[Objective] The paper was to provide a basis for scientific prevention and control of pigeon Newcastle disease（ND）.[Method] The HN gene of eight pigeon NDV strains isolated from different pigeon farms in Guangxi wer...[Objective] The paper was to provide a basis for scientific prevention and control of pigeon Newcastle disease（ND）.[Method] The HN gene of eight pigeon NDV strains isolated from different pigeon farms in Guangxi were amplified by RT-PCR,sequenced and analyzed.The molecular evolution characteristics of HN gene of pigeon NDV isolates in Guangxi was discussed.[Result] The nucleotide sequence length of HN gene of the eight NDV isolates was 1 716 bp,encoding 571 amino acids.They belonged to virulent group C,and the gene length characteristic of HN gene accorded with virulent strain.Analysis of nucleotide homologies indicated that the eight NDV isolates shared higher homology with genotype VIb,ranging from 90.4% to 99.5%.Phylogenetic tree analysis demonstrated that the genetic relationship between the eight NDV strains in Gangxi and the NDV isolates from Guangxi,Guangdong,Jilin,Liaoning,Yunnan and Heilongjiang during 2011 and 2013 was close.They were located in the same cladogram branch.[Conclusion] We assume that the eight pigeon NDV isolates in Guangxi all belong to the gene class II genotype VI b NDV.展开更多
The influence of antibody immune selective pressure on Newcastle disease virus (NDV) HN and F gene mutations was studied in cell cultures.NDV field strain TZ060107 was inoculated into chicken embryo fibroblast cells a...The influence of antibody immune selective pressure on Newcastle disease virus (NDV) HN and F gene mutations was studied in cell cultures.NDV field strain TZ060107 was inoculated into chicken embryo fibroblast cells and continuously passaged with (group A) or without (group B) anti-NDV monospecific serum.Each group contained three independent passage series.HN and F genes were amplified and sequenced for the 10th,20th,30th,40th and 50th generations of each serial passage,and compared with the original strain.The results demonstrated that increased HN gene mutations were observed in group A with the antibody than in group B without the antibody.The nonsynonymous (NS) to synonymous (S) mutations ratio was 6 for group A,significantly higher than 3.4 in group B.In group A with the antibody,there were five stable NS mutations in HN gene,three of which (related to aa#353,521 and 568) were related to known epitopes.There were two stable NS mutations in F gene in group A,but no stable NS mutations in group B.The NS/S ratios of F gene were less than 2.5 for both groups A and B.Our results suggested that the antibody strongly influenced HN gene mutations,while the F gene was less influenced by the same antibody.展开更多
基金Supported by Science and Technology Major Projects of Guangxi Province(GKAA17204057)Systematic Research Project of Guangxi Key Laboratory of Veterinary Biotechnology(14-045-31-A-5)Fundamental Research Fund of Guangxi Veterinary Research Institute(GKZX 2016-2)
文摘[Objective] The paper was to provide a basis for scientific prevention and control of pigeon Newcastle disease（ND）.[Method] The HN gene of eight pigeon NDV strains isolated from different pigeon farms in Guangxi were amplified by RT-PCR,sequenced and analyzed.The molecular evolution characteristics of HN gene of pigeon NDV isolates in Guangxi was discussed.[Result] The nucleotide sequence length of HN gene of the eight NDV isolates was 1 716 bp,encoding 571 amino acids.They belonged to virulent group C,and the gene length characteristic of HN gene accorded with virulent strain.Analysis of nucleotide homologies indicated that the eight NDV isolates shared higher homology with genotype VIb,ranging from 90.4% to 99.5%.Phylogenetic tree analysis demonstrated that the genetic relationship between the eight NDV strains in Gangxi and the NDV isolates from Guangxi,Guangdong,Jilin,Liaoning,Yunnan and Heilongjiang during 2011 and 2013 was close.They were located in the same cladogram branch.[Conclusion] We assume that the eight pigeon NDV isolates in Guangxi all belong to the gene class II genotype VI b NDV.
基金supported by the Shandong Province Application Technology Innovation Project on Agriculture during 2007 and 2008
文摘The influence of antibody immune selective pressure on Newcastle disease virus (NDV) HN and F gene mutations was studied in cell cultures.NDV field strain TZ060107 was inoculated into chicken embryo fibroblast cells and continuously passaged with (group A) or without (group B) anti-NDV monospecific serum.Each group contained three independent passage series.HN and F genes were amplified and sequenced for the 10th,20th,30th,40th and 50th generations of each serial passage,and compared with the original strain.The results demonstrated that increased HN gene mutations were observed in group A with the antibody than in group B without the antibody.The nonsynonymous (NS) to synonymous (S) mutations ratio was 6 for group A,significantly higher than 3.4 in group B.In group A with the antibody,there were five stable NS mutations in HN gene,three of which (related to aa#353,521 and 568) were related to known epitopes.There were two stable NS mutations in F gene in group A,but no stable NS mutations in group B.The NS/S ratios of F gene were less than 2.5 for both groups A and B.Our results suggested that the antibody strongly influenced HN gene mutations,while the F gene was less influenced by the same antibody.
