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长链非编码H19靶向调节miR-107通过Notch通路对肺癌的侵袭迁移的影响 被引量:3
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作者 李纪远 张灿斌 +3 位作者 马新 王强 王献 郑帅玉 《中国免疫学杂志》 CAS CSCD 北大核心 2017年第9期1392-1397,共6页
目的:探讨长链非编码H19靶向调节miR-107通过Notch通路对肺癌的侵袭迁移的影响情况。方法:q PCR检测肺癌和癌旁组织、不同肺癌细胞中H19的表达情况;Transwell侵袭实验检测沉默H19后肺癌细胞侵袭能力的变化;划痕实验检测沉默H19后肺癌细... 目的:探讨长链非编码H19靶向调节miR-107通过Notch通路对肺癌的侵袭迁移的影响情况。方法:q PCR检测肺癌和癌旁组织、不同肺癌细胞中H19的表达情况;Transwell侵袭实验检测沉默H19后肺癌细胞侵袭能力的变化;划痕实验检测沉默H19后肺癌细胞迁移能力的变化;双荧光素酶报告基因检测H19与miR-107的相互作用;q PCR检测肺癌和癌旁组织、不同肺癌细胞中miR-107的表达情况;Transwell侵袭实验检测沉默H19后miR-107对肺癌细胞侵袭能力的影响;划痕实验检测沉默H19后miR-107肺癌细胞迁移能力的影响;裸鼠皮下成瘤检测沉默H19后miR-107对肺癌成瘤大小以及体积的影响。Western blot检测沉默H19后Notch通路蛋白的表达情况。结果:与癌旁组织相比,肺癌组织中H19表达明显增高;肺癌细胞A549中H19表达水平最高;沉默H19可以抑制肺癌细胞侵袭和迁移能力;H19能与miR-107的3'UTR特异性结合;与癌旁组织相比,肺癌组织中miR-107表达明显降低;沉默H19后,抑制miR-107可以促进肺癌细胞侵袭和迁移能力;与H19-siRNA组相比,H19-siRNA+miR-107-inhibitor组荷瘤小鼠肿瘤体积和重量都明显增大。沉默H19后Notch通路蛋白表达情况相应下调。结论:H19在肺癌发生发展过程中起重要作用,H19可以靶向调节miR-107通过Notch信号通路调控肺癌细胞的侵袭和迁移能力。 展开更多
关键词 H19 肺癌 miR-107 侵袭试验
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Studies on adaptability of Populus bolleana and other Poplar trees in northwest Shanxi Province 被引量:2
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作者 吕皎 《Journal of Forestry Research》 SCIE CAS CSCD 2001年第1期31-34,共5页
Through five years (1996-2000) experiment, the growth characteristics and resistance to insect and cold of the seven poplar varieties including P. po pularis, P. opera, P. pseudo-simonii, P. beijinensis, P. bolleana,... Through five years (1996-2000) experiment, the growth characteristics and resistance to insect and cold of the seven poplar varieties including P. po pularis, P. opera, P. pseudo-simonii, P. beijinensis, P. bolleana, P. ( eurameri cana and P. simonii, which were planted in loess hilly land in northwest Shanxi Province, were tested under different planting densities and site conditions. A randomized block design and three times repetition were adopted in the test. Bas ed on the test results in arid and cold area of the northwest Shanxi, the hybrid poplar trees taking Populus cathaysna as their female parent could be selected for afforestation, such as P. popularis and P. opera, and the growing space of s ingle plant should be larger than 20 m2. P. bolleana as an introduced tree speci es grows well in this area, but its growth is likely affected by ground water co ndition. On the site with relatively abundant soil water, P. bolleana usually su ffer from frost crack at its trunk base. As a result, P. bolleara is suited to p lanting in the site where is short of soil water. P. x euramericana is not suita ble for large-scale afforestation, especially in arid slopes and ridges of loess hilly land . 展开更多
关键词 poplar trees Arid and cold area ADAPTABILITY Shanxi Province
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血清微小RNA-107、白细胞介素-18与慢性肾功能衰竭患者肾移植术后急性肾损伤的关系 被引量:1
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作者 王华 刘海平 +3 位作者 白玲 祝锦江 张永鹏 陈国振 《中国临床医生杂志》 2024年第7期818-822,共5页
目的探究血清微小RNA-107(miR-107)、白细胞介素-18(IL-18)水平对慢性肾功能衰竭患者肾移植术后急性肾损伤(AKI)的关系及其评估价值。方法收集2022年10月至2023年10月在西安交通大学第一附属医院接受肾移植手术的120例慢性肾功能衰竭患... 目的探究血清微小RNA-107(miR-107)、白细胞介素-18(IL-18)水平对慢性肾功能衰竭患者肾移植术后急性肾损伤(AKI)的关系及其评估价值。方法收集2022年10月至2023年10月在西安交通大学第一附属医院接受肾移植手术的120例慢性肾功能衰竭患者为研究对象。根据术后1周内是否并发AKI分为AKI组(46例)与非AKI组(74例)。比较两组患者血清miR-107、IL-18及基线资料,采用logistic回归性分析检验血清miR-107、IL-18与并发AKI的关系;绘制受试者工作特征(ROC)曲线,以曲线下面积(AUC)分析血清miR-107、IL-18对肾移植患者后并发AKI的评估价值。结果AKI组患者血清miR-107、IL-18、血肌酐(Scr)、血尿素氮(BUN)水平明显高于非AKI组患者,肾小球滤过率低于非AKI组,差异有统计学意义(P<0.05)。多因素logistic回归分析显示,血清miR-107、IL-18、Scr是影响肾移植患者术后并发AKI的危险因素(P<0.05)。ROC曲线结果显示,血清miR-107、IL-18均具有评估并发AKI的效能,并且当二者串联时其效能明显提升。结论血清miR-107、IL-18水平升高与肾移植术后并发AKI有关系,且二者对并发AKI均具有评估价值,串联联合应用时可提升评估效能。 展开更多
关键词 微小RNA-107 白细胞介素-18 慢性肾功能衰竭 肾移植 急性肾损伤
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PDCD4和miR-107在阴茎癌组织中的表达及与患者预后的关系
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作者 王志 何长海 +9 位作者 董彪 邱学德 李保安 赵琪 王鸿 朱海冬 刘俊峰 刘正道 吕明珠 江专新 《现代肿瘤医学》 CAS 2024年第14期2620-2624,共5页
目的:探究程序性细胞死亡因子4(PDCD4)及微小RNA-107(miR-107)在阴茎癌组织中的表达水平及与患者预后的关系。方法:选取2018年01月至2020年06月经本院确诊阴茎癌并行手术治疗患者32例,术中分别取患者阴茎癌组织(研究组)及癌旁正常阴茎组... 目的:探究程序性细胞死亡因子4(PDCD4)及微小RNA-107(miR-107)在阴茎癌组织中的表达水平及与患者预后的关系。方法:选取2018年01月至2020年06月经本院确诊阴茎癌并行手术治疗患者32例,术中分别取患者阴茎癌组织(研究组)及癌旁正常阴茎组织(对照组)各32对,应用实时定量PCR(quantitative reverse transcription-PCR)检测两组PDCD4和miR-107的表达水平并分析其与临床病理参数及预后的关系,并采用Kaplan-Meier进行生存曲线分析及Logistic回归分析影响阴茎癌患者预后的因素。结果:研究组PDCD4表达量明显低于对照组,miR-107表达量明显高于对照组(P均<0.05);PDCD4和miR-107的表达与阴茎癌患者淋巴结转移、组织分级相关(P均<0.05),与年龄、吸烟史、饮酒史、肿瘤部位无关(P均>0.05);应用Kaplan-Meier法对生存率进行分析,miR-107低表达组生存率显著高于miR-107高表达组,PDCD4低表达组生存率低于PDCD4高表达组(P均<0.05)。多因素分析结果显示,TNM分期高、淋巴结转移、PDCD4低表达和miR-107高表达均是影响阴茎癌患者预后的独立危险因素(均P<0.05)。结论:阴茎癌患者miR-107呈异常高表达,PDCD4呈低表达,二者均是影响阴茎癌的独立危险因素且有望作为预测阴茎癌患者预后的重要指标。 展开更多
关键词 程序性细胞死亡因子4 阴茎癌 微小RNA-107 预后
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miR-107靶向NID2通过Notch信号通路调控肺癌细胞的增殖侵袭 被引量:8
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作者 吴瑕 何君 王文军 《中国免疫学杂志》 CAS CSCD 北大核心 2017年第11期1662-1667,共6页
目的:miR-107靶向NID2调控Notch信号通路影响肺癌的侵袭和增殖。方法:免疫组化检测NID2在肺癌组织和正常肺组织中的表达;PCR检测miR-107在肺癌组织中的表达;双荧光素酶报告基因系统检测miR-107对NID2转录活性的影响;肿瘤细胞成球实验检... 目的:miR-107靶向NID2调控Notch信号通路影响肺癌的侵袭和增殖。方法:免疫组化检测NID2在肺癌组织和正常肺组织中的表达;PCR检测miR-107在肺癌组织中的表达;双荧光素酶报告基因系统检测miR-107对NID2转录活性的影响;肿瘤细胞成球实验检测miR-107的表达对肺癌细胞A549的增殖能力的影响;Transwell侵袭实验检测miR-107的表达对肺癌A549细胞的侵袭能力的影响;划痕试验检测miR-107的表达对肺癌A549细胞的迁移能力的影响;Western blot检测过表达miR-107后Notch信号通路的蛋白表达水平。结果:和正常肺组织比较,NID2在肺癌组织中表达较高;miR-107在肺癌组织中表达明显降低;双荧光素酶报告基因系统检测结果显示,miR-107可以直接调控NID2的转录活性;过表达miR-107后,肺癌细胞A549的增殖、侵袭和迁移能力明显降低;过表达miR-107后,Notch1、hes-1、presenilin1蛋白表达下调。结论:miR-107靶向NID2的表达,通过Notch通路调控肺癌细胞的增殖和侵袭能力。 展开更多
关键词 miR-107 肺癌 noTCH A549 侵袭 迁移
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miR-107对口腔鳞癌细胞系CAL27增殖、侵袭及迁移的影响
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作者 张晋玮 王燕 王通 《天津医药》 CAS 2024年第9期897-900,共4页
目的探讨miR-107在口腔鳞癌细胞系CAL27中的表达及其对CAL27细胞增殖、凋亡、侵袭和迁移的影响。方法采用实时荧光定量PCR检测miR-107在口腔鳞癌细胞株CAL27和人口腔上皮细胞HOEC细胞中的表达情况;脂质体转染法将miR-107 mimic和miR-107... 目的探讨miR-107在口腔鳞癌细胞系CAL27中的表达及其对CAL27细胞增殖、凋亡、侵袭和迁移的影响。方法采用实时荧光定量PCR检测miR-107在口腔鳞癌细胞株CAL27和人口腔上皮细胞HOEC细胞中的表达情况;脂质体转染法将miR-107 mimic和miR-107 NC质粒分别转染进入CAL27细胞,并分为对照(miR-107 NC)组和miR-107过表达(miR-107 mimic)组;MTT细胞增殖实验检测细胞增殖能力,流式细胞术检测细胞凋亡率,Transwell小室实验检测细胞迁移和侵袭能力。结果CAL27细胞系中miR-107的表达低于HOEC细胞。miR-107 mimic组细胞转染后24、48、72 h光密度(OD)值均低于miR-107 NC组,凋亡细胞比例高于miR-107 NC组,而侵袭和迁移细胞数少于miR-107 NC组(P<0.05)。结论上调miR-107可抑制CAL27细胞的增殖、侵袭和迁移,并促进其凋亡。 展开更多
关键词 口腔肿瘤 RNA干扰疗法 细胞增殖 肿瘤浸润 细胞运动 miR-107
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超声C-TIRADS联合血清miR-107检测对甲状腺结节性质的诊断价值
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作者 张媛 鲍应军 +3 位作者 葛妍 武秀兰 张连花 张芬 《中国实验诊断学》 2024年第9期1030-1035,共6页
目的探讨超声甲状腺影像报告和数据系统(C-TIRADS)联合血清微小RNA-107(miR-107)检测对甲状腺结节性质的诊断价值。方法选取2022年4月至2024年1月新疆医科大学附属肿瘤医院患甲状腺结节的患者98例(共113个结节)作为研究对象,均进行C-TIR... 目的探讨超声甲状腺影像报告和数据系统(C-TIRADS)联合血清微小RNA-107(miR-107)检测对甲状腺结节性质的诊断价值。方法选取2022年4月至2024年1月新疆医科大学附属肿瘤医院患甲状腺结节的患者98例(共113个结节)作为研究对象,均进行C-TIRADS分类,并检测血清miR-107水平。根据手术病理是否出现恶性结节分恶性组、良性组。分析超声C-TIRADS分类、血清miR-107水平与甲状腺结节良恶性的关系及其对甲状腺结节的诊断价值,并进行校准曲线分析。结果98例甲状腺结节患者(113个结节)中有83个良性结节,包括结节性甲状腺肿49个、甲状腺腺瘤24个、桥本甲状腺炎10个,其余30个恶性结节均为甲状腺乳头状癌。恶性结节均位于包膜下,结节内部钙化,C-TIRADS分类为4b或4c。恶性组患者血清miR-107水平高于良性组(P<0.05)。行Logistic多因素回归分析结果显示结节位置位于包膜下、结节内部钙化、C-TIRADS分类为4b或4c、miR-107是恶性甲状腺结节的独立危险因素(P<0.05)。超声C-TIRADS联合血清miR-107水平诊断甲状腺结节性质的曲线下面积(AUC)值为0.907,大于二者单独诊断甲状腺结节性质的AUC值(0.702和0.835)(Z=3.300,P=0.001;Z=2.410,P=0.016)。超声C-TIRADS联合血清miR-107水平诊断甲状腺结节性质的预测值与真实值一致性较好,Brier得分为0.114,校准斜率为0.809,校准度良好。结论超声C-TIRADS联合血清miR-107水平在诊断甲状腺结节性质方面具有较高临床应用价值。 展开更多
关键词 超声甲状腺影像报告和数据系统 微小RNA-107 甲状腺结节 血清
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Variation of soil enzyme activity and microbial biomass in poplar plantations of different genotypes and stem spacings 被引量:6
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作者 Ye Li Liping Zhang +2 位作者 Shengzuo Fang Ye Tian Jiao Guo 《Journal of Forestry Research》 SCIE CAS CSCD 2018年第4期958-967,共10页
To improve the productivity of poplar planta- tions, a field experiment of split-plot design with four tree spacings and three poplar clones was established, and four soil enzyme activities and microbial biomass were ... To improve the productivity of poplar planta- tions, a field experiment of split-plot design with four tree spacings and three poplar clones was established, and four soil enzyme activities and microbial biomass were monitored in the trial. Soil enzyme activities, in most cases, were significantly higher in topsoil (0-10 cm) than in lower horizons (10-20 cm). Soil cellulase, catalase and protease activities during the growing season were higher than during the non-growing season, while invertase activity followed the opposite trend. Soil invertase, cellulase and catalase activities varied by poplar clone but soil protease activity did not. Cellulase and protease activities in the plantation at 5 × 5 m spacing were significantly higher than in the other spacings. The highest catalase activity was recorded at 6 × 6 m spacing. At the same planting density, invertase activity was greater in square spacings than in rectangular spacings. Soil microbial biomass was also significantly affected by seedling spacing and poplar clone. The mean soil MBC was significantly lower in topsoil than in the lower horizon, while MBN showed the opposite pattern. Significantly positive correlations were observed among soil cellulase, protease and catalase activities (p 〈0.01), whereas soil invertase activity was negatively and significantly correlated with cellulase, protease and catalase activities (p 〈 0.01). Soil microbial biomass and enzyme activities were not correlated except for a significantly negative correlation between soil MBC and catalase activities. Variations in soil enzyme activity and microbial biomass in different poplar plantations suggest that genotype and planting spacing should be considered when modeling soil nutrient dynamics and managing for long-term site productivity. 展开更多
关键词 Microbial activity Nutrient availability Planting density poplar clone Seasonal variation
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MiR-107靶向Notch2促进胃癌细胞迁移和侵袭 被引量:5
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作者 余南荣 曾祥 +1 位作者 徐厚巍 蓝俊松 《第三军医大学学报》 CAS CSCD 北大核心 2019年第12期1142-1148,共7页
目的检测微小RNA-107(miR-107)在胃癌细胞中的表达,探讨其调控胃癌细胞迁移和侵袭的分子机制。方法采用Real-time PCR检测miR-107和Notch2在人胃癌细胞MKN45和人胃黏膜上皮细胞GES-1中的表达。转染miR-107 inhibitors至MKN45细胞,采用Tr... 目的检测微小RNA-107(miR-107)在胃癌细胞中的表达,探讨其调控胃癌细胞迁移和侵袭的分子机制。方法采用Real-time PCR检测miR-107和Notch2在人胃癌细胞MKN45和人胃黏膜上皮细胞GES-1中的表达。转染miR-107 inhibitors至MKN45细胞,采用Transwell法检测MKN45细胞迁移和侵袭。转染Notch2过表达载体至MKN45细胞,采用Transwell、Real-time PCR、Weatern blot分别检测细胞迁移、侵袭和基质金属蛋白酶-9(matrix metalloproteinase-9,MMP-9)的表达。Targetscan在线预测和双荧光素酶报告基因实验验证miR-107和Notch2的靶向关系。Transwell实验检测抑制miR-107并沉默Notch2后MKN45细胞迁移和侵袭。结果与GES-1细胞比较,MKN45细胞中miR-107表达上调(P<0.01),而Notch2表达下调(P<0.01)。抑制miR-107或过表达Notch2,则MKN45细胞迁移和侵袭数减少(P<0.01),过表达Notch2能促进MMP-9 mRNA和蛋白的表达(P<0.01)。Targetscan在线预测和双荧光素酶报告基因实验结果表明:Notch2是miR-107的潜在靶基因,抑制miR-107可促进Notch2表达(P<0.01)。在MKN45细胞中沉默Notch2,可逆转miR-107抑制剂对细胞迁移和侵袭的抑制作用。结论 miR-107在胃癌细胞中表达上调,可通过靶向Notch2促进MMP-9表达,促进胃癌细胞迁移和侵袭。 展开更多
关键词 miR-107 noTCH2 胃癌 细胞迁移 肿瘤侵袭
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Differences of EST-SSR and genomic-SSR markers in assessing genetic diversity in poplar 被引量:4
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作者 SONG Yue-peng JIANG Xi-bing +5 位作者 ZHANG Man WANG Ze-liang BO Wen-hao AN Xin-min Zhang De-qiang ZHANG Zhi-yi 《Forestry Studies in China》 CAS 2012年第1期1-7,共7页
We analyzed the genetic differences of 16 poplar clones between genomic-SSR and EST-SSR markers. The statistical results show that the average number of alleles detected by genomic-SSR was 4.1, Shannon's index 1.0646... We analyzed the genetic differences of 16 poplar clones between genomic-SSR and EST-SSR markers. The statistical results show that the average number of alleles detected by genomic-SSR was 4.1, Shannon's index 1.0646, observed heterozygos- ity 0.4427 and expected heterozygosity 0.5523, while for the EST-SSR, the average number of alleles was 2.8, Shannon's index 0.6985, observed heterozygosity 0.2330 and expected heterozygosity 0.4684. Cluster analysis indicated that the EST-SSR capacity of genotypic identification was more precise than that of genomic-SSR. These resuks reveal that EST-SSR and genomic-SSR have statistically significant genetic differences in polymorphism detection and genotypic identification. These differences could provide a theoretical basis for the rational use of SSR markers in species diversity and other related research. 展开更多
关键词 poplar GEnoMIC-SSR EST-SSR genetic differences
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Use of amino silane coupling agent to improve physical and mechanical properties of UF-bonded wheat straw(Triticum aestivum L.) poplar wood particleboard 被引量:3
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作者 Seyedeh Masoumeh Hafezi Aliakbar Enayati +2 位作者 Kazem Doost Hosseini Asghar Tarmian Sayed Ahmad Mirshokraii 《Journal of Forestry Research》 SCIE CAS CSCD 2016年第2期427-431,共5页
We evaluated the potential use of amino silane coupling agent (SiNH) to improve physical and mechanical properties of UF-bonded wheat straw (Triticurn aestivum L.) poplar wood particleboard. We examined the effect... We evaluated the potential use of amino silane coupling agent (SiNH) to improve physical and mechanical properties of UF-bonded wheat straw (Triticurn aestivum L.) poplar wood particleboard. We examined the effects of varied content of silane coupling agent content and ratios of straw to poplar wood particles on particleboard prop- erties. The ratios of straw to poplar wood particles were 100:0, 85:15, 70:30 and 55:45. Silane coupling agent content was tested at three levels, 0, 5 and 10 %. The experimental panels were tested for their mechanical strength, including modulus of elasticity (MOE), modulus of rupture (MOR), intemal bonding (IB) and physical properties according to procedures specified in DIN 68763 (Chipboard for special purposes in building construction: concepts, requirements, testing, 1982-03, 1982). All board properties were improved by the addition of silane cou- pling agent. The use of poplar wood particles had a positive effect on the mechanical properties of wheat straw parti- cleboard but had a negative effect on physical properties (thickness swelling and water absorption). 展开更多
关键词 Wheat straw particleboard poplar wood UFresin Silane coupling age
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Dynamic changes of phenolic compound contents in leaf and bark of poplar during autumn temperature drop 被引量:2
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作者 LI Chun-ming WANG Yang YU Wen-xi 《Journal of Forestry Research》 SCIE CAS CSCD 2011年第3期481-485,共5页
We investigated seasonal dynamics of phenolics substance in leaf and bark of two cultivars of poplar, Populus alba×berolinensis and P. בZhonglin Sanbei 1’, during autumn temperature drop for analyzing the... We investigated seasonal dynamics of phenolics substance in leaf and bark of two cultivars of poplar, Populus alba×berolinensis and P. בZhonglin Sanbei 1’, during autumn temperature drop for analyzing the roles of phenolic secondary metabolites in cold resistance. Results show that the contents of condensed tannin and flavonoid in poplar leaf and the flavonoid contents in bark of P. בZhonglin Sanbei 1’ were increased with the decrease of autumn minimum temperature, showing a significantly negative correlation between the contents of soluble phenolic substance in the leaves and changeable temperature. In contrast, lignin content in the poplar leaves is decreased in the process of temperature drop, showing a significantly positive correlation. These results indicate that the variation in phenolic substance has a close correlation with its cold-resistance during the autumn temperature drop. 展开更多
关键词 poplar natural temperature drop condensed tannin flavonoid LIGNIN
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Growth variations and stability analyses of seven poplar clones at three sites in northeast China 被引量:3
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作者 Xiaona Pei Luping Jiang +4 位作者 Ammar khalil Mohamed Ahmed Hongying Yu Rizheng Chong Xiangling You Xiyang Zhao 《Journal of Forestry Research》 SCIE CAS CSCD 2021年第4期1673-1680,共8页
Growth characteristics have complex inheritance patterns and genotype(G) by environment(E) interaction make predicting tree response to environmental changes difficult.In this study,the growth of seven poplar clones a... Growth characteristics have complex inheritance patterns and genotype(G) by environment(E) interaction make predicting tree response to environmental changes difficult.In this study,the growth of seven poplar clones at three different sites was taken as the research focus,and heights and basal diameters were investigated in the second growing season.An ANOVA showed that all main effects,site,clone number and their interactions were highly significant in the overall F-tests.The coefficients of variation and repeatability of different traits ranged from 15.5 to 43.9%and from 0.549 to 0.912,respectively.AMMI(Additive Main Effects and Multiplicative Interaction) analysis results showed that genotype,environment and G × E interaction were significantly highly correlated.The stability analysis indicated that different clones showed different growth traits on different sites,which suggests that elite clones should be selected separately for different sites.Based on the growth traits,under a 10% selection rate,three clones were selected for different sites and the genetic gains of growth traits ranged from 4.7 to 11.2%.The three selected clones could be used to establish plantations in the future in different sites. 展开更多
关键词 poplar Hybrid clone GEnoTYPE Environmental interaction Stability REPEATABILITY
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Effect of Steam Explosion Technology Main Parameters on Moso Bamboo and Poplar Fiber 被引量:2
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作者 Biqing Shu Qin Ren +7 位作者 Lu Hong Zhongping Xiao Xiaoning Lu Wenya Wang Junbao Yu Naiqiang Fu Yiming Gu Jinjun Zheng 《Journal of Renewable Materials》 SCIE EI 2021年第3期585-597,共13页
One of the large-scale industrial applications of Moso bamboo and poplar in China is the production of standardized fiberboard.When making fiberboard,a steam blasting pretreatment without the addition of traditional a... One of the large-scale industrial applications of Moso bamboo and poplar in China is the production of standardized fiberboard.When making fiberboard,a steam blasting pretreatment without the addition of traditional adhesives has become increasingly popular because of its environmental friendliness and wide applicability.In this study,the steam explosion pretreatment of Moso bamboo and poplar was conducted.The steam explosion pressure and holding time were varied to determine the influence of these factors on fiber quality by investigating the morphology of the fiber,the mass ratio of the unexploded specimen at the end face,the chemical composition,and the tensile strength.The following conclusions were drawn:As the steam burst pressure and holding time increased,more cellulose and hemicellulose degradation occurred(the degradation of hemicellulose was greater than that of cellulose),the lignin content rose,and the fiber bundle strength decreased.The degradation of bamboo cellulose was slightly higher than that of poplar,and the degradation of poplar hemicellulose was significantly faster than that of bamboo.Furthermore,increasing the steam explosion pressure and pressure holding time could not effectively increase the lignin content.It is recommended to use a steam blasting pressure of 2.5 MPa or 3.0 MPa and a holding time of 180 s to perform steam blasting on bamboo and poplar specimens. 展开更多
关键词 FIBER binderless fiberboard steam explosion moso bamboo poplar
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Identification of CD13,CD107a,and CD164 as novel basophil-activation markers and dissection of two response patterns in time kinetics of IgE-de-pendent upregulation 被引量:10
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作者 Florian HENNERSDORF Stefan FLORIAN +6 位作者 Andreas JAKOB Katharina BAUMGÄRTNER Karoline SONNECK Alfred NORDHEIM Tilo BIEDERMANN Peter VALENT Hans-Jörg BüHRING 《Cell Research》 SCIE CAS CSCD 2005年第5期325-335,共11页
Using two-colour flow cytometry>200 antibodies submitted to the 8^(th) International Workshop of Human Leukocyte Differentiation Antigens(HLDA8)have been analyzed for their reactivity with resting and activated CD2... Using two-colour flow cytometry>200 antibodies submitted to the 8^(th) International Workshop of Human Leukocyte Differentiation Antigens(HLDA8)have been analyzed for their reactivity with resting and activated CD203c^(+)basophils.Four antibodies either non-reactive or weakly reactive with resting basophils exhibited an increased reactivity with basophils activated by anti-IgE-mediated cross-linking of the high affinity IgE receptor(FcεRI).These include antibod-ies against CD164(WS-80160,clone N6B6 and WS-80162,clone 67D2),as well as two reagents with previously unknown specificities that were identified as CD13(WS-80274,clone A8)and CD107a(WS-80280,clone E63-880).The activation patterns followed either the“CD203c-like”or“CD63-like”activation profile.The CD203c profile is characterized by a rapid and significant upregulation(of CD13,CD164,and CD203c),reaching maximum levels after 5-15 min of stimulation.The phosphoinositide-3-kinase(PI3K)-specific inhibitor wortmannin inhibited the upregulation of these markers whereas 12-O-tetradecanoyl-phorbol-13-acetate(TPA)induced a rapid and FcεRI-independent upregulation within 1-2 min.In the CD63 profile,maximum upregulation(of CD63 and CD107a)was detected only after 20-40 min,and upregulation by TPA reached maximum levels after 60 min.In summary,our data identify CD13,CD107a,and CD164 as novel basophil-activation antigens.Based on time kinetics of upregulation,we hypothesize that molecules of the“CD203c group”and the“CD63 group”are linked to two different mechanisms of basophil activation. 展开更多
关键词 CD203c CD164 CD63 CD107a basophil activation basophil marker.
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Effects of densification on untreated and nano-aluminum-oxide impregnated poplar wood 被引量:3
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作者 Hamid R.Taghiyari Ghonche Rassam Kazem Ahmadi-DavazdahEmam 《Journal of Forestry Research》 SCIE CAS CSCD 2017年第2期403-410,共8页
Effects of densification of poplar wood(Populus nigra) impregnated with nano-aluminum oxide(NA) and pre-treated with water vapor for 4 and 6 h were investigated in the present study. Physical and mechanical proper... Effects of densification of poplar wood(Populus nigra) impregnated with nano-aluminum oxide(NA) and pre-treated with water vapor for 4 and 6 h were investigated in the present study. Physical and mechanical properties of treated poplar wood were measured according to the ASTM D-143 standard specifications, and then compared with the untreated specimens. Results showed significant improvement in all properties as a result of densification. A 4-h vapor pre-treatment improved effects on both physical and mechanical properties. When the duration of vapor-treatment increased to 6 h, wood polymers degraded to the extent that the improvements due to the vapor pre-treatment decreased substantially, though the final results were still significant improvements compared with the control specimens. High thermal conductivity coefficient of NA slightly but not significantly improved properties. Due to the high spring-back after 15 days,densified poplar is not recommended for applications in which densified wood will be exposed for long periods to high humidity or to direct water. 展开更多
关键词 poplar untreated nigra degraded specifications exposed pretreatment modulus Heidelberg swelling
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转抗虫基因107杨对根际和内源细菌群落的影响
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作者 路文然 任亚超 +3 位作者 赵津 王进茂 郭甫鑫 张军 《西部林业科学》 CAS 北大核心 2024年第1期72-81,共10页
为研究转抗虫基因对根际和内源细菌群落的影响,以转抗虫基因107杨田间对比试验林为对象,采用高通量测序技术对转基因株系和对照株系根际土壤细菌、树干韧皮部和根系内生细菌群落结构组成及多样性进行对比分析。结果显示:(1)土壤样品中... 为研究转抗虫基因对根际和内源细菌群落的影响,以转抗虫基因107杨田间对比试验林为对象,采用高通量测序技术对转基因株系和对照株系根际土壤细菌、树干韧皮部和根系内生细菌群落结构组成及多样性进行对比分析。结果显示:(1)土壤样品中有效序列平均在30000以上,杨树组织有效序列平均在300000以上。(2)转基因株系细菌物种组成与对照株系的极其相似,酸杆菌门与变形菌门为转基因和非转基因株系根际土壤的优势菌门,占比分别为16.6%和21.9%;蓝藻菌门和变形菌门为转基因和非转基因株系韧皮部的优势菌门,所占的比率分别为54.8%和59.2%;变形菌门为转基因和非转基因株系根部的优势菌门,所占的比率分别为40.7%和58.8%。(3)转基因和非转基因株系在细菌群落的丰富度和多样性上均没有表现出明显的差异,但不同部位之间差异显著。结果表明:转基因107杨没有对根际土壤和内生细菌造成显著影响,明确了转抗虫基因107杨不同部位细菌群落特征,为转抗虫基因107杨的生物安全评价提供了有益的参考。 展开更多
关键词 转抗虫基因107 高通量测序 细菌群落 丰富度 多样性
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A STUDY ON THE PHYSICAL MECHANISM OF RESISTANCE OF MAIN POPLAR STRAINS AGAINST CRYPTORRHYNCHUS LAPATHIL.IN NORTH CHINA 被引量:1
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作者 Liu Jingquan Hou Aiju Ding Fujun Northeast Forestry University 《Journal of Northeast Forestry University》 SCIE CAS CSCD 1993年第1期91-106,共16页
The physical mechanism of resistance of poplar against Cryptorrhynchus lapathiL.is directly correlated with the morphological structure of bark,concerning the following 5aspects:1.It is correlated with whether surface... The physical mechanism of resistance of poplar against Cryptorrhynchus lapathiL.is directly correlated with the morphological structure of bark,concerning the following 5aspects:1.It is correlated with whether surface bark of larva transvered tunnel crack or not,ear-ly or late.Those that do not crack or crack late are susceptible strains while those that crack ear-ly are resistant strains.2.It is correlated with thickness of cork layer.Thick cork layer can in-crease resistance against pest while thin one is more susceptible to pest attack.3.It is related withage of tree.Trees at the age of 1-2 possess immunity to the pest,at 3-4 are relatively resistant,at5-8 are susceptible and over 9 are more resistant.4.It is also correlated with the rate of trees‘healing round the holes of larva tunnels.Those healing faster are resistant while those healingslower arc susceptible.5.It is related with the number of various scars on bark surface. 展开更多
关键词 poplar SUSCEPTIBLE TUNNELS crease faster concerning morphological cracking LARVAE immunity
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Comparative Study on the Properties of Inorganic Silicate and Organic Phenolic Prepolymer Modified Poplar Wood by Vacuum Cycle Pressurization 被引量:2
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作者 Pengfei Guan Ping Li +3 位作者 Yiqiang Wu Xingong Li Guangming Yuan Yingfeng Zuo 《Journal of Renewable Materials》 SCIE EI 2022年第9期2451-2463,共13页
To enhance mechanical properties and improve flame retardancy and smoke suppression of fast-growing poplar wood in wood applications,the wood was impregnated and modified.An organic phenolic prepolymer and inorganic s... To enhance mechanical properties and improve flame retardancy and smoke suppression of fast-growing poplar wood in wood applications,the wood was impregnated and modified.An organic phenolic prepolymer and inorganic sodium silicate was used as contrasting impregnation modifiers and wood samples were impregnated by a bionic“respiration”method with alternating positive and negative pressure.The weight percentage gain,density increase ratio,mechanical properties(bending and compressive strength and hardness),and water absorption rate of inorganic and organic-impregnated modified poplar wood(IIMPW and OIMPW,respectively)were compared and these properties in IIMPW were found to be higher than those of OIMPW with the exception of the water absorption rate which was lower than the OIMPW.This was attributed to the superior absorption of sodium silicate that also improved the impregnation,reinforcement,and dimensional stability in the IIMPW.The chemical structure,crystalline structure,internal morphology,flame retardancy,smoke suppression,and thermal stability of IIMPW and OIMPW were characterized by FT-IR,XRD,SEM,CONE,and TGA.FT-IR and XRD results showed that,although IIMPW cellulose crystallinity reduced the most,more chemical bonds were come into being in IIMPW,which explained the better physical and mechanical properties of IIMPW.Compared with OIMPW,IIMPW had better flame retardant and smoke suppression performance. 展开更多
关键词 poplar wood sodium silicate impregnation modification mechanical properties dimensional stability flame retardancy and smoke suppression
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Selecting the Technology of Sodium Silicate Modified Poplar with the Highest Performance by Fuzzy Orthogonal Method 被引量:1
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作者 Xiaoqian Bi Pengfei Guan +3 位作者 Ping Li Yuan Zhang Xingong Li Yingfeng Zuo 《Journal of Renewable Materials》 SCIE EI 2023年第5期2399-2415,共17页
Sodium silicate modification can improve the overall performance of wood.The modification process has a great influence on the properties of modified wood.In this study,a new method was introduced to analyze the wood ... Sodium silicate modification can improve the overall performance of wood.The modification process has a great influence on the properties of modified wood.In this study,a new method was introduced to analyze the wood modification process,and the properties of modified wood were studied.Poplar wood was modified with sodium silicate by vacuum-pressure impregnation.After screening using single-factor experiments,an orthogonal experiment was carried out with solution concentration,impregnation time,impregnation pressure,and the cycle times as experimental factors.The modified poplar with the best properties was selected by fuzzy mathematics and characterized by SEM,FT-IR,XRD and TG.The results showed that some lignin and hemicellulose were removed from the wood due to the alkaline action of sodium silicate,and the orderly crystal area of poplar became disorderly,resulting in the reduction of crystallinity of the modified poplar wood.FT-IR analysis showed that sodium silicate was hydrolyzed to form polysilicic acid in wood,and structural analysis revealed the formation of Si-O-Si and Si-O-C,indicating that sodium silicate reacted with fibers on the wood cell wall.TG-DTG curves showed that the final residual mass of modified poplar wood increased from 25%to 67%,and the temperature of the maximum loss rate decreased from 343℃ to 276℃.The heat release and smoke release of modified poplar wood decreased obviously.This kind of material with high strength and fire resistance can be used in the outdoor building and indoor furniture. 展开更多
关键词 poplar wood sodium silicate impregnation modification fuzzy orthogonal method process optimization flame retardant
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