Endometrial development is a complicated process involving numerous regulatory factors.Circular RNAs(circRNAs)have been known as a member of the naturally occurring non-coding RNA family,and are reportedly crucial for...Endometrial development is a complicated process involving numerous regulatory factors.Circular RNAs(circRNAs)have been known as a member of the naturally occurring non-coding RNA family,and are reportedly crucial for a variety of physiological processes.This study investigated the circRNA landscape of non-pregnant endometrium of dairy goats during estrus.Non-pregnant endometrial samples of goats at estrus day 5(Ed5)and estrus day 15(Ed15)were used to methodically analyze the circRNA landscape using strand-specific Ribo-Zero RNA-Seq.A total of 2331 differentially expressed(P<0.05)circRNAs(DEciRs)between Ed5 and Ed15 were discovered in the goat endometrium.It was found that Nipped-B-like(NIPBL)and calcium responsive transcription factor(CARF)may participate in the development of the endometrium by decreasing(P<0.05)the levels of their circRNA-transcript forms.Furthermore,Gene Ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)analyses of DEciR host genes(hgDEciRs)revealed that tight junctions and GTPases may be involved in endometrial development during the estrus cycle.A total of 2331 DEciRs were discovered in the endometrium at Ed5 and Ed15.Based on GO and KEGG enrichment analyses,it could be inferred that tight junctions and GTPases are likely to play an important role in the development of goat endometrium during the estrus cycle.This circRNA study greatly enhances our knowledge of global trends in the development of non-pregnant endometrium during the estrus cycle in goats;these results help us to better understand the molecular regulation of endometrial development in dairy goats.展开更多
Background: Malaria infection during pregnancy is a major public health problem in tropical and subtropical regions globally. Anaemia is often an adverse outcome of severe parasitic infections during pregnancy in deve...Background: Malaria infection during pregnancy is a major public health problem in tropical and subtropical regions globally. Anaemia is often an adverse outcome of severe parasitic infections during pregnancy in developing countries. Pregnant women in malaria-endemic communities are more susceptible to Plasmodium falciparum infections than non-pregnant women of child-bearing age. Objective: To comparatively investigate malaria and anaemia in pregnant and non-pregnant women of child-bearing age. Design: A cross-sectional comparative study. Three hundred and eighty pregnant women and 380 non-pregnant women were screened for the study. Setting: The study was conducted at the University Hospital, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana. Measurements: Participants’ demographic data were collected via the administration of questionnaires. In addition their blood samples were analyzed for haemoglobin level and malaria parasites, while stool samples from the pregnant women were examined for intestinal parasites. Results: The study revealed that pregnant women have higher malaria parasitaemia (12.6%) and anaemia (62.6%). The species of Plasmodium isolated from the pregnant women were P. falciparum (85.4%), P. malariae (4.2%) and P. ovale (10.4%). Malaria parasitaemia was higher in the primigravidae (14%). However multigravidae recorded the highest anaemia prevalence (67.1%). Age of pregnant women was a factor affecting malaria parasitaemia with a significant P-value and OR (P value = 0.0041, 0R = 7.61). Conclusions: Pregnant women were more susceptible to malaria and anaemia than non-pregnant women of child-bearing age. Most of the pregnant women reported at antenatal clinic during the second trimester. Primigravidae however recorded the highest malaria parasitaemia. The main species of Plasmodium observed in the blood samples was falciparum.展开更多
目的了解孕妇对未来子女非免疫规划疫苗(expanded program on immunization,EPI)的接种意愿及其影响因素,为提高儿童非EPI接种率提供参考。方法采用便利抽样的方法,选取山西省妇幼保健院438名产检孕妇为研究对象进行问卷调查。利用独立...目的了解孕妇对未来子女非免疫规划疫苗(expanded program on immunization,EPI)的接种意愿及其影响因素,为提高儿童非EPI接种率提供参考。方法采用便利抽样的方法,选取山西省妇幼保健院438名产检孕妇为研究对象进行问卷调查。利用独立样本t检验、方差分析和多重线性回归模型分析个人社会经济因素、疫苗倾向和疫苗信息获取渠道对孕妇为未来子女接种非EPI意愿的影响,并基于计划行为理论构建结构方程模型分析孕妇对子女非EPI接种的态度、主观规范、知觉行为控制对接种意愿的影响。结果98.4%调查对象愿意为子女接种非EPI。与倾向于接种进口疫苗的群体相比,有接种国产疫苗倾向的群体(β=0.119)为子女接种非EPI的意愿更强,有接触媒体宣传较没有接触的群体子女非EPI接种意愿更强(β=0.099),有接触社区医院和疾病预防控制中心的宣传册和活动讲座的群体较没有接触的群体子女非EPI接种意愿更强(β=0.119)。结构方程模型结果显示影响接种意愿强度的因素从大到小依次为知觉行为控制、行为态度、主观规范(路径系数分别为0.73,0.13,0.12)。结论孕妇对未来子女非EPI的接种意愿水平较高。加强非EPI接种宣传教育,改变孕妇对非EPI的知觉行为控制和态度,能够帮助提高儿童非EPI接种率。展开更多
基金the China Postdoctoral Science Foundation(2019M653776,2020M673516)the National Key Research and Development Program of China(2016YFD0500508)+1 种基金the Natural Science Foundation of Shaanxi Province,China(2020JQ-265)the PhD research startup foundation of Northwest A&F University,China(00400/Z109021811)。
文摘Endometrial development is a complicated process involving numerous regulatory factors.Circular RNAs(circRNAs)have been known as a member of the naturally occurring non-coding RNA family,and are reportedly crucial for a variety of physiological processes.This study investigated the circRNA landscape of non-pregnant endometrium of dairy goats during estrus.Non-pregnant endometrial samples of goats at estrus day 5(Ed5)and estrus day 15(Ed15)were used to methodically analyze the circRNA landscape using strand-specific Ribo-Zero RNA-Seq.A total of 2331 differentially expressed(P<0.05)circRNAs(DEciRs)between Ed5 and Ed15 were discovered in the goat endometrium.It was found that Nipped-B-like(NIPBL)and calcium responsive transcription factor(CARF)may participate in the development of the endometrium by decreasing(P<0.05)the levels of their circRNA-transcript forms.Furthermore,Gene Ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)analyses of DEciR host genes(hgDEciRs)revealed that tight junctions and GTPases may be involved in endometrial development during the estrus cycle.A total of 2331 DEciRs were discovered in the endometrium at Ed5 and Ed15.Based on GO and KEGG enrichment analyses,it could be inferred that tight junctions and GTPases are likely to play an important role in the development of goat endometrium during the estrus cycle.This circRNA study greatly enhances our knowledge of global trends in the development of non-pregnant endometrium during the estrus cycle in goats;these results help us to better understand the molecular regulation of endometrial development in dairy goats.
文摘Background: Malaria infection during pregnancy is a major public health problem in tropical and subtropical regions globally. Anaemia is often an adverse outcome of severe parasitic infections during pregnancy in developing countries. Pregnant women in malaria-endemic communities are more susceptible to Plasmodium falciparum infections than non-pregnant women of child-bearing age. Objective: To comparatively investigate malaria and anaemia in pregnant and non-pregnant women of child-bearing age. Design: A cross-sectional comparative study. Three hundred and eighty pregnant women and 380 non-pregnant women were screened for the study. Setting: The study was conducted at the University Hospital, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana. Measurements: Participants’ demographic data were collected via the administration of questionnaires. In addition their blood samples were analyzed for haemoglobin level and malaria parasites, while stool samples from the pregnant women were examined for intestinal parasites. Results: The study revealed that pregnant women have higher malaria parasitaemia (12.6%) and anaemia (62.6%). The species of Plasmodium isolated from the pregnant women were P. falciparum (85.4%), P. malariae (4.2%) and P. ovale (10.4%). Malaria parasitaemia was higher in the primigravidae (14%). However multigravidae recorded the highest anaemia prevalence (67.1%). Age of pregnant women was a factor affecting malaria parasitaemia with a significant P-value and OR (P value = 0.0041, 0R = 7.61). Conclusions: Pregnant women were more susceptible to malaria and anaemia than non-pregnant women of child-bearing age. Most of the pregnant women reported at antenatal clinic during the second trimester. Primigravidae however recorded the highest malaria parasitaemia. The main species of Plasmodium observed in the blood samples was falciparum.
文摘目的了解孕妇对未来子女非免疫规划疫苗(expanded program on immunization,EPI)的接种意愿及其影响因素,为提高儿童非EPI接种率提供参考。方法采用便利抽样的方法,选取山西省妇幼保健院438名产检孕妇为研究对象进行问卷调查。利用独立样本t检验、方差分析和多重线性回归模型分析个人社会经济因素、疫苗倾向和疫苗信息获取渠道对孕妇为未来子女接种非EPI意愿的影响,并基于计划行为理论构建结构方程模型分析孕妇对子女非EPI接种的态度、主观规范、知觉行为控制对接种意愿的影响。结果98.4%调查对象愿意为子女接种非EPI。与倾向于接种进口疫苗的群体相比,有接种国产疫苗倾向的群体(β=0.119)为子女接种非EPI的意愿更强,有接触媒体宣传较没有接触的群体子女非EPI接种意愿更强(β=0.099),有接触社区医院和疾病预防控制中心的宣传册和活动讲座的群体较没有接触的群体子女非EPI接种意愿更强(β=0.119)。结构方程模型结果显示影响接种意愿强度的因素从大到小依次为知觉行为控制、行为态度、主观规范(路径系数分别为0.73,0.13,0.12)。结论孕妇对未来子女非EPI的接种意愿水平较高。加强非EPI接种宣传教育,改变孕妇对非EPI的知觉行为控制和态度,能够帮助提高儿童非EPI接种率。
