Objective To study how to promote the smooth innovation and transformation of enterprises and enhance the market competitiveness of enterprises from the perspective of human resources organization development.In the w...Objective To study how to promote the smooth innovation and transformation of enterprises and enhance the market competitiveness of enterprises from the perspective of human resources organization development.In the wave of pharmaceutical industry reform,some entrepreneurs realize the importance of innovation and technology in the future.However,domestic pharmaceutical enterprises are still in the production-oriented stage.In the process of transformative innovation,pharmaceutical enterprises should not only rely on the support of scientific and technological and economic forces,but also need a suitable organizational change and many excellent talents as the source of development.Methods Through retrieving relevant literature and combined with the organizational development practice of X pharmaceutical enterprise,the organizational development model was explored in this paper.Results and Conclusion By forming a system of strategy,organization,talent,culture and mechanism,and building an organizational development model,we can improve the organizational atmosphere and organizational efficiency in the process of enterprise innovation and transformation,so as to enhance the competitiveness of enterprises in the market.展开更多
The plants of hybrid wheatgrass (A. cristatum×A. desertorum cv. Hycrest-Mengnong) were directly induced from embryogenic callus regenerated from immature inflorescence. Immature inflorescence was cultured on im...The plants of hybrid wheatgrass (A. cristatum×A. desertorum cv. Hycrest-Mengnong) were directly induced from embryogenic callus regenerated from immature inflorescence. Immature inflorescence was cultured on improved MS medium containing 2.0-3.0 mg L^-1 2,4-D to regenerate callus. The calli were then transferred to hormone-free MS medium for differentiation and 1/2 MS medium for rooting. Results showed that callus initiation frequency was 83.4% and plant regeneration frequency was 59.6%. Phosphinothricin acetyltransferase (bar) gene was transformed into the hybrid wheatgrass by particle bombardment. Resistant callus was obtained using selecting agent, herbicide glufosinate of 0.5 mg L^-1, and some transgenic plants were recovered in vitro. The transgenic plants were identified by PCR and Southern blot analysis and these plants developed normally in the glufosinate medium, whereas the nontransgenic plants did not. The results demonstrated that bar cDNA integrated into the genomic DNA of the transgenic plants. The transgenic frequencies of bar gene were 1.1%.展开更多
Vitis vinifera cv.Chardonnay suspension cultures were established from proembryogenic mass and employed for optimizing Agrobacterium-mediated transformation system.One-factor-at-a-time experiment revealed that OD600 o...Vitis vinifera cv.Chardonnay suspension cultures were established from proembryogenic mass and employed for optimizing Agrobacterium-mediated transformation system.One-factor-at-a-time experiment revealed that OD600 of Agrobacterium,time of inoculation,co-cultivation,and cell-drying before inoculation significantly affected the transformation efficiency which reached maximum 21.5% at the following conditions:0.8 of OD600,25 min of inoculation,2 d of co-cultivation,and 10 min of cell drying.Response surface methodology experiments based on a five-level,four-factor central-composite rotatable design were then used to optimize these selected factors.The optimized conditions for Chardonnay grape transformation were:0.8711 of OD600,28.9 min of inoculation,2.25 d of co-cultivation and 11.76 min of cell drying.After optimization,transformation efficiency was 26.2% and there were no interactions among different factors.展开更多
The 'double T-DNA' binary vector p13HSR which harbored two independent T-DNAs, containing hygromycin phosphotransferase gene (hpf) in one T-DNA region and three target genes (hLF, SB401, RZ10) in another T-DNA r...The 'double T-DNA' binary vector p13HSR which harbored two independent T-DNAs, containing hygromycin phosphotransferase gene (hpf) in one T-DNA region and three target genes (hLF, SB401, RZ10) in another T-DNA region, was used to generate selectable marker-free transgenic rice by Agrobacterium-mediated transformation. The regenerated plants with both the three target genes and the selectable marker gene hpt were selected for anther culture. RT-PCR analysis indicated that target genes were inserted in rice genomic DNA and successfully transcribed. It took only one year to obtain double haploid selectable marker-free transgenic plants containing the three target genes with co-transformation followed by anther culture technique, and the efficiency was 12.2%. It was also noted that one or two target genes derived from the binary vector were lost in some transgenic rice plants.展开更多
Bacopa monnieri (L.) Wettst. (Scrophulariaceae) is a highly sought after medicinal plant with therapeutic properties as cognition enhancer as well as for other brain and body functions. Research was conducted to optim...Bacopa monnieri (L.) Wettst. (Scrophulariaceae) is a highly sought after medicinal plant with therapeutic properties as cognition enhancer as well as for other brain and body functions. Research was conducted to optimize a thin cell layer explant based micropropagation system to assist mass propagation. Thin cell layers (TCL) derived from leaf and internode segments were used as explants. Murashige and Skoog medium was used to formulate shoot induction, elongation, and rooting media. Shoot induction media were prepared by supplementing three concentrations (0.1, 1.0, and 10.0 μM) of four cytokinins 6-benzylaminopurine, 2-isopentenyl-adenine, 6-3-Hydroxybenzylaminopurine, and thidiazuron to study adventitious shoot bud induction response. An optimum shoot bud induction response was observed on MS medium supplemented with 10.0 μM 6-benzylaminopurine for both leaf and stem transverse thin cell layer (tTCL) explants. The average number of shoot buds from leaf tTCL explants was 59, whereas, on an average, 33 shoot buds were regenerated from internode tTCL explants. Elongation of adventitious shoot buds was achieved best in a liquid medium using Liquid Lab Rocker<sup>®</sup> system. Elongated shoots recorded 100% rooting in MS medium supplemented with 5 μM indole butyric acid. Bacopa micropropagation employing tTCL explants for initial shoot bud induction and using LLR<sup>®</sup> boxes in subsequent elongation step can achieve cost effective way to regenerate high volume of plantlets and biomass required for herbal industry. Leaf and stem tTCL explants both were suitable for Agrobacterium tumefaciens (EHA105) mediated genetic transformation. Successful transformation was scored within three days of co-cultivation with Agrobacterium suspension on the basis of Enhanced Green Fluorescent Protein (EGFP) expression as an early and non-destructible screening device. Transformation frequencies of 83% and 76% were accomplished for leaf and stem tTCL explants, respectively. Greenhouse grown Bacopa plants were analyzed as fresh and dry methanolic extracts for total polyphenol content (811.93 ± 7.98 and 814 ± 17.64 GAE mg g-1) and the Trolox Equivalent Antioxidant Capacity values were 1918.25 ± 173.12 and 3163.14 ± 403.25 μmol/g, respectively.展开更多
The transformation and governance of urban villages with historic culture is faced with difficulties such as lack of attention and large-scale demolition and construction,which is not conducive to the continuation of ...The transformation and governance of urban villages with historic culture is faced with difficulties such as lack of attention and large-scale demolition and construction,which is not conducive to the continuation of urban characteristic culture.Taking Xiaxifang,Yongfeng County,Ji'an City,Jiangxi Province for example,this study explored the transformation and governance of urban villages with historic culture from the perspectives of spatial structure,urban function,infrastructure,ecological environment,and community governance,so as to provide a reference for the research and practice of urban villages with historic culture.展开更多
With the development of planta genetic engineering, more emphases have been laid on convenient and high efficient genetic transformation methods. And transformation without tissue culture is a prospective direction of...With the development of planta genetic engineering, more emphases have been laid on convenient and high efficient genetic transformation methods. And transformation without tissue culture is a prospective direction of it. In this paper, traditional transformation methods and the methods of non-tissue culture were summarized. With the exploration and application of Arabidopsis transformation mechanism, with the use of ion beam-mediated transfor- mation invented by Chinese scientists and the development of other transformation methods, transformation methods without tissue culture and planta genetic engineering could be improved rapidly.展开更多
AIM:To evaluate the differences between human lacrimal gland adenoid cystic carcinoma with high-grade transformation(LACC-HGT)primar y cells cultured by high-grade transformation tissue and non-high-grade transformati...AIM:To evaluate the differences between human lacrimal gland adenoid cystic carcinoma with high-grade transformation(LACC-HGT)primar y cells cultured by high-grade transformation tissue and non-high-grade transformation(non-HGT)primary cells cultured by non-highgrade transformation tissue in proliferation,metastasis,drug susceptibility,and genes.METHODS:LACC-HGT primary cells were established by tissue block culture,and the 4^(th)to 10^(th)generation primary cells were selected as research objects.The cells were preliminarily identified by immunofluorescent staining.The differences between non-HGT and LACC-HGT primary cells in terms of proliferation,metastasis,and drug susceptibility were compared by cell counting kit-8(CCK-8)assay,wound healing,and drug sensitivity experiments.Differentially expressed genes were screened using mRNA array.Gene expression was analyzed using real-time quantitative polymerase chain reaction(RT-qPCR).RESULTS:LACC-HGT primary cells were successfully cultured by tissue block culture.Immunofluorescence staining results showed that cytokeratin(CK)and CK7 expression levels were positive in LACC-HGT primary cells.CCK-8 results showed that the proliferation ability of LACCHGT cells was significantly higher than that of non-HGT cells.Wound healing experiment showed that the migration ability of LACC-HGT cells was significantly higher than that of non-HGT cells.LACC-HGT cells were also less sensitive to cisplatin and paclitaxel than non-HGT cells.Compared with non-HGT cells,9566 differentially expressed genes were found in LACC-HGT primary cells,of which 5162 were upregulated and 4404 were down-regulated.The expression of N-acetylneuraminate pyruvate lyase(NPL),MARVEL domain containing 3(MARVELD3),syntabulin(SYBU),and allograft inflammatory factor 1(AIF1)was higher in LACCHGT cells than in non-HGT cells,whereas that of periostin(POSTN)was lower.CONCLUSION:LACC-HGT primary cells have faster proliferation,stronger migration ability,and poorer sensitivity to chemotherapy drugs than non-HGT primary cells.The expression of mRNAs in non-HGT and LACC-HGT primary cells are significantly different.These features are speculated to be the reasons why high-grade transformation tissues exhibit higher malignant degree and poorer prognosis than their counterparts.展开更多
This paper examines transformational leadership theory and the relationship between transformational leadership and group performance as well. Transformational leadership asks leaders to understand the needs of follow...This paper examines transformational leadership theory and the relationship between transformational leadership and group performance as well. Transformational leadership asks leaders to understand the needs of followers and motivate followers for their overall development, which brings benefits to a group. It is hoped that this paper can contribute to comparative studies on Transformational leadership in the U.S. and China.展开更多
Summary: In order to investigate whether cultured normal human lens epithelial cells (LEC) express transforming growth factor β (TGF-β), reverse transcriptase polymerase chain reaction (RT-PCR) and immunohistochemic...Summary: In order to investigate whether cultured normal human lens epithelial cells (LEC) express transforming growth factor β (TGF-β), reverse transcriptase polymerase chain reaction (RT-PCR) and immunohistochemical methods were used for detection of TGF-β mRNA and protein in cultured normal human LEC. The results showed that a single RT-PCR amplified product about 310bp was obtained, and the sequence was homologous to the known sequence. TGF-β immunostain was positive in the plasma of LEC. It was suggested that normal human LEC could produce TGF-β, and LEC could be affected by TGF-β through autocrine action.展开更多
From the perspective of social transformation,this paper analyzed the actual value of the construction of Wenzhou rural cultural auditorium,and it was found that it has important value in promoting the transformation ...From the perspective of social transformation,this paper analyzed the actual value of the construction of Wenzhou rural cultural auditorium,and it was found that it has important value in promoting the transformation of rural culture,economy,and grassroots governance.The construction of rural cultural auditorium is helpful for improving the present backward status of rural public cultural service supply,and promoting the integration and development of rural culture and urban culture;cultivating new rural economic entities,injecting action force for rural economic transformation and development;providing space guarantee for citizen participation in rural grassroots social governance,and is favorable for enhancing the awareness of public participation in rural grassroots governance.However,in the context of comprehensive rural revitalization,the value of cultural auditorium construction has not yet been fully realized.It is necessary to further enhance its value in rural social transformation from three aspects:auditorium construction,daily activity undertaking,and residents'participation in cultivation.展开更多
As a way for carrying forward tradition,heritage constitutes a unique interpretation and expression of human culture with its richness and integrity.However,with economic globalization and the rapid development of sci...As a way for carrying forward tradition,heritage constitutes a unique interpretation and expression of human culture with its richness and integrity.However,with economic globalization and the rapid development of science and technology,while enjoying the benefits brought by modernity,human beings have also noticed that the traditions and cultures represented by heritage have been impacted and dissolved to a certain extent in the world.展开更多
The effect of transforming growth factor β 2 (TGF β 2) on phagocytosis in bovine trabecular meshwork cells in vitro was investigated. After the cultured bovine trabecular meshwork cells were treated with 0 ...The effect of transforming growth factor β 2 (TGF β 2) on phagocytosis in bovine trabecular meshwork cells in vitro was investigated. After the cultured bovine trabecular meshwork cells were treated with 0 ng/ml, 0.32 ng/ml, 1 ng/ml, 3.2 ng/ml TGF β 2 for 24 h, latex beads were added into the incubation medium, and the numbers of the latex beads in 20 adjacent cells were counted under a microscope 24 h later, after treatment with Wright's stain. Our results showed that the average numbers of the latex beads in the trabecular meshwork cells treated with TGF β 2 of different concentrations were 53.1±1.7 beads/cell, 56.4±2.9 beads/cell and 77.9±6.5 beads/cell respectinvely, in comparison with 45.5±3.3 beads/cell of the control group. TGF β 2 significantly increased the number of the latex beads phagocytosed by cultured bovine trabecular meshwork cells in a dose dependent manner. TGF β 2 could promote the phagocytosis of bovine trabecular meshwork cells in vitro . It may be involved in the cellularity decrease of the trabecular meshwork in the patients of primary open angle glaucoma through promoting the phagocytosis of trabecular meshwork cells.展开更多
The era of cultural sharing marks itself mainly in the following three aspects: First, the virtual presence which bridges the world together and further broadens our horizons to observe the world in an even more exten...The era of cultural sharing marks itself mainly in the following three aspects: First, the virtual presence which bridges the world together and further broadens our horizons to observe the world in an even more extensive way. Second, the formerly stable structural relationship between peripheral regions and central regions gradually grows uncertain. Third, the establishment of cyberized roads or the cyberized expansion of roads leads the advance toward a global connectivity where the virtual world closely interacts with the real world. After "the Belt" concept comes into being, the anthropologists must upgrade their research from previously established fixed-point perspective to a more extensive observation over the "Belt" or a wider range, while the concept of "the Road" encourages anthropologists to study the culture of the other half of the world, namely, to read the world history as a whole new picture from a Chinese or eastern point of view. It is necessary for anthropology to break the geological and cultural borders in the real world to extend its antenna to a more extensive range, which will also bring the "otherness" of this field to a more prominent and obvious play. It is inevitable that all human cultures are about to take a core standing in the Belt and Road Initiative.展开更多
In order to make foreign tourists familiar with China's scenic spots, it is necessary that tourist publicity materials are properly translated. Cultural dimension, as one of the three key dimensions of eco-transla...In order to make foreign tourists familiar with China's scenic spots, it is necessary that tourist publicity materials are properly translated. Cultural dimension, as one of the three key dimensions of eco-translatology, plays a very important role in translating. With its basis on cultural dimension of eco- translatology, this paper aims to present transformation techniques of parataxis and hypotaxis between Chinese and English. It is suggested that, with the cultural dimensional transformation techniques of parataxis and hypotaxis, translators should exert their subjectivity and creativity to achieve in maximizing the degree of holistic adaptation and selection for achieving successful translations.展开更多
Human rights refer to the rights that should be enjoyed by anyone as human beings. However, human beings have not only the natural at- tributes, but also social attributes, including such high-level social at- tribute...Human rights refer to the rights that should be enjoyed by anyone as human beings. However, human beings have not only the natural at- tributes, but also social attributes, including such high-level social at- tributes as thinking capability and culture. The role of culture on hu- man beings can be seen in people's understandings on human rights connotation and human rights value. In fact, the different views on hu- man rights issue of various countries worldwide are linked with their dif- ferent cultures. We cannot change the differences of various countries in cultures, but we can learn about and understand the differences through cultural exchanges so as to enhance recognition on human rights issue.展开更多
Genetic transformation of rice(Oryza sativa L.) by introducing beneficial traits is now a central research instrument in plant physiology and a practical tool for plant improvement. Many approaches are verified for st...Genetic transformation of rice(Oryza sativa L.) by introducing beneficial traits is now a central research instrument in plant physiology and a practical tool for plant improvement. Many approaches are verified for stable introduction of foreign genes into the plant genome. The review examined the different constraints that limit the success of rice genetic transformation via Agrobacterium-mediated approach and suggested possible solutions. Explant identification, gene transfer technique and construct to tailor the integration, transgene expression without collateral to genetic damage and transformant selection are among the technical challenges affecting the rice transformation. Despite the contests, Agrobacteriummediated transformation system has been a better option for producing transgenic rice varieties because of its exact T-DNA processing and simple integration of low copy-number transgene. This information is necessary for improving the transformation system for recalcitrant rice varieties.展开更多
文摘Objective To study how to promote the smooth innovation and transformation of enterprises and enhance the market competitiveness of enterprises from the perspective of human resources organization development.In the wave of pharmaceutical industry reform,some entrepreneurs realize the importance of innovation and technology in the future.However,domestic pharmaceutical enterprises are still in the production-oriented stage.In the process of transformative innovation,pharmaceutical enterprises should not only rely on the support of scientific and technological and economic forces,but also need a suitable organizational change and many excellent talents as the source of development.Methods Through retrieving relevant literature and combined with the organizational development practice of X pharmaceutical enterprise,the organizational development model was explored in this paper.Results and Conclusion By forming a system of strategy,organization,talent,culture and mechanism,and building an organizational development model,we can improve the organizational atmosphere and organizational efficiency in the process of enterprise innovation and transformation,so as to enhance the competitiveness of enterprises in the market.
文摘The plants of hybrid wheatgrass (A. cristatum×A. desertorum cv. Hycrest-Mengnong) were directly induced from embryogenic callus regenerated from immature inflorescence. Immature inflorescence was cultured on improved MS medium containing 2.0-3.0 mg L^-1 2,4-D to regenerate callus. The calli were then transferred to hormone-free MS medium for differentiation and 1/2 MS medium for rooting. Results showed that callus initiation frequency was 83.4% and plant regeneration frequency was 59.6%. Phosphinothricin acetyltransferase (bar) gene was transformed into the hybrid wheatgrass by particle bombardment. Resistant callus was obtained using selecting agent, herbicide glufosinate of 0.5 mg L^-1, and some transgenic plants were recovered in vitro. The transgenic plants were identified by PCR and Southern blot analysis and these plants developed normally in the glufosinate medium, whereas the nontransgenic plants did not. The results demonstrated that bar cDNA integrated into the genomic DNA of the transgenic plants. The transgenic frequencies of bar gene were 1.1%.
基金supported by the 948 Program,Ministry of Agriculture,China(2006-G26)the National Grape Industry Technology System,China(nycytx-30-zy-05)
文摘Vitis vinifera cv.Chardonnay suspension cultures were established from proembryogenic mass and employed for optimizing Agrobacterium-mediated transformation system.One-factor-at-a-time experiment revealed that OD600 of Agrobacterium,time of inoculation,co-cultivation,and cell-drying before inoculation significantly affected the transformation efficiency which reached maximum 21.5% at the following conditions:0.8 of OD600,25 min of inoculation,2 d of co-cultivation,and 10 min of cell drying.Response surface methodology experiments based on a five-level,four-factor central-composite rotatable design were then used to optimize these selected factors.The optimized conditions for Chardonnay grape transformation were:0.8711 of OD600,28.9 min of inoculation,2.25 d of co-cultivation and 11.76 min of cell drying.After optimization,transformation efficiency was 26.2% and there were no interactions among different factors.
文摘The 'double T-DNA' binary vector p13HSR which harbored two independent T-DNAs, containing hygromycin phosphotransferase gene (hpf) in one T-DNA region and three target genes (hLF, SB401, RZ10) in another T-DNA region, was used to generate selectable marker-free transgenic rice by Agrobacterium-mediated transformation. The regenerated plants with both the three target genes and the selectable marker gene hpt were selected for anther culture. RT-PCR analysis indicated that target genes were inserted in rice genomic DNA and successfully transcribed. It took only one year to obtain double haploid selectable marker-free transgenic plants containing the three target genes with co-transformation followed by anther culture technique, and the efficiency was 12.2%. It was also noted that one or two target genes derived from the binary vector were lost in some transgenic rice plants.
文摘Bacopa monnieri (L.) Wettst. (Scrophulariaceae) is a highly sought after medicinal plant with therapeutic properties as cognition enhancer as well as for other brain and body functions. Research was conducted to optimize a thin cell layer explant based micropropagation system to assist mass propagation. Thin cell layers (TCL) derived from leaf and internode segments were used as explants. Murashige and Skoog medium was used to formulate shoot induction, elongation, and rooting media. Shoot induction media were prepared by supplementing three concentrations (0.1, 1.0, and 10.0 μM) of four cytokinins 6-benzylaminopurine, 2-isopentenyl-adenine, 6-3-Hydroxybenzylaminopurine, and thidiazuron to study adventitious shoot bud induction response. An optimum shoot bud induction response was observed on MS medium supplemented with 10.0 μM 6-benzylaminopurine for both leaf and stem transverse thin cell layer (tTCL) explants. The average number of shoot buds from leaf tTCL explants was 59, whereas, on an average, 33 shoot buds were regenerated from internode tTCL explants. Elongation of adventitious shoot buds was achieved best in a liquid medium using Liquid Lab Rocker<sup>®</sup> system. Elongated shoots recorded 100% rooting in MS medium supplemented with 5 μM indole butyric acid. Bacopa micropropagation employing tTCL explants for initial shoot bud induction and using LLR<sup>®</sup> boxes in subsequent elongation step can achieve cost effective way to regenerate high volume of plantlets and biomass required for herbal industry. Leaf and stem tTCL explants both were suitable for Agrobacterium tumefaciens (EHA105) mediated genetic transformation. Successful transformation was scored within three days of co-cultivation with Agrobacterium suspension on the basis of Enhanced Green Fluorescent Protein (EGFP) expression as an early and non-destructible screening device. Transformation frequencies of 83% and 76% were accomplished for leaf and stem tTCL explants, respectively. Greenhouse grown Bacopa plants were analyzed as fresh and dry methanolic extracts for total polyphenol content (811.93 ± 7.98 and 814 ± 17.64 GAE mg g-1) and the Trolox Equivalent Antioxidant Capacity values were 1918.25 ± 173.12 and 3163.14 ± 403.25 μmol/g, respectively.
文摘The transformation and governance of urban villages with historic culture is faced with difficulties such as lack of attention and large-scale demolition and construction,which is not conducive to the continuation of urban characteristic culture.Taking Xiaxifang,Yongfeng County,Ji'an City,Jiangxi Province for example,this study explored the transformation and governance of urban villages with historic culture from the perspectives of spatial structure,urban function,infrastructure,ecological environment,and community governance,so as to provide a reference for the research and practice of urban villages with historic culture.
基金Supported by "863 Programme" (2002AA327070) and Key Technologies R&D Programme (2001BA302B-03)
文摘With the development of planta genetic engineering, more emphases have been laid on convenient and high efficient genetic transformation methods. And transformation without tissue culture is a prospective direction of it. In this paper, traditional transformation methods and the methods of non-tissue culture were summarized. With the exploration and application of Arabidopsis transformation mechanism, with the use of ion beam-mediated transfor- mation invented by Chinese scientists and the development of other transformation methods, transformation methods without tissue culture and planta genetic engineering could be improved rapidly.
基金Supported by the Tianjin Key Medical Discipline(Specialty)Construction Project(No.TJYXZDXK-037A)Tianjin Medical University Eye Hospital。
文摘AIM:To evaluate the differences between human lacrimal gland adenoid cystic carcinoma with high-grade transformation(LACC-HGT)primar y cells cultured by high-grade transformation tissue and non-high-grade transformation(non-HGT)primary cells cultured by non-highgrade transformation tissue in proliferation,metastasis,drug susceptibility,and genes.METHODS:LACC-HGT primary cells were established by tissue block culture,and the 4^(th)to 10^(th)generation primary cells were selected as research objects.The cells were preliminarily identified by immunofluorescent staining.The differences between non-HGT and LACC-HGT primary cells in terms of proliferation,metastasis,and drug susceptibility were compared by cell counting kit-8(CCK-8)assay,wound healing,and drug sensitivity experiments.Differentially expressed genes were screened using mRNA array.Gene expression was analyzed using real-time quantitative polymerase chain reaction(RT-qPCR).RESULTS:LACC-HGT primary cells were successfully cultured by tissue block culture.Immunofluorescence staining results showed that cytokeratin(CK)and CK7 expression levels were positive in LACC-HGT primary cells.CCK-8 results showed that the proliferation ability of LACCHGT cells was significantly higher than that of non-HGT cells.Wound healing experiment showed that the migration ability of LACC-HGT cells was significantly higher than that of non-HGT cells.LACC-HGT cells were also less sensitive to cisplatin and paclitaxel than non-HGT cells.Compared with non-HGT cells,9566 differentially expressed genes were found in LACC-HGT primary cells,of which 5162 were upregulated and 4404 were down-regulated.The expression of N-acetylneuraminate pyruvate lyase(NPL),MARVEL domain containing 3(MARVELD3),syntabulin(SYBU),and allograft inflammatory factor 1(AIF1)was higher in LACCHGT cells than in non-HGT cells,whereas that of periostin(POSTN)was lower.CONCLUSION:LACC-HGT primary cells have faster proliferation,stronger migration ability,and poorer sensitivity to chemotherapy drugs than non-HGT primary cells.The expression of mRNAs in non-HGT and LACC-HGT primary cells are significantly different.These features are speculated to be the reasons why high-grade transformation tissues exhibit higher malignant degree and poorer prognosis than their counterparts.
文摘This paper examines transformational leadership theory and the relationship between transformational leadership and group performance as well. Transformational leadership asks leaders to understand the needs of followers and motivate followers for their overall development, which brings benefits to a group. It is hoped that this paper can contribute to comparative studies on Transformational leadership in the U.S. and China.
文摘Summary: In order to investigate whether cultured normal human lens epithelial cells (LEC) express transforming growth factor β (TGF-β), reverse transcriptase polymerase chain reaction (RT-PCR) and immunohistochemical methods were used for detection of TGF-β mRNA and protein in cultured normal human LEC. The results showed that a single RT-PCR amplified product about 310bp was obtained, and the sequence was homologous to the known sequence. TGF-β immunostain was positive in the plasma of LEC. It was suggested that normal human LEC could produce TGF-β, and LEC could be affected by TGF-β through autocrine action.
基金Program of"Xinmiao"(Potential)Talents in Zhejiang Province"Research on the Development Status of Volunteer Service in Zhejiang Province"(2019R429058).
文摘From the perspective of social transformation,this paper analyzed the actual value of the construction of Wenzhou rural cultural auditorium,and it was found that it has important value in promoting the transformation of rural culture,economy,and grassroots governance.The construction of rural cultural auditorium is helpful for improving the present backward status of rural public cultural service supply,and promoting the integration and development of rural culture and urban culture;cultivating new rural economic entities,injecting action force for rural economic transformation and development;providing space guarantee for citizen participation in rural grassroots social governance,and is favorable for enhancing the awareness of public participation in rural grassroots governance.However,in the context of comprehensive rural revitalization,the value of cultural auditorium construction has not yet been fully realized.It is necessary to further enhance its value in rural social transformation from three aspects:auditorium construction,daily activity undertaking,and residents'participation in cultivation.
文摘As a way for carrying forward tradition,heritage constitutes a unique interpretation and expression of human culture with its richness and integrity.However,with economic globalization and the rapid development of science and technology,while enjoying the benefits brought by modernity,human beings have also noticed that the traditions and cultures represented by heritage have been impacted and dissolved to a certain extent in the world.
基金This project was supported by a grant from the NationalNatural Science Foundation of China (No.38970 75 8)
文摘The effect of transforming growth factor β 2 (TGF β 2) on phagocytosis in bovine trabecular meshwork cells in vitro was investigated. After the cultured bovine trabecular meshwork cells were treated with 0 ng/ml, 0.32 ng/ml, 1 ng/ml, 3.2 ng/ml TGF β 2 for 24 h, latex beads were added into the incubation medium, and the numbers of the latex beads in 20 adjacent cells were counted under a microscope 24 h later, after treatment with Wright's stain. Our results showed that the average numbers of the latex beads in the trabecular meshwork cells treated with TGF β 2 of different concentrations were 53.1±1.7 beads/cell, 56.4±2.9 beads/cell and 77.9±6.5 beads/cell respectinvely, in comparison with 45.5±3.3 beads/cell of the control group. TGF β 2 significantly increased the number of the latex beads phagocytosed by cultured bovine trabecular meshwork cells in a dose dependent manner. TGF β 2 could promote the phagocytosis of bovine trabecular meshwork cells in vitro . It may be involved in the cellularity decrease of the trabecular meshwork in the patients of primary open angle glaucoma through promoting the phagocytosis of trabecular meshwork cells.
基金Initial achievement for the participation of ethnic regions in the strategic research of the Belt and Road Initiative under the background of cultural transformation(2016-GMA-004)a research project on ethnic issues under the State Ethnic Affairs Commission(2016)
文摘The era of cultural sharing marks itself mainly in the following three aspects: First, the virtual presence which bridges the world together and further broadens our horizons to observe the world in an even more extensive way. Second, the formerly stable structural relationship between peripheral regions and central regions gradually grows uncertain. Third, the establishment of cyberized roads or the cyberized expansion of roads leads the advance toward a global connectivity where the virtual world closely interacts with the real world. After "the Belt" concept comes into being, the anthropologists must upgrade their research from previously established fixed-point perspective to a more extensive observation over the "Belt" or a wider range, while the concept of "the Road" encourages anthropologists to study the culture of the other half of the world, namely, to read the world history as a whole new picture from a Chinese or eastern point of view. It is necessary for anthropology to break the geological and cultural borders in the real world to extend its antenna to a more extensive range, which will also bring the "otherness" of this field to a more prominent and obvious play. It is inevitable that all human cultures are about to take a core standing in the Belt and Road Initiative.
文摘In order to make foreign tourists familiar with China's scenic spots, it is necessary that tourist publicity materials are properly translated. Cultural dimension, as one of the three key dimensions of eco-translatology, plays a very important role in translating. With its basis on cultural dimension of eco- translatology, this paper aims to present transformation techniques of parataxis and hypotaxis between Chinese and English. It is suggested that, with the cultural dimensional transformation techniques of parataxis and hypotaxis, translators should exert their subjectivity and creativity to achieve in maximizing the degree of holistic adaptation and selection for achieving successful translations.
文摘Human rights refer to the rights that should be enjoyed by anyone as human beings. However, human beings have not only the natural at- tributes, but also social attributes, including such high-level social at- tributes as thinking capability and culture. The role of culture on hu- man beings can be seen in people's understandings on human rights connotation and human rights value. In fact, the different views on hu- man rights issue of various countries worldwide are linked with their dif- ferent cultures. We cannot change the differences of various countries in cultures, but we can learn about and understand the differences through cultural exchanges so as to enhance recognition on human rights issue.
文摘Genetic transformation of rice(Oryza sativa L.) by introducing beneficial traits is now a central research instrument in plant physiology and a practical tool for plant improvement. Many approaches are verified for stable introduction of foreign genes into the plant genome. The review examined the different constraints that limit the success of rice genetic transformation via Agrobacterium-mediated approach and suggested possible solutions. Explant identification, gene transfer technique and construct to tailor the integration, transgene expression without collateral to genetic damage and transformant selection are among the technical challenges affecting the rice transformation. Despite the contests, Agrobacteriummediated transformation system has been a better option for producing transgenic rice varieties because of its exact T-DNA processing and simple integration of low copy-number transgene. This information is necessary for improving the transformation system for recalcitrant rice varieties.