A series of small-molecular fluorescent probes based on nitrogen-fused pyridazinone scaffold were developed in this report.The design strategy involved two steps:1)enhancing the electron-withdrawing ability of the acc...A series of small-molecular fluorescent probes based on nitrogen-fused pyridazinone scaffold were developed in this report.The design strategy involved two steps:1)enhancing the electron-withdrawing ability of the acceptor by incorporating an N-heterocyclic aromatic ring(pyridine or pyrazine)at the C4 and C5 positions of the pyridazinone skeleton and 2)anchoring a triphenylphosphine or morpholine tail as the subcellular targeting group.These fluorescent probes displayed excellent properties in live cell and brain tissue imaging.展开更多
基金Beijing Natural Science Foundation(Grant No.7162110)Interdisciplinary Medicine Seed Fund of Peking University(Grant No.BMU2018MC004)the National Natural Science Foudation of China(Grant No.201807006,81872731,91630314)
文摘A series of small-molecular fluorescent probes based on nitrogen-fused pyridazinone scaffold were developed in this report.The design strategy involved two steps:1)enhancing the electron-withdrawing ability of the acceptor by incorporating an N-heterocyclic aromatic ring(pyridine or pyrazine)at the C4 and C5 positions of the pyridazinone skeleton and 2)anchoring a triphenylphosphine or morpholine tail as the subcellular targeting group.These fluorescent probes displayed excellent properties in live cell and brain tissue imaging.
