Cucumber and rice plants with varying ammonium (NH4+) sensitivities were used to examine the effects of different nitrogen (N) sources on gas exchange, chlorophyll (Chl) fluorescence quenching, and photosynthetic elec...Cucumber and rice plants with varying ammonium (NH4+) sensitivities were used to examine the effects of different nitrogen (N) sources on gas exchange, chlorophyll (Chl) fluorescence quenching, and photosynthetic electron allocation. Compared to nitrate (NO3-)-grown plants, cucumber plants grown under NH4+-nutrition showed decreased plant growth, net photosynthetic rate, stomatal conductance, intercellular carbon dioxide (CO2) level, transpiration rate, maximum photochemical efficiency of photosystem II, and O2-independent alternative electron flux, and increased O2-dependent alternative electron flux. However, the N source had little effect on gas exchange, Chl a fluorescence parameters, and photosynthetic electron allocation in rice plants, except that NH4+-grown plants had a higher O2-independent alternative electron flux than NO3--grown plants. NO3- reduction activity was rarely detected in leaves of NH4+-grown cucumber plants, but was high in NH4+-grown rice plants. These results demonstrate that significant amounts of photosynthetic electron transport were coupled to NO3- assimilation, an effect more significant in NO3-- grown plants than in NH4+-grown plants. Meanwhile, NH4+-tolerant plants exhibited a higher demand for the reduced form of nicotinamide adenine dinucleotide phosphate (NADPH) for NO3- reduction, regardless of the N form supplied, while NH4+-sensitive plants had a high water-water cycle activity when NH4+ was supplied as the sole N source.展开更多
基金supported by the National Basic Research Program (973) of China (No. 2009CB119000)the National High-Tech R&D Program (863) of China (No. 2008BADA6B02)the National Natural Science Foundation of China (Nos. 30771471 and 30972033)
文摘Cucumber and rice plants with varying ammonium (NH4+) sensitivities were used to examine the effects of different nitrogen (N) sources on gas exchange, chlorophyll (Chl) fluorescence quenching, and photosynthetic electron allocation. Compared to nitrate (NO3-)-grown plants, cucumber plants grown under NH4+-nutrition showed decreased plant growth, net photosynthetic rate, stomatal conductance, intercellular carbon dioxide (CO2) level, transpiration rate, maximum photochemical efficiency of photosystem II, and O2-independent alternative electron flux, and increased O2-dependent alternative electron flux. However, the N source had little effect on gas exchange, Chl a fluorescence parameters, and photosynthetic electron allocation in rice plants, except that NH4+-grown plants had a higher O2-independent alternative electron flux than NO3--grown plants. NO3- reduction activity was rarely detected in leaves of NH4+-grown cucumber plants, but was high in NH4+-grown rice plants. These results demonstrate that significant amounts of photosynthetic electron transport were coupled to NO3- assimilation, an effect more significant in NO3-- grown plants than in NH4+-grown plants. Meanwhile, NH4+-tolerant plants exhibited a higher demand for the reduced form of nicotinamide adenine dinucleotide phosphate (NADPH) for NO3- reduction, regardless of the N form supplied, while NH4+-sensitive plants had a high water-water cycle activity when NH4+ was supplied as the sole N source.
