We investigated the distribution of four enzymes involved in the immune response of Apostichopus japonicus. We collected samples of the tentacles, papillate podium, integument, respiratory tree, and digestive tract an...We investigated the distribution of four enzymes involved in the immune response of Apostichopus japonicus. We collected samples of the tentacles, papillate podium, integument, respiratory tree, and digestive tract and stained them for acid phosphatase （ACP）, alkaline phosphatase （AKP）, non-specific esterase （NSE） and peroxidase （POD） activity. The distribution and content of ACE AKP, NSE, and POD differed among the tissues. The coelomic epithelium of the tentacle, papillate podium, and integument and the mucous layer of respiratory tree were positive for ACP activity. The coelomic epithelium and cuticular layer of the tentacle, papillate podium, and integument and the mucous layer and tunica externa of the respiratory tree and digestive tract stained positive or weakly positive for AKP activity. Almost all the epithelial tissues stained positive, strongly positive, or very strongly positive for NSE activity. The cuticular layer of the tentacle and integument and the mucous layer, tunica submucosa, and tunica externa of the respiratory tree and digestive tract stained positive for POD activity. We hypothesize that these enzymes play a role in the immune response in A. japonicus.展开更多
The present paper reported the observation of the effect of IUD with indomethacin on enzyme activities of succinic dehydrogenase(SDH),nonspecific esterase(NSE)and ATPase in endomtrium of rabbits,and the comparison wit...The present paper reported the observation of the effect of IUD with indomethacin on enzyme activities of succinic dehydrogenase(SDH),nonspecific esterase(NSE)and ATPase in endomtrium of rabbits,and the comparison with copper intrauterine devices,silicon rubber and IUD with copper and indomethacin.The results indicated:1.In the group of indomethacin,the enzyme activities of SDH,NSE and ATPase were increased,while they were obviously decreased in IUD with copper.In the group of IUD with copper and indomethacin the enzyme activities also decreased,but were higher than those observed in the silicon rubber group.2.The damage to endometrium was obvious in IUD with copper.The damage to epithelium of endometrium in structure and function by IUD with indomethacin was found to be lower.展开更多
基金Supported by the Special Financed Project from the Basic Public Scientific Research Expenses of Central Scientific Institutes (No GY02-2007B03)
文摘We investigated the distribution of four enzymes involved in the immune response of Apostichopus japonicus. We collected samples of the tentacles, papillate podium, integument, respiratory tree, and digestive tract and stained them for acid phosphatase （ACP）, alkaline phosphatase （AKP）, non-specific esterase （NSE） and peroxidase （POD） activity. The distribution and content of ACE AKP, NSE, and POD differed among the tissues. The coelomic epithelium of the tentacle, papillate podium, and integument and the mucous layer of respiratory tree were positive for ACP activity. The coelomic epithelium and cuticular layer of the tentacle, papillate podium, and integument and the mucous layer and tunica externa of the respiratory tree and digestive tract stained positive or weakly positive for AKP activity. Almost all the epithelial tissues stained positive, strongly positive, or very strongly positive for NSE activity. The cuticular layer of the tentacle and integument and the mucous layer, tunica submucosa, and tunica externa of the respiratory tree and digestive tract stained positive for POD activity. We hypothesize that these enzymes play a role in the immune response in A. japonicus.
文摘The present paper reported the observation of the effect of IUD with indomethacin on enzyme activities of succinic dehydrogenase(SDH),nonspecific esterase(NSE)and ATPase in endomtrium of rabbits,and the comparison with copper intrauterine devices,silicon rubber and IUD with copper and indomethacin.The results indicated:1.In the group of indomethacin,the enzyme activities of SDH,NSE and ATPase were increased,while they were obviously decreased in IUD with copper.In the group of IUD with copper and indomethacin the enzyme activities also decreased,but were higher than those observed in the silicon rubber group.2.The damage to endometrium was obvious in IUD with copper.The damage to epithelium of endometrium in structure and function by IUD with indomethacin was found to be lower.
