Supercritical carbon dioxide （SC-CO2） extraction was employed to extract oil from Nigella glandulifera Freyn seed in this study. Response surface methodology （RSM） was applied to evaluate the effects of the proces...Supercritical carbon dioxide （SC-CO2） extraction was employed to extract oil from Nigella glandulifera Freyn seed in this study. Response surface methodology （RSM） was applied to evaluate the effects of the process parameters （pressure, temperature, and CO2 flow rate） on oil yield of N. glandulifera seed. A Box-Behnken design was used to optimize the extraction parameters. The analysis of variance indicated that the linear coefficients of pressure and CO2 flow rate, the quadratic term coefficients of pressure and temperature and the interactions between pressure and temperature, as well as temperature and CO2 flow rate, had significant effects on the oil yield （P〈0.05）. The optimal conditions to obtain the maximum oil yield from N. glandulifera seed were pressure 30.84 MPa, temperature 40.57°C, and CO2 flow rate 22.00 L h-1. Under these optimal conditions, the yield of oil was predicted to be 38.19%. The validation experiment results agreed with the predicted values. The fatty acid composition of N. glandulifera seed oil extracted using SC-CO2 was compared with that of oil obtained by Soxhlet method. The results showed that the fatty acid compositions of oil extracted by the two methods were similar. Identification of oil compounds with gas chromatography-mass spectrometry （GC-MS） showed that the contents of unsaturated fatty acids linoleic acid （48.30%）, oleic acid （22.28%） and saturated fatty acids palmitic acid （16.65%）, stearic acid （4.17%） were the most abundant fatty acids in seed oil from N. glandulifera.展开更多
目的:优选黑种草子总皂苷滴丸的制备工艺。方法:以滴丸的外观质量评分、丸重量差异变异系数和溶散时限的综合评分作为考核指标,通过单因素和正交试验对影响滴丸成型的因素进行考察,优选最佳制备工艺。结果:优选的最佳制备工艺为:以PEG4...目的:优选黑种草子总皂苷滴丸的制备工艺。方法:以滴丸的外观质量评分、丸重量差异变异系数和溶散时限的综合评分作为考核指标,通过单因素和正交试验对影响滴丸成型的因素进行考察,优选最佳制备工艺。结果:优选的最佳制备工艺为:以PEG4000∶PEG6000(5∶1)为基质,总皂苷提取物与基质配比1∶3,滴制温度80℃,冷却剂为二甲基硅油(运动黏度350 m Pa·s),冷却温度5℃,滴距10 cm,滴制速度30滴/min。结论:优选出的制备工艺稳定可行。展开更多
基金supported by the Public Welfare Industry (Agriculture) Research Program,China (200903018)
文摘Supercritical carbon dioxide （SC-CO2） extraction was employed to extract oil from Nigella glandulifera Freyn seed in this study. Response surface methodology （RSM） was applied to evaluate the effects of the process parameters （pressure, temperature, and CO2 flow rate） on oil yield of N. glandulifera seed. A Box-Behnken design was used to optimize the extraction parameters. The analysis of variance indicated that the linear coefficients of pressure and CO2 flow rate, the quadratic term coefficients of pressure and temperature and the interactions between pressure and temperature, as well as temperature and CO2 flow rate, had significant effects on the oil yield （P〈0.05）. The optimal conditions to obtain the maximum oil yield from N. glandulifera seed were pressure 30.84 MPa, temperature 40.57°C, and CO2 flow rate 22.00 L h-1. Under these optimal conditions, the yield of oil was predicted to be 38.19%. The validation experiment results agreed with the predicted values. The fatty acid composition of N. glandulifera seed oil extracted using SC-CO2 was compared with that of oil obtained by Soxhlet method. The results showed that the fatty acid compositions of oil extracted by the two methods were similar. Identification of oil compounds with gas chromatography-mass spectrometry （GC-MS） showed that the contents of unsaturated fatty acids linoleic acid （48.30%）, oleic acid （22.28%） and saturated fatty acids palmitic acid （16.65%）, stearic acid （4.17%） were the most abundant fatty acids in seed oil from N. glandulifera.
文摘目的:优选黑种草子总皂苷滴丸的制备工艺。方法:以滴丸的外观质量评分、丸重量差异变异系数和溶散时限的综合评分作为考核指标,通过单因素和正交试验对影响滴丸成型的因素进行考察,优选最佳制备工艺。结果:优选的最佳制备工艺为:以PEG4000∶PEG6000(5∶1)为基质,总皂苷提取物与基质配比1∶3,滴制温度80℃,冷却剂为二甲基硅油(运动黏度350 m Pa·s),冷却温度5℃,滴距10 cm,滴制速度30滴/min。结论:优选出的制备工艺稳定可行。
