析,Origin 2022进行相关性和聚类分析,对其商品品质与产量进行综合评价。【结果】7个深绿线椒品种的商品品质及产量性状变异系数为6.91%~28.17%,其中,以果形指数变异系数最小,单果鲜重的变异系数最大;单果鲜重与果纵径、鲜椒产量与干物质含量分别呈极显著相关性,果纵径与果横径、单果鲜重与果横径、干椒产量与单株挂果数、单株挂果数与果纵径、单株挂果数与单果鲜重、单果鲜重与干物质含量分别呈显著相关性。基于深绿线椒品种的9个性状,通过主成分分析建立综合表现评价模型,得出品种卓椒七号的综合表现最佳,其产量最高,达2410 kg/667m^(2)。聚类分析将7个线椒品种分为适合鲜加工(长辣七号、红冠303、香辣八号和湘辣17号)、既适合鲜加工又适合干加工(泰丰五号和卓椒八号)及适合绿熟期采收上市(卓椒七号)3类。【结论】7个深绿线椒品种的多样性丰富,单果鲜重、果横径、果纵径、单株挂果数是构成鲜椒产量的主要因素,单株挂果数是构成干椒产量的主要因素;卓椒七号鲜椒产量最高,适合在绿熟期采收上市;卓椒八号果形和果肉厚适中,鲜椒和干椒产量中上,是理想的加工型辣椒品种。

酿酒葡萄新品种豫酿紫玉的选育

豫酿紫玉葡萄是以梅鹿辄(Vitis vinifera‘Merlot’♀)和LN-33(Vitis vinifera‘LN-33’♂)为亲本经人工杂交培育而成的中早熟酿酒葡萄新品种。果穗圆锥形,偶有副穗,果粒紧凑,平均单穗质量252.70 g;果粒中等,大小均衡,平均单粒质量1.53 g;果粒近圆形,紫黑色,着色均匀,果粉中等厚,果皮中等厚,有肉囊,汁多,每果种子1~2粒;果汁无色,压榨葡萄汁呈宝石红色。成熟果可溶性固形物含量(w,后同)为21.6%,可溶性糖含量为17.46%,总酸含量为0.78%,维生素C含量为9.63 mg·100 g-1,单宁含量为4680 mg·kg-1;果实酸甜适口,果肉具有李子果香味。在郑州地区栽培,浆果于7月上旬开始着色,7月下旬始熟,8月上旬充分成熟,果实发育期为82 d;萌芽率高,生长健壮。酿酒样品酒精度、干浸出物含量、可滴定酸含量、pH值、二氧化硫残留量均在酿造干型红葡萄酒的国标范围内;酿造的干型红葡萄酒风味正,颜色佳。该品种耐寒性强,耐干旱,抗病害,丰产性好。适合在河南葡萄生态适生区和与其相似的其他葡萄生态适宜区栽培。

2003~2021年新疆农业植物新品种申请量与授权量对比分析

【目的】分析新疆农业植物新品种权申请和授权现状并比较。【方法】收集和汇总2003~2021年间新疆农业植物新品种权申请授权数据,比较分析新品种权申请量与授权量变化。【结果】2021年12月底,新疆农业植物新品种累计公告申请量达470件,公告授权总量达154件,授权申请比为0.33。2003~2021年间新疆主要农作物申请品种审定718件,其中申请小麦品种审定105件,占14.62%;申请玉米品种审定253件,占35.24%;申请水稻品种审定59件,占8.22%;申请棉花品种审定259件,占36.07%;申请大豆品种审定42件,占5.85%;申请品种权保护的数量远不如申请审定的数量。【结论】种子企业和农业科研院所是新疆农业植物新品种申请的主要机构,2003~2021年,两者申请量占新疆总申请量的95.94%。其中企业申请量为282件,占60%;新疆植物新品种权按申请量排名,在全国31个省(区、市)中位居第24位,按授权量排名,在全国31个省(区、市)中位居第26位,按主要农作物审定数量排名,在全国31个省(区、市)中位居第21位。

皱木耳‘中菌皱木耳1号’选育

将采自云南省临沧市永德县大雪山乡大平掌村壳斗科(Fagaceae)倒腐木上的野生子实体鉴定为皱木耳(Auricularia delicata),经过组织分离、初筛,以菌株YD-1为对照,通过复筛获得最优菌株,通过拮抗实验、构建系统发育树比较菌株间的差异,通过中试和示范栽培比较菌株间的生物学效率。结果表明:初筛选出菌丝生长旺盛、菌丝生长速度较快和生物学效率较高的分离物ZJZME001、ZJZME002和ZJZME005,复筛时对照菌株YD-1的子实体颜色为黄褐色,分离物ZJZME001、ZJZME002和ZJZME005的子实体颜色为浅黄色,获得最优菌株ZJZME001,与菌株YD-1形成明显的拮抗线;通过系统发育树分析表明菌株ZJZME001为皱木耳,其中试和示范栽培的生物学效率均显著高于对照菌株YD-1,达120%以上;与对照菌株YD-1相比,菌株ZJZME001的子实体的锌、铁、钙、硒、蛋白质和维生素B1含量较高,氨基酸总量也较高,但粗纤维含量较低。2023年9月25日,菌株ZJZME001通过云南省非主要农作物品种鉴定,定名为‘中菌皱木耳1号’。

高油亚比抗病红皮花生新品种莆花60选育及栽培技术要点

高油亚比抗病红皮花生新品种莆花60是以红皮花生湛红2号为母本、泉花10为父本,通过人工有性杂交、多点多代定向选育而成。对莆花60生产力、农艺性状、品质性状和抗病性等进行研究,结果表明:莆花60荚果和籽仁产量高,种皮为深红色,两年荚果平均产量3982.3 kg/hm^(2),比对照泉花7号增产10.79%;籽仁平均产量2726.0 kg/hm^(2),比泉花7号增产8.36%;该品种表现高产、稳产,适应性广,抗涝性、抗旱性、抗倒性强,中抗青枯病,高抗锈病及叶斑病,油亚比平均值为2.36,含油量较高,商品性较佳,2022年12月通过国家非主要农作物品种登记[GPD花生(2022)350212],适宜在福建各地推广种植。

马铃薯新品种‘牡育薯1号’的选育

‘牡育薯1号’是以‘GP2-12’为母本、‘NS78-17’为父本,通过有性杂交选育而成的鲜食型马铃薯新品种。2022年通过农业农村部非主要农作物品种登记,登记编号:GPD马铃薯(2022)230011。‘牡育薯1号’生育期77 d,植株半直立,平均株高约68.8 cm,生长势强,茎绿色,叶绿色,花冠浅紫色。天然结实性中等。块茎卵圆形,黄色皮、深黄色肉,芽眼深度中等。平均单株结薯数6.1个,平均单薯重171 g,商品薯率79.70%。干物质含量19.00 g/100 g,淀粉含量13.30 g/100 g,块茎粗蛋白含量2.04 g/100 g,维生素C含量14.20 mg/100 g,还原糖含量1.10%。2年区域试验中,较对照‘克新13号’平均总增产9.16%。中抗晚疫病、中抗马铃薯X病毒(Potato virus X,PVX)和马铃薯Y病毒(Potato virus Y,PVY)。‘牡育薯1号’适宜在黑龙江省春季种植。