Piggy Bac is a transposable DNA element originally discovered in the cabbage looper moth(Trichoplusia ni).The T.ni piggyBac transposon can introduce exogenous fragments into a genome,constructing a transgenic organism...Piggy Bac is a transposable DNA element originally discovered in the cabbage looper moth(Trichoplusia ni).The T.ni piggyBac transposon can introduce exogenous fragments into a genome,constructing a transgenic organism.Nevertheless,the comprehensive analysis of endogenous piggyBac-like elements(PLEs)is important before using piggyBac,because they may influence the genetic stability of transgenic lines.Herein,we conducted a genome-wide analysis of PLEs in the brown planthopper(BPH)Nilaparvata lugens(St?l)(Hemiptera:Delphacidae),and identified a total of 28 PLE sequences.All N.lugens piggy Bac-like elements(NlPLEs)were present as multiple copies in the genome of BPH.Among the identified NlPLEs,NlPLE25 had the highest copy number and it was distributed on five chromosomes.The full length of NlPLE25 consisted of terminal inverted repeats and sub-terminal inverted repeats at both terminals,as well as a single open reading frame transposase encoding546 amino acids.Furthermore,NlPLE25 transposase caused precise excision and transposition in cultured insect cells and also restored the original TTAA target sequence after excision.A cross-recognition between the NlPLE25 transposon and the piggy Bac transposon was also revealed in this study.These findings provide useful information for the construction of transgenic insect lines.展开更多
基金the Foundation of Guangzhou Science and Technology Key Project(No.201904020041),China。
文摘Piggy Bac is a transposable DNA element originally discovered in the cabbage looper moth(Trichoplusia ni).The T.ni piggyBac transposon can introduce exogenous fragments into a genome,constructing a transgenic organism.Nevertheless,the comprehensive analysis of endogenous piggyBac-like elements(PLEs)is important before using piggyBac,because they may influence the genetic stability of transgenic lines.Herein,we conducted a genome-wide analysis of PLEs in the brown planthopper(BPH)Nilaparvata lugens(St?l)(Hemiptera:Delphacidae),and identified a total of 28 PLE sequences.All N.lugens piggy Bac-like elements(NlPLEs)were present as multiple copies in the genome of BPH.Among the identified NlPLEs,NlPLE25 had the highest copy number and it was distributed on five chromosomes.The full length of NlPLE25 consisted of terminal inverted repeats and sub-terminal inverted repeats at both terminals,as well as a single open reading frame transposase encoding546 amino acids.Furthermore,NlPLE25 transposase caused precise excision and transposition in cultured insect cells and also restored the original TTAA target sequence after excision.A cross-recognition between the NlPLE25 transposon and the piggy Bac transposon was also revealed in this study.These findings provide useful information for the construction of transgenic insect lines.
