Synergistic effects of planting density and nitrogen fertilization on chlorophyll degradation and leaf senescence after silking in maize

Regulating planting density and nitrogen(N)fertilization could delay chlorophyll(Chl)degradation and leaf senescence in maize cultivars.This study measured changes in ear leaf green area(GLA_(ear)),Chl content,the activities of Chl a-degrading enzymes after silking,and the post-silking dry matter accumulation and grain yield under multiple planting densities and N fertilization rates.The dynamic change of GLA_(ear)after silking fitted to the logistic model,and the GLA_(ear) duration and the GLAearat 42 d after silking were affected mainly by the duration of the initial senescence period(T_(1))which was a key factor of the leaf senescence.The average chlorophyllase(CLH)activity was 8.3 times higher than pheophytinase activity and contributed most to the Chl content,indicating that CLH is a key enzyme for degrading Chl a in maize.Increasing density increased the CLH activity and decreased the Chl content,T1,GLAear,and GLA_(ear) duration.Under high density,appropriate N application reduced CLH activity,increased Chl content,prolonged T1,alleviated high-density-induced leaf senescence,and increased post-silking dry matter accumulation and grain yield.

Effects of Slow-release Nitrogen on Dry Matter Accumulation,Translocation and Yield of Summer Maize

[Objectives]This study was conducted to investigate the effects of slow-release nitrogen fertilizer on dry matter accumulation and translocation of summer maize.[Methods]With Zhoudan 9 as the test variety,six different treatment were set up:blank control(CK1),slow-release urea 75 kg/hm^(2)(C1),slow-release urea 150 kg/hm^(2)(C2),slow-release urea 225 kg/hm^(2)(C3),slow-release urea 300 kg/hm^(2)(C4)and ordinary urea 300 kg/hm^(2)(CK2),to study the change law of dry matter accumulation and translocation in summer maize.[Results]Treatment slow-release urea 225 kg/hm^(2)(C4)showed summer maize yield,dry matter translocation between organs,grain contribution rate and proportion of grain dry matter in the full ripe stage higher than other treatments.Considering the weight loss and cost factors,slow-release urea 225 kg/hm^(2)(C3)could be recommended as the fertilizing amount for summer maize.[Conclusions]This study provides theoretical reference for rational selection of fertilizers for reducing fertilizer application and increasing fertilizer efficiency,and for production of summer maize in Shajiang black soil region.

Effects of Slow-release Nitrogen Fertilizer on Yield and Nitrogen Accumulation of Summer Maize in Shajiang Black Soil Area

[Objectives] This study was conducted to verify the field application effect of slow-release nitrogen fertilizer on summer maize in Shajiang black soil area by simultaneous sowing and fertilization, and explore the application scope and nitrogen metabolism mechanism, so as to lay a foundation for fertilizer reduction and efficiency improvement. [Methods] With maize variety Beiqing 340 and sulfur-coated urea as experimental materials, five nitrogen application levels were set, namely, control (C0), slow-release nitrogen 70 kg/hm^(2) (C70), slow-release nitrogen 140 kg/hm^(2) (C140), slow-release nitrogen 210 kg/hm^(2) (C210) and slow-release nitrogen 280 kg/hm^(2) (C280). The phosphorus and potassium fertilizers were all in accordance with the unified standard. [Results] With the application rate of slow-release nitrogen increasing, the nitrogen accumulation in organs increased first and then decreased after tasseling stage of maize. In order to reduce the fertilizing amount and increase efficiency, 210 kg/hm^(2) of slow-release nitrogen fertilizer was the best fertilizing amount for summer maize in Shajiang black soil area. [Conclusions] This study provides reference for fertilizer reduction, efficiency improvement and sustainable development of summer maize in Shajiang black soil area.

Characteristics and Performance of Novel Water-Absorbent Slow Release Nitrogen Fertilizers

In this article, a research on the characteristics and performance of water-absorbent slow release nitrogen fertilizer （WASRNF） using infrared spectroscopy （IR）, scanning electronic microscopy （SEM）, differential scanning calorimetry （DSC）, and thermogravimetry analysis （TGA）, was present. The results indicate that the water absorbency and nitrogen analysis of WASRNF is 103 g g^-1 and 30%, respectively, and WASRNF exhibits approximately neutral pH and very low salt index. WASRNF is a copolymer of nitrogen fertilizer and super absorbent polymer （SAP） monomers which is formed through hydrogen bond interaction, and the molecule contains hydrophilic groups, which is responsible for the absorption and water retention capacity of the molecule. WASRNF is a gel that exhibits the ability to swell, but does not dissolve in water. WASRNF shows non-homogenous nature as a whole, but in local zone it is homogenous, the copolymer molecule shows chain network that is the physical structure responsible for absorption and retention of water in WASRNF. The water retained in WASRNF exists as free and nonfreezing bound and freezing bound water states, with the free and the nonfreezing water accounting for more than 95% of water retained in WASRNF, and the nonfreezing bound water for less than 5%. WASRNF functions in delaying the release of nitrogen from it, thereby serving a novel slow release nitrogenous fertilizer.

Effect of Nitrogenous Fertilizer Treatment on Mineral Metabolism in Grazing Yaks

To assess the impact of N fertilization on contents of mineral elements in herbage and the effect of increased forage S on the copper metabolism of grazing yaks, study was conducted during the summer grazing season （2005, 2006, and 2007）. Pasture replicates （20 ha; n=3 per treatment） received the same fertilizer treatment in each growing season, consisting of i） 90 kg N ha^-1 from quickly available nitrogen, ii） 90 kg N ha^-1 from ammonium nitrate, iii） 90 kg N ha^-1 from ammonium sulfate, and iv） control （no fertilizer）. Forage sampling was collected at 60 days intervals following fertilization （10 samples per pasture） for Cu, Mo, Mn, Se, Fe, Zn, Ca, and P. To determine the effect of fertilizer treatment on mineral metabolism in grazing yaks, liver and blood samples were collected at the start and end of the study period in 2005, 2006, and 2007. Ammonium sulfate fertilization increased （P 〈 0.01） forage S concentration. Plant tissue N concentrations were increased by N fertilization, regardless of source in 2005, 2006, and 2007. Yaks grazing S fertilization pastures had lower （P〈0.05） liver and blood Cu concentrations at the end of the study period in 2005, 2006, and 2007, compared with urea, ammonium nitrate, and control. Nominal increases in forage in vitro organic matter digestibility were realized by fertilization, regardless of N source in each year.

Nitrate leaching of winter wheat grown in lysimeters as affected by fertilizers and irrigation on the North China Plain

Proper application of nitrogen（N） fertilizers and irrigation management are important production practices that can reduce nitrate leaching into groundwater and improve the N use efficiency（NUE）. A lysimeter/rain shelter facility was used to study effects of the rate of N fertilization, type of N fertilizer, and irrigation level on key aspects of winter wheat production over three growing seasons（response variables were nitrate transport, N leaching, and NUE）. Results indicated that nitrate concentration in the soil profile and N leaching increased with the rate of N fertilization. At the end of the third season, nitrate concentration in the top 0–75 cm layer of soil was higher with manure treatment while urea treatments resulted in higher concentrations in the 100–200 cm layer. With normal irrigation, 3.4 to 15.3% of N from applied fertilizer was leached from the soil, yet no leaching occurred under a stress irrigation treatment. The manure treatment experienced less N leaching than the urea treatment in all cases except for the 180 kg N ha^-1 rate in 2011–2012（season 3）. In terms of grain yield（GY）, dry matter（DM） or NUE parameters, values for the manure treatment were lower than for the urea treatment in 2009–2010（season 1）, yet were otherwise higher for urea treatment in season 3. GY and crop nitrogen uptake（NU） were elevated when the rate of N fertilizer increased, while the NUE decreased; GY, DM, and NU increased with the amount of irrigation. Data indicated that reduced rates of N fertilization combined with increased manure application and proper irrigation management can lower nitrate levels in the subsoil and reduce potential N leaching into groundwater.

Impact of atrazine and nitrogen fertilizers on the sorption of chlorotoluron in soil and model sorbents

Sorption of chlorotoluron in ammonium sulfate, urea and atrazine multi-solutes system was investigated by batch experiments. The results showed application of nitrogen fertilizers to the soil could affect the behavior of chlorotoluron. At the same concentration of N, sorption of chlorotoluron decreased as the concentration of atrazine increased on the day 0 and 6 in soil, respectively. The sorption of chlorotoluron increased from 0 to 6 d when soils were preincubated with deionized water, ammonium sulfate and urea solution for 6 d. That indicated incubation time was one of the most important factors for the sorption of chlorotoluron in nitrogen fertilizers treatments. The individual sorption isotherms of chlorotoluron in rubbery polymer and silica were strictly linear in single solute system, but there were competition sorption between pesticides or between pesticides and nitrogen fertilizers. That indicated the sorption taken place by concurrent solid-phase dissolution mechanism and sorption on the interface of water-organic matter or water-mineral matter.

Denitrification Losses and N_(2)O Emissions from Different Nitrogen Fertilizers Applied to the Maize-Fluvo-Aquic Soil System

A field experiment was conducted to investigate the variations in denitrification losses and N2O emissions from 4 different types of nitrogen fertilizers (urea, ammonium nitrate, ammonium bicarbonate, and calcium nitrate) applied to the maize- fluvo-aquic soil system in the North China Plain by the method of intact soil core incubation and acetylene inhibition, and the responses of nitrogen fertilizers to maize grain yields. Results show that the denitrification loss from different nitrogen fertilizers ranged from 0.38-1.20 kg N ha-1, with no significant differences among different fertilizer treatments, and the N2O emission from 0.05-0.95 kg N ha-1, with a significant difference (P<0.05) among the treatments. The highest emission was from the treatment of ammonium nitrate, while the lowest from calcium nitrate. The nitrogen fertilizers increased the maize grain yield by 9.7-19.8% compared to control. But there were no significant differences in yield increase among the 4 types of nitrogen fertilizers. In comparison, urea had the best effect, whereas calcium nitrate had the least effect on increasing maize yield. The maize yield was 5.7% higher when urea was separately applied at 2 times than when it was applied at a time. In this case, however, the denitrification loss and the N2O emission were also increased by 4.05 and 1.84 kg N ha-1, respectively.

Effects of Nitrogenous Fertilizer on Pungency of Hot Pepper (Capsicum annuum L.) Fruits

The response of pungency of hot pepper fruits nitrogenous fertilizer on was invesigated. The results indicated that nitrogenous fertilizer had a significant effect on the capsaicin content of hot pepper fruits at 35 and 42 days after flowering;, capsaicin content gradually decreased, while peroxidase activity increased with nitrogenous fertilizer increasing.

Effects of nitrogen fertilizer on protein synthesis, accumulation, and physicochemical properties in common buckwheat

Nitrogen(N)fertilization affects grain quality in common buckwheat(Fagopyrum esculentum Moench).But the effects of N fertilizer on various buckwheat protein parameters are not fully understood.This study aimed to investigate the synthesis,accumulation,and quality of buckwheat protein under four N application rates in the Loess Plateau,China.Optimal N application(180 kg N ha-1)improved yield,agronomic traits,and N transport and increased protein yield and protein component accumulation.Prolamin and glutelin accumulation first increased and then decreased with increasing N application.The relationships between the contents of protein components and the amount of applied N generally followed quadratic functions.Nitrate reductase and glutamine synthetase activities first increased and then decreased with increasing N levels.Optimal N fertilizer increased the waterholding capacity and thermal stability of buckwheat protein and reduced its emulsification capacity,but negligibly changed its oil-absorption capacity.Hydrophobic amino acids and glutelin content were the main factors affecting protein quality.

Effects of tropical forest conversion into oil palm plantations on nitrous oxide emissions:A meta-analysis

Oil palm plantations have dramatically expanded in tropical Asia over the past decades.Although their establishment has been projected to increase nitrous oxide(N_(2)O)emissions,earlier reports have shown inconsistent results.This study analyzed these previously published data to compare N_(2)O emissions in oil palm plantations to reference forests.A linear mixed-eff ects model was used to examine the signifi cance of the eff ect of establishing oil palm plantations on N_(2)O emissions,rather than to calculate mean eff ect sizes because of limitations in the data structure.The results indicated that N_(2)O emissions were signifi cantly greater from oil palm plantations than from reference forests,as expected.This is the fi rst study to report the eff ect of oil palm plantations on N_(2)O emissions by synthesizing previously published data.To quantify the size of this eff ect,additional studies with frequent and long-term monitoring data are needed.

Yield of Roselle (Hibiscus sabdariffa L.) as Influenced by Manure and Nitrogen Fertilizer Application

Field experiments were carried out on roselle (Hibiscus sabdariffa L) during the 2019 and 2020 cropping seasons at the CSIR-SARI Research Station at Manga in the Upper East Region of Ghana. The aim of the experiment was to study the response of roselle to cowdung, nitrogen fertilizer and their interaction on the growth and yield of roselle. The treatments consisted of factorial combination of five rates of cowdung (0, 1.5, 2.5, 3.5 and 4.5 t/ha) and five levels of nitrogen (0, 20, 40, 60 and 80 kgN/ha) laid out in a randomized complete block design and replicated three times. Data were collected on days to 50% flowering, plant height, number of leaves per plant and dry calyx yield. The results indicated that differences in dry calyx yield due to the manure and nitrogen fertilizer effects were highly significant (P 0.01). Application rate of 2.5 t/ha manure recorded the highest yield of 340 kg/ha and 308.1 kg/ha in 2019 and 2020 respectively. Increasing manure rate from 2.5 - 3.5 t/ha did not result in significant increases in dry calyx yield. The four rates differed significantly from the control which had the least calyx yield of 190.3 kg/ha and 180 kg/ha in 2019 and 2020 respectively. Nitrogen rate of 60 kg/ha recorded the highest dry calyx yield of 510.5 kg/ha and 370.4 kg/ha in 2019 and 2020 respectively which were significantly different from calyx yields recorded by other treatments. The trend on partial budget analysis was consistent in both seasons with the highest yielding treatments (2.5 t/ha cowdung manure and 60 kg/ha) recording the highest net benefit while the control (0 kg/ha) ranked last. The application rate of 2.5 t/ha of cowdung and 60 kg/ha of Nitrogen is thus recommended for optimum roselle production and productivity in the study area.

Effects of Combined Application of Biochar-based Organic Fertilizer and Reduced Nitrogen Fertilizer on Soil Enzyme Activity and Yield of Purple Cabbage(Brassica oleracea var.capita rubra)in Yuanmou County

[Objectives]In response to the issue of soil improvement in Yuanmou County,the effects of combined application of biochar-based organic fertilizer and reduced nitrogen fertilizer on soil nutrients,soil enzyme activity,and yield of purple cabbage(Brassica oleracea var.capita rubra)were investigated in the field base of Institute of Thermal Zone Ecological Agriculture,Yunnan Academy of Agricultural Sciences in Yuanmou County.[Methods]A total of 13 treatments were set up by applying biochar-based organic fertilizer at three levels of 15,30 and 45 t/hm^(2)(T_(1),T_(2),T_(3)),combined with top application of nitrogen fertilizer(urea)at four levels:375(N_1),300(N_(2)),225(N_(3))and 0 kg/hm^(2),with non-fertilizing treatment as control check(CK),in order to explore the optimal ratio for the combined application of biochar-based organic fertilizer with nitrogen fertilizer.[Results]The application of biochar-based organic fertilizer could significantly improve soil nutrients,enzyme activity,and purple cabbage yield.The improvement effect of combined application with nitrogen fertilizer was higher than that of single application of biochar-based organic fertilizer,and the improvement effect was enhanced with the application amount of biochar-based organic fertilizer increasing.The contents of organic matter and total nitrogen were the highest in treatment T_(3)N_(3),of which the values increased by 81.39%and 56.09%compared with the CK,respectively.The contents of soil hydrolyzable nitrogen,available phosphorus,and available potassium were all the highest under treatment T_(3)N_(2),with increases of 92.76%,171.01%and 235.50%,respectively.There was a significant positive correlation between the activity of soil catalase,urease,and sucrase and organic matter,total nitrogen,and available nutrients.The overall soil enzyme activity was relatively higher in treatment T_(3)N_(2).The yield of purple cabbage treated with biochar-based organic fertilizer combined with nitrogen fertilizer could reach 85750 kg/hm^(2),which was 94.78%higher than that treated with biochar-based organic fertilizer alone.Based on comprehensive analysis,the optimal combination ratio was 45 t/hm^(2)of biochar-based organic fertilizer and 300 kg/hm^(2)of urea(T_(3)N_(2)).[Conclusions]This study provides data support for the promotion of biochar-based organic fertilizers and reduced fertilizer in agricultural soil in the Dam area of Yuanmou County.

Effects of Different Nitrogen and Phosphorus Synergistic Fertilizer on Enzymes and Genes Related to Nitrogen Metabolism in Wheat

In recent years,in order to improve nutrient use efficiency,especially nitrogen use efficiency,fertilizer valueadded technology has been developed rapidly.However,the mechanism of the effect of synergistic fertilizer on plant nitrogen utilization is not clear.A study was,therefore,conducted to explore the activities and gene expression of key enzymes for nitrogen assimilation and the gene expression of nitrogen transporters in wheat after the application of synergistic fertilizer.Soil column experiment was set up in Qingdao Agricultural University experimental base from October 2018 to June 2019.Maleic acid and itaconic acid were copolymerized with acrylic acid as cross-linking monomer to make a fluid gel,which was sprayed on the fertilizer surface to make nitrogen and phosphorus synergistic fertilizer.A total of 6 treatments was set according to different nitrogen and phosphorus fertilizer ratios:(1)100%common nitrogen fertilizer+100%common phosphate fertilizer(2)70%nitrogen synergistic fertilizer+100%phosphorus synergistic fertilizer;(3)100%nitrogen synergistic fertilizer+70%phosphorus synergistic fertilizer;(4)100%nitrogen synergistic fertilizer+100%phosphorus synergistic fertilizer;(5)70%nitrogen synergistic fertilizer+70%phosphorus synergistic fertilizer;(6)100%commercial nitrogen synergistic fertilizer+100%commercial phosphorus synergistic fertilizer.The results are as follows:(1)the enzyme activities of wheat plants under synergistic fertilizer condition were higher than those under ordinary fertilizer,except under the treatment that nitrogen and phosphorus synergistic fertilizer were both reduced;(2)the expression level of the genes under the treatment“100%nitrogen synergistic fertilizer+100%phosphorus synergistic fertilizer”was significantly higher than those in other treatments.Combined with the higher performance of nitrogen concentration in various parts of the plant under the condition of applying synergistic fertilizer,this study indicated that the application of synergistic fertilizer can improve the nitrogen metabolism of the plant by increasing the nitrogen level in the rhizosphere soil,inducing the expression of nitrogen transporter genes and key assimilation enzymes genes.

Effects of Different Proportions of Controlled Release Urea and Ordinary Urea on Peanut Yield

[Objectives]To study the effects of different proportions of controlled release urea and ordinary urea on peanut yield.[Methods]A total of 5 treatments were set up according to different proportions of controlled release urea and ordinary urea,randomly arranged in blocks and repeated 3 times.[Results]The test results of field districts showed that different proportions of controlled release urea and conventional urea had different effects on peanut yield.On the basis of applying 50 kg/666.7 m^(2)of calcium superphosphate and 17 kg/666.7 m^(2)of potassium sulfate,13.34 kg/666.7 m^(2)of pure nitrogen was applied.The optimal ratio of controlled release urea to ordinary urea was 75:25,followed by 50:50.The output was 379.83 and 371.83 kg/666.7 m^(2),separately increased by 6.74%and 4.50%compared to the application of ordinary urea.[Conclusions]The combined application of controlled release urea and ordinary urea in peanuts can significantly increase peanut yield compared to just applying ordinary urea.

Optimum Application Rate of Nitrogen in Summer Peanut in Southern Shandong Area

[Objectives]To study the effect of nitrogen(N)on the growth demand of summer peanuts under a certain level of phosphorus and potassium fertilizers,and to carry out experiments on nitrogen fertilizer control of summer peanuts.[Methods]Four treatments were set up in the experiment:no-nitrogen plot(N 0P_(4)K_(4)),optimized nitrogen plot(N_(7)P_(4)K_(4)),70%optimized nitrogen plot(N_(5)P_(4)K_(4)),130%optimized nitrogen plot(N 9P 4K 4),repeated 3 times,and arranged in random blocks.The area of the plot was 42 m^(2),ridges were set between the plots,and protective rows of more than 1 m were set around the experimental site.The types of fertilizers were urea,superphosphate,and imported potassium chloride,and the variety of peanuts was Linhua 5.Except for the level of fertilization,other agricultural operations were the same,and soil sampling tests,field records,and yield testing were carried out according to the requirements of the plan.[Results]On the basis of 60 kg/ha of phosphorus and potassium fertilizer application,the optimum economical fertilizer application rate and the highest application rate of pure nitrogen were about 115.20 and 131.25 kg/ha,respectively.[Conclusions]This study is expected to provide a certain basis for the high-quality and high-yield summer peanuts in southern Shandong area.

Inhibition of nitrification in soil by metal diethyldithiocarbamates

Nitrification acts as a key process in determining fertilizer use efficiency by crops as well as nitrogen losses from soils. Metal dithiocarbamates in addition to their pesticidal properties can also inhibit biological oxidation of ammonium(nitrification) in soil. Metal [M=V(Ⅲ), Cr(Ⅲ), Mn(Ⅱ), Fe(Ⅲ), Ni(Ⅱ), Cu(Ⅱ), Zn(Ⅱ) and Co(Ⅱ)] diethyldithiocarbamates (DEDTC) were synthesized by the reaction of sodium diethyldithiocarbamate with metal chloride in dichloromethane/water mixture. These metal diethyldithiocarbamates were screened for their ability to inhibit nitrification at different concentrations(10 μg/g soil, 50 μg/g soil and 100 μg/g soil). With increasing concentration of the complex, capacity to retard nitrification increased but the extent of increase varied for different metals. At 100 μg/g soil, different complexes showed nitrification inhibition from 22 36% to 46 45%. Among the diethyldithiocarbamates tested, Zn(DEDTC) 2 proved to be the most effective nitrification inhibitor at 100 μg/g soil. Manganese, iron and chromium diethyldithiocarbamates also proved to be effective nitrification inhibitors than the others at 100 μg/g soil. The order of percent nitrification inhibition in soil by metal diethyldithiocarbamates was: Zn(Ⅱ) > Mn(Ⅱ) > Fe(Ⅲ) > Cr(Ⅲ) >V(Ⅲ) > Co(Ⅱ) > Ni(Ⅱ) > Cu(Ⅱ).

Effects of Nitrogen Application on Chlorophyll Fluorescence Parameters and Leaf Gas Exchange in Naked Oat

Naked oat（Avena nuda L.） was originated from China,where soil nitrogen（N） is low availability.The responses of chlorophyll（Chl.） fluorescence parameters and leaf gas exchange to N application were analysed in this study.After the N application rate ranged from 60 to 120 kg ha-1,variable fluorescence（F v）,the maximal fluorescence（F m）,the maximal photochemical efficiency（F v /F m）,quantum yield（Φ PS II） of the photosynthetic system II（PS II）,electron transport rate（ETR）,and photochemical quenching coefficient（qP） increased with N application level,however,non-photochemical quenching coefficient（qN） decreased.Moreover,there was no difference in initial fluorescence（F o） with further more N enhancement.The maximum net photosynthetic rate（P max）,apparent dark respiration rate（R d） and light saturation point（LSP） were improved with 40-56 kg N ha-1as basal fertilizer and 24-40 kg N ha-1as top dressing fertilizer applied at jointing stage.Initial quantum yield（α） was decreased with 24 kg N ha-1as basal fertilizer and 56 kg N ha-1as top dressing fertilizer.Flag-leaf net photosynthetic rate（P n） was significantly enhanced at the jointing and heading stages with 40-56 kg N ha-1as basal fertilizer; in addition,increased at grain filling stage of naked oat with 40-56 kg N ha-1as top dressing fertilizer.90 kg N ha-1（50-70% as basal fertilizer and 30-50% as top dressing fertilizer） application is recommended to alleviate photodamage of photosystem and improve the photosynthetic rate in naked oat.

Effects of Different Nitrogen Fertilizer Levels on Fe,Mn,Cu and Zn Concentrations in Shoot and Grain Quality in Rice (Oryza sativa)

In a pot experiment, effects of N fertilizer application on the concentrations of Fe, Mn, Cu and Zn in shoot of rice and the quality of brown rice were studied. In the treatments with N fertilizer application, the concentrations of Fe, Mn, Cu and Zn in most parts of rice shoot increased compared with control （no N fertilizer application）. This indicated that the transportation ability of microelements from root to shoot in rice was improved with N fertilizer application. Effect of N fertilizer on IR68144 was similar to that of on IR64, but the concentrations of the microelements in plant differed, suggesting that the characteristic expression of the two rice genotypes was not controlled by the amount of N fertilizer supplied. The concentrations of those microelements in brown rice increased at first and then decreased with increasing N fertilizer application, reaching the highest at 160 kg/ha, at which the Fe, Mn, Cu and Zn concentrations in brown rice increased by 28.96%, 41.34%, 58.31% and 16.0% for IR64, and by 22.16%, 13.75%, 8.75% and 20.21% for IR68144 compared with control, respectively. Moreover, N fertilizer promoted the accumulation of protein, decreased the accumulation of amylose in grain, and enhanced gel consistency of brown rice. These results indicate that appropriate N fertilizer management could increase micronutrient contents in grain and improve nutrition quality of rice.

Effects of Nitrogen Application Rate and Ratio on Lodging Resistance of Super Rice with Different Genotypes

The objective of this study was to determine the morphology mechanism of nitrogen （N） fertilizer rates and ratio on lodging resistance through analying its effects among lodging index （LI）, lodging-related morphological traits and physical strength in basal intemodes by comparing japonica and indica super rice cultivars. Field experiments, with three nitrogen levels （0, 150 and 300 kg ha-L） and two ratios of basal to topdressing （8：2 and 5：5） with two super rice cultivars （Yliangyou 2 and Wuyunjing 23）, were conducted in the Baolin Farm, Danyang Country, Jiangsu Province, China, in 2011 and 2012. Effects of N fertilizer rates and ratios on morphology of whole plant, morphology traits in basal intemodes and culm＇s physical strength parameters were investigated at 20 d after full heading stage. LI of Yliangyou 2 was significant greater than that of Wuyunjing 23 due to larger bending moment by whole plant （WP） with higher plant height and gravity center height. With higher volume of N fertilizer, LI of two super rice cultivars was increased conspicuously. However, no significant effect was detected with increase of panicle fertilizer ratio. The size of breaking strength （M） in basal intemodes was the key factor determining LI among N fertilizer treatments. Correlation analysis revealed that M value was positively related bending stress （BS） of Wuyunjing 23 and section modulus （Z） of Yliangyou 2, respectively. The higher N fertilizer levels induced reduction ofBS of Wuyunjing 23 due to weak culm and leaf sheath plumpness status and reduced Z of Yliangyou 2 owning to small diameter and culm wall thickness, consequently, influencing their M indirectly. These results suggested that breaking strength was the key factor influencing LI with increase of N fertilizer levels. However, the lodging-related morphology mechanism was different with genotypes. Culm wall thickness and diameter in basal internodes of indica super rice and culm and leaf sheath plumpness status of japonica super rice influenced breaking strength, as well as lodging index, respectively.