AIM: To investigate reprogramming of human adipose tissue derived stem cells into insulin producing cells using non-integrated lentivirus harboring PDX1 gene.METHODS: In this study, human adipose tissue derived stem c...AIM: To investigate reprogramming of human adipose tissue derived stem cells into insulin producing cells using non-integrated lentivirus harboring PDX1 gene.METHODS: In this study, human adipose tissue derived stem cells(hADSCs) were obtained from abdominal adipose tissues by liposuction, selected by plastic adhesion, and characterized by flow cytometric analysis.Human ADSCs were differentiated into adipocytes and osteocytes using differentiating medium to confirm their multipotency. Non-integrated lentiviruses harboring PDX1(Non-integrated LV-PDX1) were constructed using specific plasmids(pLV-HELP, pMD2G, LV-105-PDX1-1).Then, hADSCs were transduced with non-integrated LVPDX1. After transduction, ADSCsPDX1+were cultured in high glucose DMEM medium supplement by B27, nicotinamide and βFGF for 21 d. Expressions of PDX1 andinsulin were detected at protein level by immunofluorescence analysis. Expressions of PDX1, neurogenin3(Ngn3), glucagon, glucose transporter2(Glut2) and somatostatin as specific marker genes were investigated at mRNA level by quantitative RT-PCR. Insulin secretion of hADSCsPDX1+in the high-glucose medium was detected by electrochemiluminescence test. Human ADSCsPDX1+were implanted into hyperglycemic rats.RESULTS: Human ADSCs exhibited their fibroblast-like morphology and made colonies after 7-10 d of culture.Determination of hADSCs identified by FACS analysis showed that hADSCs were positive for mesenchymal cell markers and negative for hematopoietic cell markers that guaranteed the lack of hematopoietic contamination. In vitro differentiation of hADSCs into osteocytes and adipocytes were detected by Alizarin red and Oil red O staining and confirmed their multilineage differentiation ability. Transduced hADSCs+PDX1became round and clusters in the differentiation medium. The appropriate expression of PDX1 and insulin proteins was confirmed using immunocytochemistry analysis.Significant expressions of PDX1, Ngn3, glucagon, Glut2and somatostatin were detected by quantitative RTPCR. hADSCsPDX1+revealed the glucose sensing ability by expressing Glut2 when they were cultured in the medium containing high glucose concentration. The insulin secretion of hADSCsPDX1+in the high glucose medium was 2.32 μU/mL. hADSCsPDX1+implantation into hyperglycemic rats cured it two days after injection by reducing blood glucose levels from 485 mg/dL to the normal level.CONCLUSION: Human ADSCs can differentiate into IPCs by non-integrated LV-PDX1 transduction and have the potential to be used as a resource in type 1 diabetes cell therapy.展开更多
Studies on first-passage failure are extended to the multi-degree-of-freedom quasi-non-integrable-Hamiltonian systems under parametric excitations of Gaussian white noises in this paper. By the stochastic averaging me...Studies on first-passage failure are extended to the multi-degree-of-freedom quasi-non-integrable-Hamiltonian systems under parametric excitations of Gaussian white noises in this paper. By the stochastic averaging method of energy envelope, the system's energy can be modeled as a one-dimensional approximate diffusion process by which the classical Pontryagin equation with suitable boundary conditions is applicable to analyzing the statistical moments of the first-passage time of an arbitrary order. An example is studied in detail and some numerical results are given to illustrate the above procedure.展开更多
A strategy is proposed based on the stochastic averaging method for quasi non- integrable Hamiltonian systems and the stochastic dynamical programming principle.The pro- posed strategy can be used to design nonlinear ...A strategy is proposed based on the stochastic averaging method for quasi non- integrable Hamiltonian systems and the stochastic dynamical programming principle.The pro- posed strategy can be used to design nonlinear stochastic optimal control to minimize the response of quasi non-integrable Hamiltonian systems subject to Gaussian white noise excitation.By using the stochastic averaging method for quasi non-integrable Hamiltonian systems the equations of motion of a controlled quasi non-integrable Hamiltonian system is reduced to a one-dimensional av- eraged It stochastic differential equation.By using the stochastic dynamical programming princi- ple the dynamical programming equation for minimizing the response of the system is formulated. The optimal control law is derived from the dynamical programming equation and the bounded control constraints.The response of optimally controlled systems is predicted through solving the FPK equation associated with It stochastic differential equation.An example is worked out in detail to illustrate the application of the control strategy proposed.展开更多
The answer of the question was considered as non-integrable basically. The integrable system was thought in the exceptional.Since Poincare had presented in a theorem and proved that non-integrable system is normal, an...The answer of the question was considered as non-integrable basically. The integrable system was thought in the exceptional.Since Poincare had presented in a theorem and proved that non-integrable system is normal, and yet integrable system is abuormal. In the landsliding system, discussed question was regarded to be true.Sortie fundamentals were considered; other systems and example with engineering control are concerned and discussed in details.展开更多
When kernel density is in the class of continuous function to possessing sufficient derivative of high order(and needn't in the class of corresponding Holder function),in this paper it is given the continuity and...When kernel density is in the class of continuous function to possessing sufficient derivative of high order(and needn't in the class of corresponding Holder function),in this paper it is given the continuity and the differential formulas for singular integrals of high non--integral order. The above results themselves and in order to prove in future the formulas to changing order of integration for singular integrals of high non-integral order(another paper) will have important significance. The method to prove in this paper is more different from the method in the corresponding cass of singular integrals of high integral order.展开更多
The Shinto Directive,the official source of the term“State Shinto”,on the one hand defines the concept of“State Shinto”in a narrow sense,and on the other hand reveals its profound connotation and generalized exten...The Shinto Directive,the official source of the term“State Shinto”,on the one hand defines the concept of“State Shinto”in a narrow sense,and on the other hand reveals its profound connotation and generalized extension.In 1945,after the Allied Forces issued the Shinto Directive,Japanese academia carried out in-depth research around“State Shinto”,and gradually formed the“two camps”consisting of scholars who advocated the theory of“State Shinto in a Broad Sense”and insisted on the theory of“State Shinto in a Narrow Sense”.In the process of promoting the democratic reform,the theory of“State Shinto in a Broad Sense”gradually developed into the general theory of“State Shinto”after the war;With the continuous advancement of empirical research,the“State Shinto in a Narrow Sense”gradually rose.While enriching the post-war research of“State Shinto”,it also provided a theoretical basis for historical revisionists to distort and cover up history.Finally,under the situation that the trend of Japanese political right deviation is increasingly intensified,the limitations of the“broad sense”and“narrow sense”camps are broken,and the study of“State Shinto”has entered a new stage.展开更多
基金Supported by National Institute of Genetic Engineering and Biotechnology,Ministry of Science Research and Technology,Tehran,Iran
文摘AIM: To investigate reprogramming of human adipose tissue derived stem cells into insulin producing cells using non-integrated lentivirus harboring PDX1 gene.METHODS: In this study, human adipose tissue derived stem cells(hADSCs) were obtained from abdominal adipose tissues by liposuction, selected by plastic adhesion, and characterized by flow cytometric analysis.Human ADSCs were differentiated into adipocytes and osteocytes using differentiating medium to confirm their multipotency. Non-integrated lentiviruses harboring PDX1(Non-integrated LV-PDX1) were constructed using specific plasmids(pLV-HELP, pMD2G, LV-105-PDX1-1).Then, hADSCs were transduced with non-integrated LVPDX1. After transduction, ADSCsPDX1+were cultured in high glucose DMEM medium supplement by B27, nicotinamide and βFGF for 21 d. Expressions of PDX1 andinsulin were detected at protein level by immunofluorescence analysis. Expressions of PDX1, neurogenin3(Ngn3), glucagon, glucose transporter2(Glut2) and somatostatin as specific marker genes were investigated at mRNA level by quantitative RT-PCR. Insulin secretion of hADSCsPDX1+in the high-glucose medium was detected by electrochemiluminescence test. Human ADSCsPDX1+were implanted into hyperglycemic rats.RESULTS: Human ADSCs exhibited their fibroblast-like morphology and made colonies after 7-10 d of culture.Determination of hADSCs identified by FACS analysis showed that hADSCs were positive for mesenchymal cell markers and negative for hematopoietic cell markers that guaranteed the lack of hematopoietic contamination. In vitro differentiation of hADSCs into osteocytes and adipocytes were detected by Alizarin red and Oil red O staining and confirmed their multilineage differentiation ability. Transduced hADSCs+PDX1became round and clusters in the differentiation medium. The appropriate expression of PDX1 and insulin proteins was confirmed using immunocytochemistry analysis.Significant expressions of PDX1, Ngn3, glucagon, Glut2and somatostatin were detected by quantitative RTPCR. hADSCsPDX1+revealed the glucose sensing ability by expressing Glut2 when they were cultured in the medium containing high glucose concentration. The insulin secretion of hADSCsPDX1+in the high glucose medium was 2.32 μU/mL. hADSCsPDX1+implantation into hyperglycemic rats cured it two days after injection by reducing blood glucose levels from 485 mg/dL to the normal level.CONCLUSION: Human ADSCs can differentiate into IPCs by non-integrated LV-PDX1 transduction and have the potential to be used as a resource in type 1 diabetes cell therapy.
基金The project supported by the Post-Doctoral Foundation of China
文摘Studies on first-passage failure are extended to the multi-degree-of-freedom quasi-non-integrable-Hamiltonian systems under parametric excitations of Gaussian white noises in this paper. By the stochastic averaging method of energy envelope, the system's energy can be modeled as a one-dimensional approximate diffusion process by which the classical Pontryagin equation with suitable boundary conditions is applicable to analyzing the statistical moments of the first-passage time of an arbitrary order. An example is studied in detail and some numerical results are given to illustrate the above procedure.
基金Project supported by the National Natural Science Foundation of China(No.19972059).
文摘A strategy is proposed based on the stochastic averaging method for quasi non- integrable Hamiltonian systems and the stochastic dynamical programming principle.The pro- posed strategy can be used to design nonlinear stochastic optimal control to minimize the response of quasi non-integrable Hamiltonian systems subject to Gaussian white noise excitation.By using the stochastic averaging method for quasi non-integrable Hamiltonian systems the equations of motion of a controlled quasi non-integrable Hamiltonian system is reduced to a one-dimensional av- eraged It stochastic differential equation.By using the stochastic dynamical programming princi- ple the dynamical programming equation for minimizing the response of the system is formulated. The optimal control law is derived from the dynamical programming equation and the bounded control constraints.The response of optimally controlled systems is predicted through solving the FPK equation associated with It stochastic differential equation.An example is worked out in detail to illustrate the application of the control strategy proposed.
文摘The answer of the question was considered as non-integrable basically. The integrable system was thought in the exceptional.Since Poincare had presented in a theorem and proved that non-integrable system is normal, and yet integrable system is abuormal. In the landsliding system, discussed question was regarded to be true.Sortie fundamentals were considered; other systems and example with engineering control are concerned and discussed in details.
文摘When kernel density is in the class of continuous function to possessing sufficient derivative of high order(and needn't in the class of corresponding Holder function),in this paper it is given the continuity and the differential formulas for singular integrals of high non--integral order. The above results themselves and in order to prove in future the formulas to changing order of integration for singular integrals of high non-integral order(another paper) will have important significance. The method to prove in this paper is more different from the method in the corresponding cass of singular integrals of high integral order.
文摘The Shinto Directive,the official source of the term“State Shinto”,on the one hand defines the concept of“State Shinto”in a narrow sense,and on the other hand reveals its profound connotation and generalized extension.In 1945,after the Allied Forces issued the Shinto Directive,Japanese academia carried out in-depth research around“State Shinto”,and gradually formed the“two camps”consisting of scholars who advocated the theory of“State Shinto in a Broad Sense”and insisted on the theory of“State Shinto in a Narrow Sense”.In the process of promoting the democratic reform,the theory of“State Shinto in a Broad Sense”gradually developed into the general theory of“State Shinto”after the war;With the continuous advancement of empirical research,the“State Shinto in a Narrow Sense”gradually rose.While enriching the post-war research of“State Shinto”,it also provided a theoretical basis for historical revisionists to distort and cover up history.Finally,under the situation that the trend of Japanese political right deviation is increasingly intensified,the limitations of the“broad sense”and“narrow sense”camps are broken,and the study of“State Shinto”has entered a new stage.
