Accurate species identification is a key component of biodiversity research.DNA barcoding is an effective molecular method used for fish species identification.We aimed to study the DNA barcoding of fish in Zhoushan c...Accurate species identification is a key component of biodiversity research.DNA barcoding is an effective molecular method used for fish species identification.We aimed to study the DNA barcoding of fish in Zhoushan coastal waters,explore the differences and applicability of two gene fragments(12S rRNA and COI)of DNA barcoding in fish species identification,and established a comprehensive fish barcoding reference database.Two hundred and eighty-seven captured fish samples from Zhoushan coastal waters were identified using morphological characteristics and DNA barcoding.A total of 26412S rRNA sequences(belonging to eight orders,31 families,55 genera,and 66 species)and 188 COI sequences(belonging to seven orders,30 families,48 genera,and 58 species)were obtained.The lengths of the 12S rRNA sequences ranged from 165 to 178 bp,and the guanine-cytosine(GC)content was 45.37%.The average 12S rRNA interspecific and intraspecific genetic distances(K2P)were 0.10%and 26.66%,respectively.The length of the COI sequence ranged 574–655 bp,and the content of GC was 45.97%.The average 12S rRNA interspecific and intraspecific genetic distances(K2P)were 0.16%and 27.45%,respectively.The minimum interspecific genetic distances of 12S rRNA and COI(1.23%and 1.86%)were both greater than their maximum intraspecific genetic distances(2.42%and 8.66%).Three molecular analyses(NJ tree,ABGD,and GMYC)were performed to accurately identify and delineate species.Clustering errors occurred when the 12S rRNA sequences were delimited using the NJ tree method,and the delimitation results of ABGD and GMYC are consistent with the final species identification results.Our results demonstrate that DNA barcoding based on 12S rRNA and COI can be used as an effective tool for fish species identification,and 12S rRNA has good application prospects in the environmental DNA(eDNA)metabarcoding of marine fish.展开更多
Letting the history and culture of bread in France tell the story of its people in China.“Ningbo is a bustling city thanks to its very enterprising residents and the pleasant living environment,”said Samy Snoussi,ow...Letting the history and culture of bread in France tell the story of its people in China.“Ningbo is a bustling city thanks to its very enterprising residents and the pleasant living environment,”said Samy Snoussi,owner of the French bakery Monépoque in Ningbo,a coastal city in eastern China’s Zhejiang Province.Snoussi moved to Ningbo in 2006.To overcome his homesickness and promote authentic French bread in the city,he opened a bakery,which soon became popular and earned him the nickname“French uncle”among local bakers.展开更多
随着我国港口吞吐量日益增长,沿海水域交通变得繁忙且船舶事故时有发生。以舟山水域作为研究对象,利用热力图、DBSCAN聚类算法及Getis-Ord General G分析方法对舟山船舶事故数据进行综合分析,识别水域交通事故黑点,提供事故的空间分布,...随着我国港口吞吐量日益增长,沿海水域交通变得繁忙且船舶事故时有发生。以舟山水域作为研究对象,利用热力图、DBSCAN聚类算法及Getis-Ord General G分析方法对舟山船舶事故数据进行综合分析,识别水域交通事故黑点,提供事故的空间分布,并结合经纬度和事故严重程度信息揭示了潜在的高风险区域,从而为地区海事安全管理与事故预防提供科学依据。展开更多
基金Supported by the Zhejiang Provincial Key Research and Development Program (No.2021C02047)。
文摘Accurate species identification is a key component of biodiversity research.DNA barcoding is an effective molecular method used for fish species identification.We aimed to study the DNA barcoding of fish in Zhoushan coastal waters,explore the differences and applicability of two gene fragments(12S rRNA and COI)of DNA barcoding in fish species identification,and established a comprehensive fish barcoding reference database.Two hundred and eighty-seven captured fish samples from Zhoushan coastal waters were identified using morphological characteristics and DNA barcoding.A total of 26412S rRNA sequences(belonging to eight orders,31 families,55 genera,and 66 species)and 188 COI sequences(belonging to seven orders,30 families,48 genera,and 58 species)were obtained.The lengths of the 12S rRNA sequences ranged from 165 to 178 bp,and the guanine-cytosine(GC)content was 45.37%.The average 12S rRNA interspecific and intraspecific genetic distances(K2P)were 0.10%and 26.66%,respectively.The length of the COI sequence ranged 574–655 bp,and the content of GC was 45.97%.The average 12S rRNA interspecific and intraspecific genetic distances(K2P)were 0.16%and 27.45%,respectively.The minimum interspecific genetic distances of 12S rRNA and COI(1.23%and 1.86%)were both greater than their maximum intraspecific genetic distances(2.42%and 8.66%).Three molecular analyses(NJ tree,ABGD,and GMYC)were performed to accurately identify and delineate species.Clustering errors occurred when the 12S rRNA sequences were delimited using the NJ tree method,and the delimitation results of ABGD and GMYC are consistent with the final species identification results.Our results demonstrate that DNA barcoding based on 12S rRNA and COI can be used as an effective tool for fish species identification,and 12S rRNA has good application prospects in the environmental DNA(eDNA)metabarcoding of marine fish.
文摘Letting the history and culture of bread in France tell the story of its people in China.“Ningbo is a bustling city thanks to its very enterprising residents and the pleasant living environment,”said Samy Snoussi,owner of the French bakery Monépoque in Ningbo,a coastal city in eastern China’s Zhejiang Province.Snoussi moved to Ningbo in 2006.To overcome his homesickness and promote authentic French bread in the city,he opened a bakery,which soon became popular and earned him the nickname“French uncle”among local bakers.
文摘随着我国港口吞吐量日益增长,沿海水域交通变得繁忙且船舶事故时有发生。以舟山水域作为研究对象,利用热力图、DBSCAN聚类算法及Getis-Ord General G分析方法对舟山船舶事故数据进行综合分析,识别水域交通事故黑点,提供事故的空间分布,并结合经纬度和事故严重程度信息揭示了潜在的高风险区域,从而为地区海事安全管理与事故预防提供科学依据。
