Impact of Different Rates of Nitrogen Supplementation on Soil PhysicochemicalProperties and Microbial Diversity in Goji Berry

Goji berry(Lycium barbarum L.)is substantially dependent on nitrogen fertilizer application,which can signifi-cantly enhance fruit yield and Goji berry industrial development in Ningxia,China.This study aimed to analyze the functions of differential nitrogen application rates including low(N1),medium(N2),and high(N3)levels in soil microbial community structure(bacterial and fungal)at 2 diverse soil depths(0-20,20-40 cm)through high-throughput sequencing technology by targeting 16S RNA gene and ITS1&ITS2 regions.All the observed physicochemical parameters exhibited significant improvement(p<0.05)with increased levels of nitrogen and the highest values for most parameters were observed at N2.However,pH decreased(p<0.05)gradually.The alpha and beta diversity analyses for bacterial and fungal communities’metagenome displayed more similarities than differences among all groups.The top bacterial and fungal phyla and genera suggested no obvious(p>0.05)differences among three group treatments(N1,N2,and N3).Furthermore,the functional enrichment analysis demonstrated significant(p<0.05)enrichment of quorum sensing,cysteine and methionine metabolism,and transcriptional machinery for bacterial communities,while various saprotrophic functional roles for fungal communities.Conclusively,moderately reducing the use of N-supplemented fertilizers is conducive to increasing soil nitrogen utilization rate,which can contribute to sustainable agriculture practices through improved soil quality,and microbial community structure and functions.

Facile preparation of Ag_(2)S/KTa_(0.5)Nb_(0.5)O_(3) heterojunction for enhanced performance in catalytic nitrogen fixation via photocatalysis and piezo-photocatalysis

In this work, a novel heterojunction composite Ag_(2)S/KTa_(x)Nb_(1-x)O_(3)was designed and synthesized through a combination of hydrothermal and precipitation procedures. The Ta/Nb ratio of the KTa_(x)Nb_(1-x)O_(3)and the Ag_(2)S content were optimized. The best 0.5% Ag_(2)S/KTa_(0.5)Nb_(0.5)O_(3)(KTN) sample presents an enhanced photocatalytic performance in ammonia synthesis than KTN and Ag_(2)S. Under simulated sunlight, the NH_(3)generation rate of 0.5% Ag_(2)S/KTN reaches 2.0 times that of pure KTN. Under visible light, the reaction rate ratio of the two catalysts is 6.0.XRD, XPS, and TEM analysis revealed that Ag2S was intimately decorated on the KTN nanocubes surface, which promoted the electron transfer between the two semiconductors. The band structure investigation indicated that the Ag_(2)S/KTN heterojunction established a type-Ⅱ band alignment with intimate contact, thus realizing the effective transfer and separation of photogenerated carriers. The change in charge separation was considered as the main reason for the enhanced photocatalytic performance. Interestingly, the Ag_(2)S/KTN composite exhibited higher NH3generation performance under the combined action of ultrasonic vibration and simulated sunlight. The enhanced piezo-photocatalytic performance can be ascribed that the piezoelectric effect of KTN improved the bulk separation of charge carriers in KTN. This study not only provides a potential catalyst for photocatalytic nitrogen fixation but also shows new ideas for the design of highly efficient catalysts via semiconductor modification and external field coupling.

选择性催化氧化H_(2)S的富氮碳催化剂研究进展

近年来,能高效净化H_(2)S并回收硫资源的选择性催化氧化技术(H_(2)S-SCO)受到了广泛关注.开发高性能、高选择性及低成本的催化剂是H_(2)S-SCO技术的研究重点,其中富氮碳基催化剂(RNCC)具有高活性、无金属、易制备、低成本且易再生等优点,被认为是一类极具潜力的H_(2)S-SCO催化剂.本文介绍了金属基催化剂的发展,总结和归纳RNCC的制备方法、催化活性和物化性质,系统地讨论RNCC的构-效关系及影响RNCC性能的关键因素,并总结了RNCC的H_(2)S-SCO反应机理.最后指出RNCC目前存在机遇和挑战,并展望了未来的发展方向.

Corchorus olitorius: A Promising Medicinal Plant in Southern Africa and Effects of Growing Conditions on Its Bioactive Compounds—A Review

Corchorus olitorius (Jew’s mallow), is one of the African indigenous leafy vegetables increasingly getting attention as a possible contributor of both micronutrients and bioactive compounds including proteins, lipids, fiber and vitamin C to human nutrition. Leaves of Corchorus olitorius have been found to have high level of phytochemicals: flavonoids, polyphenols, tannins, and saponins that possess strong radical scavenging activity and antioxidant power. In the arid and semi-arid areas of the world, drought is the main limiting factor affecting plant productivity and influences almost all aspects of plant biology. Water stress deficit is known to cause oxidative stress condition that has generally been reported to elevate phenolic antioxidants in various crops including Jew’s mallow. On the other hand, fertilization is crucial for crop management and high yield, it also affects nutritional value of the food plants. Nitrogen (N) fertilization affects health and nutritional value, including mineral content, fatty acid profile, anti-oxidative capacity and polyphenol levels and composition. The possible effects of fertilization should be considered when deciding on fertilization regime, to optimize both plant physiology, productivity and food-related effects. Nitrogen is an important element for Jew’s mallow production since it responds well to it. However, appropriate amounts of nutrients need to be provided to crops at the right time to favor both crop growth, yield and quality. Different reports confirmed that addition or increase of N, negatively affects the total phenolics and total flavonoids, and reduces accumulation of defense-related secondary metabolites resulting in lower oxidative capacity. Increased secondary metabolite production during water deficit and low nitrogen in the soil has been reported as a stress mechanism by most plants. However, further research is required to explore the biochemical response of Jew’s mallow to water deficit and nitrogen fertilization.

S型控释氮肥运筹对水稻产量形成及氮素利用特征的影响

缓控释肥具有施用次数少、肥料利用率高的特点,S型热固性树脂包膜控释氮肥(S型控释氮肥)受土壤pH影响小、肥料稳定性好。本研究以S型控释氮肥为试材,分别设置了6个水稻品种的响应比较、4种氮肥施用量、5种氮肥施用时期及7种氮肥配施比例的4个盆栽试验,研究其对水稻产量形成、氮素吸收利用等的影响。结果表明:1)品种比较试验中,S型控释氮肥提高了6个水稻品种的产量,增幅在2.95%~18.57%,平均增加12.60%;产量提高的原因是穗数或每穗颖花数的增加;S型缓控释氮肥可显著提高供试水稻成熟期的氮素积累量、氮肥利用率,但氮素籽粒生产效率有所下降。2)控释肥运筹试验中,随着S型控释氮肥施用量和施用比例的增加,武运粳24号的产量呈先增加后下降的趋势,分别以每盆3g纯氮处理和控释氮肥占比80%处理产量增幅最大,作基肥时增产效应更显著;成熟期氮素积累量呈增加和先增加后下降的趋势,分别以每盆4.5 g纯氮处理和控释氮肥占比80%处理增幅最大,作基肥时增幅效应更显著;氮素籽粒生产效率和氮肥利用率呈降低趋势,氮素籽粒生产效率分别以控释氮肥占比0%和控释肥作保花肥最大,氮肥利用率分别以控释氮肥占比80%和控释肥作基肥时最大。3)控释肥对根系性状影响试验中,S型控释氮肥处理提高了武运粳24号水稻栽后30天单株不定根总长、根干重与吸收面积,提高了抽穗期单株不定根总长、根干重和根活力;单株不定根数、不定根总长、根干重、根活力的增加是产量、氮素积累量和氮肥利用率协同提高的根系基础。总之,S型控释氮肥可显著提高水稻的产量和氮肥利用率,作为基肥时最优。每盆3 g纯氮处理、控释氮肥占比80%左右时产量、成熟期氮素积累量、氮肥利用率协同增加,与其栽后30天、抽穗期根系性状优化有密切关系。

Change Trend of Chinese Urban Residents' Food-nitrogen Consumption

Change trend of Chinese urban residents＇ per capita food-nitrogen annual consumption from 1981 to 2007 was analyzed and predicted by using ARIMA time-series model in order to reveal the change of urban food-nitrogen consumption during the China＇s urbanization process.Results showed that after 1980s,the annual consumption of Chinese urban residents＇ food-nitrogen had a change trend of ＂ increase-decrease-increase＂ and generally presented as a slight increasing trend;With the acceleration of rapid economic development and urbanization process,Chinese urban residents＇ food-nitrogen consumption will still keep a rising trend in future,and also has a large rising space.

S/N对硫化物型自养反硝化性能及NO-2-N积累的影响

以Na 2S为硫源,采用批次实验探究不同初始S/N(摩尔比)对硫化物自养反硝化性能及NO_(2)^(-)-N积累的影响。结果表明,当S/N≤2.80时,NO_(3)^(-)-N及NO-X-N(NO_(2)^(-)-N+NO_(3)^(-)-N)去除效果随S/N的升高而显著提升,S/N由0.35升高到2.80时,前6 h内NO_(3)^(-)-N及NO-X-N去除率分别提高了3.7倍及3.6倍,当S/N继续增至4.20时,NO_(3)^(-)-N及NO-X-N去除效果较S/N为2.80时变化不大;当S/N≤0.70时系统内可以获得一定的NO_(2)^(-)-N积累,反应结束时NO_(2)^(-)-N积累率(0.35,0.7)分别可达47.9%及24.5%,当S/N≥1.40时难以稳定积累NO_(2)^(-)-N,NO_(2)^(-)-N最高积累率(1.40,2.80,4.20)分别为55.0%,33.3%,39.7%。S/N≤0.70时可获得一定的NO_(2)^(-)-N积累,而S/N在1.40~2.80范围内时,升高S/N可以提供更多电子用于反硝化,缓解各种反硝化酶对于电子的竞争,降低中间产物积累,提升脱氮效果。

Influence of nitrogen and sulfur fertilization on quality of canola(Brassica napus L.) under rainfed conditions

Field experiments were conducted at Cereal Crops Research Institute, Pirsabak, Nowshera, Pakistan, during winter 2003～2004; 2004～2005 to evaluate the effect of nitrogen; sulfur levels; methods of nitrogen application on canola (Brassica napus L. cv. Bulbul-98) under rainfed conditions. Four levels of S (0, 10, 20,; 30 kg/ha); three levels of N (40, 60,; 80 kg/ha); a control treatment with both nutrients at zero level were included in the experiments. Sulfur levels were applied at sowing while N levels were applied by three methods (100% soil application, 90% soil+10% foliar application,; 80% soil +20% foliar application). The experiments were laid out in randomized complete block (RCB) design having four replications. Oil content increased significantly up to 20 kg S/ha but further increase in S level did not enhance oil content. Glucosinolate content increased from 13.6 to 24.6 μmol/g as S rate was increased from 0 to 30 kg/ha. Protein content increased from 22.4% to 23.2% as S rate was increased from 0 to 20 kg/ha. Oil content responded negatively to the increasing N levels. The highest N level resulted in the highest values for protein (23.5%); glucosinolate (19.9 μmol/g) contents. Methods of N application had no significant impact on any parameters under study.

Effects of reclaimed water irrigation and nitrogen fertilization on the chemical properties and microbial community of soil

The ecological effect of reclaimed water irrigation and fertilizer application on the soil environment is receiving more attention.Soil microbial activity and nitrogen（N）levels are important indicators of the effect of reclaimed water irrigation on environment.This study evaluated soil physicochemical properties and microbial community structure in soils irrigated with reclaimed water and receiving varied amounts of N fertilizer.The results indicated that the reclaimed water irrigation increased soil electrical conductivity（EC）and soil water content（SWC）.The N treatment has highly significant effect on the ACE,Chao,Shannon（H）and Coverage indices.Based on a 16S ribosomal RNA（16S rRNA）sequence analysis,the Proteobacteria,Gemmatimonadetes and Bacteroidetes were more abundant in soil irrigated with reclaimed water than in soil irrigated with clean water.Stronger clustering of microbial communities using either clean or reclaimed water for irrigation indicated that the type of irrigation water may have a greater influence on the structure of soil microbial community than N fertilizer treatment.Based on a canonical correspondence analysis（CCA）between the species of soil microbes and the chemical properties of the soil,which indicated that nitrate N（NO3-–-N）and total phosphorus（TP）had significant impact on abundance of Verrucomicrobia and Gemmatimonadetes,meanwhile the p H and organic matter（OM）had impact on abundance of Firmicutes and Actinobacteria significantly.It was beneficial to the improvement of soil bacterial activity and fertility under 120 mg kg^-1 N with reclaimed water irrigation.

An entirely new approach based on remote sensing data to calculate the nitrogen nutrition index of winter wheat

The nitrogen nutrition index(NNI)is a reliable indicator for diagnosing crop nitrogen(N)status.However,there is currently no specific vegetation index for the NNI inversion across multiple growth periods.To overcome the limitations of the traditional direct NNI inversion method(NNI_(T1))of the vegetation index and traditional indirect NNI inversion method(NNI_(T2))by inverting intermediate variables including the aboveground dry biomass(AGB)and plant N concentration(PNC),this study proposed a new NNI remote sensing index(NNI_(RS)).A remote-sensing-based critical N dilution curve(Nc_(_RS))was set up directly from two vegetation indices and then used to calculate NNI_(RS).Field data including AGB,PNC,and canopy hyperspectral data were collected over four growing seasons(2012–2013(Exp.1),2013–2014(Exp.2),2014–2015(Exp.3),2015–2016(Exp.4))in Beijing,China.All experimental datasets were cross-validated to each of the NNI models(NNI_(T1),NNI_(T2)and NNI_(RS)).The results showed that:(1)the NNI_(RS)models were represented by the standardized leaf area index determining index(sLAIDI)and the red-edge chlorophyll index(CI_(red edge))in the form of NNI_(RS)=CI_(red edge)/(a×sLAIDI~b),where"a"equals 2.06,2.10,2.08 and 2.02 and"b"equals 0.66,0.73,0.67 and 0.62 when the modeling set data came from Exp.1/2/4,Exp.1/2/3,Exp.1/3/4,and Exp.2/3/4,respectively;(2)the NNI_(RS)models achieved better performance than the other two NNI revised methods,and the ranges of R2 and RMSE were 0.50–0.82 and 0.12–0.14,respectively;(3)when the remaining data were used for verification,the NNI_(RS)models also showed good stability,with RMSE values of 0.09,0.18,0.13 and 0.10,respectively.Therefore,it is concluded that the NNI_(RS)method is promising for the remote assessment of crop N status.

Efficacy and safety of liquid nitrogen cryotherapy for treatment of Barrett's esophagus

AIM To evaluate the efficacy and safety of liquid nitrogen cryotherapy as a primary or rescue treatment for BE,with and without dysplasia,or intramucosal adenocarcinoma (IMC).METHODS This was a retrospective,single-center study carried out in a tertiary care center including 45 patients with BE who was treatment-na?ve or who had persistent intestinal metaplasia(IM),dysplasia,or IMC despite prior therapy.Barrett's mucosa was resected via EMR when clinically appropriate,then patients underwent cryotherapy until eradication or until deemed to have failed treatment.Surveillance biopsies were taken at standard intervals.RESULTS From 2010 through 2014,33 patients were studied regarding the efficacy of cryotherapy.Overall,29 patients (88%) responded to cryotherapy,with 84% having complete regression of all dysplasia and cancer.Complete eradication of cancer and dysplasia was seen in 75% of subjects with IMC; the remaining two subjects did not respond to cryotherapy.Following cryotherapy,15 patients with high-grade dysplasia (HGD) had 30% complete regression,50% IM,and 7% low-grade dysplasia (LGD); one subject had persistent HGD.Complete eradication of dysplasia occurred in all 5 patients with LGD.In 5 patients with IM,complete regression occurred in 4,and IM persisted in one.In 136 cryotherapy sessions amongst 45 patients,adverse events included chest pain (1%),stricture (4%),and one gastrointestinal bleed in a patient on dual antiplatelet therapy who had previously undergone EMR.CONCLUSION Cryotherapy is an efficacious and safe treatment modality for Barrett's esophagus with and without dysplasia or intramucosal adenocarcinoma.

Dual-site collaboration boosts electrochemical nitrogen reduction on Ru-S-C single-atom catalyst

Electrocatalytic reduction of nitrogen into ammonia(NH_(3))is a highly attractive but challenging route for NH_(3)production.We propose to realize a synergetic work of multi reaction sites to overcome the limitation of sustainable NH_(3)production.Herein,using ruthenium-sulfur-carbon(Ru-S-C)catalyst as a prototype,we show that the Ru/S dual-site cooperates to catalyse eletrocatalytic nitrogen reduction reaction(eNRR)at ambient conditions.With the combination of theoretical calculations,in situ Raman spectroscopy,and experimental observation,we demonstrate that such Ru/S dual-site cooperation greatly facilitates the activation and first protonation of N_(2)in the rate-determining step of eNRR.As a result,Ru-S-C catalyst exhibits significantly enhanced eNRR performance compared with the routine Ru-N-C catalyst via a single-site catalytic mechanism.We anticipate that our specifically designed dual-site collaborative catalytic mechanism will open up a new way to offers new opportunities for advancing sustainable NH_(3)production.

PHOTOINDUCED S_(RN)1 REACTIONS OF ARYL HALIDES WITH CARBAZOLYL NITROGEN ANION

The photoinduced reactions of aryl halides with carbazolyl nitrogen anion,in dimethyl sulfoxide,yield the corresponding N-arylated products.These reactions are suggested in terms of the S;Nl mechaism of nucleophilic substitution.

外源H_(2)S对盐胁迫下红砂幼苗叶片和根系氮代谢的影响

H_(2)S作为新型气体信号分子在调控植物生长发育和抗逆境胁迫中发挥着重要作用。为探究外源H_(2)S对盐胁迫下红砂(Reaumuria soongorica)氮代谢的影响机制及最佳叶施浓度,以当年生红砂幼苗为材料,采用盆栽试验,以1/2 Hoagland浇灌为对照(CK)考察在300 mmol·L^(-1)NaCl胁迫(CK_(300))下,叶面喷施不同浓度(0、0.010、0.025、0.050、0.100、0.250、0.500、1 mmol·L^(-1))H_(2)S供体硫氢化钠(NaHS)对红砂叶片和根系中硝酸还原酶(NR)、谷氨酰胺合成酶(GS)、谷氨酸合成酶(GOGAT)活性以及硝态氮、可溶性蛋白和游离氨基酸含量的影响。结果表明,盐胁迫下,红砂幼苗根、叶中可溶性蛋白、游离氨基酸、硝态氮含量以及NR、GOGAT、GS活性均较对照(CK)显著下降。经不同浓度外源H_(2)S处理后,红砂根、叶中可溶性蛋白含量与单独盐胁迫对照(CK_(300))相比显著降低,NR、GOGAT、GS活性和硝态氮、游离氨基酸含量显著升高。综合分析认为,NR和GS可作为H_(2)S对盐胁迫下红砂幼苗缓解效应的主要评价指标,且外源H_(2)S对盐胁迫下红砂幼苗最适缓解浓度为0.025 mmol·L^(-1)。综上,外施适宜浓度的NaHS能有效缓解盐胁迫对红砂幼苗氮代谢功能的抑制作用。本研究结果为深入了解红砂耐盐机理和培育红砂优质种质资源提供了理论依据。

Simultaneous nitrification and autotrophic denitrification in fluidized bed reactors using pyrite and elemental sulfur as electron donors

In this study, simultaneous nitrification and autotrophic denitrification (SNAD) with either elemental sulfur or pyrite were investigated in fluidized bed reactors in mesophilic conditions. The reactor performance was evaluated at different ammonium (12-40 mg/L of NH4+-N), nitrate (35-45 mg/L of NO3--N), and dissolved oxygen (DO) (0.1-1.5 mg/L) concentrations, with a hydraulic retention time of 12 h. The pyrite reactor supported the SNAD process with a maximum nitrogen removal efficiency of 139.5 mg/(L·d) when the DO concentration was in the range of 0.8-1.5 mg/L. This range, however, limited the denitrification efficiency of the reactor, which decreased from 90.0% ± 5.3% in phases II-V to 67.9% ± 7.2% in phases VI and VII. Sulfate precipitated as iron sulfate (FeSO4/Fe2(SO4)3) and sodium sulfate (Na2SO4) minerals during the experiment. The sulfur reactor did not respond well to nitrification with a low and unstable ammonium removal efficiency, while denitrification occurred with a nitrate removal efficiency of 97.8%. In the pyrite system, the nitrifying bacterium Nitrosomonas sp. was present, and its relative abundance increased from 0.1% to 1.1%, while the autotrophic denitrifying genera Terrimonas, Ferruginibacter, and Denitratimonas dominated the community. Thiobacillus, Sulfurovum, and Trichlorobacter were the most abundant genera in the sulfur reactor during the entire experiment.

Modified Conway Method for soil available nitrogen testing

The ideal reducing reagent in testing available nitrogen of soil by Conway Methodis Devard‘s alloy.The optimum ratio of soil:Devard’s alloy:1.0N NaOH solution is 5.0g:0.2g:5ml.When reaction goes on at 30℃ for 4 hours,the rate of soil NO3-N reduction is about 90%,and the recovery rate of soil NH4-N is about 95%.Some organic nitrogen can be measured at thesame time.This method is defined as the Modified Conway Method and the soil N-min is r=0.982（n=10）.In predicting soil nitrogen supplying power,the Modified Conway Method takesonly 1/6 of time needed by Conway Method.The Modified Conway Method can also be used forthe measure of soil N-min.

N缺陷g-C_(3)N_(5)修饰S掺杂苝酰亚胺增强可见光自芬顿苯酚氧化耦合Cr(Ⅵ)还原

通过静电自组装制备有机复合半导体N缺陷g-C_(3)N_(5)(NVs)修饰S掺杂苝酰亚胺(S-PDI)。NVs具有丰富的活性位点,而具有氨基基团的酰胺增强了S-PDI与NVs的分子间作用力。NVs质量分数30%的30%NVs/S-PDI对Cr(Ⅵ)的还原率为79.96%,对苯酚的降解率为74.40%;30%NVs/S-PDI协同氧化苯酚与还原Cr(Ⅵ)过程中,Cr(Ⅵ)的还原率为92.83%,苯酚的降解率为93.89%,即苯酚的氧化降解促进了Cr(Ⅵ)的还原,Cr(Ⅵ)的还原增强了苯酚的氧化降解。NVs/S-PDI充分利用导带的还原性能和价带的氧化性能,实现电子空穴的空间分离,协同强化光催化过程中的氧化半反应和还原半反应,同步提升光催化氧化还原性能。同时,光照产生的电子、H_(2)O_(2)与Cr(Ⅵ)形成一个光自芬顿反应过程,进一步促进了苯酚的氧化降解与Cr(Ⅵ)的还原去除。

西藏驱龙巨型斑岩Cu-Mo矿床的富S、高氧化性含矿岩浆——来自岩浆成因硬石膏的证据

西藏冈底斯成矿带上的驱龙巨型斑岩Cu-Mo矿床以发育大量的硬石膏为特征。详细的岩相学研究发现:驱龙矿床不仅发育热液脉状的硬石膏,含矿斑岩中还产出岩浆成因硬石膏,这在国内系首次报道。观察表明,在岩浆演化早期的花岗闪长岩、黑云母二长花岗岩中,岩浆硬石膏以矿物包体的形式产于斜长石、石英中;在主成矿期的二长花岗斑岩、花岗闪长斑岩中,岩浆硬石膏以矿物包体产于斜长石斑晶中,局部以斑晶形式产出,并伴有富S的磷灰石(SO3含量为0.11%～0.44%)、磁铁矿发育。后期热液活动也形成了大量的硬石膏±石英+硫化物脉,是矿区主要的矿化类型之一。电子探针分析结果显示,后期的热液硬石膏与岩浆硬石膏相比,在微量成分上明显富集Sr(分别为0.24%和0.03%),可能是由于在岩浆-热液演化过程中,Sr的不相容性或者/以及粘土化蚀变造成的。岩浆硬石膏以及与之共生的富S磷灰石的出现,明确指示驱龙矿床成矿岩浆具有富S、高氧逸度的特征;同时也为研究S在斑岩型矿床岩浆演化过程中的状态、行为,提供了很好的研究对象。

红花黄色素联合尿激酶对急性脑梗死患者血清MMP-9、S-100B蛋白和NO的影响

目的探讨红花黄色素联合尿激酶治疗急性脑梗死的临床疗效及对患者血清基质金属蛋白酶-9(MMP-9)、S-100B蛋白和一氧化氮(NO)的影响。方法选取160例符合纳入标准的急性脑梗死患者作为研究对象,按照随机数字表法随机分为两组,每组各80例。常规治疗基础上,对照组患者给予尿激酶溶栓治疗,观察组在对照组基础上联合红花黄色素治疗。采用美国国立卫生研究院卒中量表(NIHSS)评价患者神经功能缺损情况。比较组间临床疗效、NIHSS评分、血清MMP-9、S-100B蛋白、NO水平。结果脑梗死临床疗效显示,观察组患者治疗总有效率高于对照组(91.2%vs.80.0%,P<0.05)。治疗后,与对照组相比,观察组患者NIHSS评分降低(13.1±3.6分vs.16.8±4.2分),血清MMP-9、S-100B蛋白、NO水平降低(72.6±11.4 ng/ml vs.90.1±12.5 ng/ml,122.3±35.6 ng/L vs.174.8±40.9 ng/L,65.4±7.3μmol/L vs.70.2±8.5μmol/L),组间比较均有统计学差异(P均<0.05)。结论红花黄色素联合尿激酶在急性脑梗死中治疗效果良好,能够明显提高临床疗效,改善神经功能缺损,并降低血清MMP-9、S-100B蛋白、NO水平,临床上值得进一步研究。