The investigation of the painting materials (pigments, binders and varnish) of Agarbiciu wooden church painted walls was done by employing both nondestructive (XRF and reflection FTIR spectroscopy for pigments) and de...The investigation of the painting materials (pigments, binders and varnish) of Agarbiciu wooden church painted walls was done by employing both nondestructive (XRF and reflection FTIR spectroscopy for pigments) and destructive methods (FTIR spectroscopy and GC-MS spectrometry for pigments and binders). The digital restoration of the interior of the wooden church was done using the physical-chemical composition of the painting layer, a metadata set that describes the timeline, detailed photographic documentation and the 3D scanned interior of the church.展开更多
Collagen from tendon of Yezo sika deer was prepared by limited pepsin digestion. The yield of collagen was very high;35.7% on the basis of lyophilized dry weight. The secondary structure of this collagen was different...Collagen from tendon of Yezo sika deer was prepared by limited pepsin digestion. The yield of collagen was very high;35.7% on the basis of lyophilized dry weight. The secondary structure of this collagen was different from that of porcine skin by ATR-FTIR analysis, although it was the same characteristics, e.g. SDS-PAGE, subunit composition, ther-mal behavior, as porcine collagen. Since taking up a problem of bovine spongiform encephalopathy infection in land animals such as calf or bovine, collagen from aquatic materials has been used in various industries. However, the present study indicates that tendon of Yezo sika deer as by-product of meat industry will have potential as an important collagen source for use in the foods, cosmetics, and medical fields.展开更多
Acid-soluble collagen was extracted from the skins of surf smelt and characterized. The yield of collagen was high about 24.0% on a dry weight basis. By SDS-polyacrylamide gel electrophoresis and CM-Toyopearl 650 M co...Acid-soluble collagen was extracted from the skins of surf smelt and characterized. The yield of collagen was high about 24.0% on a dry weight basis. By SDS-polyacrylamide gel electrophoresis and CM-Toyopearl 650 M column chromatography, this collagen is a heterotrimer with a chain composition of α1α2α3. The denaturation temperature was 32.5℃, about 4.5℃ lower than that from porcine skin. Attenuated total reflectance-fourier transform infrared analysis showed that the percentage of secondary structural components in this collagen were 11% α-helix, 34% β-sheet, 19% β-turn, and 21% others. It suggests that the triple helical structure is present in the acid-soluble collagen from the skins of the surf smelt in comparison to that from the skin of porcine.展开更多
文摘The investigation of the painting materials (pigments, binders and varnish) of Agarbiciu wooden church painted walls was done by employing both nondestructive (XRF and reflection FTIR spectroscopy for pigments) and destructive methods (FTIR spectroscopy and GC-MS spectrometry for pigments and binders). The digital restoration of the interior of the wooden church was done using the physical-chemical composition of the painting layer, a metadata set that describes the timeline, detailed photographic documentation and the 3D scanned interior of the church.
文摘Collagen from tendon of Yezo sika deer was prepared by limited pepsin digestion. The yield of collagen was very high;35.7% on the basis of lyophilized dry weight. The secondary structure of this collagen was different from that of porcine skin by ATR-FTIR analysis, although it was the same characteristics, e.g. SDS-PAGE, subunit composition, ther-mal behavior, as porcine collagen. Since taking up a problem of bovine spongiform encephalopathy infection in land animals such as calf or bovine, collagen from aquatic materials has been used in various industries. However, the present study indicates that tendon of Yezo sika deer as by-product of meat industry will have potential as an important collagen source for use in the foods, cosmetics, and medical fields.
文摘Acid-soluble collagen was extracted from the skins of surf smelt and characterized. The yield of collagen was high about 24.0% on a dry weight basis. By SDS-polyacrylamide gel electrophoresis and CM-Toyopearl 650 M column chromatography, this collagen is a heterotrimer with a chain composition of α1α2α3. The denaturation temperature was 32.5℃, about 4.5℃ lower than that from porcine skin. Attenuated total reflectance-fourier transform infrared analysis showed that the percentage of secondary structural components in this collagen were 11% α-helix, 34% β-sheet, 19% β-turn, and 21% others. It suggests that the triple helical structure is present in the acid-soluble collagen from the skins of the surf smelt in comparison to that from the skin of porcine.
