Comprehensive Summary,Diabetic wound healing is threatening worldwide and challenging in wound dressings.Aiming to inhibit heavy inflammation and bacterial infection in diabetic wounds,herein,we facilely construct a k...Comprehensive Summary,Diabetic wound healing is threatening worldwide and challenging in wound dressings.Aiming to inhibit heavy inflammation and bacterial infection in diabetic wounds,herein,we facilely construct a kind of NO-releasing poly(L-glutamic acid)(PGA)based graphene oxide(GO)hybrid hydrogel of HDGS at a lower solid percentage,presenting large microporous size of about 20μm,mild photothermal conversion property,and good biocompatibility.Besides improving hemostasis performance,the less amount of GO endowed the hydrogel with near infrared(NIR)responsivity to control fast and pulsatile NO release for killing bacteria and inhibiting heavy inflammation to proheal diabetic wound,in which a broad spectrum of antibacterial activities toward killing S.aureus,E.coli and MRSA was achieved via a combined effect of photothermia and NO release.In vivo effective hemostasis was attained in a rat liver bleeding model with short hemostatic time of~20 s and lower blood loss of 1.5%—2.0%.Moreover,the treatment of HDGS plus 4 times of mild NIR irradiation(10 min,808 nm,1 W/cm^(2)per time)performed superior full diabetic wound healing within 11—14 d,in which the regenerated skins were characteristic of thick epidermis/dermis,dense blood vessels,some hair follicles-embedding,and high level of collagens.展开更多
Severe burns are challenging to heal and result in significant death throughout the world.Adiposederived mesenchymal stem cells(ADSCs)have emerged as a promising treatment for fullthickness burn healing but are impede...Severe burns are challenging to heal and result in significant death throughout the world.Adiposederived mesenchymal stem cells(ADSCs)have emerged as a promising treatment for fullthickness burn healing but are impeded by their low viability and efficiency after grafting in vivo.Nitric oxide(NO)is beneficial in promoting stem cell bioactivity,but whether it can function effectively in vivo is still largely unknown.In this study,we bioprinted an efficient biological scaffold loaded with ADSCs and NO(3D-ADSCs/NO)to evaluate its biological efficacy in promoting severe burn wound healing.The integral 3D-ADSCs/NO hydrogel scaffolds were constructed via 3D bioprinting.Our results shown that 3D-ADSCs/NO can enhance the migration and angiogenesis of Human Umbilical Vein Endothelial Cells(HUVECs).Burn wound healing experiments in mice revealed that 3D-ADSCs/NO accelerated the wound healing by promoting faster epithelialization and collagen deposition.Notably,immunohistochemistry of CD31 suggested an increase in neovascularization,supported by the upregulation of vascular endothelial growth factor(VEGF)mRNA in ADSCs in the 3D biosystem.These findings indicated that 3D-ADSC/NO hydrogel scaffold can promote severe burn wound healing through increased neovascularization via the VEGF signalling pathway.This scaffold may be considered a promising strategy for healing severe burns.展开更多
Nitric oxide(NO)has emerged as a potential wound therapeutic agent due to its pivotal role in the wound healing processes.Nevertheless,NO-based therapy for clinical applications is still restricted due to its gaseous ...Nitric oxide(NO)has emerged as a potential wound therapeutic agent due to its pivotal role in the wound healing processes.Nevertheless,NO-based therapy for clinical applications is still restricted due to its gaseous state and short half-life.Here we exploited a wound dressing by incorporating sodium nitroprusside doped prussian blue nanoparticals and Type I collagen into the chitosan/poly(vinyl alcohol)nanofibers through the electrospinning method.This hybrid nanofibrous scaffold possess the excellent abilities of NIR controlled NO release,photothermal therapy,and imitation of extra-cellular matrix-like architecture.These synergistic effects could enhance their anti-bactericidal effects in vitro and furthermore accelerate wound healing in vivo when compared to control groups.Histological analysis demonstrated the scaffold could promote fibroblast growth and accelerate epithelialization.Moreover,no apparent histological toxicology and negligible damage to major organs were observed,which provided sufficient biosafety for in vivo application.These data indicate the fabricated hybrid nanofibrous scaffold could be used as an ideal candidate for accelerating wound healing and treating chronic wounds.展开更多
基金financial support by National Natural Science Foundation of China(22075176)Natural Science Foundation of Shanghai(22ZR1429200).
文摘Comprehensive Summary,Diabetic wound healing is threatening worldwide and challenging in wound dressings.Aiming to inhibit heavy inflammation and bacterial infection in diabetic wounds,herein,we facilely construct a kind of NO-releasing poly(L-glutamic acid)(PGA)based graphene oxide(GO)hybrid hydrogel of HDGS at a lower solid percentage,presenting large microporous size of about 20μm,mild photothermal conversion property,and good biocompatibility.Besides improving hemostasis performance,the less amount of GO endowed the hydrogel with near infrared(NIR)responsivity to control fast and pulsatile NO release for killing bacteria and inhibiting heavy inflammation to proheal diabetic wound,in which a broad spectrum of antibacterial activities toward killing S.aureus,E.coli and MRSA was achieved via a combined effect of photothermia and NO release.In vivo effective hemostasis was attained in a rat liver bleeding model with short hemostatic time of~20 s and lower blood loss of 1.5%—2.0%.Moreover,the treatment of HDGS plus 4 times of mild NIR irradiation(10 min,808 nm,1 W/cm^(2)per time)performed superior full diabetic wound healing within 11—14 d,in which the regenerated skins were characteristic of thick epidermis/dermis,dense blood vessels,some hair follicles-embedding,and high level of collagens.
基金This work was supported by the Natural Science Foundation of Guangdong Province,China(No.2017A030313889)This work was partly supported by National Natural Science Foundation of China(No.81772368)the Science and Technology Planning Project of Guangdong Province(No.2017B090912007).
文摘Severe burns are challenging to heal and result in significant death throughout the world.Adiposederived mesenchymal stem cells(ADSCs)have emerged as a promising treatment for fullthickness burn healing but are impeded by their low viability and efficiency after grafting in vivo.Nitric oxide(NO)is beneficial in promoting stem cell bioactivity,but whether it can function effectively in vivo is still largely unknown.In this study,we bioprinted an efficient biological scaffold loaded with ADSCs and NO(3D-ADSCs/NO)to evaluate its biological efficacy in promoting severe burn wound healing.The integral 3D-ADSCs/NO hydrogel scaffolds were constructed via 3D bioprinting.Our results shown that 3D-ADSCs/NO can enhance the migration and angiogenesis of Human Umbilical Vein Endothelial Cells(HUVECs).Burn wound healing experiments in mice revealed that 3D-ADSCs/NO accelerated the wound healing by promoting faster epithelialization and collagen deposition.Notably,immunohistochemistry of CD31 suggested an increase in neovascularization,supported by the upregulation of vascular endothelial growth factor(VEGF)mRNA in ADSCs in the 3D biosystem.These findings indicated that 3D-ADSC/NO hydrogel scaffold can promote severe burn wound healing through increased neovascularization via the VEGF signalling pathway.This scaffold may be considered a promising strategy for healing severe burns.
基金NNSFC(21901186,82004163)NSF of Shandong Province(ZR2019BB032,ZR2020MH400,ZR2020QH324)for financial support。
文摘Nitric oxide(NO)has emerged as a potential wound therapeutic agent due to its pivotal role in the wound healing processes.Nevertheless,NO-based therapy for clinical applications is still restricted due to its gaseous state and short half-life.Here we exploited a wound dressing by incorporating sodium nitroprusside doped prussian blue nanoparticals and Type I collagen into the chitosan/poly(vinyl alcohol)nanofibers through the electrospinning method.This hybrid nanofibrous scaffold possess the excellent abilities of NIR controlled NO release,photothermal therapy,and imitation of extra-cellular matrix-like architecture.These synergistic effects could enhance their anti-bactericidal effects in vitro and furthermore accelerate wound healing in vivo when compared to control groups.Histological analysis demonstrated the scaffold could promote fibroblast growth and accelerate epithelialization.Moreover,no apparent histological toxicology and negligible damage to major organs were observed,which provided sufficient biosafety for in vivo application.These data indicate the fabricated hybrid nanofibrous scaffold could be used as an ideal candidate for accelerating wound healing and treating chronic wounds.
基金国家自然科学基金(3090180330728021)+3 种基金高等学校博士学科点专项科研基金(20090001120046)北京大学中央高校基本科研业务费NIH R01 GM077352American Diabetes Association Research Award 7-08-RA-23资助课题
