Exploring the therapeutic potential of Qi Teng Mai Ning recipe in ischemic stroke and vascular cognitive impairment

Background:This study aims to explore the therapeutic effects of the Qi Teng Mai Ning recipe on ischemic stroke and vascular cognitive impairment through its potential to modulate cellular autophagy,with a focus on identifying its active ingredients and their target proteins.Methods:The study began with the identification of active ingredients in the Qi Teng Mai Ning recipe.It proceeded to screen the gene expression omnibus database for ischemic stroke and vascular cognitive impairment-associated differentially expressed mRNAs and to identify cellular autophagy-related proteins via the Human Autophagy Database.These proteins were annotated with Gene Ontology and Kyoto Encyclopedia of Genes and Genomes functions and subjected to molecular docking with the recipe’s core active ingredients.In vitro cell experiments were conducted on hypoxic HT22 cells,involving CCK8 assay,lentiviral transfection to silence autophagy related 9B(ATG9B),immunofluorescence staining,and qPCR validation to investigate the effects of the recipe on autophagy.Results:The analysis identified 104 active ingredients targeting 408 proteins and forming a complex ingredient-target network.Intersecting 55 ischemic stroke-related and 909 vascular cognitive impairment-related differentially expressed mRNAs revealed 14 co-expressed mRNAs.Molecular docking showed quercetin,kaempferol,myrcene,and conferone as key ingredients targeting autophagy-related proteins.Cellular experiments indicated that the recipe significantly enhanced cell viability under hypoxic conditions,reduced apoptosis,and modulated the expression of autophagy-related factors,thereby decreasing apoptosis rates in HT22 cells.Conclusion:The Qi Teng Mai Ning recipe offers protective effects against ischemic stroke and vascular cognitive impairment by modulating autophagy-related proteins.Its efficacy highlights the potential of traditional Chinese medicine in treating these conditions,though further research is needed to fully understand its mechanisms and clinical applications.

SOME ALGORITHMS FOR NC MACHINING SIMULATION AND VERIFICATION

This paper presents an approximation method to display realistic pictures of numerical control (NC) machining simulation very quickly. T he tool movement envelope is divided into many small regions and the normal to t hese small regions is calculated. The system saves the calculated result in a fi le before starting animation display. When the system starts displaying machinin g animation, it does not need to calculate small triangular facets normal to the workpiece surface. It only needs to find out what part of the cutter cuts the w orkpiece surface and to read the normal from the file. A highly efficient NC cod e verification method is also presented in this paper. The method first detects the error in z direction. If some points are reported to be out of the tolerance , the system divides neighborhood of these points into smaller grids and calcula tes the normal surface at each grid intersection and the error in the normal ve ctor direction.

Influence of confi ning pressure and impact loading on mechanical properties of amphibolite and sericite-quartz schist

In order to investigate the dynamic mechanical properties of amphibolite and sericite-quartz schist under confi ning pressure, two rocks are subjected to impact loadings with different strain rates and confi ning pressures by using split Hopkinson pressure bar equipment with a confi ning pressure device. Based on the experimental results, the stress-strain curves are analyzed and the effects of confi ning pressure and strain rates on the dynamic compressive strength, peak strain and failure mode are summarized. The results show that:(1) The characteristics of two rocks in the ascent stage of the stressstrain curve are basically the same, but in the descent stage, the rocks gradually show plastic deformation characteristics as the confi ning pressure increases.(2) The dynamic compressive strength and peak strain of two rocks increase as the strain rate increases and the confi ning pressure effects are obvious.(3) Due to the effect of confi ning pressure, the normal stress on the damage surface of the rock increases correspondingly, the bearing capacity of the crack friction exceeds the material cohesion and the slippage of the fractured rock is controlled, which all lead to the compression and shear failure mode of rock. The theoretical analysis and experimental methods to study the dynamic failure mode and other related characteristics of rock are useful in developing standards for engineering practice.

Water Extraction Process of Chinese Herbal Compound Man Gan Ning

[Objectives]To optimize the water extraction process of Chinese Herbal Compound Man Gan Ning and establish a method for its extraction and content determination.[Methods]The L9(34)orthogonal test was carried out,the comprehensive scores of ginsenoside Rb1 content,notoginsenoside R1 content,tanshinone I content and saikosaponin A content in the compound extract were used as evaluation indicators to explore the effects of water addition,the water addition,decoction time and decoction times on the water extraction process,and verification tests were carried out.[Results]The optimized water extraction process was as follows:adding 9 times of water in the first extraction,and extracting for 90 min;adding 7 times of water in the second extraction,and extracting for 60 min;adding 7 times of water in the third extraction,and extracting for 60 min.[Conclusions]The optimized water extraction process is stable and feasible,and can be used for the extraction of various herbs in Compound Man Gan Ning.It can also provide experimental basis for the formulation development of Compound Man Gan Ning sustained release tablet.

The Lost-Wax Process in the Pre-Qin Period: A Bronze Appendage with Hollow Snake Patterns from the Tomb of Duke Ning of Xu

This paper discusses the most recent case of the lost-wax process,namely,a bronze appendage with hollow snake patterns excavated in 2002 at the tomb of Duke Ning of Xu(許公寧)(a state in the Spring and Autumn period)in Ye County,Henan Province.Various physical and chemical analyses indicate that this artifact was cast as a whole in 547 BCE or slightly later in a high tin bronze alloy using the lost-wax process.No traces of cast joints or soldering were found.This research provides further proof that the lost-wax process was a significant technical development achieved by casters in the Pre-Qin period.

Jian Nao Ning for Treatment of Memory Impairment in Patients with Mild or Moderate Multi-infarct Dementia

Forty patients with multi-infarct dementia (MID) were randomly assigned to the treatment group (25 cases) treated with Jian Nao Ning (健脑宁JNN) and the duxil control group (15 cases). Memory function were assessed at baseline and endpoint using memory subscales of a battery of New Psychometric Tests (Chinese version) including mini-mental state examination (MMSE), verbal memory, and non-verbal memory, etc. After treatment, the mean scores of verbal memory in the Hopkins Verbal Learning Test (P<0.05) and total memory scores of memory items (P<0.001) in JNN group increased significantly; and improvement in episodic memory function including story recall (immediate and delayed), delayed word recall, verbal learning and verbal recognition and visual recognition in the JNN group was better than that in the duxil control group, suggesting that JNN can obviously improve memory function for the patients with mild or moderate multi-infarct dementia.

Research on the Accurate Location of the 2007 M_S6.4 Ning'er, Yunnan Earthquake

Five mobile digital seismic stations were set up by the Earthquake Administration of Yunnan Province near the epicenter of the main shock after the Ning＇er M6. 4 earthquake on June 3, 2007. In this paper, the aftershock sequence of the Ning＇er M6. 4 earthquake is relocated by using the double difference earthquake location method. The data is from the 5 mobile digital seismic stations and the permanent Simao seismic station. The results show that the length of the aftershock sequence is 40kin and the width is 30km, concentrated obviously at the lateral displacement area between the Pu＇er fault and the NNE-trending faults, with the majority occurring on the Pu＇er fault around the main shock. The depths of aftershocks are from 2kin to 12km, and the predominant distribution is in the depth of 8 ~ 10km. The mean depth is 7. 9kin. The seismic fault dips to the northwest revealed from the profile parallel to this aftershock sequence, which is identical to the dip of the secondary fault of the NE-trending Menglian-Mojiang fault in the earthquake area. There are more earthquakes concentrated in the northwest segment than in the southeast segment, which is perhaps related to the underground medium and faults. The depth profile of the earthquake sequence shows that the relocated earthquakes are mainly located near the Pu＇er fault and the seismic faults dip to the southwest, consistent with the dip of the west branch of the Pu＇er fault. In all, the fault strike revealed by earthquake relocations matches well with the strike in the focal mechanism solutions. The main shock is in the top of the aftershock sequence and the aftershocks are symmetrically distributed, showing that faulting was complete in both the NE and SW directions.

Ning Yuhua:An Expert on Wheat Breeding

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Integration of single-cell RNA sequencing and network disturbance to elucidate crosstalk between multi-components drug and trigeminal ganglia cells in migraine

OBJECTIVE To clarify the mecha⁃nism of Tou Tong Ning capsule(TTNC)and elu⁃cidate crosstalk between multi-components drug and trigeminal ganglia cells in migraine.METH⁃ODS We integrated single-cell RNA sequencing data and a quantitative evaluation algorithm of the disturbance of multi-target drugs on the dis⁃ease network to explore the specific pathology of TTNC for migraine.Cerebrovascular smooth muscle spasmolytic activity experiment was car⁃ried out to verify the results of bioinformatics analysis.RESULTS TTNC can effectively regu⁃late the functions of synaptic signaling,inflamma⁃tion and immune response,and cerebrovascular smooth muscle.For different cell subtypes of tri⁃geminal ganglia,TTNC can significantly disturb the robustness on neuronal cell networks and non-neural networks(fibroblast and vascular cells),indicating TTNC vasodilation activity of brain vessels and neural regulation activities of various neuro types.Contraction of cerebrovas⁃cular smooth muscle of mouse ex vivo confirmed the vasodilation activity of TTNC and active com⁃pounds(Emodin,Luteolin and Levistilide A).And literature mining confirmed the vasospasmolytic activity and neuroprotective effect activity of TTNC.CONCLUSIONS Integrating single-cell data and network disturbance tools provides a new strategy for the MoA of multi-components drug through cell subtyping.

华荠苧响应不同土壤水分的表型可塑性

研究了土壤相对含水量分别为 1 0 0 %、80 %、60 %、40 %和 30 %水分条件下华荠 ( Mosla chinensis)生长和形态的表型可塑性。结果表明 :成年植株的生物量积累、形态参数、单株叶绿素总含量及穗重等都在60 %相对含水量条件下最高 ;当土壤相对含水量从 30 %提高到 60 %时 ,分配到枝和根的生物量随之增加 ,分配到叶的生物量减少 ,土壤相对含水量再提高时 ,情况则相反 ;生殖比率随土壤相对含水量的下降而升高 ,30 %处理最大 ,1 0 0 %处理最小。这些结果说明华荠的适宜水分生态位是中偏湿的土壤环境。华荠通过器官生物量分配和形态结构调整对不同土壤水分产生可塑性响应 ,并以提高生殖比率的策略来适应干旱胁迫。

杭州石荠苧(Mosla hangchowensis)蒸腾的生理生态学研究

根据连续３年的野外种群生态和生理生态学研究中发现的问题，研究了控制条件下杭州石荠蒸腾的生理生态特征。结果表明：１）杭州石荠的蒸腾日进程曲线峰高随土壤含水量（θ）的降低而降低；２）蒸腾速率（Ｔｒ）与空气相对湿度在饱和与中湿土壤上为负相关，而在干旱土壤上为正相关，体现了该种在土壤干旱时的自我保护机制；３）薄云、多水汽和中午多云的晴天，日蒸腾量均较低，分别为２．６和２．８ｍｏｌｇ－１ｄ－１，典型晴天的日蒸腾量较高，为３．４ｍｏｌｇ－１ｄ－１；４）在遮荫（４０％全光照）条件下，Ｔｒ平均降低７６％，且Ｔｒ对气温、湿度的响应程度小于全光照条件，对光强的响应则大于全光照条件；５）当气温为１５～１８℃时，在诸环境因子中Ｔｒ只与θ有关。该种的水分生理生态特性十分特别，具有从中生到旱生植物的某些生理生态特性。上述结果解释了杭州石荠种群占据现有生态位——向阳开阔地薄土生境的生理生态机制，分析了其不能向厚土生境扩展分布地的原因。

杭州石荠苧(唇形科)小坚果的散布生物学研究

报道了杭州石荠（ＭｏｓｌａｈａｎｇｃｈｏｕｅｎｓｉｓＭａｔｓｕｄａ）的种子散布机制和特点，结合其它方面的研究结果，为迁地保护提供理论依据。杭州石荠小坚果的散布机制是被动弹力散布，植株、花萼和小坚果三者联合参与，风、雨、动物触动是能量来源。初级散布距离在实验条件下，在３０～３５ｃｍ的区域小坚果最多，此后，随面积的增加而减少，密度峰值出现在１０～１５ｃｍ的区间。杭州石荠的小坚果表面具旋涡状凹陷，凹陷中有细密的突起，这种结构有一定的拒水性，下沉速率小，适于以水为载体的次级散布，也具有较强的保水抗旱能力，能够确保杭州石荠在干旱的生境中世代延续。作者认为，杭州石荠的分布特性和比较特殊的生物学和生态学特点限制了居群的扩展是其濒危的重要原因。

濒危植物杭州石荠物质积累和分配对光的响应

以1年生濒危植物杭州石荠为试验材料,人工控制光照条件,设计了全光照、80%、60%、40%、20%和10%自然光照6个光强梯度(分别以L100～L10表示)处理,研究了不同处理下杭州石荠各器官的物质积累与分配、空间构件指标和生殖动态随光照强度的变化.结果表明:杭州石荠苗期对光强的变化不敏感,营养生长期末和生殖期末差异显著(P<0.05).弱光对杭州石荠的生长不利,低光强下杭州石荠在苗期大量死亡;全光照下杭州石荠的生物量积累不及L80;杭州石荠生物量积累的最适光梯度是L80,但在此光强下的生殖比率不及L60和L100处理.

华荠薴中的黄酮类化合物

从华荠（ＭｏｓｌａｃｈｉｎｅｎｓｉｓＭａｘｉｍ）中分离鉴定了６种黄酮类化合物，经光谱分析及化学方法鉴定为：５－羟基－６，７－甲基黄酮；５，７－二羟基－４＇－甲氧基黄酮；洋芹素；鼠李柠檬素；桑色素－７－Ｏ－β－Ｄ－葡萄糖甙；山奈素－３－Ｏ－β－Ｄ－葡萄糖甙．均为首次从该植物中分得．

生血宁治疗缺铁性贫血的临床研究

蚕砂中提取叶绿素、经皂化、铁代精制成铁叶绿酸钠,并以此为原料制成生血宁片,治疗缺铁性贫血180例,以硫酸亚铁片作对照。结果成人治疗组120例,治愈108例占90％,显效12例占10％;成人对照组30例治愈10例,占33.33％,显效8例占26.67％,有效6例占20％,无效6例占20％。儿童治疗组60例,治愈52例占86.67％,显效8例占13.33％。儿童对照组30例,治愈13例占43.33％,显效5例占16.67％,有效4例占13.33％,无效8例占26.67％,生血宁片疗效明显优于硫酸亚铁片,且无明显毒副反应,易为广大病人接受值得推广应用。

四川优质苧麻的结构和性能研究

本文采用扫描电镜、Ｘ射线衍射仪以及常规仪器分析的方法测试了四川优质麻－达县红皮小麻和大竹青白麻的形态结构，超分子结构和力学性能，并与棉纤维进行了对比．实验表明，精干麻表面光洁．有裂纹裂缝等结构不匀点，取向度高，结晶度高，断裂强度高，刚性和断裂比功大，而延伸小；纤维粗．在主要指标上，红皮小麻优于青白麻。

小鱼仙草和石荠苧的鉴别研究

对小鱼仙草和石荠艹宁 的茎叶组织结构特征、种子、花粉的形态特征进行对比观察研究 .结果表明 :其种子纹饰、叶表皮特征、茎叶组织结构特征等是两种植物鉴别的重要依据 .对相同产地同一生境条件下两种植物全草挥发油的化学成分进行对比分析研究 ,两种植物挥发油均含有的相同成分有 :香荆芥酚、百里香酚、β-石竹烯、檀香烯、艹律 草烯、甲基丁香油酚、异胡薄荷酮等 2 3种成分 .相同的成分分别占小鱼仙草和石荠艹宁 总精油的 83.2 6 %和 72 .78% .近 1 8%的化学成分是不同的 ,即使是相同的成分 ,其含量也存在比较明显差异 .