Objective:To investigate the prevalence of non-tuberculosis mycobacteria(NTM)among the samples deposited from the National Tuberculosis Reference Laboratory of Iran between 2011 and 2018.Methods:The study evaluated th...Objective:To investigate the prevalence of non-tuberculosis mycobacteria(NTM)among the samples deposited from the National Tuberculosis Reference Laboratory of Iran between 2011 and 2018.Methods:The study evaluated the prevalence of NTM among specimens from patients with pulmonary tuberculosis symptoms(n=15771)deposited at the National Tuberculosis Reference Laboratory of Iran from 2011 to 2018.Detection of Mycobacterium(M.)tuberculosis was based on presence of a 190-bp amplicon from IS6110 insertion sequence using Tb1 and Tb2 primers,and amplicon-negative specimens were tested for NTM and M.tuberculosis(refractory to IS6110 amplification)using restriction fragment length polymorphism PCR of hsp65 amplicon fragment.Results:A total of 7307(46.33%)M.tuberculosis and 658(4.17%)NTM specimens were found,the latter mainly comprising M.abscessus(10.18%),M.avium(2.28%),M.chelonae(8.97%),M.intracellulare(10.49%),M.kansasii(4.71%),and M.simiae(56.08%).Conclusions:As treatment for NTM differs from that for M.tuberculosis,accurate detection of Mycobacterium sp.is of public health significance.展开更多
The incidence of Mycobacterium avium complex pulmonary disease (MACPD) has been globally increasing. The present study aims to provide a comprehensive analysis of clinical characteristics and treatment response among ...The incidence of Mycobacterium avium complex pulmonary disease (MACPD) has been globally increasing. The present study aims to provide a comprehensive analysis of clinical characteristics and treatment response among patients in the Amazon region of Brazil and conduct a retrospective cohort study at a prominent referral hospital in the State of Par, Brazil, from 2012 to 2020. The M. avium group represented 58.9% of cases, followed by M. intracellulare (35.7%), M. colombiense (3.6%), and M. chimaera (1.8%). The majority of patients (73.2%) were female and with an average age of 63 years. Primary clinical manifestations included persistent cough and sputum production. The Charlson comorbidity index (CCI) averaged 2.47, with 36.3% of patients having a CCI score equal to higher than 3. Notably, over 70% of patients had received treatment for pulmonary tuberculosis before initiating treatment for MAC infection. The fibrocavitary radiologic form was the most prevalent (55.4%), frequently exhibiting a bilateral distribution (53.6%). Antimicrobial susceptibility revealed a significant prevalence of MAC resistance to drugs in the therapeutic regimen. Despite this, most of the patients experienced clinical improvement (50%). In conclusion, this study highlights a higher prevalence of MAC infections among middle-aged women, with a history of pulmonary tuberculosis treatment and presenting the fibrocavitary radiological form was predominant.展开更多
基金supported by funding from the Mycobacteriology Research Center,National Tuberculosis and Lung Research Institute(NRITLD),Masih Daneshvari Hospital(Grant No.16597)
文摘Objective:To investigate the prevalence of non-tuberculosis mycobacteria(NTM)among the samples deposited from the National Tuberculosis Reference Laboratory of Iran between 2011 and 2018.Methods:The study evaluated the prevalence of NTM among specimens from patients with pulmonary tuberculosis symptoms(n=15771)deposited at the National Tuberculosis Reference Laboratory of Iran from 2011 to 2018.Detection of Mycobacterium(M.)tuberculosis was based on presence of a 190-bp amplicon from IS6110 insertion sequence using Tb1 and Tb2 primers,and amplicon-negative specimens were tested for NTM and M.tuberculosis(refractory to IS6110 amplification)using restriction fragment length polymorphism PCR of hsp65 amplicon fragment.Results:A total of 7307(46.33%)M.tuberculosis and 658(4.17%)NTM specimens were found,the latter mainly comprising M.abscessus(10.18%),M.avium(2.28%),M.chelonae(8.97%),M.intracellulare(10.49%),M.kansasii(4.71%),and M.simiae(56.08%).Conclusions:As treatment for NTM differs from that for M.tuberculosis,accurate detection of Mycobacterium sp.is of public health significance.
文摘The incidence of Mycobacterium avium complex pulmonary disease (MACPD) has been globally increasing. The present study aims to provide a comprehensive analysis of clinical characteristics and treatment response among patients in the Amazon region of Brazil and conduct a retrospective cohort study at a prominent referral hospital in the State of Par, Brazil, from 2012 to 2020. The M. avium group represented 58.9% of cases, followed by M. intracellulare (35.7%), M. colombiense (3.6%), and M. chimaera (1.8%). The majority of patients (73.2%) were female and with an average age of 63 years. Primary clinical manifestations included persistent cough and sputum production. The Charlson comorbidity index (CCI) averaged 2.47, with 36.3% of patients having a CCI score equal to higher than 3. Notably, over 70% of patients had received treatment for pulmonary tuberculosis before initiating treatment for MAC infection. The fibrocavitary radiologic form was the most prevalent (55.4%), frequently exhibiting a bilateral distribution (53.6%). Antimicrobial susceptibility revealed a significant prevalence of MAC resistance to drugs in the therapeutic regimen. Despite this, most of the patients experienced clinical improvement (50%). In conclusion, this study highlights a higher prevalence of MAC infections among middle-aged women, with a history of pulmonary tuberculosis treatment and presenting the fibrocavitary radiological form was predominant.
