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Effects of Exogenous H_(2)O_(2) on ROS Metabolism in Nitraria tangutorum Bobr. Leaves Under Salt Stress
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作者 Zhang Nan Cahaeraduqin Sunaer +3 位作者 Du Jinxue Pan Yiliang Wang Rui Liu Wei 《Journal of Northeast Agricultural University(English Edition)》 CAS 2024年第1期20-27,共8页
Salt stress is a major abiotic stress limiting plant growth and yield. In the present study, the effects of exogenous H_(2)O_(2) on the reactive oxygen species(ROS) metabolism and the antioxidant system in leaves of N... Salt stress is a major abiotic stress limiting plant growth and yield. In the present study, the effects of exogenous H_(2)O_(2) on the reactive oxygen species(ROS) metabolism and the antioxidant system in leaves of Nitralia tangutorum Bobr. under salt stress were studied. N. tangutorum seedlings were subjected to 200 mmol·L^(-1) NaCl treatment with or without the exogenous application of H_(2)O_(2) for 7 days. The results showed that NaCl stress significantly increased the relative conductivity, the contents of thiobarbituric acid reactive substances(TBARS) and ROS(H_(2)O_(2) and O_(2)^(·-)), as well as promoted the activities of antioxidant enzymes including superoxide dismutase(SOD), peroxidase(POD), catalase(CAT), and ascorbate peroxidase(APX) in N. tangutorum leaves. In addition, exogenous H_(2)O_(2) decreased the relative conductivity, the contents of TBARS, H_(2)O_(2) and O_(2)^(·-), while further enhanced the activities of antioxidant enzymes. These results indicated that H_(2)O_(2) effectively alleviated the adverse effects of NaCl stress on N. tangutorum through the regulation of ROS metabolism. 展开更多
关键词 H_(2)O_(2) NaCl stress reactive oxygen species antioxidant enzymes nitraria tangutorum Bobr
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On Spatial Variability and Distribution of Soil Nutrients in Different Nitraria tangutorum Bobr.Populations in Tsaidam Basin 被引量:1
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作者 苏艳 邱权 +5 位作者 何茜 李吉跃 王军辉 王宁 孙奎 贾子瑞 《Agricultural Science & Technology》 CAS 2015年第2期285-291,共7页
In this paper,six important distribution areas of Nitraria tangutorum Bobr.in Tsaidam Basin were selected as research objects,to study spatial variability and distribution of soil nutrients in different N.tangutorum p... In this paper,six important distribution areas of Nitraria tangutorum Bobr.in Tsaidam Basin were selected as research objects,to study spatial variability and distribution of soil nutrients in different N.tangutorum populations and analyze the relationship between soil nutrient contents and geographical location,by measuring soil pH and the contents of organic matter(OM),total nitrogen(N),total phosphorus(P),total potassium(K),hydrolysis N,available P and available K in soils.Results showed that:(1) soil nutrient contents among different populations showed significant spatial variability,and soil depth had a significant effect on soil nutrients contents,but the variation rules were not obvious.(2)Average pH and average contents of OM,total N,total P,total K,hydrolysis N,available P and available K in soils with different depths(0-15,15-30,30-45 cm) varied in the range of 8.37-9.21,3.34-20.68,0.18-1.21,0.35-0.75,16.12-22.04,5.13-553.28,1.10-52.54 and 103.83-562.28 mg/kg,respectively.(3) The analysis results of correlation between average values of pH and contents of nutrient indexes in soils with different depths(0-15,15-30,30-45 cm) showed that the correlation of these indexes were different.(4)OM and total N contents in soils with different depths(0-15,15-30,30-45 cm) all had a significant positive correlation with latitude and negative correlation with longitude and altitude,and the correlation of available P and available K contents in surface soils(0-15 cm) with latitude,longitude and altitude were significant positive,significant negative and significant negative,respectively;moreover,longitude and latitude also showed a significant impact on soil available K contents with the depth of 30-45 cm.In addition,comprehensive analysis result of nutrient contents showed that N.tangutorum populations in Huaitou Tala Town had the highest fertility,and the fertility levels of N.tangutorum populations in Chaka Town and Wulan Keke Town were relatively lower. 展开更多
关键词 Soil nutrients Spatial variability Geographical location nitraria tangutorum Bobr. Tsaidam Basin
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白刺(Nitraria tangutorum)灌丛沙堆干沙层土壤水分动态及其对小雨事件的响应 被引量:1
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作者 李新乐 安景源 +3 位作者 王丹阳 刘雅婧 辛智鸣 吴波 《中国草地学报》 CSCD 北大核心 2022年第5期20-30,共11页
干沙层对抑制蒸发、保护沙漠地区的生态水具有重要作用。以乌兰布和沙漠东缘白刺灌丛沙堆不同部位的干沙层为研究对象,采用烘干称重法对降雨前后灌丛沙堆不同部位干沙层土壤水分进行连续测定,结果表明:(1)从土壤水分空间变异来看,0~10c... 干沙层对抑制蒸发、保护沙漠地区的生态水具有重要作用。以乌兰布和沙漠东缘白刺灌丛沙堆不同部位的干沙层为研究对象,采用烘干称重法对降雨前后灌丛沙堆不同部位干沙层土壤水分进行连续测定,结果表明:(1)从土壤水分空间变异来看,0~10cm干沙层土壤水分变化受降雨影响最为明显,4.3mm降雨能影响到0~5cm干沙层土壤水分,5.4mm和10.8mm降雨能影响到0~15cm干沙层土壤水分;(2)从土壤水分时间动态来看,与降雨前相比,降雨后干沙层土壤水分呈迅速增加-逐渐降低的变化趋势,在降雨后第1d增加最为明显,然后从降雨后第2d开始逐渐下降;(3)位于沙丘不同部位的干沙层土壤水分对降雨的响应存在显著差异,降雨后干沙层厚度变化速率表现为:阳坡中部>阳坡底部>阴坡底部>阴坡中部>坡顶。小降雨对白刺灌丛沙堆干沙层的消失和产生有重要影响,干沙层的形成是水分下渗和地表蒸发共同作用的结果。 展开更多
关键词 白刺灌丛沙堆 干沙层 土壤水分动态 小雨事件
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Synergistic combination of arbuscular mycorrhizal fungi and plant growth-promoting rhizobacteria modulates morpho-physiological characteristics and soil structure in Nitraria tangutorum bobr.Under saline soil conditions 被引量:2
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作者 Jing Pan CuiHua Huang +5 位作者 Fei Peng Tao Wang Jie Liao ShaoXiu Ma QuanGang You Xian Xue 《Research in Cold and Arid Regions》 CSCD 2022年第6期393-402,共10页
Nitraria tangutorum Bobr.,a typical xero-halophyte,can be used for vegetation restoration and reconstruction in arid and semiarid regions affected by salinity.However,global climate change and unreasonable human activ... Nitraria tangutorum Bobr.,a typical xero-halophyte,can be used for vegetation restoration and reconstruction in arid and semiarid regions affected by salinity.However,global climate change and unreasonable human activity have exacerbated salinization in arid and semi-arid regions,which in turn has led to the growth inhibition of halophytes,including N.tangutorum.Arbuscular mycorrhizal fungi(AMF)and plant growth-promoting rhizobacteria(PGPR)have the potential to improve the salt tolerance of plants and their adaptation to saline soil environments.In this study,the effects of single and combined inoculations of AMF(Glomus mosseae)and PGPR(Bacillus amyloliquefaciens FZB42)on N.tangutorum were evaluated in severe saline soil conditions.The results indicate that AMF and PGPR alone may not adapt well to the real soil environment,and cannot ensure the effect of either growth promotion or salt-tolerance induction on N.tangutorum seedlings.However,the combination of AMF and PGPR significantly promoted mycorrhizal colonization,increased biomass accumulation,improved morphological development,enhanced photosynthetic performance,stomatal adjustment ability,and the exchange of water and gas.Co-inoculation also significantly counteracted the adverse effect of salinity on the soil structure of N.tangutorum seedlings.It is concluded that the effectiveness of microbial inoculation on the salt tolerance of N.tangutorum seedlings depends on the functional compatibility between plants and microorganisms as well as the specific combinations of AMF and PGPR. 展开更多
关键词 nitraria tangutorum Bobr. Arbuscular mycorrhizal fungi Plant growth-promoting rhizobacteria Morphological development Photosynthesis physiology Soil structure
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Identification of Tibetan and Qiang Edible and Medicinal Herbs Cardamine tangutorum and Cardamine macrophylla
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作者 Bochao LI Xiaoxia CAI +2 位作者 Yuebu HAILAI Yifan TIAN Yuan LIU 《Medicinal Plant》 2024年第4期31-34,39,共5页
[Objectives]To study the original plants,characters,tissue structure,powder characteristics and thin-layer chromatography(TLC)characteristics of Cardamine tangutorum and Cardamine macrophylla as Tibetan and Qiang edib... [Objectives]To study the original plants,characters,tissue structure,powder characteristics and thin-layer chromatography(TLC)characteristics of Cardamine tangutorum and Cardamine macrophylla as Tibetan and Qiang edible and medicinal herbs,and to provide the basis for the identification of C.tangutorum and C.macrophylla.[Methods]The identification of C.macrophyll and C.tangutorum was carried out by original plant identification,character identification,microscopic identification and TLC identification.[Results]C.tangutorum and C.macrophylla can be distinguished according to the shape of rhizome and stem,the difference of stem leaves and leaflets,and the difference of flower color;there is no obvious difference between the characteristics of the shape and the powder;the thin layer chromatography shows that in the thin layer chromatography of C.tangutorum and C.macrophylla,spots with the same color are shown on the corresponding positions of the ground part and the reference substance quercetin;the underground part and the position corresponding to the reference substanceβ-sitosterol all show the same color spots.[Conclusions]This study provides a reference for the identification of C.tangutorum and C.macrophylla. 展开更多
关键词 Cardamine tangutorum Cardamine macrophylla Original plant Microscopic identification Thin layer chromatography(TLC)
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不同生境条件下泡泡刺(Nitraria sphaerocarpa)种群的空间格局及动态分析 被引量:43
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作者 李秋艳 何志斌 +1 位作者 赵文智 李启森 《中国沙漠》 CSCD 北大核心 2004年第4期484-488,共5页
应用生态空间理论(theoryofspatialecology)和地统计技术,分析了不同生境条件下泡泡刺种群的空间分布特征及其动态。结果表明:受气候条件的控制,泡泡刺种群是以斑块状格局形式存在。在较小的尺度上,生境条件起到了明显的作用,戈壁生境... 应用生态空间理论(theoryofspatialecology)和地统计技术,分析了不同生境条件下泡泡刺种群的空间分布特征及其动态。结果表明:受气候条件的控制,泡泡刺种群是以斑块状格局形式存在。在较小的尺度上,生境条件起到了明显的作用,戈壁生境中的泡泡刺灌丛沙堆趋向于斑块小、密度大、空间自相关距离短,而沙漠生境中的结果相反。另外,沙埋和地下水位下降不仅为泡泡刺种群拓宽了生态位,而且是该种群演化的驱动力。 展开更多
关键词 泡泡刺种群 分布格局 生境 空间自相关性
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腾格里沙漠东南缘不同生境白刺(Nitraria)灌丛沙堆的空间分布格局 被引量:31
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作者 贾晓红 李新荣 《环境科学》 EI CAS CSCD 北大核心 2008年第7期2046-2053,共8页
运用地统计学和数量生态学方法,从维持天然白刺灌丛稳定性和持久生态效益角度出发,对腾格里沙漠东南缘不同生境白刺灌丛沙堆的空间异质性进行了研究,分析了不同生境白刺固沙生态效益.结果表明,生境地下水埋深和白刺植株发育状况的差异... 运用地统计学和数量生态学方法,从维持天然白刺灌丛稳定性和持久生态效益角度出发,对腾格里沙漠东南缘不同生境白刺灌丛沙堆的空间异质性进行了研究,分析了不同生境白刺固沙生态效益.结果表明,生境地下水埋深和白刺植株发育状况的差异直接影响了白刺耐沙埋的能力,间接地影响了白刺沙堆的固沙效应.湖盆低地白刺种群的植株盖度、密度和生物量显著高于山前冲积扇区(p〈0.01),植株高度则相反,且无显著差异(p〉0.05).湖盆低地的半固定生境沙堆高度平均为1.20m,单个沙堆积沙体积平均为88.19 m^3,固定生境的山前冲积扇沙堆高度平均为1.14 m,单个沙堆积沙体积平均为33.16 m^3.不同生境条件下,白刺沙堆的斑块大小和分布存在明显差异(p〈0.01).湖盆低地的白刺灌丛沙堆趋向于大斑块、小密度,空间自相关距离较长,而山前冲积扇区相反.湖盆低地由自相关因素引起的沙堆大小和积沙体积空间异质性占主要部分,分别体现在1.2-84 m和1.2-505 m尺度;而山前冲积扇的随机因素引起的沙堆大小和积沙体积空间异质性占到100%,在100 m以下尺度该区白刺灌丛沙堆大小和积沙体积具有恒定的变异,其空间分布格局在这个尺度无规律体现呈随机分布. 展开更多
关键词 白刺沙堆 空间分布 固沙效应
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不同种源比拉底白刺(Nitraria billardieri)水分生理研究 被引量:5
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作者 段娜 郝玉光 +3 位作者 刘芳 徐军 陈海玲 王珩 《西南农业学报》 CSCD 北大核心 2016年第5期1075-1080,共6页
为给乌兰布和风沙区幼苗培育、树种选择以及植被生态建设提供理论依据和技术支撑,盆栽条件下采取控水方式进行水分胁迫处理,研究引自澳大利亚Hopetoun、Murray Bridge和Port Lincoln3个地区的比拉底白刺和中国唐古特白刺的水分生理,并... 为给乌兰布和风沙区幼苗培育、树种选择以及植被生态建设提供理论依据和技术支撑,盆栽条件下采取控水方式进行水分胁迫处理,研究引自澳大利亚Hopetoun、Murray Bridge和Port Lincoln3个地区的比拉底白刺和中国唐古特白刺的水分生理,并用模糊隶属函数法对其抗旱性进行综合评价。结果表明,3个不同种源比拉底白刺和唐古特白刺幼苗叶片含水量均随着干旱胁迫的加剧而降低,方差分析表明,Port Lincoln和Murray Bridge地区的比拉底白刺差异不显著,与Hopetoun比拉底白刺和中国唐古特白刺相比有显著性差异,且Murray Bridge比拉底白刺叶片含水量最高,唐古特白刺叶片含水量最低;随土壤水分含量的降低,比拉底白刺和唐古特白刺的束缚水/自由水比值和叶片水分饱和亏缺均逐渐上升,同一胁迫条件下,Murray Bridge比拉底白刺束缚水/自由水比值和叶片水分饱和亏缺分别达到最高和最低;离体叶片失水速率变化趋势基本保持一致,Murray Bridge白刺失水最慢,方差分析显示与其他白刺呈显著性差异。各种原地白刺叶片含水量与失水速率、水分饱和亏缺呈极显著负相关;水分饱和亏缺与束缚水/自由水比值、失水速率显著相关。根据抗旱隶属值,比拉底白刺和唐古特白刺的抗旱性由大到小的顺序为:Murray Bridge比拉底白刺>Port Lincoln比拉底白刺>Hopetoun比拉底白刺>唐古特白刺。 展开更多
关键词 干旱胁迫 比拉底白刺 水分生理 隶属函数
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白刺(NitrariasibiricaPall.)胚胎学的研究 1.小孢子和雄配子体的发育 被引量:2
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作者 李师翁 屠骊珠 《内蒙古大学学报(自然科学版)》 CAS CSCD 1990年第1期112-118,I001,3,共9页
花药药壁发育为基本型.成熟绒毡层细胞具双核,属分泌型,小孢子母细胞减数分裂为同时型,小孢子四分体排列为四面体形,单核小孢子第一次有丝分裂时,纺锤体始终与小孢子细胞长轴平行.生殖核先于营养核形成,胞质分裂极不对称,营养细胞与生... 花药药壁发育为基本型.成熟绒毡层细胞具双核,属分泌型,小孢子母细胞减数分裂为同时型,小孢子四分体排列为四面体形,单核小孢子第一次有丝分裂时,纺锤体始终与小孢子细胞长轴平行.生殖核先于营养核形成,胞质分裂极不对称,营养细胞与生殖细胞间具明显的、非肼胝质性质的壁.散粉时花粉粒为2细胞,细胞内贮藏丰富淀粉粒.花药各部分组织中始终富合淀粉粒,在小孢子及雄配子体发育过程中,淀粉的消长显示一定的功能相关规律.小孢子母细胞在减数分裂前期时开始积累肼胝质壁;刚释放的小孢子肼胝质荧光弱,液泡期小孢子具较强的肼胝质荧光. 展开更多
关键词 白刺 小孢发育 雄配子体 胚胎学
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白刺Nitraria sibirica Pall.胚胎学研究——4 果实和种子的发育解剖学 被引量:1
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作者 李师翁 屠骊珠 《内蒙古大学学报(自然科学版)》 CAS CSCD 1991年第3期389-395,共7页
本文报道白刺果实和种子的发育解剖及其发育过程中淀粉的变化。白刺果实为复合核果。由三心皮合生的三室子房发育而来。三个子房室中有一室不产生胚珠,所形成的两个胚珠中仅一个最后形成种子,另一胚珠中途退化。果壁由外果皮。中果皮和... 本文报道白刺果实和种子的发育解剖及其发育过程中淀粉的变化。白刺果实为复合核果。由三心皮合生的三室子房发育而来。三个子房室中有一室不产生胚珠,所形成的两个胚珠中仅一个最后形成种子,另一胚珠中途退化。果壁由外果皮。中果皮和内果皮组成。外果皮成熟时肉质可食;中果皮源于维管形成层,由纤维细胞构成。为成熟核果的果核部分;内果皮由约8层薄壁细胞构成,果实成熟时退化解体。种皮由内珠被、外珠被和最外层珠心细胞共同构成。胚珠在其发育过程中产生承珠盘和珠孔塞结构。 展开更多
关键词 白刺 胚胎学 果实 种子 发育解剖学
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麦宾草(Elymus tangutorum)栽培驯化研究初报 被引量:6
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作者 王彦龙 马玉寿 +6 位作者 施建军 董全民 王柳英 盛丽 孙小弟 杨时海 李世雄 《青海畜牧兽医杂志》 2012年第3期1-2,共2页
对当地野生草种麦宾草进行了驯化栽培的初步研究。结果表明:麦宾草在海拔高度3 800m左右的果洛地区在栽培条件下能完成整个生育期生长,栽培当年高度可达到16.0cm,盖度达到60%,第2年株高可达到110cm,盖度达到96%;栽培当年干草产量为1 266... 对当地野生草种麦宾草进行了驯化栽培的初步研究。结果表明:麦宾草在海拔高度3 800m左右的果洛地区在栽培条件下能完成整个生育期生长,栽培当年高度可达到16.0cm,盖度达到60%,第2年株高可达到110cm,盖度达到96%;栽培当年干草产量为1 266.8kg/hm2,第2年、第3年和第4年的干草产量分别为7 959.4kg/hm2,6 462.4kg/hm2,5 244.1 kg/hm2,种子产量分别为1 000.2kg/hm2,880.6kg/hm2,790.5kg/hm2。该草种是适合三江源区黑土滩退化草地植被恢复与重建的优良草种,也是高寒草甸地区建植高质量的生态型或刈用型黑土滩人工草地的优良草种。 展开更多
关键词 麦宾草 栽培驯化 黑土滩退化草地 三江源区
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Soil water repellency and influencing factors of Nitraria tangutorun nebkhas at different succession stages 被引量:11
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作者 HaoTian YANG XinRong LI +3 位作者 LiChao LIU YanHong GAO Gang LI RongLiang JIA 《Journal of Arid Land》 SCIE CSCD 2014年第3期300-310,共11页
Abstract: Soil water repellency (WR) is an important physical characteristic of soil surface. It is capable of largely influencing the hydrological and geomorphological processes of soil, as well as affecting the e... Abstract: Soil water repellency (WR) is an important physical characteristic of soil surface. It is capable of largely influencing the hydrological and geomorphological processes of soil, as well as affecting the ecological processes of plants, such as growth and seed germination, and has thus been a hot topic in recent research around the world. In this paper, the capillary rise method was used to study the soil WR characteristics of Nitraria tangutorun nebkhas. Soil water repellencies at different succession stages of Nitraria tangutorun were investigated, and the relationships between soil WR and soil organic matter, total N, and total P, soil texture, pH, and concentrations of CO32, HCO3-, CI, SO42-, Na~, K~, Ca2~ and Mg2+ were discussed. Soil WR may be demonstrated at the following nebkhas dune evolvement stages: extremely degraded〉degraded〉stabilized〉well developed〉newly developed〉quick sand. Apart from some soil at the bottom, the WR of other soils (crest and slope of dune) was found to be largest at the topsoil, and decreased as the soil depth increased. The results showed that multiple factors affected soil WR characteristics e.g. WR increased significantly as the contents of soil organic matter and total N increased, but did not change as the total P content increased. Soil texture was a key factor affecting soil WR; soil WR increased significantly as clay content increased, and decreased significantly as sand content increased. Low pH was shown to be more suitable for the occurrence of soil WR. Four cations (Ca2+, Mg2+, K+ and Na+) and two anions (CI and SO42) enhanced soil WR, while CO32-decreased it. HCO3- did not show any observable effect. Finally, we established a best-fit general linear model (GLM) between soil-air-water contact angle (CA) and influencing factors (CA=5.606 sand+6.496 (clay and silt)-2.353 pH+470.089 CQ2+11.346 Na+-407.707 Cl--14.245 SO42-+0.734 total N-519.521 ). It was concluded that all soils contain subcritical WR (0°〈CA〈90°). The development and succession of Nitraria tangutorun nebkhas may improve the formation of soil subcritical WR. There exist significant relationships between soils subcritical WR and soil physical or chemical properties. 展开更多
关键词 HYDROPHOBICITY soil-air-water contact angle capillary rise method nitraria tangutorun nebkhas vegetation succession stage
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Adaptation of Nitraria sphaerocarpa to wind-blown sand environments at the edge of a desert oasis 被引量:3
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作者 LI Qiu-yan ZHAO Wen-zhi FANG Hai-yan 《Journal of Environmental Sciences》 SCIE EI CAS CSCD 2007年第4期482-487,共6页
This study addresses the adaptation of Nitraria sphaerocarpa to blown sand at the edge of a desert oasis with regard to the aspects of soil seed banks, seedlings, and population. Horizontally, the total number of seed... This study addresses the adaptation of Nitraria sphaerocarpa to blown sand at the edge of a desert oasis with regard to the aspects of soil seed banks, seedlings, and population. Horizontally, the total number of seeds per unit area decreased from the shrub canopy center to intershrub areas, and most seeds were found under shrub canopies. Vertically, the highest proportion of seeds was found at depths of 5-10 cm. The emergence percentage, seedling mass, and seedling height, which were significantly affected by both burial depth and seed size, were highest at the optimal burial depth of 2 cm, and decreased with increasing burial depth in each seed size-class. Although seedling mass was usually greatest for large seeds and least for small seeds at each burial depth, little difference was observed in seedling height at shallow burial depths of 0-3 cm. The population shows a patchy and discontinuous distribution pattern. Population height increases with increasing sand depth. Also the density increases with increasing depth of sand in the desert; however, there is a steady decrease when the depth of sand is more than 100 cm. This result indicates that the depth of sand that is most suitable for the growth of Nitraria sphaerocarpa is 100 cm. The size of the population is significantly correlated with the sand depth, which increases with increasing depth in the desert. 展开更多
关键词 nitraria sphaerocarpa soil seed bank seedling growth sand burial ADAPTATION
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Nitraria sibirica cell suspension culture:establishment,characterization and application 被引量:1
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作者 Xindi Mei Yingbai Shen 《Journal of Forestry Research》 SCIE CAS CSCD 2017年第5期935-942,共8页
Nitraria sibirica Pall. is a shrub that grows in saline-alkali soil and has traditional medicinal value and potential commercial value. The objectives of this study include induction and multiplication of callus, esta... Nitraria sibirica Pall. is a shrub that grows in saline-alkali soil and has traditional medicinal value and potential commercial value. The objectives of this study include induction and multiplication of callus, establishment of a suspension cell line, and isolation of protoplasts from cell suspensions. Murashige and Skoog (MS) medium was used for callus induction from mature seeds of N. sibirica. Seed-derived calluses were further multiplied on MS medium augmented with 0.5 mg L-1 6-benzylaminopurine (6-BA) and 1.0 mg L-1 2,4-dichlorophenoxy (2,4-D) acetic acid. Suspension cultures of N. sibirica were initiated by transferring friable calli to the same liquid multiplication medium. Characterization of the suspension culture was assessed based on fresh mass, dry mass, cell viability and pH value of the culture. A typical growth curve was observed after inoculating 1.5 g of callus in 40 mL liquid medium, including a lag phase, an exponential growth phase, a stationary phase, and a negative acceleration phase. The effect of factors such as pre-plasmolysis, enzyme combination, enzymolysis time and mannitol concentration, on the isolation of cell-derived protoplasts were evaluated to determine the usefulness of suspension cultures. The maximum yield (9.79 x 10(6) cells/g) and highest viability (79.97%) of protoplast were reached when approximately 1 g of cell suspension (cultured for 6 days) was inoculated for 12 h in cell and protoplast washing solution made of 0.8 mol L-1 mannitol mixture solution, cellulose onozuka R-10 2% (w/v), hemicellulose 0.2%, macerozyme R-10 1%, and pectolyase Y-23 0.5%. Protoplast yield was significantly influenced by pre-plasmolysis and cellulose onozuka R-10 (P < 0.05). 展开更多
关键词 CALLUS Growth curve Protoplast isolation nitraria sibirica Suspension culture
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Effect of rain enrichment on soil respiration of Nitraria sphaerocarpa community in a hyperarid area 被引量:1
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作者 DianJun Liu Bo Wu +5 位作者 YongHua Li GuangHui Lin ShiPing Chen YaJuan Zhu Qi Lu Bin Yao 《Research in Cold and Arid Regions》 CSCD 2013年第6期722-732,共11页
In order to analyze the effect of rain enrichment on soil respiration rate of a Nitraria sphaerocarpa community, we measured soil respiration rate in bare and vegetated areas in a hyperarid area (Dunhuang) during th... In order to analyze the effect of rain enrichment on soil respiration rate of a Nitraria sphaerocarpa community, we measured soil respiration rate in bare and vegetated areas in a hyperarid area (Dunhuang) during the growing season. Results show that rain enrichment can increase bare and vegetated soil respiration rates. The more rainfall enrichment, the greater the increment and the longer duration time effect for soil respiration rate. 200% (16 mm) and 300% (24 mm) of rain enrichment can significantly increase bare soil respiration rates by 90% and 106% (P〈0.01), respectively. By contrast, areas with 100% (8 mm), 200% (16 mm) and 300% (24 mm) of rain enrichment can significantly increase shrub area respiration rates by 68%, 157% and 205% (P〈0.01), respectively. The response time of bare and vegetated soil respiration to rainfall enrichment is asynchronous. Response variable of soil respiration in vegetated soil is higher (118%) than in bare soil. There was significant positive correlation between soil respiration rate and soil water content during the growing season (P〈0.01). For every 1 mm increment of precipitation, soil respiration rate increased by 0.01 and 0.04 pmol/(m2.s), respectively in vegetated and bare soils. 展开更多
关键词 soil respiration rain enrichment nitraria sphaerocarpa soil water content
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Optimization of Ultrasonic-enzymatic Extraction of Total Flavonoids from Cardamine tangutorum O.E.Schulz by Response Surface Methodology and Content Determination 被引量:2
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作者 Xiaoxia CAI Bochao LI +3 位作者 Xuexue LI Hongna SU Wenbing LI Yuan LIU 《Medicinal Plant》 CAS 2020年第6期36-41,共6页
[Objectives]This study aimed to optimize the extraction process of total flavonoids in Cardamine tangutorum O.E.Schulz.[Methods]On the basis of single-factor experiments,the ultrasonic-enzymatic extraction process of ... [Objectives]This study aimed to optimize the extraction process of total flavonoids in Cardamine tangutorum O.E.Schulz.[Methods]On the basis of single-factor experiments,the ultrasonic-enzymatic extraction process of total flavonoids in C.tangutorum O.E.Schulz was optimized by Box-Behnken design from aspects of ethanol volume fraction,extraction time,extraction temperature and enzyme addition,with yield of total flavonoids as the evaluation index.[Results]The optimal extraction process of total flavonoids in C.tangutorum O.E.Schulz was as follows:ethanol volume fraction of 70%,ultrasonic extraction time of 43 min,ultrasonic extraction temperature of 37℃and cellulose addition of 0.07 mg.Under the optimal conditions,the yield of total flavonoids from C.tangutorum O.E.Schulz reached 75.90 mg/g.[Conclusions]The optimized extraction process was reasonable and feasible,and it could provide a reference for the extraction of total flavonoids in C.tangutorum O.E.Schulz.This study provided a foundation for the quality control of food/medicinal materials and the development and research of big health products of C.tangutorum O.E.Schulz. 展开更多
关键词 Cardamine tangutorum O.E.Schulz Total flavonoid Response surface methodology Ultrasonic-enzymatic hydrolysis Extraction process
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西藏麦宾草(Elymus tangutorum)种子特征研究
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作者 彭艳 索朗央措 +1 位作者 马素洁 魏学红 《高原农业》 2018年第4期360-365,共6页
种子特征与幼苗的生存、发育和植株未来的竞争密切相关。本研究对分布在林芝地区八一镇野生草种西藏麦宾草(Elymus tangutorum)种子为试验对象,通过观测麦宾草主要形态特征,并对其种子特征及其萌发特性进行了分析。结果表明:西藏麦宾草... 种子特征与幼苗的生存、发育和植株未来的竞争密切相关。本研究对分布在林芝地区八一镇野生草种西藏麦宾草(Elymus tangutorum)种子为试验对象,通过观测麦宾草主要形态特征,并对其种子特征及其萌发特性进行了分析。结果表明:西藏麦宾草的主杆长度、穗长、小穗数量的平均值分别为1354.65 mm、165.70 mm和51.85个,麦宾草种子扁平,圆形或半圆形,种子芒长介于1.0 mm^11.0 mm之间,平均值为8.0 mm,种子长度介于7.0 mm^14.0 mm之间,平均值为9.6 mm。通过对3个不同培养皿麦宾草种子千粒重、含水率及发芽率进行研究,发现西藏麦宾草种子千粒重(4.705 g、4.776 g、4.542 g)、含水率(11.65%,12.34%,10.49%)和发芽率(73.70%,78.89%,59.63%)的平均值分别为4.67g、11.49%、70.74%,根(12.33 mm/d,12.35 mm/d,10.16 mm/d)和苗(17.67 mm/d,16.79 mm/d,13.33 mm/d)的生长速率平均值分别为11.61 mm/d、15.93 mm/d。且均表现为2号培养皿>1号培养皿>3号培养皿。总之,麦宾草种子千粒重越大,相应的含水率、发芽率也相对较高且生命力旺盛,萌发之后根和苗的生长速率也相对较快,发育过程中生长势呈现良好状态。 展开更多
关键词 麦宾草 种子 形态特征 种子特征
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Determination and Multidimensional Analysis of 29 Inorganic Elements in Cardamine tangutorum O.E.Schulz of Different Origins
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作者 Xiaoxia CAI Bochao LI +4 位作者 Junzhang QUBIE Yuebu HAILAI Jianlong LAN Wenbing LI Yuan LIU 《Medicinal Plant》 CAS 2020年第6期12-15,23,共5页
[Objectives]This study aimed to determine the contents of inorganic elements in Cardamine tangutorum O.E.Schulz samples from different origins to provide a scientific reference for the quality control,safety evaluatio... [Objectives]This study aimed to determine the contents of inorganic elements in Cardamine tangutorum O.E.Schulz samples from different origins to provide a scientific reference for the quality control,safety evaluation and clinical medicinal use of C.tangutorum O.E.Schulz.[Methods]The contents of Al,As,B,Ba,Ca,Cd,Co,Cr,Cu,Fe,Hg,K,Mg,Mn,Mo,Na,Ni,P,Pb,Pd,Rb,Se,Si,Sn,Sr,Ti,Tl,V and Zn in the C.tangutorum O.E.Schulz samples were determined simultaneously by wet digestion and inductively coupled plasma optical emission spectroscopy(ICP-OES),and the determination results were analyzed by principal component analysis.[Results]A total of 28 inorganic elements were detected in the samples of C.tangutorum O.E.Schulz from different origins.The contents of heavy metals did not exceed the limits.Among the elements,the determined values of Hg in all samples were below the detection limit,so it was not detected.The contents of Ca,Al,P and Fe were relatively high,and they were essential nutrients in the human body.There were significant differences among different elements.Samples from different origins show similar patterns in the distribution of main elements.Correlation analysis shows that 15 pairs of inorganic elements out of 24 elements had extremely significant positive correlations,and 26 pairs of elements had significant positive correlations.The principal component analysis determined Al,As,Ba,Ca,Cd,Fe,Mn,Ni,P,Pb,Pd,Se,V and Zn were the characteristic inorganic elements in the samples of C.tangutorum O.E.Schulz.[Conclusions]The contents of toxic heavy metals in C.tangutorum O.E.Schulz samples from different origins met the edible requirements.The method was simple,rapid and accurate,and could be used to analyze and determine the inorganic elements in the samples of C.tangutorum O.E.Schulz,providing a theoretical basis for the better development and utilization of C.tangutorum O.E.Schulz. 展开更多
关键词 Cardamine tangutorum O.E.Schulz Inorganic element Inductively coupled plasma optical emission spectroscopy(ICP-OES) Principal component analysis
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基于MaxEnt模型的中国白刺属植物种群动态分析
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作者 姜生秀 胡静 +5 位作者 王方琳 胡小柯 王昱淇 邱晓娜 张珊 尉秋实 《中国野生植物资源》 CSCD 2024年第11期122-128,共7页
目的:了解气候变化情景下白刺属植物在中国的潜在分布和种群动态,为白刺属在中国的保护与恢复提供理论依据。方法:利用144个分布点和9个环境因子,通过MaxEnt模型,模拟白刺属在不同时期(末次冰盛期、末次间冰期、当代和未来)在中国的适... 目的:了解气候变化情景下白刺属植物在中国的潜在分布和种群动态,为白刺属在中国的保护与恢复提供理论依据。方法:利用144个分布点和9个环境因子,通过MaxEnt模型,模拟白刺属在不同时期(末次冰盛期、末次间冰期、当代和未来)在中国的适生区及迁移路线,并分析影响其分布的气候因素。结果:最暖季度的降水量(bio18)、年均温(bio1)、温度季节性变化标准差(bio4)和最冷季平均温(bio11)对白刺属分布有重要影响,其适宜范围分别为:50~250 mm、11~22.5℃、600~1400和-12.5~-2.5℃。当代气候情景下,白刺属适生区在中国西北各地区均有分布,在陕西和山西也有少量分布,适生区总面积为277.17×10^(4)km^(2),高适生区为68.92×10^(4)km^(2),中适生区为90.03×10^(4)km^(2),低适生区为118.22×10^(4)km^(2)。相比当代,末次冰盛期、末次间冰期和未来3个时期的高、中、低适生区面积均有所减少,另外,从末次冰盛期到末次间冰期,白刺属适生区总面积有所扩张。质心分析表明,在未来,白刺属有向低海拔处迁移的趋势。结论:在未来全球气候变暖的大背景下,白刺属植物将面临生存风险,应加强白刺属植物的保护和利用。 展开更多
关键词 白刺属 MaxEnt模型 环境因子 潜在分布区 种群动态
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3种寄主植物对白刺夜蛾生长发育、繁殖和寿命的影响
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作者 胡桂馨 勾文山 +3 位作者 马维新 孔建宏 唐玲 孙尧德 《植物保护》 CAS CSCD 北大核心 2024年第1期110-115,136,共7页
白刺夜蛾Leiometopon simyrides是荒漠植物白刺的重要害虫,为明确寄主植物对白刺夜蛾生长发育的影响,以便对其进行科学监测和防控,本研究在27℃下,利用3种寄主植物(大果白刺、泡泡刺、唐古特白刺)饲养白刺夜蛾,观察记录白刺夜蛾幼虫的... 白刺夜蛾Leiometopon simyrides是荒漠植物白刺的重要害虫,为明确寄主植物对白刺夜蛾生长发育的影响,以便对其进行科学监测和防控,本研究在27℃下,利用3种寄主植物(大果白刺、泡泡刺、唐古特白刺)饲养白刺夜蛾,观察记录白刺夜蛾幼虫的取食量、各阶段的发育历期、存活率、羽化率、雌雄性比和成虫寿命等生物学特性,同时测定各寄主植物叶片的营养和次生代谢物质含量。结果表明,白刺夜蛾幼虫取食大果白刺叶片的总量最少,取食唐古特白刺的量最多。取食唐古特白刺的白刺夜蛾幼虫发育历期最短,其次为取食泡泡刺,取食大果白刺时历期最长。取食泡泡刺和唐古特白刺后,白刺夜蛾幼虫的存活率和羽化率较高,取食大果白刺存活率最低,且无成虫羽化。在唐古特白刺上,白刺夜蛾雌虫占比较高,成虫寿命较长。大果白刺的叶片总酚含量和简单酚含量最高,各营养物质含量最低,不利于白刺夜蛾的生长发育;唐古特白刺和泡泡刺酚类物质含量较低,营养物质含量较高,较适宜白刺夜蛾的生长发育和繁殖。唐古特白刺为白刺夜蛾的最适宜寄主植物,其次为泡泡刺。 展开更多
关键词 白刺 白刺夜蛾 生长发育 取食量 营养物质 次生代谢物质
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