CA IX-targeted Ag_(2)S quantum dots bioprobe for NIR-II imaging-guided hypoxia tumor chemo-photothermal therapy

Hypoxia is the common characteristic of almost all solid tumors,which prevents therapeutic drugs from reaching the tumors.Therefore,the development of new targeted agents for the accurate diagnosis of hypoxia tumors is widely concerned.As carbonic anhydrase IX(CA IX)is abundantly distributed on the hypoxia tumor cells,it is considered as a potential tumor biomarker.4-(2-Aminoethyl)benzenesulfonamide(ABS)as a CA IX inhibitor has inherent inhibitory activity and good targeting effect.In this study,Ag_(2)S quantum dots(QDs)were used as the carrier to prepare a novel diagnostic and therapeutic bioprobe(Ag_(2)S@polyethylene glycol(PEG)-ABS)through ligand exchange and amide condensation reaction.Ag_(2)S@PEG-ABS can selectively target tumors by surface-modified ABS and achieve accurate tumor imaging by the near infrared-II(NIR-II)fluorescence characteristics of Ag_(2)S QDs.PEG modification of Ag_(2)S QDs greatly improves its water solubility and stability,and therefore achieves high photothermal stability and high photothermal conversion efficiency(PCE)of 45.17%.Under laser irradiation,Ag_(2)S@PEG-ABS has powerful photothermal and inherent antitumor combinations on colon cancer cells(CT-26)in vitro.It also has been proved that Ag_(2)S@PEG-ABS can realize the effective treatment of hypoxia tumors in vivo and show good biocompatibility.Therefore,it is a new efficient integrated platform for the diagnosis and treatment of hypoxia tumors.

NIR-II荧光探针在癌症成像和术中导航的进展研究

分子成像技术使我们能够在分子水平上观察活组织的生理或病理过程,从而在早期准确诊断疾病。与近红外一区窗口(NIR-I,700~900nm)成像技术相比,近红外二区窗口(NIR-II,1000~1700nm)光学成像技术具有低自荧光、高信噪比、高组织穿透深度和较大的斯托克斯位移的优势。本文就NIR-II荧光探针的种类、肿瘤成像、血管成像及术中导航等外科方面的应用进展进行概述,旨在为该领域的进一步发展提供参考,争取早日实现临床转化。

Preparation of NIR-II-excited Drug-loaded Liposomes and Their Antitumor Activity and in Vitro Imaging Studies

In recent years,with the rapid development of nano-technology,the combination of diagnosis and treatment by nanotechnologyhas brought new hope for mankind to overcome cancer.The Near-infrared two-region(NIR-II)imaging technology hasdeveloped rapidly in recent years because of its non-invasive nature,strong tissue penetration and overall imaging of small animals.In this study,a novel nano-drug delivery system(DOX-IR1061 cationic liposomes)was prepared,loaded with doxorubicin(DOX)as a chemotherapeutic drug and NIR-II-excited fluorescent probe IR1061 as an imaging agent,and the uptake ability of tumor cellswas enhanced by octadecylamine.DOX-IR1061 cationic liposomes have good NIR-II imaging ability,clear imaging and obvioussignal.Cell uptake indicated that palamine could enhance the uptake efficiency of cationic liposomes by tumor cells,while in vitroanti-tumor experiments revealed that the enhancement of uptake efficiency would enhance the anti-tumor effect of DOX.Therefore,DOX-IR1061 cationic liposomes we prepared have the potential to realize both tumor imaging and therapy,and to realize theintegration of rapid diagnosis and treatment,and provide new ideas for cancer cure and the development of the field of diagnosis andtreatment integration.

可NIR-II荧光成像的多功能化疗栓塞微球PLGA@Re-DOX的制备及其在肝癌荧光手术导航中的应用

目的:开发具备近红外二区(Near-Infrared II,NIR-II)荧光成像性能的多功能化疗栓塞微球,评估其在肝癌NIR-II荧光手术导航中的应用效果。方法:制备NaGdF_(4):Nd@NaGdF_(4)纳米颗粒(Re NPs)和聚乳酸-羟基乙酸共聚物(Polylactic-co-glycolic Acid,PLGA)基微球,利用透射电子显微镜(Transmission Electron Microscope,TEM)和荧光光谱仪分析Re NPs形貌和荧光性能。PLGA基微球的形貌、元素组成通过光学显微镜、扫描电子显微镜(Scanning Electron Microscope,SEM)、X射线能谱分析(Energy Dispersive Spectroscopy,EDS)进行表征。利用紫外光谱分仪测定PLGA微球的阿霉素(Doxorubicin,DOX)装载和DOX释放情况。细胞增殖-毒性实验(Cell Counting Kit-8,CCK-8)和溶血实验来评估其生物相容性。利用NIR-II成像系统分析PLGA@Re-DOX微球的成像效果和荧光稳定性。通过肝动脉插管将PLGA@Re-DOX微球递送至大鼠肝癌部位,观察其对NIR-II荧光成像效果。结果:Re NPs展现出良好的单分散性和均一球形结构,平均粒径为9.3±0.4 nm,并具备优异的NIR-II荧光成像性能。PLGA、PLGA@Re-DOX微球尺寸分别为294±7μm、288±13μm,具有高度的均一性。PLGA微球具有良好的生物相容性,其对细胞存活率和溶血率与对照组相比没有显著统计差异(P>0.05)。此外,DOX包封率可达2.5%,能缓慢释放药物。PLGA@Re-DOX微球具有良好的肝内外NIR-II荧光成像效果和优异的荧光稳定性,经肝动脉递送后进行NIR-II荧光成像可以清晰地显示肿瘤边缘。结论:制备的PLGA@Re-DOX微球可有望实现对肝癌的NIR-II荧光成像和荧光手术导航。

In vivo real-time monitoring delayed administration of M2 macrophages to enhance healing of tendon by NIR-II fluorescence imaging

The administration time is a critical but long-neglected point in cell therapy based on macrophages because the incorrect time of macrophage administration could result in diverse outcomes regarding the same macrophage therapy.In this work,the second near-infrared(NIR-II)fluorescence imaging in vivo tracking of M2 macrophages during a pro-healing therapy in the mice model of rotator cuff injury revealed that the behavior of administrated macrophages was influenced by the timing of their administration.The delayed cell therapy(DCT)group had a longer retention time of injected M2 macrophages in the repairing tissue than that in the immediate cell therapy(ICT)group.Both Keller-Segel model and histological analysis further demonstrated that DCT altered the chemotaxis of M2 macrophages and improved the healing outcome of the repaired structure in comparison with ICT.Our results offer a possible explanation of previous conflicting results on reparative cell therapy and provoke reconsideration of the timing of these therapies.

Progress and Challenges of Water-soluble NIR-II Organic Fluorophores for Fluorescence Imaging In vivo

The small-molecule fluorophores for the second near-infrared(NIR-II,1000–1700 nm)window have attracted increasing attention in basic scientific research and preclinical practice owing to their deep-photo penetration,minimal physiological toxicity and simplicity of chemical modification.However,most of the reported small-molecule NIR-II fluorophores suffered from poor water solubility,which can easily cause organ toxicity.In addition,the aggregation caused by their poor water solubility in the aqueous solution would also result in weak fluorescence of these NIR-II fluorophores.Thus,it is highly desirable and valuable to develop water-soluble small-molecule NIR-II fluorophores with excellent photophysical properties for high-contrast in vivo imaging.In this review,we summarize the recent research advances in water-soluble small-molecule NIR-II fluorophores and highlight the representative bioimaging applications.Moreover,the potential challenges and perspectives of water-soluble small-molecule NIR-II fluorophores are discussed as well.We anticipate this review can help researchers to grab the latest information of water-soluble small-molecule fluorophores for NIR-II imaging,sequentially boosting their further development.

Self-shrinking supramolecular nanoparticles syndicate energy suppression and NIR-II mild photothermal amplification of mitochondrial oxidative stress for breast cancer therapy

Photothermal therapy(PTT)may lead to healthy tissue damage,tumor metastasis,and recurrence,which makes mild photothermal therapy(mild PTT)stand out.However,overcoming heat resistance,insufficient therapeutic effect,and poor photothermal conversion efficiency has become new challenge.Herein,we report a dynamic supramolecular nanocarrier formed from amide-sericin and aldehyde-polyhydroxy glucan(denoted as SDA),the loose cavity of which can be filled by using the pharmaceutical combination of lonidamine(LND)and NIR-II photothermal agent of IR-1061,producing SDLI with a tighter inner hole,smaller and uniform particle size and excellent stability due to multiple pulling forces.Moreover,the intricate internal network structure prevents the hydrophobic IR-1061 from forming aggregates in the small cavity,and the photothermal conversion efficiency(PCE)can reach 48.9%.At the acidic tumor microenvironment of pH 6.5,the controlled release of LND can solve the problem of heat resistance of NIR-II mild PTT and significantly improve the therapeutic effect of NIR-II mild PTT.Meanwhile,SDLI also shows a reasonable tumor inhibition rate,so the synergistic strategy of inhibiting tumor energy metabolism and NIR-II mild PTT to magnify mitochondrial oxidative stress,continuous cell stress state-induced immunogenic cell death to promote the induction of tumor apoptosis is proposed to achieve more effective cancer treatment.

NIR-II Organic Photothermal Cocrystals with Strong Charge Transfer Interaction for Flexible Wearable Heaters

The organic cocrystal strategy has provided a convenient and efficient platform for preparing organic photothermal materials.However,the rapidly directional preparation of cocrystals with desirable photothermal properties remains challenging due to a lack of suitable design ideas.Here,two new photothermal cocrystals,MTC and MFC,based on acceptor molecules(TCNQ and F4TCNQ)with different electron-withdrawing capacities were quickly prepared by the coprecipitation method,aiming to explore the effect of charge transfer(CT)interaction on photothermal properties.Compared with MTC,the stronger intermolecular CT interaction in MFC facilitates extending the absorption range(from the NIR-I to the NIR-II region)and enhancing the non-radiative transition process.Under the 808 nm laser irradiation,the photothermal conversion efficiency(PCE)of MFC is 54.6%,whereas MTC displays a mere 36.8%.The MFC cocrystal was further combined with a flexible polymer substrate(HPDMS)to prepare a flexible wearable heater(HPDMS@MFC),which exhibits excellent NIR-II photothermal performance.This work points out a research direction for the rapid assembly of efficient photothermal cocrystals and additionally provides an extensive application prospect for organic photothermal cocrystals in the field of wearable devices.

A rationally designed cancer vaccine based on NIR-II fluorescence image-guided light-triggered remote control of antigen cross-presentation and autophagy

Cancer vaccines represent a promising immunotherapeutic treatment modality.The promotion of cross-presentation of extracellular tumor-associated antigens on the major histocompatibility complex(MHC) class I molecules and dendritic cell maturation at the appropriate time and place is crucial for cancer vaccines to prime cytolytic T cell response with reduced side effects.Current vaccination strategies,however,are not able to achieve the spatiotemporal control of antigen cross-presentation.Here,we report a liposomal vaccine loading the second near-infrared window(NIR-II,1000—1700 nm) fluorophore BPBBT with an efficient photothermal conversion effect that offers an NIR-light-triggered endolysosomal escape under the imaging guidance.The NIR-II image-guided vaccination strategy specifically controls the cytosolic delivery of antigens for cross-presentation in the draining lymph nodes(DLNs).Moreover,the photothermally induced endolysosomal rupture initiates autophagy.We also find that the adjuvant simvastatin acts as an autophagy activator through inhibiting the PI3K/AKT/m TOR pathway.The light-induced autophagy in the DLNs together with simvastatin treatment cooperatively increase MHC class II expression by activating autophagy machinery for dendritic cell maturation.This study presents a paradigm of NIR-II image-guided light-triggered vaccination.The approach for remote control of antigen cross-presentation and autophagy represents a new strategy for vaccine development.

In vivo bioorthogonal labeling of rare-earth doped nanoparticles for improved NIR-II tumor imaging by extracellular vesiclemediated targeting

The development of efficient contrast agents for tumor-targeted imaging remains a critical challenge in the clinic.Herein,we proposed a tumor-derived extracellular vesicle(EV)-mediated targeting approach to improve in vivo tumor imaging using ternary downconversion nanoparticles(DCNPs)with strong near infrared II(NIR-II)luminescence at 1,525 nm.The EVs were metabolically engineered with azide group,followed by in vivo labeling of DCNPs through copper-free click chemistry.By taking advantage of the homologous targeting property of tumor derived EVs,remarkable improvement in the tumor accumulation(6.5%injection dose(ID)/g)was achieved in the subcutaneous colorectal cancer model when compared to that of individual DCNPs via passive targeting(1.1%ID/g).Importantly,such bioorthogonal labeling significantly increased NIR-II luminescence signals and prolonged the retention at tumor sites.Our work demonstrates the great potential of EVs-mediated bioorthogonal approach for in vivo labeling of NIR-II optical probes,which provides a robust tool for tumor-specific imaging and targeted therapy.

Enabling efficient NIR-II luminescence in lithium-sublattice core–shell nanocrystals towards Stark sublevel based nanothermometry

The luminescence in the second near-infrared(NIR-II)spectral region(1,000–1,700 nm)has recently attracted great attention for emerging biological applications owing to its merit of deep tissue bioimaging and high spatiotemporal resolution.However,it still remains a challenge to achieve the highly efficient NIR-II emissions of lanthanides in nanomaterials.Herein,we report an ideal design of sensitizing lithium sublattice core–shell nanocrystals for efficient NIR-II emission properties from a set of lanthanide emitters including Er3+,Tm3+,Ho3+,Pr3+,and Nd3+.In particular,the typical NIR-II emission of Er3+at 1.5μm was greatly enhanced by further manipulating the energy transfer via Er3+–Ce3+cross-relaxation,and the quantum yield can reach up to 35.74%under 980 nm excitation(12.5 W·cm−2),which is the highest value to the best of our knowledge.The 808 nm responsive efficient NIR-II emission was also enabled at the single-particle level through rational core–shell–shell structure design.Moreover,the lithium-sublattice provides an obvious spectral Stark-splitting feature,which can be used in the ultrasensitive NIR-II nanothermometer with relative sensitivity of 0.248%K−1 and excellent thermal cycling stability.These results open a door to the research of new kinds of efficient NIR-II luminescent materials,showing great promise in various frontier fields such as deep tissue nanothermometry and in vivo bioimaging.

Liquid exfoliation of stanene as degradable nanoagents for NIR-II photothermal therapy

Stanene,the two-dimensional form of elemental tin(Sn),is easily oxidized in the ambient environ-ment,significantly hindering its applications in biomedical fields.However,the degradation mechanism of stanene remains unclear.Herein,combined DFT calculations and proof-of-concept experiments were conducted to elucidate the underlying degradation mechanism of stanene.The results reveal that the degradation of stanene in an oxygenated water environment is a water-accelerated oxidation process.H_(2) O molecules could not only facilitate the electron transfer from stanene to O_(2) because of the polarization effect of H_(2) O,but also directly react with the defect sites of stanene due to enhanced absorption energy.Moreover,several protective strategies like alcohol protection were proposed to avoid or mitigate the oxidation of stanene for further applications.Finally,stanene was explored as the second near-infrared(NIR-II)photonic agents for ablation of 4T1 tumor,depicting a tumor-growth inhibition ratio up to 96.7%,much better than that of the first near-infrared(NIR-I)group(65.5%).This work reveals the degradation mechanism of stanene and demonstrates its biomedical applications in the NIR-II region.

NIR-II live imaging study on the degradation pattern of collagen in the mouse model

The degradation of collagen in different body parts is a critical point for designing collagen-based biomedical products.Here,three kinds of collagens labeled by second near-infrared(NIR-II)quantum dots(QDs),including collagen with low crosslinking degree(LC),middle crosslinking degree(MC)and high crosslinking degree(HC),were injected into the subcutaneous tissue,muscle and joints of the mouse model,respectively,in order to investigate the in vivo degradation pattern of collagen by NIR-II live imaging.The results of NIR-II imaging indicated that all tested collagens could be fully degraded after 35 days in the subcutaneous tissue,muscle and joints of the mouse model.However,the average degradation rate of subcutaneous tissue(k=0.13)and muscle(k=0.23)was slower than that of the joints(shoulder:k=0.42,knee:k=0.55).Specifically,the degradation rate of HC(k=0.13)was slower than LC(k=0.30)in muscle,while HC showed the fastest degradation rate in the shoulder and knee joints.In summary,NIR-II imaging could precisely identify the in vivo degradation rate of collagen.Moreover,the degradation rate of collagen was more closely related to the implanted body parts rather than the crosslinking degree of collagen,which was slower in the subcutaneous tissue and muscle compared to the joints in the mouse model.

Repurposing organic semiconducting nanomaterials to accelerate clinical translation of NIR-II fluorescence imaging

Optical imaging possesses important implications for early disease diagnosis,timely disease treatment,and basic medical as well as biological research.Compared with the traditionary near-infrared(NIR-I)window(650-950 nm)optical imaging,the emerging second near-infrared(NIR-II)window optical imaging technology owns the great superiorities of non-invasiveness,nonionizing radiation,and real-time dynamic imaging with the low biological interference,can significantly improve the tissue penetration depth and detection sensitivity,thus expecting to achieve accurate and precise diagnosis of major diseases.Inspired by the conspicuous superiorities,an increasing number of versatile NIR-II fluorophores have been legitimately designed and engineered for precisely deep-tissue mapping-mediated theranostics of life-threatening diseases.Organic semiconducting nanomaterials(OSNs)are derived from organic conjugated molecules withπ-electron delocalized skeletons,which show greatly preponderant prospects in the biomedicine field due to the excellent photoelectric property,tunable energy bands,and fine biocompatibility.In this review,the superiorities of NIR-II fluorescence imaging using OSNs for brilliant visualization various of diseases,including tongue cancer,ovarian cancer,osteosarcoma,bacteria or pathogens infection,kidney dysfunction,rheumatoid arthritis,liver injury,and cerebrovascular function,are emphatically summarized.Finally,the reasonable prospects and persistent efforts for repurposing OSNs to facilitate the clinical translation of NIR-II fluorescence phototheranostics are outlined.

NIR-II ?uorescence in vivo confocal microscopy with aggregation-induced emission dots

Significantly reduced tissue scattering of fluorescence signals in the second near-infrared(NIR-Ⅱ,1,000–1,700 nm)spectral region offers opportunities for large-depth in vivo bioimaging.Nowadays,most reported works concerning NIR-II fluorescence in vivo bioimaging are realized by wide-field illumination and 2D-arrayed detection(e.g.,via InGaAs camera),which has high temporal resolution but limited spatial resolution due to out-of-focus signals.Combining NIR-II fluorescence imaging with confocal microscopy is a good approach to achieve high-spatial resolution visualization of biosamples even at deep tissues.In this presented work,a NIR-II fluorescence confocal microscopic system was setup.By using a kind of aggregation-induced emission(AIE)dots as NIR-II fluorescent probes,800 lm-deep 3D in vivo cerebrovascular imaging of a mouse was obtained,and the spatial resolution at 700 lm depth could reach 8.78 lm.Moreover,the time-correlated single photon counting(TCSPC)technique and femtosecond laser excitation were introduced into NIR-II fluorescence confocal microscopy,and in vivo confocal NIR-II fluorescence lifetime microscopic imaging(FLIM)of mouse cerebral vasculature was successfully realized.

NIR-II light activated photodynamic therapy with proteincapped gold nanoclusters

The use of near-infrared （NIR） light for photodynamic therapy （PDT） is a promising strategy to circumvent the limitations of current PDT, in which visible light with limited tissue penetration depth is usually used. In the present stud~ alkyl thiolated gold nanoclusters （AuNCs） were co-modified with human serum albumin （HSA） and catalase （CAT）, and then employed as a multifunctional, optical, theranostic nano-agent. In the AuNC@HSA/CAT system, the AuNCs were able to produce singlet oxygen under excitation by a 1,064-nm laser, which locates in the second NIR window （NIR-II）, and featured much lower tissue absorption and scattering, enabling NIR-II-triggered PDT. The HSA coating greatly improved the physiological stability of the nanoparticles, which showed efficient tumor retention after intravenous injection, as revealed by detecting the AuNC fluorescence. Moreover, the presence of CAT in the nanoparticles triggered decomposition of tumor endogenous H202 to generate oxygen, thereby enhancing the efficacy of PDT by relieving tumor hypoxia. Compared with conventional PDT using visible light, NIR-II-triggered PDT exhibits remarkably increased tissue penetration. Thus, we developed a new type of photosensitizing nano-agent that simultaneously enables in vivo fluorescence imaging, tumor hypoxia relief, and NIR-II light-induced in vivo PDT in the treatment of cancer.

Zn-doping enhances the photoluminescence and stability of PbS quantum dots for in vivo high-resolution imaging in the NIR-II window

Lead sulfide(PbS)quantum dots(QDs)are important near infrared(NIR)luminescent materials with tunable and strong emission covering a broad NIR region.However,their optical properties are quite sensitive to air,water,and high temperature due to the surface oxidation,thus limiting their applications in optoelectronic devices and biological imaging.Herein,a cation-doping strategy is presented to make a series of high-quality Zn-doped PbS QDs with strong emission covering whole second near-infrared window(NIR-II,1,000-1,700 nm).First-principle calculations confirmed that Zn dopants formed dopant states and decreased the recombination energy gap of host PbS.Notably,the Zn dopants significantly improved the quantum yield,photoluminescence lifetime and thermal stability of PbS QDs.Moreover,the PEGylated Zn-doped PbS QDs emitting in the NIR-llb window(1,500-1,700 nm)realized the noninvasive imaging of cerebral vascular of mouse with high resolution,being able to distinguish blood capillary.This material not only provides a new tool for deep tissue fluorescence imaging,but is also promising for the development of other NIR related devices.

Tracking the in vivo spatio-temporal patterns of neovascularization via NIR-II fluorescence imaging

The in vivo spatio-temporal patterns of neovascularization are still poorly understood because it is limited to multi-scale techniques from the cellular level to living animal level.Owing to deep tissue-penetration and zero autofluorescence background,the second near-infrared(NIR-II,1,000–1,700 nm)fluorescence imaging recently shows promise in breaking through this dilemma by dynamically tracking the pathophysiological process of neovascularization in vivo.Here,NIR-II fluorescence imaging was recruited for monitoring blood vessels in order to visualize the vascular injury and quantitively assess neovascularization in mouse models of acute skeleton muscle contusion and hindlimb ischemia.The temporal analysis of real-time NIR-II fluorescence intensity demonstrated that the blood flow perfusion of ischemia area was able to rapidly restore to 96%of pre-ischemic state within one week.Moreover,the spatial analysis revealed that the lower and outer quadrants of ischemia area in the mouse model of hindlimb ischemia always had relatively high blood flow perfusion compared with other quadrants during three weeks post-ischemia,and even exceeded pre-ischemic quantity at 21 days post-ischemia.In conclusion,this in vivo imaging technique has significant potential utility for studying the spatio-temporal patterns of neovascularization in vivo.

In vivo and in situ real-time fluorescence imaging of peripheral nerves in the NIR-II window

The peripheral nervous system(PNS)is essential for performing and maintaining various motor and sensory functions.Abnormalities can lead to a series of peripheral neurological conditions,such as paraesthesia,pain,or spasms,which are debilitating and lowering the quality of life.Thecurrent guidelines for diagnosis rely predominantly on clinical symptoms resulting from PNS dysfunction,which occur already at an advancedstage.There are currently no effective methods that visually reflect the extent of peripheral neuropathy.In our study,we present a novel in vivoand in situ real-time imaging of peripheral nerves based on the second near-infrared window(NIR-II)fluoresce nee.In NIR-II system,lead sulfidequa ntum dots(PbS Qds)with NIR-II fluoresce nee specifically bound to motor neuro rvspecific protein agrin,acting as image con trast.In micemodel,peripheral nerves were visible as soon as after 2 h post injection.We provide evidenee for the efficacy of this approach,which allows todirectly dem on strate peripheral nerves,their structure,and pote ntial damagesites and degree.Furthermore,our products were of goodbiocompatibility,while the n eural fluoresce nee signal was solid,bright and stable for 4 h in vivo.Thus,overall,our results suggest that NIR-II isan effective new method for direct imaging of peripheral nerves in vivo,opening new horizons on early,improved and more precise,targeteddiag no sis.A resulti ng more rapid installatio n of perso nalized therapy facilitates a better prognosis of clinical peripheral neuropathy.

NIR-II emissive dye based polymer nanoparticle targeting EGFR for oral cancer theranostics

Oral cancer is a common malignant tumor of the head and neck,and surgery combined with radiotherapy and chemotherapy is the primary treatment modality.However,a positive resection margin that may lead to recurrence after surgery has always been a critical issue to address.Furthermore,radiotherapy and chemotherapy also have shortcomings such as resistance to chemotherapy and radiation,lack of targeting,and severe side effects.Therefore,exploring new methods of tumor surgical navigation and tumor treatment is of great significance for oral cancer.Although,the emerging near-infrared II(NIR-II,1,000–1,700 nm)region fluorescent imaging has revolutionized surgical navigation,a high tumor-targeting fluorescent probe remains lacking.Furthermore,while emerging photothermal therapy(PTT)can overcome chemoradiotherapy’s shortcomings and achieve precise treatment of tumors,its clinical application is still limited by the lack of high photothermal conversion efficiency,high photothermal stability,and highly penetrating materials.Herein,a NIR-II dye SQ890 is developed for tumor imaging and PTT of oral cancer.By assembling into nanoparticles(NPs)and being modified with epithelial growth factor receptor(EGFR)-targeting peptides GE11,SQ890 NPs-Pep can specifically accumulate in tumor sites via active targeting,and realize photoacoustic/NIR-II fluorescence dual-modality imaging-guided PTT of oral cancer.